scholarly journals Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Qiyun Yang ◽  
Wanmei Chen ◽  
Chi Zhang ◽  
Yun Xie ◽  
Yong Gao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Erectile Dysfunction ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Rat Model ◽  
Erectile Function ◽  
Normal Group ◽  
Endothelial Progenitor ◽  
Intracavernous Injection

Erectile dysfunction (ED) is a common complication in men suffered with diabetic mellitus. Stem cell transplantation is a promising strategy for the treatment of diabetic ED (DED). In this study, we evaluated whether combined transplantation of adipose tissue-derived stem cells (ADSCs) and endothelial progenitor cells (EPCs) could improve the erectile function of the DED rat model. DED rats were induced via intraperitoneal injection of streptozotocin (50 mg/kg), and ED was screened by apomorphine (100 mg/kg). DED rats were divided into 4 groups (n=14 each): DED, ADSC, EPC, and ADSC/EPC group. Another 14 age-matched male SD rats with normal erectile function were served as the normal group. The normal group and the DED group were received intracavernous injection with phosphate-buffered saline (PBS). And the other groups were received intracavernous injection with ADSCs (1×106), EPCs (1×106), and ADSCs/EPCs (0.5×106/0.5×106), respectively. The total intracavernous pressure (ICP) and mean arterial pressure (MAP) were recorded at day 28 after injection. The endothelium, smooth muscle, and penile dorsal nerves were assessed within cavernoursal tissue. On day 28 after injection, the ADSC/EPC group displayed more significantly enhanced ICP and ICP/MAP than the DED or ADSC or EPC group (p<0.05). Immunofluorescent analysis and western blot demonstrated that the improvement of erectile function in the ADSC/EPC5 group was associated with increased expression of endothelial marker (CD31) and the correction of eNOS-cGMP-NO signaling. More 5-ethynyl-2′-deoxyuridine- (EdU-) positive EPCs could be found lining in the cavernous endothelial layer in the ADSC/EPC group than the EPC group, which was attributed to the paracrine of vascular endothelial growth factor (VEGF) and stromal-derived factor-1 (SDF-1) by ADSCs. Combined transplantation of ADSCs and EPCs has a synergic effect in repairing the endothelial function of DED rats, and the underlying mechanism might be the paracrine of VEGF and SDF-1 by ADSCs, which improves the recruitment and proliferation of EPCs in the cavernosum.

scholarly journals The Effect of Intracavernous Injection of Adipose Tissue-Derived Stem Cells on Hyperlipidemia-Associated Erectile Dysfunction in a Rat Model

2010 ◽  
Vol 7 (4) ◽  
pp. 1391-1400 ◽  
Author(s):  
Yun-Ching Huang ◽  
Hongxiu Ning ◽  
Alan W. Shindel ◽  
Thomas M. Fandel ◽  
Guiting Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Erectile Dysfunction ◽  
Rat Model ◽  
Intracavernous Injection

scholarly journals Human Endothelial Progenitor Cells as a Novel Therapeutic Option to Treat Severe Erectile Dysfunction in Obese Type 2 Diabetic Rats

2016 ◽  
Vol 9 (1) ◽  
pp. 152-152
Author(s):  
P. Ganesan Adaikan ◽  
◽  
A. Al Mahmood Siddiquee ◽  
L. Chu Lau ◽  
B. Bin Said ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Cord Blood ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Erectile Function ◽  
Dependent Manner ◽  
Type Ii ◽  
Endothelial Progenitor ◽  
Zdf Rats

Erectile dysfunction (ED) is a consequence of a number of medical conditions, with a high prevalence among men with type II diabetes. We investigated the effect of intracavernous injection of human umbilical cord blood derived endothelial progenitor cells (EPCs) on ED in a type II diabetic rat model. Erectile function was first assessed by cavernous nerve electrostimulation in Zucker Diabetic Fat (ZDF) rats aged 24 weeks to establish the baseline intracavernous pressure (ICP). At the age of 28 weeks, severe ED was confirmed. ZDF rats at age 29 weeks were randomised into 3 groups: treatment with 1million and 2million EPCs, and control, PBS. The injected animals were maintained with daily cyclosporine (10mg/kg) for 4 weeks, followed by the evaluation of erectile function and immunohistochemistry of penile tissue. The maximum (mean±SD) ICP observed for EPCs treated animals were significantly higher, 24.7±7.4 and 42.1±5.2 mmHg for 1million and 2million EPCs respectively in comparison to 4.4±1.6 mmHg in the age-matched PBS treated animals (p<0.001). EPCs treatment also improved the smooth muscle content of the cavernosum (p<0.001) and the expression of eNOS, RECA-1 and nNOS (p<0.05) compared to the PBS control. In conclusion, we showed for the first time that human cord blood derived EPCs significantly improved severe ED in ZDF rats in a dose-dependent manner through improvement of the nerve and endothelium function and also through preservation of smooth muscle cells in the cavernosum.

scholarly journals Isolation of endothelial progenitor cells from human adipose tissue

2016 ◽  
Vol 3 (05) ◽  
pp. 645-652 ◽  
Author(s):  
Phuc Van Pham ◽  
Ngoc Bich Vu ◽  
Hoa Trong Nguyen ◽  
Ngoc Kim Phan
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Human Adipose Tissue ◽  
Vascular Medicine ◽  
Endothelial Progenitor ◽  
Adipose Tissues ◽  
Surface Marker Expression

Adipose tissue is a rich source of stem cells, especially mesenchymal stem cells (MSCs). This study aimed to identify and isolate endothelial progenitor cells (EPCs) from human adipose tissue. Belly adipose tissues were collected from donors with consent. Stromal vascular fractions (SVFs) were extracted from adipose tissues by enzyme collagenase using commercial kits. SVFs were cultured in MSCCult medium for 24 h to obtain MSCs, then supernatant was collected and cell pellet cultured in EGM-2 medium to obtain adipose tissue EPCs (ADEPCs). ADEPCs were checked for surface marker expression of CD31 and VEGFR2, and for angiogenesis capability in vitro. The results showed that SVFs contained a pool of EPCs with strong angiogenesis potential and that adipose tissue is not only a source for MSCs but also for EPCs. Therefore, ADEPCs may a useful source of EPCs for vascular medicine.

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