scholarly journals Increased Mast Cell Counts and Degranulation in Microscopic Colitis

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Zhikai Chi ◽  
Jing Xu ◽  
Romil Saxena
Keyword(s):  
Mast Cell ◽  
Cell Count ◽  
Cell Activation ◽  
Collagenous Colitis ◽  
Microscopic Colitis ◽  
Mast Cell Activation ◽  
Mucosal Inflammation ◽  
High Power Field ◽  
Cell Counts ◽  
Mast Cell Count

Objectives. Microscopic colitis (MC) is characterized by chronic diarrhea, normal colonoscopy findings, and mucosal inflammation in colonic biopsies and can be classified as collagenous colitis (CC) or lymphocytic colitis (LC). However, the pathogenesis of MC is largely unknown. In this study, we aimed to study mast cell counts and activation in MC. Methods. We investigated 64 biopsy samples from the surgical pathology database of Indiana University Health, which met the diagnostic criteria for CC or LC along with 20 control samples collected from 2014 to 2015. The specimens were used for the quantification of mast cells by examining the presence of intracellular and extracellular tryptase by immunohistochemistry. Results. In the lamina propria, the mast cell count was higher in both CC and LC groups than the control (mean highest count, 39/high-power field (HPF) vs. 30/HPF vs. 23/HPF; P<0.01). Extracellular tryptase was present in 10% of control subjects as compared to 41% of CC (P<0.05) and 60% of LC (P<0.001) patients. When LC patients were stratified into two groups with either <80% or >80% of fragments affected by inflammation, increased mast cell counts are only observed in the >80% involvement group compared with the control, but not the <80% involvement group. Conclusions. The increased mast cell count and degranulation are identified in MC, suggesting that mast cell activation might be involved in the pathogenesis of MC.

scholarly journals Correlation between clinical findings, mast cell count and interleukin 31 immunostaining in the skin of dogs with atopic dermatitis

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Bárbara Hess Rodrigues Gonçalves ◽  
Bruna Dantas Matos ◽  
Mariana Batista Rodrigues Faleiro ◽  
Emmanuel Arnhold ◽  
Moema Pacheco Chediak Matos ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Count ◽  
Plasma Cells ◽  
Clinical Severity ◽  
Cell Numbers ◽  
Cell Counts ◽  
Clinical Scores ◽  
Mast Cell Count

ABSTRACT: In this study the correlation between the clinical score, mast cell count and interleukin 31 (IL-31) immunostaining in the skin of dogs with atopic dermatitis was determined. A total of 31 dogs of different breeds, from one to eight years of age, were chosen for the study. The 20 females and 11 males were categorized based on the CADESI-4 system, as having discrete, moderate or marked atopic dermatitis. Skin samples were collected from the axillary and interdigital regions and stained with hematoxylin and eosin for cytohistomorphological analyses and toluidine blue to evaluate the mast cell counts, and immunohistochemistry for the IL-31 immunostaining. Animals revealing higher atopic dermatitis scores had greater numbers of mast cells and IL-31 immunolabeled cells. More numbers of cells immunolabeled for IL-31 were evident in the axillary skin compared with the interdigital skin in dogs having this condition. A correlation was identified between the clinical scores and mast cell numbers in the interdigital region, as well as between the clinical scores and number of cells immunolabeled for IL-31 in the axillary area. A correlation was also reported between the mast cell numbers and IL-31 immunolabeled cells only in the axillary skin, and none in the interdigital regions. It was thus concluded that the mast cells and IL-31 are involved in the pathogenesis of the canine atopic dermatitis (CAD), as well as lymphocytes and plasma cells. It was also observed that the higher the degree of clinical severity of the disease, the more the numbers of mast cells and IL-31 in the skin of those animals suffering from CAD, which implies the influence of these immunological constituents on the genesis of pruritus and disease progression.

THE OXYTOCIN-INACTIVATING ACTIVITY OF THE RAT UTERUS

1972 ◽  
Vol 52 (3) ◽  
pp. 451-457
Author(s):  
M. N. BURJORJEE ◽  
U. MALHOTRA ◽  
R. R. CHAUDHURY
Keyword(s):  
Mast Cell ◽  
Cell Count ◽  
Cell Population ◽  
Intrauterine Devices ◽  
Rat Uterus ◽  
Cell Counts ◽  
Mast Cell Count ◽  
Series Of Experiments ◽  
Mast Cell Population ◽  
Intact Rats

SUMMARY The oxytocin-inactivating activity (OIA) of rat uterus homogenates was studied in intact animals and in animals with bilateral intrauterine devices (IUD). In another series of experiments the OIA of the rat uterus was related to the mast cell count of uteri from intact rats and from rats with bilateral intrauterine devices. The OIA of the homogenates was significantly higher at oestrus than at dioestrus. No such increase was observed in homogenates from rats at oestrus with bilateral IUD's. The IUD caused an increase in the mast cell population at oestrus and the increase in mast cell counts observed in uterine homogenates of intact rats at dioestrus was not observed in the presence of a device. No correlation between the OIA of uterine homogenates and the mast cell population was observed in animals with or without IUD at oestrus or dioestrus.

Evidence for mast cell activation in collagenous colitis

1998 ◽  
Vol 47 (0) ◽  
pp. 64-65 ◽  
Author(s):  
D. Schwab ◽  
M. Raithel ◽  
E. G. Hahn
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Collagenous Colitis ◽  
Mast Cell Activation

Depression, psychosocial correlates, and psychosocial resources in individuals with mast cell activation syndrome

2021 ◽  
pp. 135910532110145
Author(s):  
Jennifer Nicoloro SantaBarbara ◽  
Marci Lobel
Keyword(s):  
Mast Cell ◽  
Depressive Symptoms ◽  
Cell Activation ◽  
Physical Symptoms ◽  
Mast Cell Activation ◽  
Emotional States ◽  
Mast Cell Activation Syndrome ◽  
Disease Experience ◽  
Clinically Significant ◽  
Activation Syndrome

Individuals with Mast Cell Activation Syndrome (MCAS), a rare chronic disease, experience unpredictable physical symptoms and diagnostic challenges resulting in poor emotional states. The prevalence and correlates of depressive symptoms were examined among 125 participants who completed the CES-D and relevant instruments. The majority reported a clinically-significant level of depression which was especially common among younger participants and those who reported greater loneliness or more disease-specific stressors. Greater magnitude of depressive symptoms was associated with greater illness intrusiveness, less social support, and lower optimism. Results highlight the value of interventions targeting loneliness and stressors unique to this population.

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