scholarly journals Upregulation of ECT2 Predicts Adverse Clinical Outcomes and Increases 5-Fluorouracil Resistance in Gastric Cancer Patients

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hua Zhang ◽  
Yuan Geng ◽  
Chunhui Sun ◽  
Jin Yu

Background. The abnormal expression and prognosis prediction of epithelial cell transforming sequence 2 (ECT2) in gastric cancer (GC) has been reported. However, the effect of ECT2 on 5-fluorouracil (5-Fu) resistance in GC is unclear. This research aims to solve the abovementioned problems. Methods. Gene expression was detected by RT-qPCR and Western blot analysis. Cell viability was evaluated by the colony formation assay, MTT assay, and flow cytometric analysis. Transwell and wound healing assays were used to detect cell metastasis. Results. Upregulation of ECT2 was found in stomach adenocarcinoma (STAD) and GC tissues. In addition, high ECT2 expression can predict adverse clinical outcomes in GC patients. More importantly, ECT2 knockdown weakened the resistance of 5-FU in GC cells. ECT2 silencing reduced the cell migratory and invasive abilities of GC cells treated with 5-FU. We also found that downregulation of ECT2 increased 5-FU sensitivity in GC cells by downregulating P-gp, MRP1, and Bcl-2. Conclusion. Upregulation of ECT2 can predict adverse clinical outcomes and increase 5-FU resistance in GC patients.

1987 ◽  
Vol 56 (1) ◽  
pp. 52-54 ◽  
Author(s):  
KC Ballantyne ◽  
PD James ◽  
RA Robins ◽  
RW Baldwin ◽  
JD Hardcastle

2018 ◽  
Author(s):  
Dipjit Basak ◽  
Pronoy Kanti Mondal ◽  
Brishti Mondal ◽  
Ranajoy Ghosh ◽  
Aniket Halder ◽  
...  

2020 ◽  
Vol 21 (11) ◽  
pp. 751-759
Author(s):  
Wei Li ◽  
Weili Wang ◽  
Ping Liao ◽  
Kun Song ◽  
Zhanwei Zhu ◽  
...  

Background: Tumor-infiltrating lymphocytes (TILs) and postoperative chemotherapeutics interact in the tumor micro-environment. This interaction has not been well investigated in gastric cancer. Materials & methods: A total of 129 patients were divided into high or low TILs based on the median number of positive CD3+ and FoxP3+ T cells, which was assessed by immunocytochemistry. Results: Cox regression analysis showed that the stage III disease with shorter overall survival was significant. The analysis showed that high numbers of CD3+ or FoxP3+ T cells have better clinical outcomes in FOLFOX-treated patients. Conclusion: High CD3+ and FoxP3+ T-cell infiltration was associated with better clinical outcomes in patients with gastric cancer treated with FOLFOX, suggesting TILs incorporated into algorithms to improve the therapeutic efficacy of optimal chemotherapy.


2021 ◽  
Vol 10 (1) ◽  
pp. 1915560
Author(s):  
Kaifeng Jin ◽  
Yifan Cao ◽  
Yun Gu ◽  
Hanji Fang ◽  
Yuchao Fei ◽  
...  

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