scholarly journals Inactivated Nevus Tissue with High Hydrostatic Pressure Treatment Used as a Dermal Substitute after a 28-Day Cryopreservation Period

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yoshitaka Matsuura ◽  
Michiharu Sakamoto ◽  
Shuichi Ogino ◽  
Jun Arata ◽  
Naoki Morimoto
Keyword(s):  
Complete Removal ◽  
Basement Membranes ◽  
Elastic Fibers ◽  
Skin Defect ◽  
Dermal Substitute ◽  
Fresh Tissue ◽  
Significant Damage ◽  
Giant Congenital Melanocytic Nevi ◽  
Congenital Melanocytic Nevi ◽  
Cultured Epidermis

Background. Giant congenital melanocytic nevi (GCMN) treatment remains controversial. While surgical resection is the best option for complete removal, skin shortage to reconstruct the skin defect remains an issue. We report a novel treatment using a high hydrostatic pressurization (HHP) technique and a cryopreservation procedure. However, cryopreservation may inhibit revascularization of implanted nevus tissue and cultured epidermal autograft (CEA) take. We aimed to investigate the influence of the cryopreservation procedure on the HHP-treated dermis specimen and CEA take on cryopreserved tissue. Methods. Nevus tissue harvested from a patient with GCMN was inactivated with HHP of 200 MPa and then cryopreserved at -30°C for 28 days. The cryopreserved specimen was compared with fresh (HHP-treated without cryopreservation) tissue and with untreated (without HHP treatment) tissue to evaluate the extracellular matrix, basal membranes, and capillaries. Cultured epidermis (CE) take on the cryopreserved tissue was evaluated following implantation of the cryopreserved nevus tissue with CE into the subcutis of nude mice. Results. No difference was observed between cryopreserved and fresh tissue in terms of collagen or elastic fibers, dermal capillaries, or basement membranes at the epidermal-dermal junction. In 4 of 6 samples (67%), applied CE took on the nevus tissues and regenerated the epidermis in the cryopreserved group compared with 5 of 6 samples (83%) in the fresh group. Conclusion. Cryopreservation at -30°C for 28 days did not result in significant damage to inactivated nevus tissue, and applied CE on the cryopreserved nevus tissues took and regenerated the epidermis. Inactivated nevus tissue with HHP can be used as a dermal substitute after 28-day cryopreservation.

scholarly journals S-100B serum protein is elevated in children with medium-to-giant congenital melanocytic nevi: An exploratory case-control study

2020 ◽  
Vol 83 (1) ◽  
pp. 222-224
Author(s):  
Alejandra Tomás-Velázquez ◽  
Juan Carlos López-Gutiérrez ◽  
Carmen Ceballos ◽  
Jorge M. Núñez-Córdoba ◽  
Pedro Redondo
Keyword(s):  
Serum Protein ◽  
Case Control Study ◽  
Case Control ◽  
Melanocytic Nevi ◽  
Giant Congenital Melanocytic Nevi ◽  
Congenital Melanocytic Nevi ◽  
Control Study

Giant Congenital Melanocytic Nevi

2008 ◽  
Vol 47 (8) ◽  
pp. 824-826 ◽  
Author(s):  
Ahdi Amer ◽  
Howard Fischer
Keyword(s):  
Melanocytic Nevi ◽  
Giant Congenital Melanocytic Nevi ◽  
Congenital Melanocytic Nevi

scholarly journals V. THE IRON CONTENT OF BLOOD FREE TISSUES AND VISCERA

1932 ◽  
Vol 55 (4) ◽  
pp. 653-665 ◽  
Author(s):  
Robert P. Bogniard ◽  
George H. Whipple
Keyword(s):  
Iron Content ◽  
Striated Muscle ◽  
Complete Removal ◽  
The Body ◽  
Iron Store ◽  
Fresh Tissue ◽  
Iron Storage ◽  
Excess Iron ◽  
Tissue Edema ◽  
The Individual

When hemoglobin is set free in the circulation the kidney plays an important part in the conservation of iron. When the renal threshold is not exceeded by the hemoglobin in the blood there is little or no excess iron deposited in the kidney but when superthreshold doses of blood hemoglobin are given the epithelium of the convoluted tubules accumulates much iron and the iron analyses may show 5 times normal values. The normal dog (140 to 150 per cent hemoglobin) has a large reserve store of iron in the liver, spleen and marrow. Diets may modify this storage of iron in these tissues. To bring conclusive proof relating to the individual diet factors, the reserve store of iron should be depleted by an anemia period of 2 to 3 months. Complete removal of red cells from tissue capillaries is essential for accurate iron assays of fresh tissue. The method described accomplishes this without causing gross tissue edema. The lowest iron content is observed in the pancreas, stomach, jejunum, colon and urinary bladder. These figures average from 1 to 2 mg. iron per 100 gm. fresh tissue. This shows that smooth muscle and mucous membranes contain little iron. Striated muscle (heart, psoas) shows a relatively low iron content but uniform values close to 4 mg. per 100 gm. tissue. Lungs show a considerable fluctuation with low iron values in anemia (3.7 mg.) and higher values in health (6 to 7 mg.). The spleen shows maximal fluctuations and the highest reserve storage of iron per 100 gm. fresh tissue. The spleen iron analyses show low values in anemia (7 to 15 mg.) and wide differences in controls (25 to 50 mg.). With hemoglobin injections the iron storage is conspicuous and iron analyses may run as high as l50 to 175 mg. iron per 100 gm. fresh tissue. Bone marrow of the rib runs in parallel with the spleen as regards iron storage following hemoglobin injections and depletion following anemia periods. The liver because of its weight always contains the main bulk of the iron stored in the blood free tissues of the body. Its store is depleted by anemia even to levels of 4 to 5 mg. iron per 100 gm. fresh tissue. In the normal dog the iron store in the liver averages 25 mg. per 100 gm. tissue. Frequent hemoglobin injections may increase this level to 31 mg. iron per 100 gm. The liver is considered the most active clearing house for iron storage and utilization.

scholarly journals Multiple giant congenital nevi – a case report / Multipli gigantski kongenitalni nevusi – prikaz slučaja

2011 ◽  
Vol 3 (2) ◽  
pp. 69-76
Author(s):  
Vesna Mikulić
Keyword(s):  
Body Surface ◽  
Psychosocial Problems ◽  
The Body ◽  
Skin Tumor ◽  
Whole Body ◽  
Neurocutaneous Melanosis ◽  
Melanocytic Nevi ◽  
Increased Risk ◽  
Giant Congenital Melanocytic Nevi ◽  
Congenital Melanocytic Nevi

Abstract Giant congenital melanocytic nevi are benign nevomelanocytic proliferations of 20 cm or more in diameter, present at birth. They are primarily found on the posterior trunk, but they may arise on any other part of the body, covering more than 2% of the body surface. Giant congenital nevi are major risk factors for the development of melanoma, and the risk has been estimated to be as high as 5-7%. Persons with giant congenital melanocytic nevi on the head, neck and along the midline of the back are at increased risk for leptomeningeal melanocytic lesions. Most patients with neurocutaneous melanosis present with neurologic manifestations of the disease in the first 2 years of life. Melanoma occurs in 62-80% of cases, but even without neoplasms, symptomatic neurocutaneous melanosis has a poor prognosis. This is a report of a 23-year-old female patient who presented with multiple congenital pigmented and pilous nevi covering over 2% of her total body surface, without malignant alterations or association with other abnormalities. At birth, a nevus covered her neck, shoulders and the upper left arm, whereas several nevi over 5cm in diameter were present in the gluteal region, on the abdomen and legs. During the first 2 years of life, the existing nevi increased in size and progressed into darker brown. New, smaller pigmented changes appeared on the whole body and the face, while at the age of 17 they reached their current size and layout. At puberty, nevi over 10cm in size grew dark hairs. There were neither melanoma nor skin tumor cases in the family. Nuclear magnetic resonance imaging was not performed in the childhood or later in life, but other parameters - neurologic and ophthalmologic findings were in normal range all the time, as was growth and development. A complete photo-documentation was made, including macroscopic and dermoscopic images and regular follow-ups continue. Giant congenital melanocytic nevi may cause considerable esthetic and psychosocial problems. Due to their high malignant potential, association with other abnormalities, no consensus on the treatment, and monitoring problems, giant congenital melanocytic nevi represent a therapeutic problem as well.

Neurocutaneous Melanosis with Giant Congenital Melanocytic Nevi

2016 ◽  
Vol 14 (01) ◽  
pp. 047-050
Author(s):  
Shaukat Ali ◽  
Saifullah Khalid ◽  
Kashif Azmi ◽  
Murad Ahmad ◽  
Shaad Abqari
Keyword(s):  
Neurocutaneous Melanosis ◽  
Melanocytic Nevi ◽  
Giant Congenital Melanocytic Nevi ◽  
Congenital Melanocytic Nevi
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