scholarly journals Green, Cost-Effective Simultaneous Assay of Chloramphenicol, Methylparaben, and Propylparaben in Eye-Drops by Capillary Zone Electrophoresis

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Thi Thanh Vuong Tong ◽  
Thi Thoa Cao ◽  
Nguyen Ha Tran ◽  
Thi Kim Van Le ◽  
Dinh Chi Le
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Background Electrolyte ◽  
Cost Effective ◽  
Complete Separation ◽  
Sodium Tetraborate ◽  
Eye Drops ◽  
Solution Ph ◽  
Zone Electrophoresis ◽  
Capillary Zone

A green, cost-effective, and simple capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of chloramphenicol, methylparaben, and propylparaben in eye-drops. With sodium tetraborate as background electrolyte (BGE), the apparent mobilities of chloramphenicol, methylparaben, and propylparaben increased and analysis time reduced when pH of BGE increased from 8.5 to 10.0 and concentration of BGE decreased from 40 mM to 15 mM, but complete separation of chloramphenicol from other matrix components was achieved only with sodium tetraborate concentration at 30 mM or higher and at pH = 9.3 or lower. The most suitable electrophoretic conditions for the intended application were a 30 mM sodium tetraborate solution, pH 9.3 as BGE, working voltage set at 25 kV, and UV detection at 280 nm at the cathodic extremity of the capillary. The final method was validated and proved to be reliable for assay of chloramphenicol, methylparaben, and propylparaben in eye-drops.

scholarly journals Determination of aristolochic acid by capillary zone electrophoresis

2004 ◽  
Vol 2 (3) ◽  
pp. 417-424 ◽  
Author(s):  
František Kvasnička ◽  
Rudolf Ševčík ◽  
Michal Voldřich ◽  
Jana Krátká
Keyword(s):  
Detection Limit ◽  
Capillary Zone Electrophoresis ◽  
Aristolochic Acid ◽  
Background Electrolyte ◽  
Ease Of Use ◽  
Clear Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Running Cost

AbstractA simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.

scholarly journals Determination of phenolic acids by capillary zone electrophoresis and HPLC

2008 ◽  
Vol 6 (3) ◽  
pp. 410-418 ◽  
Author(s):  
František Kvasnička ◽  
Jana Čopíková ◽  
Rudolf Ševčík ◽  
Jana Krátká ◽  
Andrej Syntytsia ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Phenolic Acids ◽  
Background Electrolyte ◽  
Aminocaproic Acid ◽  
Chlorogenic Acids ◽  
Barley Malt ◽  
Phase Gradient ◽  
Zone Electrophoresis ◽  
Capillary Zone

AbstractSelected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods’ characteristics were: linearity (1–20 mg ml and 0.2–4 mg ml−1), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability (4.1% and 3.5%), and detection limit (0.2 and 0.02 mg ml−1).

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