Objective To study the effect of Zedoary turmeric oil on proliferation and apoptosis of human gastric cancer cell line SGC-7901. The growth inhibition rate of different concentration of Zedoary turmeric oil on human gastric cancer cell line SGC-7901 were detected by trypan blue stain assay. Optical microscopy observation of cell morphological changes. DNA fragmentation detected by agarose gel electrophoresis. Flow cytometry was used to detect the changes of cell mitochondrial membrane potential, apoptosis rate and cell cycle distribution.Results: the optimal concentration was110 μg/mL for 48h,and the IC50 values were 104.958 μg/mL. The typical DNA ladder on agarose gel electrophoresis,the apoptotic peak was showed by FCA detection. Conclusion: The Zedoary turmeric oil can induce apoptosis of gastric cancer cell SGC-7901.