Metabolic Aggressiveness in Benign Meningiomas with Chromosomal Instabilities

Barrett’s esophagus (BE) is categorized, based on morphological appearance, into different stages, which correlate with the risk of developing esophageal adenocarcinoma. More advanced stages are more likely to acquire chromosomal instabilities, but stage-specific markers remain elusive. Here, we performed single-cell DNA-sequencing experiments (scDNAseq) with fresh BE biopsies. Dysplastic BE cells frequently contained chromosomal instability (CIN) regions, and these CIN cells carried mutations corresponding to the COSMIC mutational signature SBS17, which were not present in biopsy-matched chromosomally stable (CS) cells or patient-matched nondiseased control cells. CS cells were predominantly found in nondysplastic BE biopsies. The single-base substitution (SBS) signatures of all CS BE cells analyzed were indistinguishable from those of nondiseased esophageal or gastric cells. Single-cell RNA-sequencing (scRNAseq) experiments with BE biopsies identified two sets of marker genes which facilitate the distinction between columnar BE epithelium and nondysplastic/dysplastic stages. Moreover, histological validation confirmed a correlation between increased CLDN2 expression and the presence of dysplastic BE stages. Our scDNAseq and scRNAseq datasets, which are a useful resource for the community, provide insight into the mutational landscape and gene expression pattern at different stages of BE development.

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Chromosomal Abnormalities in Endometrial and Ovarian Carcinomas

Balkan Journal of Medical Genetics ◽

10.2478/v10034-008-0008-y ◽

2007 ◽

Vol 10 (2) ◽

pp. 61-70 ◽

Cited By ~ 1

Author(s):

A Pazarbaşi ◽

M Kasap ◽

O Demirhan ◽

M Vardar ◽

D Suleymanova-Karahan ◽

...

Keyword(s):

Ovarian Cancer ◽

Chromosomal Abnormalities ◽

Banding Technique ◽

Giemsa Banding ◽

Specific Chromosome ◽

Ovarian Carcinomas ◽

Genetic Changes ◽

Cytogenetic Studies ◽

Chromosomal Instabilities ◽

Chromosome Bands

Chromosomal Abnormalities in Endometrial and Ovarian CarcinomasDevelopment and progression of human malignancies involve multiple genetic changes including chromosomal instabilities such as translocations, deletions, and inversions. Chromosomal abnormalities were observed in 23 cases with ovarian and endometrial cancer by cytogenetic studies using a GTG (G bands by trypsin using Giemsa) banding technique. Specific chromosome bands were frequently involved, and were most frequent on chromosomes 1, 2, 3, 5, 12 and 17. Clonal alterations were observed at the cancer breakpoints, such as 1q21, 1q32, 3p21, 7q22, 11q23 in ovarian and 1p36, 1q32, 2p12, 3p21, 7q22, 9q34, 11p15, 11q23, 12q13, 14q11, 14q32, 16p13, 21q22 in endometrial cases. These findings provide evidence that multiple genetic lesions are associated with the pathogenesis of endometrial and ovarian cancer.

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Chromosomal instabilities in callus tissue from haploid barley (Hordeum vulgare L.)

Biologia Plantarum ◽

10.1007/bf02892778 ◽

1980 ◽

Vol 22 (4) ◽

pp. 303-305 ◽

Cited By ~ 4

Author(s):

F. J. Novák

Keyword(s):

Hordeum Vulgare ◽

Callus Tissue ◽

Hordeum Vulgare L ◽

Chromosomal Instabilities

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MITOTIC INSTABILITIES IN TETRAPLOID, HEXAPLOID, AND OCTOPLOID BROMUS INERMIS

Canadian Journal of Genetics and Cytology ◽

10.1139/g77-056 ◽

1977 ◽

Vol 19 (3) ◽

pp. 531-536 ◽

Cited By ~ 11

Author(s):

Geok-Yong Tan ◽

G. M. Dunn

Keyword(s):

Somatic Cells ◽

Morphological Characters ◽

Bromus Inermis ◽

Root Tip ◽

Smooth Bromegrass ◽

High Frequencies ◽

Anaphase Bridges ◽

Tip Cells ◽

Chromosomal Instabilities ◽

Root Tip Cells

Mitotic irregularities were observed in the root tip cells at three ploidies, tetraploid (4x), hexaploid (6x), and octoploid (8x), of smooth bromegrass (Bromus inermis Leyss.). These included endomitosis, fragments, chromosomes excluded from the spindle, anaphase bridges, laggards and micronuclei, at different stages of mitosis. High frequencies of anaphase irregularities were detected at the 4x and 6x levels. The commercial octoploid was more stable. Intraplant variations of some morphological characters in smooth bromegrass may be attributed to chromosomal instabilities in somatic cells.

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