Abstract 2458: Genetically induced cancer stem cells (CSCs) in mouse mammary gland from Ink4a/Arf-knockout mammary cells transduced with H-rasG12V

2010 ◽  
Author(s):  
Kazuharu Kai ◽  
Hideyuki Saya ◽  
Gabriel N. Hortobagyi ◽  
Naoto T. Ueno
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Cancer Stem Cells ◽  
Mouse Mammary Gland ◽  
Mammary Cells

scholarly journals Metabolism of the chemopreventive retinoid N-(4-hydroxyphenyl)retinamide by mammary gland in organ culture

1988 ◽  
Vol 256 (2) ◽  
pp. 579-584 ◽  
Author(s):  
R G Mehta ◽  
T A Hultin ◽  
R C Moon
Keyword(s):  
Mammary Gland ◽  
Organ Culture ◽  
Steroid Hormones ◽  
Hormonal Regulation ◽  
Mammary Carcinogenesis ◽  
Reversed Phase ◽  
Mouse Mammary Gland ◽  
Mammary Cells ◽  
Chemically Induced ◽  
Chloroform Methanol

N-(4-Hydroxyphenyl)retinamide (4-HPR) is considered to be the most effective chemopreventive retinoid for chemically induced mammary carcinogenesis in rats. However, the mechanism of 4-HPR action in mammary cells is poorly understood. In the present study we examined the metabolism of 4-HPR in the mouse mammary gland in organ culture. Mammary glands excised from BALB/c mice were incubated with 4-HPR in the presence of insulin, prolactin and steroid hormones for 6 days. The glands were extracted with chloroform/methanol (2:1, v/v), and the metabolites were separated on a reversed-phase h.p.l.c. column. Three metabolites were separated in addition to 4-HPR; one of the metabolites, M2, was co-eluted with 13-cis-4-HPR, M3 was co-eluted with N-(4-methoxyphenyl)retinamide (4-MPR) and M1 remains unidentified. There appeared to be some hormonal regulation in the distribution of metabolites in the glands. Increased levels of 4-MPR and M1 were observed in insulin-plus-prolactin-treated glands as compared with the glands incubated with steroid hormones. Furthermore, it was observed that M1 isolated from the livers of 4-HPR-treated rats competed for the cellular retinoic acid-binding protein (CRABP) sites; however, 4-HPR did not bind to CRABP. These results indicate that mouse mammary gland can metabolize 4-HPR and that the metabolites which compete for CRABP sites may have physiological significance in the retinoid inhibition of mammary carcinogenesis.

Progesterone generates cancer stem cells through membrane progesterone receptor-triggered signaling in basal-like human mammary cells

2015 ◽  
Vol 362 (2) ◽  
pp. 167-173 ◽  
Author(s):  
Guillaume Vares ◽  
Sei Sai ◽  
Bing Wang ◽  
Akira Fujimori ◽  
Mitsuru Nenoi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Progesterone Receptor ◽  
Mammary Cells ◽  
Membrane Progesterone Receptor

scholarly journals Crosstalks Among Cancer Stem Cells and Histopathologic Features in Determining Prognosis in Canine Mammary Gland Carcinomas

2021 ◽  
Vol 71 (3) ◽  
pp. 303-317
Author(s):  
Havva Marzban ◽  
Gelareh Chamani ◽  
Fariba Khaki ◽  
Parnian Amini ◽  
Nastaran Pedram ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Lymph Node ◽  
Cancer Stem Cells ◽  
Vasculogenic Mimicry ◽  
Lymph Node Status ◽  
Mesenchymal Transition ◽  
Node Status ◽  
Canine Mammary Gland ◽  
Histopathologic Features

Abstract The purpose of the present work was the evaluation of the prognostic potential of histopathologic features, cancer stem cells (CSCs), and epthelial-mesenchymal transition (EMT) in relation to lymph node status and lymphovascular invasion (LVI) in canine mammary gland carcinomas (CMGCs). CSCs are proposed as the main cause of tumorigenesis, therapy failure, and recurrence which form a small fraction of tumor bulk. We evaluated presence of micropapillary growth pattern (MGP), infiltration into surrounding tissues (IST), and vasculogenic mimicry (VM) in H&E stained slides of 26 paraffin-embedded tumor samples. Lymph nodes of all cases were assessed. Additionally, they were examined immunohistochemically in terms of vimentin expression as an indicator of EMT which is a well-known mechanism for metastasis, and CD44, CD24, and ALDH1 for CSCs detection. Data analyses showed significant relationships between MGP and CSCs (P = 0.037), VM and CSCs (P = 0.013), lymph node status and CSCs (P = 0.0001), lymph node status and EMT (P = 0.003), IST and LVI (P = 0.05), VM and LVI (P = 0.01), VM and lymph node status (P = 0.007), and LVI and lymph node status (P = 0.04). Results indicated the prognostic value of MGP, VM, and CSCs with respect to confirmed prognostic markers, including LVI and lymph node involvement, in CMGCs.

scholarly journals Transforming growth factor-β1 inhibits casein secretion from differentiating mammary-gland explants but not from lactating mammary cells

1994 ◽  
Vol 304 (2) ◽  
pp. 333-336 ◽  
Author(s):  
A W Sudlow ◽  
C J Wilde ◽  
R D Burgoyne
Keyword(s):  
Mammary Gland ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β1 ◽  
Mouse Mammary Gland ◽  
Mammary Cells ◽  
Tgf Beta ◽  
Regulatory Effects ◽  
And Function

Transforming growth factor-beta (TGF beta) is important in the maturation and function of the mammary gland and is present in milk. We have examined whether, in addition to inhibiting lactogenesis, TGF beta exerts acute regulatory effects on lactating mammary cells. The isoform TGF beta 1 at 5 and 50 ng/ml suppressed the onset of lactation and the subsequent production of beta-casein by differentiating mouse mammary explants from pregnant mice. By contrast, it did not inhibit protein synthesis or secretion from acini isolated from lactating-mouse mammary gland or protein secretion from explants from lactating mice. These data indicate that TGF beta inhibits the onset of casein secretion, but is not an acute regulator of casein synthesis or secretion from differentiated lactating mammary cells.

Cancer Stem Cells Regulate Cancer-Associated Fibroblasts via Activation of Hedgehog Signaling in Mammary Gland Tumors

2017 ◽  
Vol 77 (8) ◽  
pp. 2134-2147 ◽  
Author(s):  
Giovanni Valenti ◽  
Hazel M. Quinn ◽  
Guus J.J.E. Heynen ◽  
Linxiang Lan ◽  
Jane D. Holland ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Cancer Stem Cells ◽  
Hedgehog Signaling ◽  
Cancer Associated Fibroblasts ◽  
Mammary Gland Tumors

scholarly journals Identification of Stem Cell Units in the Terminal End Bud and Duct of the Mouse Mammary Gland

2001 ◽  
Vol 1 (3) ◽  
pp. 133-143 ◽  
Author(s):  
Nicholas J. Kenney ◽  
Gilbert H. Smith ◽  
Erin Lawrence ◽  
J. Carl Barrett ◽  
David S. Salomon
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mouse Mammary Gland ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells ◽  
Zonula Occludens ◽  
Terminal End Bud ◽  
Transitional Cells

The mouse mammary gland may undergo cycles of proliferation, terminal differentiation, tissue remodeling, and more importantly malignant transformation.Mammary epithelial stem cells and their progeny participate in these processes.Mammary epithelial stem cells are multipotent, exhibit properties of self renewal (up to 7 divisions)and may exist either as long-lived nondividing cells or as proliferating-differentiating cells. The focus of this study was to locate stem cells by identifying them as long-lived, label-retaining mammary epithelial cells (LRCs)in growth active (developing)or growth static (aged)mammar y ducts. Initially, primary epithelial cells were pulse labeled with either fluorescent tracker dye and/or BrdU. Cells were then transplanted into cleared juvenile syngeneic mammary fat pads and held for 5weeks or 8weeks. In this study, we demonstrate that LRCs are stem cells and their progeny (transitional cells)ar e arranged as transitional units (TUs). Additionally, TUs are located every 250 ± 75 µm in ducts or in the terminal end bud 200–600 µm in diameter. Molecules expressed in TUs were Zonula Occludens-1 and α-catenin proteins which were significantly detected in 75%–91% (P < 0.001)of the LRCs cells that make up the TU. These data suggest that transitional units may be a group of label-retaining stem cells and maybe involved in the developmental or cancer process.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

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