Hormonal Regulation of Sodium-Dependent Phosphate Transport in Opossum Kidney Cells

2000 ◽  
Vol 54 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Douglas M. Silverstein ◽  
Adrian Spitzer ◽  
Mario Barac-Nieto
Keyword(s):  
Hormonal Regulation ◽  
Phosphate Transport ◽  
Kidney Cells ◽  
Opossum Kidney ◽  
Opossum Kidney Cells ◽  
Sodium Dependent

Modulation of Na+-Pi cotransport in opossum kidney cells by extracellular phosphate

1988 ◽  
Vol 255 (2) ◽  
pp. C155-C161 ◽  
Author(s):  
J. Biber ◽  
J. Forgo ◽  
H. Murer
Keyword(s):  
Actinomycin D ◽  
Phosphate Transport ◽  
Kidney Cells ◽  
Transport Activity ◽  
Similar Extent ◽  
Opossum Kidney ◽  
Ok Cells ◽  
Opossum Kidney Cells ◽  
Dependent Transport ◽  
Posttranscriptional Level

The effect of the extracellular concentration of Pi on the Na+-dependent phosphate transport activity of OK cells was investigated. When incubated with extracellular Pi at concentrations of 200 microM or less, Na+-Pi cotransport increased approximately twofold in OK cells compared with control cells (kept in 0.85 mM Pi), whereas other Na+-dependent transport activities were not affected. After Pi deprivation, Na+-Pi cotransport could be inhibited to a similar extent (80%) by parathyroid hormone (PTH) as in control cells, suggesting that the PTH-sensitive Na+-Pi cotransport activity is also regulated by extracellular Pi. The increase of Na+-Pi cotransport was maximally expressed after 6 h and could be prevented by cycloheximide (70 microM) but not by actinomycin D (0.5-5 g/ml). However, the adaptive response was completely blocked by 3'-deoxyadenosine (cordycepin) at 100 microM. From these data, it is concluded that the upregulation of Na+-Pi cotransport in OK cells due to low extracellular Pi is controlled at a posttranscriptional level.

scholarly journals Relationship between sodium-dependent phosphate transporter (NaPi-IIc) function and cellular vacuole formation in opossum kidney cells

2015 ◽  
Vol 62 (3.4) ◽  
pp. 209-218 ◽  
Author(s):  
Yuji Shiozaki ◽  
Hiroko Segawa ◽  
Saori Ohnishi ◽  
Akiko Ohi ◽  
Mikiko Ito ◽  
...  
Keyword(s):  
Phosphate Transporter ◽  
Kidney Cells ◽  
Vacuole Formation ◽  
Opossum Kidney ◽  
Opossum Kidney Cells ◽  
Sodium Dependent
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