Studies on the Estimation of Beta-Alpha Lipoprotein Ratio in Human Serum by Paper Electrophoresis

Pharmacology ◽  
1959 ◽  
Vol 1 (5) ◽  
pp. 281-285
Author(s):  
Manju Mukherjea ◽  
B.P. Ghosh
Keyword(s):  
Human Serum ◽  
Paper Electrophoresis

Cholesterol in Serum and Lipoprotein Fractions

1956 ◽  
Vol 2 (3) ◽  
pp. 145-159 ◽  
Author(s):  
Joseph T Anderson ◽  
Ancel Keys
Keyword(s):  
Human Serum ◽  
Total Cholesterol ◽  
Filter Paper ◽  
Room Temperature ◽  
Paper Electrophoresis ◽  
Cholesterol Content ◽  
Intraindividual Variability ◽  
Detailed Data ◽  
The Stability ◽  
Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

Measurement of Polarographically Active Sulfur in Human Serum Fractions Obtained by Paper Electrophoresis

1970 ◽  
Vol 16 (3) ◽  
pp. 155-157 ◽  
Author(s):  
Jiří Homolka
Keyword(s):  
Human Serum ◽  
Serum Protein ◽  
Paper Electrophoresis ◽  
Protein Fractions ◽  
X Ray ◽  
Serum Fractions

Abstract Polarographically active sulfur was distributed among the serum-protein fractions as follows (in percentages): albumin, 63.21; α1-globulin, 3.17; α2-globulin, 7.92; β-globulin, 14.54; α-globulin, 11.16. The results compare well with those obtained by X-ray spectrometry. The same method was applied to sera from patients suffering from malignant processes and some other diseases; the percentage of polarographically active sulfur in fractions of plasma from diseased individuals varied considerably.

Immunoglobulin Components of "Gamma" Globulin in Human Serum

1965 ◽  
Vol 11 (4) ◽  
pp. 527-530 ◽  
Author(s):  
Virginia E Trevorrow ◽  
Deborah Merrill ◽  
Henry N Claman
Keyword(s):  
Human Serum ◽  
Gamma Globulin ◽  
Paper Electrophoresis ◽  
Agar Gel ◽  
M Proteins ◽  
Human Sera

Abstract Total gamma globulin was measured after paper electrophoresis of 40 human sera. Gamma G, γ A, and γ M immunoproteins were measured in these sera by single diffusion in agar gel. The total gamma globulin was found to consist primarily of γ G and γ M proteins, with a small portion of the γ A also included.

scholarly journals ZONE PAPER ELECTROPHORESIS STUDIES ON RADIO-IODINATED HUMAN SERUM ALBUMIN 1

1954 ◽  
Vol 33 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Elemer R. Gabrieli ◽  
Dicran Goulian ◽  
Thorn Kinersly ◽  
Raymond Collet
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Paper Electrophoresis
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