Demonstration by Light Microscopy of Cytomegalovirus on a Renal Biopsy of a Renal Allograft Recipient: Confirmation by Immunohistochemistry and in situ Hybridization

An anaerobic migrating blanket reactor (AMBR) was seeded with flocculent biomass from a digester and fed a substrate consisting of volatile fatty acids and sucrose to study granulation. After three months of operation, a mature granular blanket developed in the reactor. Moreover, fibers of approximately 1 cm long had become prevalent in the AMBR. Scanning electron microscopy (SEM) and light microscopy revealed a very dense structure consisting of bundles of filaments resembling Methanosaeta cells. Further studies with fluorescence in-situ hybridization (FISH), showed that Methanosaeta concilii was the predominant microorganism in these fibers.

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Detection of human papillomavirus in skin and genital lesions of renal allograft recipients by in situ hybridization

Histopathology ◽

10.1111/j.1365-2559.1990.tb01087.x ◽

2007 ◽

Vol 16 (2) ◽

pp. 181-185 ◽

Cited By ~ 11

Author(s):

K. BLESSING ◽

K.K. McLAREN ◽

R. MORRIS ◽

B.B.B. BARR ◽

E.E. BENTON ◽

...

Keyword(s):

In Situ Hybridization ◽

Human Papillomavirus ◽

Renal Allograft

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In situ Hybridization in Light Microscopy

Journal of Microscopy ◽

10.1046/j.1365-2818.2003.11411.x ◽

2003 ◽

Vol 210 (2) ◽

pp. 187-188

Author(s):

Ton Raap

Keyword(s):

In Situ Hybridization ◽

Light Microscopy

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Preparing plant chromosomes for scanning electron microscopy

Genome ◽

10.1139/g90-052 ◽

1990 ◽

Vol 33 (3) ◽

pp. 333-339 ◽

Cited By ~ 17

Author(s):

John E. Dillé ◽

Douglas C. Bittel ◽

Kathleen Ross ◽

J. Perry Gustafson

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

In Situ Hybridization ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Light Microscopy ◽

Plant Chromosomes ◽

Cellular Debris ◽

Scanning Electron

The scanning electron microscope may be useful in the analysis of plant chromosomes treated with in situ hybridization, especially when the probes and (or) chromosomes are near or beyond the resolution of the light microscope. Usual methods of plant chromosome preparation are unsuitable for scanning electron microscope observation as a result of cellular debris, which also interferes with probe hybridization. A method is described whereby protoplasts are obtained from fixed root tips by enzymatic digestion and applied to slides in a manner that produces little or no cellular debris overlying the chromosomes. The slides were examined by scanning electron microscopy and light microscopy after C-banding and in situ hybridization with a rye nucleolus organizer region spacer probe. This technique, which allows for scanning electron microscope visualization of bands and probes not easily identified with light microscopy, should prove useful in the physical mapping of low copy number or unique DNA sequences.Key words: protoplasts, rice, wheat, rye, physical maps, in situ hybridization.

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