Skin Microdialysis: Detection of in vivo Histamine Release in Cutaneous Allergic Reactions

Analysis of literature data presented in search engines — Elibrary, PubMed, Cochrane — concerning the risk of developing type I allergic reactions in patients with blood diseases is presented. It is shown that the most common cause of type I allergic reactions is drugs included in the treatment regimens of this category of patients. The article presents statistics on the increase in the number of drug allergies leading to cases of anaphylactic shock in patients with blood diseases. Modern methods for the diagnosis of type I allergic reactions in vivo and in vitro are considered.

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Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo

Journal of Experimental Medicine ◽

10.1084/jem.20042085 ◽

2005 ◽

Vol 201 (3) ◽

pp. 385-396 ◽

Cited By ~ 47

Author(s):

Stefan Kraft ◽

Tony Fleming ◽

James M. Billingsley ◽

Shih-Yao Lin ◽

Marie-Hélène Jouvin ◽

...

Keyword(s):

Pi3k Pathway ◽

Therapeutic Agents ◽

Allergic Reactions ◽

Ecm Proteins ◽

Broad Array ◽

High Affinity Ige Receptor ◽

Nonadherent Cells ◽

Tetraspanin Cd63

High-affinity IgE receptor (FcεRI) cross-linking on mast cells (MCs) induces secretion of preformed allergy mediators (degranulation) and synthesis of lipid mediators and cytokines. Degranulation produces many symptoms of immediate-type allergic reactions and is modulated by adhesion to surfaces coated with specific extracellular matrix (ECM) proteins. The signals involved in this modulation are mostly unknown and their contribution to allergic reactions in vivo is unclear. Here we report the generation of monoclonal antibodies that potently suppress FcεRI-induced degranulation, but not leukotriene synthesis. We identified the antibody target as the tetraspanin CD63. Tetraspanins are membrane molecules that form multimolecular complexes with a broad array of molecules including ECM protein-binding β integrins. We found that anti-CD63 inhibits MC adhesion to fibronectin and vitronectin. Furthermore, anti-CD63 inhibits FcεRI-mediated degranulation in cells adherent to those ECM proteins but not in nonadherent cells. Thus the inhibition of degranulation by anti-CD63 correlates with its effect on adhesion. In support of a mechanistic linkage between the two types of inhibition, anti-CD63 had no effect on FcεRI-induced global tyrosine phosphorylation and calcium mobilization but impaired the Gab2–PI3K pathway that is known to be essential for both degranulation and adhesion. Finally, we showed that these antibodies inhibited FcεRI-mediated allergic reactions in vivo. These properties raise the possibility that anti-CD63 could be used as therapeutic agents in MC-dependent diseases.

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A Novel Paclitaxel Microemulsion Containing a Reduced Amount of Cremophor EL: Pharmacokinetics, Biodistribution, andIn VivoAntitumor Efficacy and Safety

Journal of Biomedicine and Biotechnology ◽

10.1155/2011/854872 ◽

2011 ◽

Vol 2011 ◽

pp. 1-10 ◽

Cited By ~ 15

Author(s):

Ying Wang ◽

Ke-Chun Wu ◽

Bing-Xiang Zhao ◽

Xin Zhao ◽

Xin Wang ◽

...

Keyword(s):

Antitumor Activity ◽

Therapeutic Efficacy ◽

Antitumor Efficacy ◽

Allergic Reactions ◽

Cremophor El ◽

Efficacy And Safety ◽

Tumor Bearing ◽

Allergic Effect ◽

Injection Product

The purpose of this study was to prepare a novel paclitaxel (PTX) microemulsion containing a reduced amount of Cremophor EL (CrEL) which had similar pharmacokinetics and antitumor efficacy as the commercially available PTX injection, but a significantly reduced allergic effect due to the CrEL. The pharmacokinetics, biodistribution,in vivoantitumor activity and safety of PTX microemulsion was evaluated. The results of pharmacokinetic and distribution properties of PTX in the microemulsion were similar to those of the PTX injection. The antitumor efficacy of the PTX microemulsion in OVCRA-3 and A 549 tumor-bearing animals was similar to that of PTX injection. The PTX microemulsion did not cause haemolysis, erythrocyte agglutination or simulative reaction. The incidence and degree of allergic reactions exhibited by the PTX microemulsion group, with or without premedication, were significantly lower than those in the PTX injection group (P<.01). In conclusion, the PTX microemulsion had similar pharmacokinetics and anti-tumor efficacy to the PTX injection, but a significantly reduced allergic effect due to CrEL, indicating that the PTX microemulsion overcomes the disadvantages of the conventional PTX injection and is one way of avoiding the limitations of current injection product while providing suitable therapeutic efficacy.

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Mast cell tumor histamine release following morphine exposure both in vitro and in vivo

Veterinary Anaesthesia and Analgesia ◽

10.1016/j.vaa.2018.09.012 ◽

2018 ◽

Vol 45 (6) ◽

pp. 885.e4

Author(s):

Taylor Curley ◽

Pedro Boscan ◽

Douglas Thamm ◽

Sam Johnson

Keyword(s):

Mast Cell ◽

Histamine Release ◽

Cell Tumor ◽

Mast Cell Tumor

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Morphine Alteration of Histamine Release in vivo

The Brain Immune Axis and Substance Abuse - Advances in Experimental Medicine and Biology ◽

10.1007/978-1-4615-1951-5_22 ◽

1995 ◽

pp. 161-168 ◽

Cited By ~ 5

Author(s):

Jack M. Risdahl ◽

Michael J. Huether ◽

Kristen V. Gustafson ◽

Thomas W. Molitor

Keyword(s):

Histamine Release

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MiR-154-5p-MCP1 Axis Regulates Allergic Inflammation by Mediating Cellular Interactions

Frontiers in Immunology ◽

10.3389/fimmu.2021.663726 ◽

2021 ◽

Vol 12 ◽

Author(s):

Misun Kim ◽

Hyein Jo ◽

Yoojung Kwon ◽

Myeong Seon Jeong ◽

Hyun Suk Jung ◽

...

Keyword(s):

Allergic Inflammation ◽

Passive Cutaneous Anaphylaxis ◽

Allergic Reactions ◽

Dependent Manner ◽

Cellular Interactions ◽

Array Analysis ◽

Molecular Features ◽

Rat Basophilic Leukemia Cells

In a previous study, we have demonstrated that p62, a selective receptor of autophagy, can regulate allergic inflammation. In the present study, microRNA array analysis showed that miR-154-5p was increased by antigen (DNP-HSA) in a p62-dependent manner in rat basophilic leukemia cells (RBL2H3). NF-kB directly increased the expression of miR-154-5p. miR-154-5p mediated in vivo allergic reactions, including passive cutaneous anaphylaxis and passive systemic anaphylaxis. Cytokine array analysis showed that antigen stimulation increased the expression of MCP1 in RBL2H3 cells in an miR-154-5p-dependent manner. Reactive oxygen species (ROS)-ERK-NF-kB signaling increased the expression of MCP1 in antigen-stimulated RBL2H3 cells. Recombinant MCP1 protein induced molecular features of allergic reactions both in vitro and in vivo. Anaphylaxis-promoted tumorigenic potential has been known to be accompanied by cellular interactions involving mast cells, and macrophages, and cancer cells. Our experiments employing culture medium, co-cultures, and recombinant MCP1 protein showed that miR-154 and MCP1 mediated these cellular interactions. MiR-154-5p and MCP1 were found to be present in exosomes of RBL2H3 cells. Exosomes from PSA-activated BALB/C mouse induced molecular features of passive cutaneous anaphylaxis in an miR-154-5p-dependent manner. Exosomes from antigen-stimulated RBL2H3 cells enhanced both tumorigenic and metastatic potentials of B16F1 melanoma cells in an miR-154-5p-dependent manner. Exosomes regulated both ROS level and ROS mediated cellular interactions during allergic inflammation. Our results indicate that the miR-154-5p-MCP1 axis might serve as a valuable target for the development of anti-allergy therapeutics.

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