Fel d 1-Specific IgG Antibodies Induced by Natural Exposure Have Blocking Activity in Skin Tests

1996 ◽  
Vol 109 (4) ◽  
pp. 369-375 ◽  
Author(s):  
A.M. Witteman ◽  
S.O. Stapel ◽  
D.H.S. Sjamsoedin ◽  
H.M. Jansen ◽  
R.C. Aalberse ◽  
...  
Keyword(s):  
Skin Tests ◽  
Igg Antibodies ◽  
Natural Exposure ◽  
Fel D 1 ◽  
Blocking Activity ◽  
Specific Igg ◽  
Specific Igg Antibodies

Blocking activity of mite-specific IgG antibodies studied by skin tests

Allergy ◽  
1989 ◽  
Vol 44 (6) ◽  
pp. 427-431 ◽  
Author(s):  
G. PECQUET ◽  
M. MURRIETA ◽  
V. MICHELEN ◽  
F. LEYNADIER ◽  
J. DRY ◽  
...  
Keyword(s):  
Skin Tests ◽  
Igg Antibodies ◽  
Blocking Activity ◽  
Specific Igg ◽  
Specific Igg Antibodies

scholarly journals Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition ofDermatophagoides pteronyssinusAntigens: AnIn VitroStudy

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Isabella Lima Siman ◽  
Lais Martins de Aquino ◽  
Leandro Hideki Ynoue ◽  
Juliana Silva Miranda ◽  
Ana Claudia Arantes Marquez Pajuaba ◽  
...  
Keyword(s):  
Ammonium Sulfate ◽  
Dermatophagoides Pteronyssinus ◽  
Igg Antibodies ◽  
Humoral Immune ◽  
Ige Reactivity ◽  
Sds Page ◽  
Blocking Activity ◽  
Specific Igg ◽  
Specific Igg Antibodies

One of the purposes of specific immunotherapy (SIT) is to modulate humoral immune response against allergens with significant increases in allergen-specific IgG levels, commonly associated with blocking activity. The present study investigatedin vitroblocking activity of allergen-specific IgG antibodies on IgE reactivity toDermatophagoides pteronyssinus(Dpt) in sera from atopic patients. Dpt-specific IgG antibodies were purified by ammonium sulfate precipitation followed by protein-G affinity chromatography. Purity was checked by SDS-PAGE and immunoreactivity by slot-blot and immunoblot assays. The blocking activity was evaluated by inhibition ELISA. The electrophoretic profile of the ammonium sulfate precipitated fraction showed strongly stained bands in ligand fraction after chromatography, compatible with molecular weight of human whole IgG molecule. The purity degree was confirmed by detecting strong immunoreactivity to IgG, negligible to IgA, and no reactivity to IgE and IgM. Dpt-specific IgG fraction was capable of significantly reducing levels of IgE anti-Dpt, resulting in 35%–51% inhibition of IgE reactivity to Dpt in atopic patients sera. This study showed that allergen-specific IgG antibodies purified from mite-allergic patients sera block the IgE recognition ofDermatophagoides pteronyssinusantigens. This approach reinforces that intermittent measurement of serum allergen-specific IgG antibodies will be an important objective laboratorial parameter that will help specialists to follow their patients under SIT.

scholarly journals Endogenously Produced SARS-CoV-2 Specific IgG Antibodies May Have a Limited Impact on Clearing Nasal Shedding of Virus during Primary Infection in Humans

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 516
Author(s):  
Shuyi Yang ◽  
Keith R. Jerome ◽  
Alexander L. Greninger ◽  
Joshua T. Schiffer ◽  
Ashish Goyal
Keyword(s):  
Production Rate ◽  
Neutralizing Antibodies ◽  
Primary Infection ◽  
Natural Infection ◽  
Viral Clearance ◽  
Clinical Severity ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
Specific Igg ◽  
Specific Igg Antibodies

While SARS-CoV-2 specific neutralizing antibodies have been developed for therapeutic purposes, the specific viral triggers that drive the generation of SARS-CoV-2 specific IgG and IgM antibodies remain only partially characterized. Moreover, it is unknown whether endogenously derived antibodies drive viral clearance that might result in mitigation of clinical severity during natural infection. We developed a series of non-linear mathematical models to investigate whether SARS-CoV-2 viral and antibody kinetics are coupled or governed by separate processes. Patients with severe disease had a higher production rate of IgG but not IgM antibodies. Maximal levels of both isotypes were governed by their production rate rather than different saturation levels between people. Our results suggest that an exponential surge in IgG levels occurs approximately 5–10 days after symptom onset with no requirement for continual antigenic stimulation. SARS-CoV-2 specific IgG antibodies appear to have limited to no effect on viral dynamics but may enhance viral clearance late during primary infection resulting from the binding effect of antibody to virus, rather than neutralization. In conclusion, SARS-CoV-2 specific IgG antibodies may play only a limited role in clearing infection from the nasal passages despite providing long-term immunity against infection following vaccination or prior infection.

P161 Kidney allograft outcomes associated with locus specific IgG antibodies to donor class I

2017 ◽  
Vol 78 ◽  
pp. 175
Author(s):  
Rex Friedlander ◽  
Essa Abuhelaiqa ◽  
Prabhakar Putheti ◽  
Arvind K. Menon ◽  
Vijay K. Sharma ◽  
...  
Keyword(s):  
Class I ◽  
Igg Antibodies ◽  
Kidney Allograft ◽  
Specific Igg ◽  
Specific Igg Antibodies

scholarly journals Total serum IgE and parasite: specific IgG and IgA antibodies in human strongyloidiasis

1993 ◽  
Vol 35 (4) ◽  
pp. 361-365 ◽  
Author(s):  
Cláudio L. Rossi ◽  
Emilia E. H. Takahashi ◽  
Cláudia D. Partel ◽  
Lívia G.V.L. Teodoro ◽  
Luiz J. da Silva
Keyword(s):  
Elevated Serum ◽  
Clinical Manifestations ◽  
Case Series ◽  
Total Serum ◽  
Igg Antibodies ◽  
Serum Ige ◽  
Iga Antibodies ◽  
Specific Igg ◽  
Mean Concentration ◽  
Specific Igg Antibodies

Total serum IgE, and Strongyloides - specific IgG and IgA antibodies were studied in 27 patients with parasitologically proven strongyloidiasis. Clinical manifestations in this case series were investigated by a restrospective study of the patient's records. Total serum IgE levels were elevated (greater than 250 IU/ml) in 59% of the patients (mean concentration = 1364 IU/ml). Parasite - specific IgG and IgA antibodies were detected by ELISA in the serum of 23 (85.2%) and 21 (77.8%) patients, respectively. Elevated serum IgE and clinical manifestations were not useful indexes of the presence of strongyloidiasis. On the other hand, our results support the view that serologic tests, particularly ELISA for detecting Strongyloides - specific IgG antibodies, can be usefully exploited for diagnostic purposes in strongyloidiasis.

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