Biocompatibility of Various Dialysis Membranes as Assessed by Coagulation Assay

SummaryHuman umbilical vein endothelial cells in culture synthesize thromboplastin upon stimulation with phytohaemagglutinin (PHA) or the tumor promotor 12-O-tetradecanoyl-phorbol-13-acetate (TPA). The thromboplastin activity is further strongly enhanced in a time dependent reaction by the presence of gel-filtered platelets or platelet aggregates. This effect was demonstrable at platelet concentrations lower than those normally found in plasma, it may thus be of pathophysiological relevance. The thromboplastin activity increased with increasing number of platelets added. Cycloheximide inhibited the increase, suggesting that de novo synthesis of the protein component of thromboplastin, apoprotein III, is necessary.When care was taken to remove monocytes no thromboplastin activity and no apoprotein HI antigen could be demonstrated in suspensions of gel-filtered platelets, platelets aggregated with thrombin or homogenized platelets when studied with a coagulation assay and an antibody neutralization technique.

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Rotational thrombelastometry (ROTEM) improves hemostasis assessment compared to conventional coagulation test in ACLF and Non-ACLF patients

BMC Gastroenterology ◽

10.1186/s12876-020-01413-w ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Jessica Seeßle ◽

Jan Löhr ◽

Marietta Kirchner ◽

Josefin Michaelis ◽

Uta Merle

Keyword(s):

Liver Cirrhosis ◽

Test Performance ◽

Coagulation System ◽

Bleeding Events ◽

Coagulation Parameters ◽

Normal Limits ◽

Coagulation Tests ◽

Prospective Assessment ◽

Patient Groups ◽

Coagulation Assay

Abstract Background Patients with liver cirrhosis typically exhibit abnormal coagulation parameters in conventional coagulation tests (CCTs). Rotational thromboelastometry (ROTEM) is a holistic blood coagulation assay. This method provides an insight into the global hemostatic capabilities and has been suggested to provide a better overview of the coagulation system in liver cirrhosis. Methods The goal of this study was to examine hemostasis in patients with stable liver cirrhosis (Non-ACLF) and in acute-on-chronic liver failure (ACLF) by CCT and ROTEM including agreement of both tests and the prospective assessment of test performance based on clinical outcomes in ACLF patients. Therefore, ACLF patients were additionally subgrouped by bleeding events. Fifty-five Non-ACLF patients and twenty-two patients with ACLF were analysed in this prospective cohort study. Results Coagulation parameters analysed by CCT were outside the normal range in Non-ACLF and ACLF patients, but were significantly more aberrant in ACLF patients. Non-ACLF patients analysed by ROTEM revealed parameters largely within the normal limits, while significantly more ROTEM parameters in ACLF patients were affected. Maximum clot firmness (MCF) was significantly divergent between both patient groups and correlated well with levels of fibrinogen and platelet count. Using Cohen’s Kappa coefficient κ, the strength of agreement between CCT and ROTEM analyses was determined to be fair for Non-ACLF patients and moderate for ACLF patients. Bleeding events occurred significantly more often in ACLF group with significantly reduced A10 and MCF. Conclusions For assessing hemostasis in Non-ACLF and ACLF patients the underlying dataset shows advantages of ROTEM over CCT. A10 and MCF represent suitable prognostic parameters in predicting bleeding events in ACLF group.

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Blood Components Deposited on Used and Reused Dialysis Membranes

Biomaterials Medical Devices and Artificial Organs ◽

10.3109/10731197609118658 ◽

1976 ◽

Vol 4 (3-4) ◽

pp. 333-358 ◽

Cited By ~ 10

Author(s):

R. G. Mason ◽

W. H. Zucker ◽

R. T. Bilinsky ◽

B. A. Shinoda ◽

D. E. Sharp ◽

...

Keyword(s):

Blood Components ◽

Dialysis Membranes

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Effect of Vitamin E-Coated Dialysis Membranes on Anemia in Patients with Chronic Kidney Disease: An Italian Multicenter Study

The International Journal of Artificial Organs ◽

10.1177/039139880803100610 ◽

2008 ◽

Vol 31 (6) ◽

pp. 545-552 ◽

Cited By ~ 22

Author(s):

D.N. Cruz ◽

M. De Cal ◽

F. Garzotto ◽

A. Brendolan ◽

F. Nalesso ◽

...

Keyword(s):

Chronic Kidney Disease ◽

Vitamin E ◽

Kidney Disease ◽

Multicenter Study ◽

Dialysis Membranes

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Radiocontrast Removal by Dialysis Membranes

Blood Purification ◽

10.1159/000170016 ◽

1991 ◽

Vol 9 (4) ◽

pp. 182-187 ◽

Cited By ~ 9

Author(s):

Steven F. Gouge ◽

Jack Moore Jr. ◽

Frank Atkins ◽

Przemyslaw Hirszel ◽

Michael J. Cory

Keyword(s):

Dialysis Membranes

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Ice-active substances associated with Antarctic freshwater and terrestrial photosynthetic organisms

Antarctic Science ◽

10.1017/s0954102000000493 ◽

2000 ◽

Vol 12 (4) ◽

pp. 418-424 ◽

Cited By ~ 21

Author(s):

James A. Raymond ◽

Christian H. Fritsen

Keyword(s):

Molecular Weight ◽

North America ◽

Ice Crystals ◽

Dry Valleys ◽

Cyanobacterial Mats ◽

Cold Environments ◽

Photosynthetic Organisms ◽

Eukaryotic Algae ◽

Dialysis Membranes ◽

Active Substances

Macromolecular substances that cause pitting and other modifications of growing ice crystals were found to be associated with cyanobacterial mats, eukaryotic algae and mosses from Ross Island and the McMurdo Dry Valleys, Antarctica. Ice-pitting activities were largely retained by dialysis membranes with molecular weight cut-offs of up to 300 kDa. Unlike most aqueous solutes, the ice-active molecules were not excluded from the ice phase during freezing. The ice-pitting activities of each of the samples tested was destroyed by exposure to temperatures between 45 and 65°C, suggesting that they have a protein component. Ice-active substances were not found in cyanobacteria or mosses from temperate climates, but ice-activity was found to be associated with mosses from cold habitats in North America. Although the function of the ice-active substances is not known, their apparent confinement to cold environments suggests that they have a cryoprotective role.

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