Pathologic Study of Supernumerary Orbital Band in Type I Duane Syndrome

Background/Aims: Accessory orbital bands are relatively rare and very few reports detail histopathology. Cases in the literature describe the composition of the bands as muscular and/or fibrous. The composition of the supernumerary band lying deep in the medial rectus muscle in a patient with type I Duane syndrome was investigated. Methods: Histochemical stains were used in conjunction with polarized light for differentiating compressed collagen from muscle. Immunohistochemistry was used for verification of the presence of muscle. Results: Compressed collagen appeared red using Masson trichrome staining. Collagen was positively identified by illumination with polarized light on several stains including the underutilized Sirius red dye. Conclusions: The findings of dense collagen fibers in the fibrotic band with focal striated muscle correlated with the restrictive strabismus. In concert with other cases in the literature, it is proposed that the fibrous bands are generally associated with restrictive strabismus. Bands that are muscular may or may not be associated with strabismus. Special techniques are needed to positively identify compressed collagen.

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A Polarized Light Microscopy Study in a Case of Morphea

ARS Medica Tomitana ◽

10.2478/arsm-2020-0001 ◽

2020 ◽

Vol 26 (1) ◽

pp. 1-4

Author(s):

Mocanu Liliana ◽

Deacu Sorin ◽

Roman Polimaru ◽

Aschie Mariana

Keyword(s):

Light Microscopy ◽

Polarized Light ◽

Microscopy Study ◽

Polarized Light Microscopy ◽

Type I ◽

Elastic Tissue ◽

Collagen Fibres ◽

Reticular Dermis ◽

Plaque Type ◽

Sirius Red

Abstract We report a case of plaque type of scleroderma with specific clinical features and conventional histopathology, with sclerosis and hipocellularity of fibroblasts and preservation of elastic tissue. We describe polarized light microscopy findings, on conventional stained slides and on picro sirius red stained slides. We appreciate that picro sirius red stain allows a better characterization of collagen fibres composition in papillary and reticular dermis, that is severely disturbed in morphea, with an inverse distribution of collagen fibres type I and III comparative with normal dermis.

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Interleukin-6 (IL-6) influence on colonic anastomosis healing in rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502007000200006 ◽

2007 ◽

Vol 22 (2) ◽

pp. 110-114 ◽

Cited By ~ 8

Author(s):

Fernando Hintz Greca ◽

Zacarias Alves de Souza Filho ◽

Allan Giovanini ◽

Carlos Agemiro de Camargo Junior ◽

Michel Risnic Rubin ◽

...

Keyword(s):

Interleukin 6 ◽

Colonic Anastomosis ◽

Collagen Type I ◽

Histological Evaluation ◽

Control Group ◽

Type I ◽

Colonic Healing ◽

Red Dye ◽

Experimental Group ◽

Sirius Red

PURPOSE:To observe the effect of pro-inflamatory citokin, Interleukin-6 (IL-6), administration on the healing of colonic anastomosis in rats. METHODS: Thirty Wistar rats were divided into 2 groups of 15 animals each: a control group and an experiment group. The animals of the experiment group were submitted to injection of 500µg/kg of IL-6 Recombinant (Sandoz Phamarceutical®) subcutaneously 24 and 12 hours preceding the surgery, and the control group, were submitted to the injection of saline solution, using the same volume of solution as well as the way of administration. The rats were submitted to an end to end colonic anastomosis, being sacrificed in the 7th post-operative day, the site of the anastomosis was evaluated regarding to the adhesions that was quantified and the presence of complications was observed. The colon containing the anastomosis was submitted to a tensiometric study and a sample were sent for histological studies with HE and Sirius Red dye. RESULTS: The macroscopic and the tensiometric study did not demonstrate any significant differences between the control and experiment group. Regarding the histological evaluation, there was a significant reduction of the chronic inflammatory process in the experiment group (p=0,0209) and a lesser granulation area (p=0,0099). The total amount of collagen was similar in both groups, however, the experimental group showed a predominant area of immature collagen (p=0.009). CONCLUSIONS: The longer persistence of the acute phase of wound healing in the experiment group, as well as a decreased of the mature collagen type I, demonstrating that the IL-6 was harmful to the colonic healing anastomosis in rats.

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RESULTS OF POLARIZED LIGHT USE FOR STUDY OF STRESS-STRAIN STATE OF CORNEA IN PATHOLOGY OF EXTRAOCULAR MUSCLES

International Medical Journal ◽

10.37436/2308-5274-2019-3-12 ◽

2020 ◽

pp. 65-69

Author(s):

Nataliia Mykhailivna Kovtun ◽

Ihor Volodymyrovych Kaplin

Keyword(s):

Interference Pattern ◽

Optical Anisotropy ◽

Extraocular Muscle ◽

Strain State ◽

Polarized Light ◽

Attachment Site ◽

Rectus Muscle ◽

Extraocular Muscles ◽

Asymmetric Distortion ◽

Interference Picture

The parameters of interference patterns observed on the cornea in a polarized light in pathology of extraocular muscles were quantitatively evaluated. A study of the shape of 147 interference patterns observed on the cornea in a polarized light showed that the horizontal deviation of an eye in 56 % of cases was caused by the displacement of the attachment of the upper rectus muscle towards the inner rectus muscle. During illumination of the cornea of a live eye with polarized light, a specific interference pattern in the form of a rhombus formed by rainbow stripes (isochromes) is observed. It is established that such an interference pattern is the result of the influence of extraocular muscles on the cornea. The findings showed that the weakening of the force of one of muscles leads to asymmetric distortion of the shape of the interference rhombus, which is manifested with a reduced length of the corresponding section of the diagonal. On the contrary, as the force of the muscle increases, the corresponding section of the diagonal enhances. The displacement of the attachment site of direct extraocular muscle along the line of action is equivalent to a change in effort on its part, indicated appropriately by the shape of the interference rhombus. As the muscle moves away from the line of action, the angle of the interference rhombus is shifted away from the corresponding meridian. Key words: polarized light, optical anisotropy, cornea, extra−ocular muscles, interference picture.

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Results of bilateral medial rectus muscle recession in unilateral esotropic Duane syndrome

Journal of American Association for Pediatric Ophthalmology and Strabismus ◽

10.1016/j.jaapos.2009.10.012 ◽

2010 ◽

Vol 14 (1) ◽

pp. 103 ◽

Cited By ~ 2

Author(s):

Marc F. Greenberg

Keyword(s):

Rectus Muscle ◽

Medial Rectus ◽

Duane Syndrome

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Histotopography of haemorrhagic infiltration in the hanging cutaneous furrow: Where to look for haemorrhagic infiltration in hanging

Medicine Science and the Law ◽

10.1177/00258024211023246 ◽

2021 ◽

pp. 002580242110232

Author(s):

Guendalina Gentile ◽

Stefano Tambuzzi ◽

Salvatore Andreola ◽

Riccardo Zoja

Keyword(s):

Adipose Tissue ◽

Microscopic Examination ◽

Subcutaneous Adipose Tissue ◽

Transition Point ◽

Striated Muscle ◽

Forensic Evidence ◽

Hematoxylin And Eosin ◽

Compression Area ◽

Subcutaneous Adipose ◽

Masson Trichrome Staining

The forensic evidence of hanging is based on the autopsy demonstration of the traces left by the noose or the ligature on the neck, as well as on the histological assessment of the hanging mark vitality. However, the specific topography of haemorrhagic infiltration in the context of the cervical damaged tissues involved in hanging is not known. We carried out an extensive microscopic examination to identify if haemorrhagic infiltration in hanging appeared in specific topographic locations that could have been considered as elective. From 102 victims of suicidal hanging, a skin fragment was sampled from the maximum compression area of the sulcus, including the skin portions immediately above and below it. The sampling was also extended to collect the subcutaneous adipose tissue and part of the striated muscle. A standard histological examination was performed on all the samples, and hematoxylin and Eosin, Weigert’s resorcin-fuchsin and Goldner’s Masson trichrome staining were performed. In all the cases assessed, the microscopic examination allowed the detection of haemorrhagic infiltration mainly in the deepest areas under the sulcus and especially in the deep dermis and at the transition point between the dermis and the subcutaneous adipose tissue, as well as in the context of its supporting connective tissue. Such areas could therefore be considered as regions in which the presence of haemorrhagic infiltration is more likely to be demonstrated. Accordingly, we recommend performing sampling similar to ours and focusing the search for haemorrhagic infiltration as suggested.

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Vertical rectus muscle augmented transposition in Duane syndrome

Journal of American Association for Pediatric Ophthalmology and Strabismus ◽

10.1067/mpa.2001.112677 ◽

2001 ◽

Vol 5 (2) ◽

pp. 105-113 ◽

Cited By ~ 40

Author(s):

Federico G. Velez ◽

R.Scott Foster ◽

Arthur L. Rosenbaum

Keyword(s):

Rectus Muscle ◽

Duane Syndrome

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Contralateral medial rectus muscle recession in patients with Duane syndrome

Journal of American Association for Pediatric Ophthalmology and Strabismus ◽

10.1016/j.jaapos.2004.01.005 ◽

2004 ◽

Vol 8 (3) ◽

pp. 296 ◽

Cited By ~ 5

Author(s):

Steven M Archer

Keyword(s):

Rectus Muscle ◽

Medial Rectus ◽

Duane Syndrome

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Polarized Light Microscopy for Analyzing Tissue Mechanics During In Vitro Acupuncture

ASME 2008 Summer Bioengineering Conference, Parts A and B ◽

10.1115/sbc2008-192595 ◽

2008 ◽

Author(s):

Lowell Taylor Edgar ◽

Margaret Julias ◽

David I. Shreiber ◽

Helen M. Buettner

Keyword(s):

Connective Tissue ◽

Light Microscopy ◽

Type I Collagen ◽

Polarized Light ◽

Tissue Mechanics ◽

Polarized Light Microscopy ◽

Tissue Response ◽

Type I ◽

Collagen Gels

Acupuncture is a traditional therapy originating in China almost 2000 years ago. Acupuncture has slowly been growing in popularity in the West, and clinical evidence has shown the potential for acupuncture as a low-cost ‘alternative’ therapy for an assortment of ailments [1]. The practice of acupuncture involves inserting fine needles into the skin followed by needle manipulation, usually by rotation. Recent studies by Langevin et al demonstrate that this rotation causes the subcutaneous connective tissue to couple to and wind around the needle [2–4], which suggests that mechanotransduction in the connective tissue might play a role in the therapeutic mechanisms that underlay acupuncture [2, 3]. To begin to decompose and quantify this complex mechanism at the tissue level in a controlled setting, we have simulated acupuncture in type I collagen gels in vitro, and have developed algorithms to quantify the tissue response following imaging with polarized light microscopy (PLM).

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