scholarly journals Pathological Analysis of Early Transplant Glomerulopathy in Renal Allografts Using Low-Vacuum Scanning Electron Microscopy

Nephron ◽  
2020 ◽  
pp. 1-8
Author(s):  
Hiroka Onishi ◽  
Hideyo Oguchi ◽  
Kazunobu Shinoda ◽  
Tetuo Mikami ◽  
Taichi Arai ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Capillary Number ◽  
Periodic Acid ◽  
Kidney Transplant Recipients ◽  
Average Percentage ◽  
Antibody Mediated Rejection ◽  
Biopsy Specimens ◽  
Transplant Glomerulopathy ◽  
Scanning Electron

<b><i>Aim:</i></b> Low-vacuum scanning electron microscopy (LVSEM) has been reported to aid in diagnosis of renal biopsy. This study evaluated early transplant glomerulopathy in kidney transplant recipients using LVSEM. <b><i>Methods:</i></b> We selected 4 biopsies of cg0, 5 biopsies of cg1a, 5 biopsies of cg1b, and 4 biopsies of cg2 lesions that had been evaluated by light microscopy (LM) and transmission electron microscopy from recipients with acute/active or chronic, active antibody-mediated rejection (AABMR or CAABMR). Renal allograft paraffin sections (1 µm thickness) were stained with periodic acid-methenamine silver and observed using LVSEM. The cg score was based on the Banff classification. The parameter “percentage of duplicated capillary number” was calculated as follows: in 1 glomerulus with glomerular basement membrane (GBM) duplication, the total duplicated capillary number/the total number of capillaries ×100. <b><i>Results:</i></b> In all 4 biopsy specimens with AABMR showing cg0, LVSEM revealed GBM duplication not identified by LM. The average percentage of duplicated capillary number per glomerulus with GBM duplication was higher when observed by LVSEM than when observed by LM in all cg1b and cg2 biopsy specimens. <b><i>Conclusion:</i></b> LVSEM revealed early GBM duplication in AABMR. Early GBM duplication might progress in the very early phase of AABMR. GBM duplication was more frequently detected by LVSEM than by LM in biopsy specimens with early chronic, active antibody mediated rejection. Thus, LVSEM may be useful in diagnosis of early transplant glomerulopathy.

Scanning Electron Microscopy of Human Jejunum in Whipple's Disease

1977 ◽  
Vol 35 ◽  
pp. 354-355
Author(s):  
John H. L. Watson ◽  
C. N. Sun
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Lamina Propria ◽  
Brush Border ◽  
Whipple’S Disease ◽  
Whipple's Disease ◽  
Biopsy Specimens ◽  
Brush Borders ◽  
Scanning Electron

That the etiology of Whipple's disease could be bacterial was first suggested from electron micrographs in 1960. Evidence for binary fission of the bacteria, their phagocytosis by histiocytes in the lamina propria, their occurrence between and within the cells of the epithelium and on the brush border of the lumen were reported later. Scanning electron microscopy has been applied by us in an attempt to confirm the earlier observations by the new technique and to describe the bacterium further. Both transmission and scanning electron microscopy have been used concurrently to study the same biopsy specimens, and transmission observations have been used to confirm those made by scanning.The locations of the brush borders, the columnar epithelial cells, the basement membrane and the lamina propria beneath it were each easily identified by scanning electron microscopy. The lamina propria was completely filled with the wiener-shaped bacteria, Fig. 1.

Application of low-vacuum scanning electron microscopy for renal biopsy specimens

2012 ◽  
Vol 208 (9) ◽  
pp. 503-509 ◽  
Author(s):  
Hiroki Miyazaki ◽  
Hiroshi Uozaki ◽  
Akihiro Tojo ◽  
Sayuri Hirashima ◽  
Sumire Inaga ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Biopsy ◽  
Biopsy Specimens ◽  
Scanning Electron

scholarly journals Evaluation of ultrastructural alterations of glomerular basement membrane and podocytes in glomeruli by low-vacuum scanning electron microscopy

2021 ◽  
Author(s):  
Ping Lan ◽  
Dedong Kang ◽  
Akiko Mii ◽  
Yoko Endo ◽  
Masako Tagawa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Assessment Tool ◽  
Periodic Acid ◽  
Renal Pathology ◽  
Blue Staining ◽  
Formalin Fixed Paraffin Embedded ◽  
Scanning Electron

Abstract Background Low-vacuum scanning electron microscopy (LV-SEM) is applied to diagnostic renal pathology. Methods To demonstrate the usefulness of LV-SEM and to clarify the optimal conditions of pathology samples, we investigated the alterations of glomerular basement membrane (GBM) and podocytes in control and experimental active Heymann nephritis (AHN) rats by LV-SEM. Results On week 15 following induction of AHN, spike formation on GBM with diffuse deposition of IgG and C3 developed. Using LV-SEM, diffuse crater-like protrusions were clearly noted three-dimensionally (3D) on surface of GBM in the same specimens of light microscopy (LM) and immunofluorescence (IF) studies only after removal coverslips or further adding periodic acid-silver methenamine (PAM) staining. These 3D ultrastructural findings of GBM surface could be detected in PAM-stained specimens by LV-SEM, although true GBM surface findings could not be obtained in acellular glomeruli, because some subepithelial deposits remained on surface of GBM. Adequate thickness was 1.5–5 μm for 10% formalin-fixed paraffin-embedded (FFPE) and 5–10 μm for the unfixed frozen sections. The foot processes and their effacement of podocytes could be observed by LV-SEM using 10%FFPE specimens with platinum blue (Pt-blue) staining or double staining of PAM and Pt-blue. These findings were obtained more large areas in 2.5% glutaraldehyde-fixed paraffin-embedded (2.5%GFPE) specimens. Conclusion Our findings suggest that LV-SEM is a useful assessment tool for evaluating the alterations of GBM and podocytes in renal pathology using routine LM and IF specimens, as well as 2.5%GFPE specimens.

Procedures for obtaining sectional views of fungal fructifications by scanning electron microscopy

1976 ◽  
Vol 22 (9) ◽  
pp. 1252-1257 ◽  
Author(s):  
M. F. Brown ◽  
H. G. Brotzman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Periodic Acid ◽  
Ceratocystis Fimbriata ◽  
Internal Structures ◽  
Excellent Preservation ◽  
Coating Procedure ◽  
Lecanosticta Acicola ◽  
Host Tissues ◽  
Scanning Electron

Procedures for sectioning fungal fructifications in host tissues or on artificial media are described, which allow observation of internal structures by scanning electron microscopy. Perithecia of Ceratocystis fimbriata and Phyllachora graminis, and telia of Puccinia xanthii showed excellent preservation of exposed structures in sections which were osmium-coated before being dried. While similar preservation was obtained in sectioned acervuli of Lecanosticta acicola and Marssonina juglandis and in pycnidia of Dothiorella ribis and Phomopsis occulta, the mucilaginous substances produced in these fructifications precluded observation of conidiophores. Extraction of mucilage from these sections was accomplished by periodic acid and dilute KOH treatments, followed by an osmium-coating procedure. In such preparations, mucilage was removed, internal structures were preserved, and pertinent characteristics of conidiogenous cells were resolved.

scholarly journals Low-vacuum scanning electron microscopy may allow early diagnosis of human renal transplant antibody-mediated rejection

2020 ◽  
Vol 41 (2) ◽  
pp. 81-90
Author(s):  
Hiroki YOKOYAMA ◽  
Shinichi OKADA ◽  
Yuko YAMADA ◽  
Koichi KITAMOTO ◽  
Sumire INAGA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Transplant ◽  
Early Diagnosis ◽  
Antibody Mediated Rejection ◽  
Scanning Electron

The preparation of glomeruli from renal biopsy specimens for scanning electron microscopy

Pathology ◽  
1982 ◽  
Vol 14 (3) ◽  
pp. 299-302 ◽  
Author(s):  
Wing-ling Ng ◽  
K.F. So ◽  
P.C. So ◽  
H.K. Ngai
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Renal Biopsy ◽  
Biopsy Specimens ◽  
Scanning Electron

scholarly journals Synthesis and Characterisation of a Monolithic Imprinted Column Using a Methacrylic Acid Monomer with Porogen Propanol for Atenolol Analysis

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Aliya Nur Hasanah ◽  
Firdha Senja Maelaningsih ◽  
Fadli Apriliandi ◽  
Akhmad Sabarudin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Methacrylic Acid ◽  
Monolithic Column ◽  
Monolithic Columns ◽  
Selectivity Factor ◽  
Serum Samples ◽  
Average Percentage ◽  
Scanning Electron

A monolithic imprinted atenolol column was constructed by in situ polymerisation using a methacrylic acid monomer and a 1 : 1 (v/v) porogen of propanol: toluene with two template: monomer: crosslinker combinations, namely, MIP 1 (1 : 4 : 20) and MIP 2 (1 : 5 : 20). Physical characterisation of the monolithic columns consisted of permeability testing, Fourier transform infrared (FTIR) testing, surface area analysis (SAA), and scanning electron microscopy (SEM). The permeability value of four monolithic columns was in the good category: MIP 1 (24.01 mD), NIP 1 (56.43 mD), MIP 2 (23.03 mD), and NIP 2 (14.47 mD). The polymerisation process of these four monolithic imprinted columns was carried out perfectly, as shown by the absence of vinyl groups (1000 cm−1 and 900 cm−1) during FTIR testing. Based on SAA testing, the pores of the four polymers were classified as mesopores. The best monolithic column was MIP 1, as seen from the intercolumn and intracolumn reproducibility values and a % RSD <2.0%. The MIP 1 column was selective towards atenolol, as seen from the selectivity factor, imprinting factor (IF), and resolution (Rs) values. The IF values of MIP 1 were atenolol (204.62), metoprolol (3.36), and propranolol (1.27). The Rs value between atenolol and the analogue compounds was 7.23. The MIP 1 column can be used for the analysis of atenolol in blood serum samples with an average percentage recovery rate of 94.88 ± 4.43%.

Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus

Parasitology ◽  
1985 ◽  
Vol 90 (2) ◽  
pp. 357-363 ◽  
Author(s):  
C. D. Nicholls ◽  
D. L. Lee ◽  
M. J. Sharpe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Haemonchus Contortus ◽  
Parasitic Nematodes ◽  
Fibre Optic ◽  
Biopsy Specimens ◽  
Optic Endoscopy ◽  
Surrounding Areas ◽  
Scanning Electron

The abomasum of living sheep infected with 50000 larvae of Haemonchus contortus was examined before and during infection, by means of fibre optic endoscopy. Biopsy specimens were removed from the abomasum of the living sheep and were examined by means of scanning electron microscopy. Changes were noted in the surface structure of the abomasum 2 days after infection and larvae were seen on, and burrowing into, the mucosa. These changes became more pronounced as the infection proceeded, especially after day 10 of the infection when the adult worms had appeared. The behaviour of adult nematodes was observed within the abomasum and on occasions they were seen to move from surrounding areas into an area of haemorrhage, caused by removal of a biopsy specimen. Individual nematodes were removed from the abomasum by means of the biopsy forceps. Fibre optic endoscopy was shown to be a useful tool in the study of parasitic nematodes in vivo.

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