scholarly journals Clonagem do cDNA que codifica a enzima mio-inositol oxigenase (EC 1.13.99.1) de >i/ii/ii/i< L.

2020 ◽  
Author(s):  
Daniela Defávari do Nascimento
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum

Jasmonate response of the Nicotiana tabacum agglutinin promoter in Arabidopsis thaliana

2011 ◽  
Vol 49 (8) ◽  
pp. 843-851 ◽  
Author(s):  
Annelies Delporte ◽  
Nausicaä Lannoo ◽  
Gianni Vandenborre ◽  
Maté Ongenaert ◽  
Els J.M. Van Damme
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum

A detoxification gene in transgenicNicotiana tabacumconfers 2,4-D tolerance

Weed Science ◽  
1999 ◽  
Vol 47 (4) ◽  
pp. 401-404 ◽  
Author(s):  
David I. Last ◽  
Danny J. Llewellyn
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum ◽  
Pisum Sativum ◽  
Histone Gene ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Spray Drift ◽  
Degree Of Protection ◽  
Dioxygenase Gene ◽  
Detoxification Gene

TransgenicNicotiana tabacumwith tolerance to 2,4-D has previously been produced using a bacterial 2,4-D-dioxygenase gene (tfdA) driven by the 35S promoter of cauliflower mosaic virus. Using promoters from thePisum sativumplastocyanin gene (petE) and anArabidopsis thalianahistone gene (H4A), we demonstrate that similar protection from 2,4-D can be obtained in transgenicN. tabacumby targeting expression oftfdAto either meristematic tissues or chloroplast-containing tissues. As with the 35S promoter constructs, the plants are tolerant but not completely resistant; very young seedlings in particular are only slightly protected. However, the levels of tolerance observed could offer a useful degree of protection from accidental spray drift.

scholarly journals Unique Shine–Dalgarno Sequences in Cyanobacteria and Chloroplasts Reveal Evolutionary Differences in Their Translation Initiation

2019 ◽  
Vol 11 (11) ◽  
pp. 3194-3206 ◽  
Author(s):  
Yulong Wei ◽  
Xuhua Xia
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum ◽  
Translation Initiation ◽  
Ribosomal Proteins ◽  
Selection Pressure ◽  
Rna Seq ◽  
Prominent Feature ◽  
Transcriptomic Data ◽  
Reduced Genome ◽  
Cyanobacterial Genes

Abstract Microorganisms require efficient translation to grow and replicate rapidly, and translation is often rate-limited by initiation. A prominent feature that facilitates translation initiation in bacteria is the Shine–Dalgarno (SD) sequence. However, there is much debate over its conservation in Cyanobacteria and in chloroplasts which presumably originated from endosymbiosis of ancient Cyanobacteria. Elucidating the utilization of SD sequences in Cyanobacteria and in chloroplasts is therefore important to understand whether 1) SD role in Cyanobacterial translation has been reduced prior to chloroplast endosymbiosis or 2) translation in Cyanobacteria and in plastid has been subjected to different evolutionary pressures. To test these alternatives, we employed genomic, proteomic, and transcriptomic data to trace differences in SD usage among Synechocystis species, Microcystis aeruginosa, cyanophages, Nicotiana tabacum chloroplast, and Arabidopsis thaliana chloroplast. We corrected their mis-annotated 16S rRNA 3′ terminus using an RNA-Seq-based approach to determine their SD/anti-SD locational constraints using an improved measurement DtoStart. We found that cyanophages well-mimic Cyanobacteria in SD usage because both have been under the same selection pressure for SD-mediated initiation. Whereas chloroplasts lost this similarity because the need for SD-facilitated initiation has been reduced in plastids having much reduced genome size and different ribosomal proteins as a result of host-symbiont coevolution. Consequently, SD sequence significantly increases protein expression in Cyanobacteria but not in chloroplasts, and only Cyanobacterial genes compensate for a lack of SD sequence by having weaker secondary structures at the 5′ UTR. Our results suggest different evolutionary pressures operate on translation initiation in Cyanobacteria and in chloroplast.

Growth modulation effects of CBM2a under the control of AtEXP4 and CaMV35S promoters in Arabidopsis thaliana, Nicotiana tabacum and Eucalyptus camaldulensis

2017 ◽  
Vol 26 (4) ◽  
pp. 447-463 ◽  
Author(s):  
Pornthep Keadtidumrongkul ◽  
Anongpat Suttangkakul ◽  
Phitsanu Pinmanee ◽  
Kanokwan Pattana ◽  
Chokchai Kittiwongwattana ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum ◽  
Eucalyptus Camaldulensis ◽  
Growth Modulation

Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine

Vaccine ◽  
2007 ◽  
Vol 25 (49) ◽  
pp. 8228-8240 ◽  
Author(s):  
Raffaella Greco ◽  
Marie Michel ◽  
Denise Guetard ◽  
Minerva Cervantes-Gonzalez ◽  
Nilla Pelucchi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Nicotiana Tabacum ◽  
Virus Like Particles ◽  
Hiv 1

scholarly journals Aktivitas ACCase mesokarp kelapa sawit dan kloning fragmen gen penyandi ACCase subunit biotin karboksilase ACCase activity of oil palm mesocarp and cloning of gene fragment encoding biotin carboxylase subunit of ACCase

2016 ◽  
Vol 74 (1) ◽  
Author(s):  
Asmini BUDIANI ◽  
Djoko SANTOSO ◽  
Hajrial ASWIDINNOOR ◽  
Antonius SUWANTO
Keyword(s):  
Arabidopsis Thaliana ◽  
Brassica Napus ◽  
Nicotiana Tabacum ◽  
Oil Palm ◽  
Annealing Temperature ◽  
Rt Pcr ◽  
Cdna Fragment ◽  
Gene Encoding ◽  
Conserved Region ◽  
Cloned Cdna

Summary Genetic engineering to produce high yielding oil palm might be done by over expressing gene encoding key enzyme for oil biosynthesis in the oil palm mesocarp, one of which is ACCase. The objective of this research was to analyze ACCase activity of mesocarp from several developmental stages of fruit and to clone conserved region cDNA of gene encoding biotin carboxylase subunit of ACCase (BC-htACCase) from oil palm mesocarp. Activity of ACCase was analyzed by HPLC. Amplification of cDNA was done by means of reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate heterologous primer on several annealing temperature and MgCl2 concentration. The cDNA fragment of RT-PCR product was cloned, sequenced and analyzed to confirm that the cloned cDNA was conserved region of BC-htACCase. The result showed that ACCase activity increased from the 14 week to the 20 week-old fruit, and then decreased. Using heterologous degenerate primers, cDNA fragments of BC-htACCase conserved region (469 bp) can be specifically amplified at 60 oC annealing temperature with 2 mM MgCl2 concentration.The result of BlastX analysis showed that the sequence of cloned cDNA fragment was highly homologous with the conserved region of BC-htACCase from Glycine max, Arabidopsis thaliana, Nicotiana tabacum,  and Brassica napus with 243, 237, 236, 231 bit score, and E. value 2e-63, 1e-61, 2e-61 and 5e-60, respectively. Ringkasan Rekayasa genetika untuk menghasilkan bibit kelapa sawit berdaya hasil tinggi dapat ditempuh dengan meningkatkan ekspresi gen penyandi enzim kunci biosintesis minyak pada kelapa sawit, salah satunya adalah ACCase. Tujuan penelitian ini adalah menguji aktivitas ACCase mesokarp beberapa tahap perkem-bangan buah sawit dan mengklon fragmen cDNA daerah konservatif gen penyandi ACCase heteromerik subunit biotin karbok-silase (BC-htACCase) dari mesokarp buah sawit. Aktivitas ACCase dianalisis dengan HPLC. Amplifikasi cDNA dilakukan dengan teknik RT-PCR menggunakan primer degene-rate heterologus pada berbagai suhu penempelan dan konsentrasi MgCl2. Fragmen cDNA hasil RT-PCR diklon, disekuen dan dianalisis untuk mengkonfirmasi bahwa cDNA terklon adalah daerah konservatif BC-htACCase. Hasil penelitian menunjukkan bahwa aktivitas ACCase meningkat dari buah berumur 14 minggu hingga buah berumur  20 minggu, kemudian menurun kembali Dengan primer degenerate heterologus, fragmen cDNA daerah konservatif BC-htACCase  (469 pb) dapat diamplifikasi secara spesifik pada suhu penempelan 60 oC dan konsentrasi MgCl2 2 mM. Hasil analisis BlastX dari sekuen DNA fragmen terklon menunjuk-kan bahwa sekuen tersebut mempunyai homologi tinggi antara lain dengan gen penyandi BC-htACCase dari Glycine max, Arabidopsis thaliana, Nicotiana tabacum dan Brassica napus, masing-masing dengan skor 243, 237, 236, 231 bit, dan E. value 2e-63, 1e-61, 2e-61 dan 5e-60.

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