Arterial Gene Therapy for Therapeutic Angiogenesis in Patients With Peripheral Artery Disease

Circulation ◽  
1995 ◽  
Vol 91 (11) ◽  
pp. 2687-2692 ◽  
Author(s):  
Jeffrey M. Isner ◽  
Kenneth Walsh ◽  
James Symes ◽  
Ann Pieczek ◽  
Satoshi Takeshita ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Therapeutic Angiogenesis ◽  
Peripheral Artery ◽  
Artery Disease

Therapeutic angiogenesis for peripheral artery disease: Gene therapy

VASA ◽  
2007 ◽  
Vol 36 (3) ◽  
pp. 165-173 ◽  
Author(s):  
Nikol
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Animal Experiments ◽  
Therapeutic Angiogenesis ◽  
Phase Iii ◽  
Peripheral Artery ◽  
Prevalent Disease ◽  
Advanced Stages ◽  
Artery Disease ◽  
Novel Strategy

Peripheral artery disease is a highly prevalent disease which is characterised by a high unmet medical need particularly in the more advanced stages of disease. Recent advances in the knowledge of the complex regulation of angiogenesis and arteriogenesis and ways to its induction offer hope for a novel strategy that is based on the generation of such new vessels. This strategy termed "therapeutic angiogenesis" is a concept based on the use of angiogenic factors or stem cells or their combination to promote neovascularisation for the treatment of ischaemic tissues. This article reviews both regulation of angiogenesis and the development of therapeutic strategies based on this knowledge using gene therapy. This includes knowledge from animal experiments as well as from phase I and phase II clinical trials. This information may be particularly important at a time when angiogenesis gene therapy enters the stage of phase III clinical testing hopefully leading to the first time approval of this completely new class of drug in the near future. Following articles of this series will review therapeutic angiogenesis approaches based on cytokine therapy and stem cell therapy.

scholarly journals Gene Therapy and Cell-Based Therapies for Therapeutic Angiogenesis in Peripheral Artery Disease

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Munehisa Shimamura ◽  
Hironori Nakagami ◽  
Hiroshi Koriyama ◽  
Ryuichi Morishita
Keyword(s):  
Gene Therapy ◽  
Growth Factor ◽  
Peripheral Artery Disease ◽  
Mononuclear Cells ◽  
Therapeutic Angiogenesis ◽  
Novel Therapies ◽  
Peripheral Artery ◽  
Peripheral Blood Mononuclear ◽  
Cell Based Therapy ◽  
Artery Disease

Gene therapy and cell-based therapy have emerged as novel therapies to promote therapeutic angiogenesis in critical limb ischemia (CLI) caused by peripheral artery disease (PAD). Although researchers initially focused on gene therapy using proangiogenic factors, such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and hepatocyte growth factors (HGF), cell therapy using bone marrow mononuclear cells (BMMNCs), mesenchymal stem cells (BMMSCs), G-CSF-mobilized peripheral blood mononuclear cells (M-PBMNCs), and endothelial progenitor cells (EPCs) have also been extensively studied. Based on the elaborate studies and favorable results of basic research, some clinical phase I/II trials have been performed, and the results demonstrate the safety of these approaches and their potential for symptomatic improvement in CLI. However, the phase 3 clinical trials have thus far been limited to gene therapy using the HGF gene. Further studies using well-designed larger placebo-controlled and long-term randomized control trials (RCTs) will clarify the effectiveness of gene therapy and cell-based therapy for the treatment of CLI. Furthermore, the development of efficient gene transfer systems and effective methods for keeping transplanted cells healthy will make these novel therapies more effective and ease the symptoms of CLI.

HGF Gene Therapy for Therapeutic Angiogenesis in Peripheral Artery Disease

2017 ◽  
pp. 133-144 ◽  
Author(s):  
Jun Muratsu ◽  
Fumihiro Sanada ◽  
Yoshiaki Taniyama ◽  
Yuka Ikeda-Iwabu ◽  
Rei Otsu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Therapeutic Angiogenesis ◽  
Peripheral Artery ◽  
Artery Disease

scholarly journals Targets and delivery methods for therapeutic angiogenesis in peripheral artery disease

2012 ◽  
Vol 17 (3) ◽  
pp. 174-192 ◽  
Author(s):  
Geoffrey O Ouma ◽  
Rebecca A Jonas ◽  
M Haris U Usman ◽  
Emile R Mohler
Keyword(s):  
Peripheral Artery Disease ◽  
Therapeutic Angiogenesis ◽  
Peripheral Artery ◽  
Delivery Methods ◽  
Artery Disease

Gene Therapy in Peripheral Artery Disease

2004 ◽  
Vol 4 (3) ◽  
pp. 295-300 ◽  
Author(s):  
G. Melillo ◽  
F. Serino ◽  
C. Cirielli ◽  
M. Capogrossi
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Peripheral Artery ◽  
Artery Disease

Abstract 17955: Thereapeutic Angiogenesis Induced by Human Trx-1 Gene in Mice Hind Limb Ischemia Model: Preclinical Study for Treatment of Peripheral Artery Disease

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Mahesh Thirunavukkarasu ◽  
Inam A Shaikh ◽  
Vaithinathan Selvaraju ◽  
J.Alexandar Palesty ◽  
Nilanjana Maulik
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Femoral Artery ◽  
Hind Limb ◽  
Blood Perfusion ◽  
Limb Ischemia ◽  
Heme Oxygenase 1 ◽  
Peripheral Artery ◽  
Artery Ligation ◽  
Artery Disease

Introduction: Peripheral artery disease affects 12-20% Americans over the age of 60. Thioredoxin-1 (Trx-1) is a class of small redox proteins. We have demonstrated earlier that Trx-1 reduces oxidative stress resulting in less inflammation and increased angiogenesis in cardiac muscle via heme oxygenase-1 (HO-1) and VEGF after myocardial infarction. In the current study, we evaluate the effect of Trx-1 on post-ischemic hindlimb recovery. Methods: Peripheral artery disease was mimicked using a hindlimb ischemia (HLI) model. Wild type (WT) and Trx-1 transgenic (Trx-1Tg/+) mice (8-12 weeks old) were subjected to femoral artery ligation. Following surgery, mice were observed for 5 weeks. Serial laser doppler images were obtained, and perfusion ratios between the ischemic and non-ischemic limbs were calculated at set time intervals. The perfusion ratios were compared between WT and Trx-1Tg/+ groups. Immunohistochemical analysis of the skeletal muscle was performed to quantify the extent of fibrosis, capillary and arteriolar density 35 days after surgery. In addition, another set of experiments was designed with Ad.Trx-1 gene therapy after femoral artery ligation to study the molecular mechanism of neovascularization with Trx-1. Results: The recovery of hind limb perfusion was significantly increased in Trx-1Tg/+ mice at day 7 (0.19 ± 0.03 vs. 0.36 ± 0.07 (n=12-9), day-21 (0.37 ± 0.05 vs. 0.62 ± 0.03 (n=12-9), and day 28 (0.40 ± 0.04 vs. 0.79 ± 0.04 (n=10-9); p<0.05). Capillary density [1265 ± 87.8 vs. 762.4 ± 86.6 counts/mm2 ; (n=5); p<0.05] and arteriolar density [36.2 ± 2.96 vs. 22± 1.33 counts/mm2 ; (n=5); p<0.05] staining showed significant increase in Trx-1Tg/+ mice as compared to WT mice. Picrosirrus Red and immunofluorescence staining showed decreased fibrosis [8.3 ± 0.46 vs. 22.2 ± 1.04 (n=5); p<0.0001] and increased HO-1 expression respectively in Trx-1Tg/+ mice group as compared to WT mice, respectively. Trx-1 gene therapy study also revealed by Western blot analysis, increased Trx-1 (4.2 fold) and HO-1 (8.2 fold) expression in Ad.Trx-1-HLI as compared to Ad.LacZ-HLI. Conclusions: Our results suggest that Trx-1 is a potential therapeutic agent to increase blood perfusion and angiogenesis for the treatment of critical limb ischemia patients.

Therapeutic angiogenesis for revascularization in peripheral artery disease

Gene ◽  
2013 ◽  
Vol 525 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Anna Grochot-Przeczek ◽  
Jozef Dulak ◽  
Alicja Jozkowicz
Keyword(s):  
Peripheral Artery Disease ◽  
Therapeutic Angiogenesis ◽  
Peripheral Artery ◽  
Artery Disease

scholarly journals Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease

2004 ◽  
Vol 36 (4) ◽  
pp. 336-344 ◽  
Author(s):  
Hyun-Joong Kim ◽  
Shin Yi Jang ◽  
Joong-Il Park ◽  
Jonghoe Byun ◽  
Dong-Ik Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Peripheral Artery Disease ◽  
Vascular Endothelial ◽  
Peripheral Artery ◽  
Angiogenic Gene ◽  
Artery Disease ◽  
Endothelial Growth Factor

Gene therapy in peripheral artery disease

2015 ◽  
Vol 15 (3) ◽  
pp. 381-390 ◽  
Author(s):  
Fumihiro Sanada ◽  
Yoshiaki Taniyama ◽  
Yasuhiro Kanbara ◽  
Rei Otsu ◽  
Yuka Ikeda-Iwabu ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Peripheral Artery Disease ◽  
Peripheral Artery ◽  
Artery Disease
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