scholarly journals Rabbit heart fatty acid-binding protein. Isolation, characterization, and application of a monoclonal antibody.

1989 ◽  
Vol 65 (4) ◽  
pp. 981-988 ◽  
Author(s):  
A A Knowlton ◽  
R E Burrier ◽  
P Brecher
Keyword(s):  
Monoclonal Antibody ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Rabbit Heart ◽  
Protein Isolation ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Development of a therapeutic monoclonal antibody that targets secreted fatty acid–binding protein aP2 to treat type 2 diabetes

2015 ◽  
Vol 7 (319) ◽  
pp. 319ra205-319ra205 ◽  
Author(s):  
M. Furkan Burak ◽  
Karen E. Inouye ◽  
Ariel White ◽  
Alexandra Lee ◽  
Gurol Tuncman ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Monoclonal Antibody ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Therapeutic Monoclonal Antibody

Preparation and characterization of a monoclonal antibody to the fatty acid binding protein (MFABP)

1989 ◽  
Vol 9 ◽  
pp. S25 ◽  
Author(s):  
H.E. Diede ◽  
M. Schrader ◽  
B. Zimmerbeutel ◽  
H. Hoppeler ◽  
M. Manns ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of Fasciola gigantica

2002 ◽  
Vol 105 (2) ◽  
pp. 119-129 ◽  
Author(s):  
A Sirisriro ◽  
R Grams ◽  
S Vichasri-Grams ◽  
P Ardseungneon ◽  
V Pankao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fasciola Gigantica ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Immunodiagnostic monoclonal antibody-based sandwich ELISA of fasciolosis by detection ofFasciola giganticacirculating fatty acid binding protein

Parasitology ◽  
2016 ◽  
Vol 143 (11) ◽  
pp. 1369-1381 ◽  
Author(s):  
PANAT ANURACPREEDA ◽  
RUNGLAWAN CHAWENGKIRTTIKUL ◽  
PRASERT SOBHON
Keyword(s):  
Monoclonal Antibody ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Sandwich Elisa ◽  
Parasite Burden ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Low Sensitivity

SUMMARYUp to now, parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Hence, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden. In the present study, a monoclonal antibody (MoAb) against recombinantFasciola giganticafatty acid binding protein (rFgFABP) has been produced. As well, a reliable sandwich enzyme-linked immunosorbent assay (sandwich ELISA) has been developed for the detection of circulating FABP in the sera of mice experimentally and cattle naturally infected withF. gigantica. MoAb 3A3 and biotinylated rabbit anti-recombinant FABP antibody were selected due to their high reactivities and specificities. The lower detection limit of sandwich ELISA was 5 pg mL−1, and no cross-reaction with other parasite antigens was observed. This assay could detectF. giganticainfection from day 1 post infection. In experimental mice, the sensitivity, specificity and accuracy of this assay were 93·3, 100 and 98·2%, while in natural cattle they were 96·7, 100 and 99·1%. Hence, this sandwich ELISA method showed high efficiencies and precisions for diagnosis of fasciolosis byF. gigantica.

Verminderte Adipocyte fatty acid binding protein (AFABP)-Spiegel bei Patienten mit chronischer Pankreatitis

2011 ◽  
Vol 49 (08) ◽  
Author(s):  
L Selig ◽  
T Ebert ◽  
M Reiche ◽  
D Krehl ◽  
U Lossner ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein
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