scholarly journals TRPV4 (Transient Receptor Potential Vanilloid 4) Channel–Dependent Negative Feedback Mechanism Regulates G q Protein–Coupled Receptor–Induced Vasoconstriction

2018 ◽  
Vol 38 (3) ◽  
pp. 542-554 ◽  
Author(s):  
Kwangseok Hong ◽  
Eric L. Cope ◽  
Leon J. DeLalio ◽  
Corina Marziano ◽  
Brant E. Isakson ◽  
...  
Keyword(s):  
Negative Feedback ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Feedback Mechanism ◽  
Transient Receptor Potential Vanilloid ◽  
Negative Feedback Mechanism ◽  
Q Protein ◽  
Transient Receptor ◽  
Channel Dependent

scholarly journals Functional basis for calmodulation of the TRPV5 calcium channel

2021 ◽  
Author(s):  
Sara R Roig ◽  
Niky Thijssen ◽  
Merijn van Erp ◽  
Jack Fransen ◽  
Joost G Hoenderop ◽  
...  
Keyword(s):  
Single Molecule ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Feedback Mechanism ◽  
Negative Feedback Mechanism ◽  
Biochemical Assays ◽  
Selective Channel ◽  
Cam Regulation ◽  
Transient Receptor ◽  
Microscopy Techniques

AbstractWithin the transient receptor potential (TRP) superfamily of ion channels, TRPV5 is a highly Ca2+-selective channel important for active reabsorption of Ca2+ in the kidney. Its channel activity is controlled by a negative feedback mechanism involving calmodulin (CaM) binding. Combining advanced microscopy techniques and biochemical assays, this study characterized the dynamic bilobal CaM regulation and binding stoichiometry. We demonstrate for the first time that functional (full-length) TRPV5 interacts with CaM in the absence of Ca2+, and this interaction is intensified at increasing Ca2+ concentrations sensed by the CaM C-lobe that achieves channel pore blocking. Channel inactivation occurs without CaM N-lobe calcification. Moreover, we reveal a 1:2 stoichiometry of TRPV5:CaM binding by implementing single molecule photobleaching counting, a technique with great potential for studying TRP channel regulation. In conclusion, our study proposes a new model for CaM- dependent regulation – calmodulation – of the Ca2+-selective TRPV5 that involves apoCaM interaction and lobe-specific actions.

scholarly journals Negative-Feedback Loop Attenuates Hydrostatic Lung Edema via a cGMP-Dependent Regulation of Transient Receptor Potential Vanilloid 4

2008 ◽  
Vol 102 (8) ◽  
pp. 966-974 ◽  
Author(s):  
Jun Yin ◽  
Julia Hoffmann ◽  
Stephanie M. Kaestle ◽  
Nils Neye ◽  
Liming Wang ◽  
...  
Keyword(s):  
Negative Feedback ◽  
Feedback Loop ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Lung Edema ◽  
Negative Feedback Loop ◽  
Transient Receptor Potential Vanilloid ◽  
Transient Receptor
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