Abstract 298: Cytochrome P4501A1 Contributes to Nitric Oxide-Dependent Vasodilation and Blood Pressure Lowering on an Omega-3 Polyunsaturated Fatty Acid-Enriched Diet

Hypertension ◽  
2012 ◽  
Vol 60 (suppl_1) ◽  
Author(s):  
Larry N Agbor ◽  
Mary T Walsh ◽  
Mary K Walker
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Mesenteric Arteries ◽  
Omega 3 ◽  
Blood Pressure Lowering ◽  
Cytochrome P4501a1 ◽  
Pressure Lowering ◽  
Normal Chow ◽  
Blood Pressure Responses

Cytochrome P4501A1 (CYP1A1) stereospecifically metabolizes omega-3 polyunsaturated fatty acids (n-3 PUFA) to potent vasodilators in vitro. We have previously reported that CYP1A1 knockout (KO) mice are hypertensive and exhibit significantly reduced vasodilatory responses to the n-3 PUFAs, eicosapentaenoic and docosahexaenoic acids. We next tested the hypothesis that CYP1A1 KO mice would exhibit significantly different vascular and blood pressure responses to diets enriched in n-3 versus n-6 PUFAs. CYP1A1 wildtype (WT) and KO mice were fed normal chow or diets enriched in either n-3 or n-6 PUFAs for 2 mo, and then blood pressure was assessed by radiotelemetry ± nitric oxide synthase (NOS) inhibitor (NG-nitro-L-Arginine). Acetylcholine (Ach)-mediated vasodilation was assessed in first order mesenteric arteries, and endothelial NOS and phospho-eNOS were measured in the aorta. We found that an n-3 enriched diet significantly reduced mean arterial pressure (MAP) in CYP1A1 KO mice (Chow: 116.0 ± 1.2; n-3 diet: 107.6 ± 1.5, p<0.05) with no effect in WT mice (Chow: 103.0 ± 0.9; n-3 diet: 105.0 ± 2.5). In contrast, an n-6 enriched diet significantly increased MAP in WT mice (Chow: 103.0 ± 0.9; n-6: 118.2 ± 4.1, p<0.05) with no effect in KO mice (Chow: 116.0 ± 1.2; n-6: 115.3 ± 1.5). Interestingly, NOS inhibition increased blood pressure significantly more in the CYP1A1 WT mice (+16 ± 0.5 mmHg) than KO mice (+11 ± 0.6, p<0.002) on an n-3 diet, but resulted in similar increases in blood pressure in both genotypes on an n-6 diet (WT: +11 ± 1.8; KO: +11 ± 0.8). In addition, CYP1A1 KO mice on an n-3 enriched diet exhibited significantly reduced Ach-dependent dilation in mesenteric arteries and reduced expression of aortic phospho-eNOS, compared to WT. However, neither of these endpoints were altered in KO mice on an n-6 diet, compared to WT. Taken together, these data suggest that CYP1A1 contributes to the NO-mediated vasodilation and blood pressure lowering benefits derived from dietary n-3 PUFAs.

Blood pressure-lowering effect of dietary (−)-epicatechin administration in L-NAME-treated rats is associated with restored nitric oxide levels

2012 ◽  
Vol 53 (10) ◽  
pp. 1894-1902 ◽  
Author(s):  
Maria C. Litterio ◽  
Grayson Jaggers ◽  
Gulcin Sagdicoglu Celep ◽  
Ana M. Adamo ◽  
Maria A. Costa ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Blood Pressure Lowering ◽  
Blood Pressure Lowering Effect ◽  
Lowering Effect ◽  
Pressure Lowering

scholarly journals Presence of antioxidants and nitric-oxide precursors in Mimosa pudica extract

2021 ◽  
Vol 7 (3) ◽  
pp. 104-108
Author(s):  
Tapas Pramanik ◽  
Tapas Kumar Sur
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Radical Scavenging ◽  
Reduce Blood Pressure ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Mimosa Pudica ◽  
Blood Pressure Lowering ◽  
Pressure Lowering

Blood pressure lowering effect of Mimosa pudica induced by dieresis was reported earlier. As a diuretic it enhances urine outflow, decreases plasma volume, venous return; and thereby, reduce blood pressure. Besides the diuretic agent, some other blood pressure lowering substance may also be present in Mimosa pudica. Present study was undertaken to reveal the presence of antioxidants and nitrite in Mimosa pudica extract, which may help to reduce blood pressure. Methanolic extract of Mimosa pudica (using 80% methanol) was lyophilised to obtain dried Mimosa pudica Extract (MPE). For Total phenolic content estimation Folin’s method and for estimation of flavonoids, Aluminium chloride method were followed. The radical scavenging and superoxide anion radical scavenging activity were measured following standardised methods. Nitrite content of MPE at different dilutions (10-100 µl in methanol) was measured following standardised procedure keeping sodium nitrite as the standard. Present study noted presence of favonoids and phenolic compounds and also noted antioxidant property in the aforesaid extract that exhibited DPPH+ and superoxide scavenging activities. Besides that, this study also revealed formation of nitrites in the extract of Mimosa pudica in a dose dependent manner. Nitrite is the precursor of nitric oxide (NO). NO is a potent vasodilator that decreases blood pressure. Present study indicated the presence of both antioxidants and nitrites in Mimosa pudica extract; both of which have blood pressure lowering properties indicating it as a blood pressure lowering agent; and helpful in the maintenance of vascular health.

Blood Pressure-Lowering Effects of Alacalase-Hydrolyzed Camellia Seed Hull In Vitro and in Spontaneous Hypertensive Rats

2017 ◽  
Vol 20 (7) ◽  
pp. 720-723 ◽  
Author(s):  
Ho-Jeong Lim ◽  
Mi-So Kim ◽  
Da-Som Kim ◽  
Young Jun Kim ◽  
Jin Hyup Lee ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Pressure Lowering ◽  
Hypertensive Rats ◽  
Pressure Lowering ◽  
Seed Hull

scholarly journals Addition of a Nitric Oxide Donor to an Angiotensin II Type 1 Receptor Blocker May Cancel Its Blood Pressure-Lowering Effects

2015 ◽  
Vol 56 (6) ◽  
pp. 656-660 ◽  
Author(s):  
Eiji Yahiro ◽  
Shin-ichiro Miura ◽  
Yasunori Suematsu ◽  
Yoshino Matsuo ◽  
Tadaaki Arimura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Angiotensin Ii ◽  
Receptor Blocker ◽  
Nitric Oxide Donor ◽  
Blood Pressure Lowering ◽  
Pressure Lowering

scholarly journals Plasma and Tissue Bioavailability and Blood Pressure Lowering Effects of Omega-3 Polyunsaturated Fatty Acids from Commonly Eaten, Naturally Enriched, Foods

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Alice Stanton ◽  
Kathleen Shortall ◽  
Thora El-Sayed ◽  
Margaret Brennan ◽  
Fahad Buskandar ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Chicken Meat ◽  
Red Cell ◽  
Omega 3 ◽  
Blood Pressure Lowering ◽  
Oily Fish ◽  
Pressure Lowering ◽  
Meat And Eggs

AbstractGreater consumption of oily fish, and elevated plasma levels of the long-chain marine-derived omega-3 polyunsaturated fatty acids (PUFAs), docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), have been strongly and consistently associated with a reduced incidence of heart attacks, strokes, cognitive decline, Alzheimer's disease and cancers. Despite international guidelines recommending eating oily fish at least once per week (> 250mg/day DHA and EPA), many people do not eat fish, and only a small minority of the world's population have optimal omega-3-PUFA plasma and tissue levels. Hence, in this project, we studied the bioavailability and blood pressure lowering effects of recently developed alternatives to oily fish, namely chicken-meat and eggs naturally enriched with algae-sourced omega-3-PUFAs.This was a double-blinded, controlled, randomised trial. Using a 2X2 factorial design, 161 healthy participants were randomized to eat at least 3 portions/week of omega-3-PUFA enriched (or control) chicken-meat, and to eat at least 3 omega-3-PUFA enriched (or control) eggs/week, for 6 months.Compared to control foods, eating enriched eggs, enriched chicken-meat and the combination for 6 months, resulted in plasma EPA increments (μg/g) of 3.1[2.8], 8.8[2.9]* & 4.7[2.9], plasma DHA increments (μg/g) of 5.1[3.7], 9.3[3.8]* & 13.1[3.9]*, and omega-3-index (sum of EPA + DHA as a percentage of total fatty acids in erythrocytes, %) increments of 0.9[0.3]*, 0.7[0.4]* & 1.5[0.4]*, respectively. Mean 24-hour ambulatory blood pressure (systolic/diastolic, mmHg) declined with the enriched foods 0.3[1.3]/-0.2[1.0], -0.7[1.4]/-1.4[1.0], & -1.9[1.4]/-2.9[1.0]*, respectively. Data provided as mean difference[SEM], and * denotes p < 0.05.Regular consumption of omega-3-PUFA enriched chicken-meat and eggs resulted in statistically significant and clinically relevant increases in plasma and red cell levels of DHA and EPA. Both plasma and red cell bioavailability were similar to those previously described for oily fish. Furthermore, blood pressure, an important biomarker of cardiovascular health, decreased in those eating the omega-3-PUFA enriched foods. Omega-3-PUFA enriched chicken-meat and eggs offers consumers an attractive additional alternative to eating oily fish. Unlike many lifestyle interventions, long-term large population health benefits are not dependent on willingness of people to make long-lasting dietary changes, but on the widespread availability of a range of inexpensive, commonly eaten, naturally enriched foods.

scholarly journals Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Kyung-Hyun Cho ◽  
Suk-Jeong Kim ◽  
Dhananjay Yadav ◽  
Jae-Yong Kim ◽  
Jae-Ryong Kim
Keyword(s):  
Blood Pressure ◽  
Cholesterol Efflux ◽  
Blood Pressure Lowering ◽  
Blood Pressure Lowering Effect ◽  
Transfer Protein ◽  
Hdl Functionality ◽  
Pressure Lowering ◽  
Double Blinded

Policosanol has been reported to improve blood pressure, lipid profile, and HDL functionality via inhibition of cholesteryl ester transfer protein (CETP) both in vitro and in vivo in zebrafish and human models. However, there are limited reports and randomized, double-blinded trials on policosanol that could advocate the blood pressure-lowering effect in prehypertensive participants. Therefore, we performed in vitro, in vivo, and ex vivo experiments to provide more substantial and concrete data on the blood pressure-lowering effect of policosanol. Consumption of policosanol for 8 weeks enhanced plasma antioxidant activity. In the policosanol group, plasma total cholesterol (TC) and triglyceride (TG) levels were reduced up to 20% and 14%, respectively, and HDL-C level was elevated up to 1.3-fold compared to that at week 0. TG/HDL-C and cholesteryl ester transfer protein (CETP) activities were reduced up to 36% and 20%, respectively. Uptake of oxidized LDL in macrophages was reduced as oxidized species levels were reduced, and HDL2-associated paraoxonase activities were enhanced by 60% compared to those at week 0. Encapsulation of policosanol into reconstituted HDL (PCO-rHDL) enhanced cholesterol efflux activity and insulin secretion capacity. In conclusion, consumption of policosanol for 8 weeks in healthy female subjects resulted in lowered blood pressure and CETP activity via elevation of HDL/apoA-I contents and enhancement of HDL functionalities, including cholesterol efflux and insulin secretion. These functional enhancements of HDL can contribute to the prevention of aging-related diseases, hypertension, and stroke.

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