scholarly journals Baujardia mirabilis gen. n., sp. n. from pitcher plants and its phylogenetic position within Panagrolaimidae (Nematoda: Rhabditida)

Nematology ◽  
2003 ◽  
Vol 5 (3) ◽  
pp. 405-420 ◽  
Author(s):  
Rita Van Driessche ◽  
Irma Tandingan De Ley ◽  
Paul De Ley ◽  
Hendrik Segers ◽  
Wim Bert

AbstractMeasurements, line drawings and scanning electromicrographs are provided of Baujardia mirabilis gen. n., sp. n., isolated from pitcher fluid of Nepenthes mirabilis from Thailand. The new genus differs from all known nematodes in having two opposing and offset spermatheca-like pouches at the junction of oviduct and uterus. It also differs from most known Rhabditida in having four cephalic setae instead of papillae. Phylogenetic analysis of small subunit rDNA sequence data robustly places the new genus within Panagrolaimidae as a sister taxon to Panagrellus. These unusual nematodes resemble Panagrellus in body size (1.8-2.7 mm in females, 1.3-1.9 mm in males) and in the monodelphic, prodelphic female reproductive system with thickened vaginal walls and prominent postvulval sac. However, they differ from Panagrellus in the characters mentioned above, in their comparatively longer stegostom and in the shape of the male spicules. Because of its aberrant characters, inclusion of this new genus in Panagrolaimidae requires changes to the family diagnosis.

2021 ◽  
Vol 12 ◽  
Author(s):  
Qi Gao ◽  
Chen Shao ◽  
Qiuyue Tang ◽  
Jingbao Li

The morphology and morphogenesis of Pseudosincirra longicirrata nov. gen. and nov. comb., isolated from southern China, were investigated with living observation and protargol staining. Our population is similar to the original population in living characteristics and ciliary patterns. The main determinable morphogenetic features of P. longicirrata nov. comb. are the presence of five frontoventral-transverse cirral anlagen (FVT-anlagen) and a dorsomarginal kinety anlage. According to the origin of FVT-anlagen IV and V in proter, it can be determined that P. longicirrata nov. comb. possesses two frontoventral rows and one right marginal row. Hence, a new genus, Pseudosincirra nov. gen., is proposed, and the diagnosis of P. longicirrata nov. comb. is improved. The new genus is diagnosed as follows: adoral zone of membranelles and undulating membranes is in a Gonostomum pattern; there are three enlarged frontal cirri, one buccal cirrus, and one parabuccal cirrus; postperistomial cirrus and transverse cirri are lacking; there are two more or less long frontoventral rows and one right and two or more left marginal rows; cirri within all rows very widely spaced; dorsal kinety pattern is of Urosomoida type, that is, three dorsal kineties and one dorsomarginal kinety; and caudal cirri are present. Phylogenetic analyses based on the small subunit ribosomal (SSU rDNA) sequence data indicate that P. longicirrata nov. comb. clusters with Deviata and Perisincirra. It is considered that Pseudosincirra nov. gen. and Perisincirra paucicirrata should be assigned to the family Deviatidae; fine cirri, and cirri within all rows being relatively widely spaced, should be considered as plesiomorphies of Deviatidae; and Deviatidae is closely related to Dorsomarginalia or Strongylidium–Hemiamphisiella–Pseudouroleptus.


1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.


Zootaxa ◽  
2007 ◽  
Vol 1461 (1) ◽  
pp. 25-37 ◽  
Author(s):  
SERGEY A. BELOKOBYLSKIJ ◽  
ALEJANDRO ZALDIVAR-RIVERÓN ◽  
VIRGINIA LEÓN-REGAGNON ◽  
DONALD L.J. QUICKE

A new genus and species of Lysitermini, Atritermus pedestris Belokobylskij, Zaldivar-Riverón & Quicke, are described from Madagascar. The taxonomic placement of the new genus within Lysiterminae is discussed based on a phylogenetic analysis using 28S rDNA gene sequences. A key to world genera of Lysitermini is provided.Un nuevo género y especie de Lysitermini, Atritermus pedestris Belokobylskij, Zaldivar-Riverón & Quicke, son descritos para Madagascar. La ubicación taxonómica del nuevo género dentro de Lysiterminae es discutida con base en un análisis filogenético usando secuencias del gen ribosomal 28S. Se presenta una clave para los géneros de Lysitermini del mundo.


Phytotaxa ◽  
2013 ◽  
Vol 105 (1) ◽  
pp. 11 ◽  
Author(s):  
HIRAN A. ARIYAWANSA ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
SAMANTHA C.KARUNARATHNE ◽  
EKACHAI CHUKEATIROTE ◽  
ALI H. BAHKALI ◽  
...  

Deniquelata barringtoniae gen. et sp. nov. (Montagnulaceae) forms numerous ascomata on distinct zonate leaf spots of Barringtonia asiatica (Lecythidaceae). We isolated this taxon and sequenced the 18S and 28S nrDNA. The result of phylogenetic analysis based on 18S and 28S nrDNA sequence data indicate that the genus belongs in the family Montagnulaceae, Dothideomycetes, Ascomycota. The ascomata are immersed, dark brown to black, with bitunicate asci and brown, muriform ascospores. Deniquelata is distinguished from the other genera in Montagnulaceae based on its short, broad, furcate and pedicellate asci, verruculose ascospores with short narrow pseudoparaphyses with parasitic naturee and this is also supported by molecular data. A new genus and species is therefore introduced to accommodate this taxon. We used isolates of this species to show via pathogenicity testing that the taxon is able to cause leaf spots when leaves are pin pricked.


2012 ◽  
Vol 62 (Pt_11) ◽  
pp. 2710-2716 ◽  
Author(s):  
Yan-Ru Cao ◽  
Yi Jiang ◽  
Rong-Xian Jin ◽  
Li Han ◽  
Wen-Xiang He ◽  
...  

A novel actinobacterium, designated strain YIM 100590T, was isolated from Panthera tigris amoyensis faeces collected from Yunnan Wild Animal Park in Yunnan province, south-west China. Phylogenetic analysis based on 16S rRNA gene sequence data showed that strain YIM 100590T is a member of the family Micrococcaceae . Cells were coccoid to oval (0.7–1.5 µm in diameter) occurring singly or in clusters. Growth was observed at 10–37 °C (optimum 28 °C) and at pH 7.0–11.0 (optimum pH 8.0). The major fatty acids were iso-C15 : 0 (32.22 %), anteiso-C15 : 0 (31.64 %) and iso-C16 : 0 (17.38 %). The peptidoglycan was of A4α type (l-Lys–Gly–l-Glu). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, dimannosyl diacylglycerol, an unknown glycolipid and two unknown phospholipids. The quinone system comprised menaquinones MK-7 (91.9 %) and MK-8 (8.3 %). The DNA G+C content of strain YIM 100590T was 56.2 mol%. Chemotaxonomic data indicated that the strain belongs to the family Micrococcaceae . On the basis of morphological and chemotaxonomic data and phylogenetic analysis, strain YIM 100590T is considered to represent a novel species of a new genus within the family Micrococcaceae , for which the name Enteractinococcus coprophilus gen. nov., sp. nov. is proposed. The type strain of Enteractinococcus coprophilus is YIM 100590T ( = DSM 24083T = JCM 17352T). Yaniella fodinae DSM 22966T was transferred to the new genus as Enteractinococcus fodinae comb. nov. (type strain G5T = DSM 22966T = JCM 17931T = MTCC 9846T).


Phytotaxa ◽  
2017 ◽  
Vol 291 (1) ◽  
pp. 1 ◽  
Author(s):  
NÁDIA ROQUE ◽  
SILVANA C. FERREIRA ◽  
CÁSSIO VAN DEN BERG

Asteraceae is the family with the highest species number in the rocky fields (campos rupestres) of the Chapada Diamantina, Bahia, Brazil. On the basis of several collections from this area, we are proposing a new genus of Asteraceae based on morphology and phylogeny, to accommodate a species newly described here. Lapidia apicifolia is a loosely ramified shrub 2–4 m high, stem tomentose, leaves opposite-decussate, laminae conduplicate, petiolate, receptacle flat, epaleaceous, glabrous, and pappus of bristles fused at base, irregular in length, fringed and purplish. In a phylogenetic analysis using sequence data from ITS and trnL-trnF of selected members of Eupatorieae, Lapidia is indicated as sister group of a highly supported clade with Morithamnus, Bahianthus and Catolesia. This group is composed by loosely branched shrubs, most with leaves that are lax, stems, leaves and involucral bracts that are viscid (Bahianthus and Morithamnus) and, if not, trichomes (tomentose indumentum) are developed (Lapidia), to protect against both solar radiation and loss of water. A description, illustrations, and a discussion about related and sympatric genera are presented.


Phytotaxa ◽  
2021 ◽  
Vol 509 (3) ◽  
Author(s):  
KUNHIRAMAN C. RAJESHKUMAR ◽  
RAJNISH K. VERMA ◽  
SARANYAPHAT BOONMEE ◽  
SAJINI CHANDRASIRI ◽  
KEVIN D. HYDE ◽  
...  

This study introduces, Paradictyocheirospora, a new genus, belonging to the family Dictyosporiaceae with evidence from morphology and phylogenetic analysis of a combined ITS, LSU and TEF1α sequence data. Paradictyocheirospora can be distinguished from allied taxa based on dictyosporous, cheiroid conidia with suprabasal bubble-like hyaline appendages and cylindrical, reduced conidiophores. The new genus is similar to Dictyocheirospora; however, the conidia are smaller with fewer rows (3–6) and have globose, supra-basal appendages. Paradictyocheirospora tectonae forms a distinct lineage basal to Digitodesmium and Dictyocheirospora. Additional new collections from Thane, Maharashtra were morphologically identical and phylogenetically related to Dictyocheirospora nabanheensis, and the latter is a new record from India.


2019 ◽  
Vol 165 (1) ◽  
pp. 21-31 ◽  
Author(s):  
A. M. Dullemans ◽  
M. Botermans ◽  
M. J. D. de Kock ◽  
C. E. de Krom ◽  
T. A. J. van der Lee ◽  
...  

Abstract To obtain insight into the sequence diversity of strawberry latent ringspot virus (SLRSV), isolates from collections and diagnostic samples were sequenced by high-throughput sequencing. For five SLRSV isolates, the complete genome sequences were determined, and for 18 other isolates nearly complete genome sequences were determined. The sequence data were analysed in relation to sequences of SLRSV and related virus isolates available in the NCBI GenBank database. The genome sequences were annotated, and sequences of the protease-polymerase (Pro-Pol) region and coat proteins (CPs) (large and small CP together) were used for phylogenetic analysis. The amino acid sequences of the Pro-Pol region were very similar, whereas the nucleotide sequences of this region were more variable. The amino acid sequences of the CPs were less similar, which was corroborated by the results of a serological comparison performed using antisera raised against different isolates of SLRSV. Based on these results, we propose that SLRSV and related unassigned viruses be assigned to a new genus within the family Secoviridae, named “Stralarivirus”. Based on the phylogenetic analysis, this genus should include at least three viruses, i.e., SLRSV-A, SLRSV-B and lychnis mottle virus. The newly generated sequence data provide a basis for designing molecular tests to screen for SLRSV.


2013 ◽  
Vol 63 (Pt_5) ◽  
pp. 1912-1921 ◽  
Author(s):  
Xumiao Chen ◽  
Ying Yan ◽  
Xiaozhong Hu ◽  
Mingzhuang Zhu ◽  
Honggang Ma ◽  
...  

The morphology and morphogenesis of the stylonychine hypotrich Rigidohymena candens (Kahl, 1932) Berger, 2011, isolated from garden soil in Qingdao, China, were investigated using live observation and protargol impregnation methods. The Qingdao isolate possesses all diagnostic morphological characters of R. candens. The main events during binary fission are as follows: (i) the proter retains the parental adoral zone of membranelles entirely, whereas the old undulating membranes dedifferentiate into an anlage that gives rise to the leftmost frontal cirrus and the new undulating membranes of the proter; (ii) five streaks of fronto-ventral-transverse cirral anlagen are segmented in the pattern 3 : 3 : 3 : 4 : 4 from left to right, which form two frontal, four frontoventral, one buccal, five ventral and five transverse cirri, respectively; (iii) dorsal morphogenesis is in the typical Oxytricha pattern; (iv) three caudal cirri are formed, one at the posterior end of each of dorsal kineties 1, 2 and 4; and (v) the postoral ventral cirrus V/3 is not involved in primordia formation. The morphological and morphogenetic observations and phylogenetic analyses based on the small-subunit rDNA sequence data support the validity of Rigidohymena Berger, 2011 and its systematic position in the subfamily Stylonychinae.


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