Morphological and molecular characterisation of a new awl nematode, Dolichodorus mediterraneus sp. n. (Nematoda: Dolichodoridae), from Spain

Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Blanca Landa ◽  
Gracia Liébanas ◽  
Pablo Castillo ◽  
Raquel Murillo Navarro ◽  
Domingo Jiménez Guirado
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Large Subunit ◽  
First Record ◽  
Molecular Data ◽  
Quercus Suber ◽  
The Body ◽  
Southern Spain ◽  
Scanning Electron

AbstractA new species of awl nematode, Dolichodorus mediterraneus sp. n., is described from southern Spain. The new species is described and illustrated by light microscopy and scanning electron microscopy studies from specimens collected in a sandy soil around roots of cork oak (Quercus suber L.) from Hato Raton, Aznalcázar (Seville), southern Spain. Dolichodorus mediterraneus sp. n. is characterised by a prominent, rounded, lip region, which is offset from the body and has four to six very fine annuli; lateral fields areolated with three lines, ending posterior to anus level; tail 72-122 μm long, abruptly tapering to an acuminate, often spicate, terminus; tail projection 51-79 μ m long; spicules ventrally arcuate, 33-42 μm long; gubernaculum slightly ventrally curved, 14-22 μm long. Scanning electron microscopy observations showed a similar en face pattern to other Dolichodorus spp. Molecular data from the ribosomal large subunit D2-D3 expansion segment of 28S rDNA from paratypes yielded a single fragment of approximately 800 bp. This is the first record of the genus Dolichodorus in Spain and only the second in Europe. A key to species is provided.

scholarly journals Balantidium grimi n. sp. (Ciliophora, Litostomatea), a new species inhabiting the rectum of the frog Quasipaa spinosa from Lishui, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 29 ◽  
Author(s):  
Weishan Zhao ◽  
Can Li ◽  
Dong Zhang ◽  
Runqiu Wang ◽  
Yingzhen Zheng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
New Species ◽  
Body Shape ◽  
Ssu Rdna ◽  
The Body ◽  
Molecular Phylogenetic ◽  
A New Species ◽  
Scanning Electron

Balantidium grimi n. sp. is described from the rectum of the frog Quasipaa spinosa (Amphibia, Dicroglossidae) from Lishui, Zhejiang Province, China. The new species is described by both light microscopy (LM) and scanning electron microscopy (SEM), and a molecular phylogenetic analysis is also presented. This species has unique morphological features in that the body shape is somewhat flattened and the vestibulum is “V”-shaped, occupying nearly 3/8 to 4/7 of the body length. Only one contractile vacuole, situated at the posterior body, was observed. The phylogenetic analysis based on SSU-rDNA indicates that B. grimi groups together with B. duodeni and B. entozoon. In addition, the genus Balantidium is clearly polyphyletic.

scholarly journals An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

2016 ◽  
Author(s):  
Lucio Bonato ◽  
Marzio Zapparoli ◽  
Leandro Drago ◽  
Alessandro Minelli
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Body Surface ◽  
Sensu Stricto ◽  
The Body ◽  
European Species ◽  
Scanning Electron ◽  
Trunk Segments

Endogeophilus ichnusae gen. et sp. nov. (Chilopoda: Geophilidae sensu stricto) is described based on three specimens from two localities in south-western Sardinia, examined by light and scanning electron microscopy. The new centipede resembles the rare Ibero-Pyrenean genus Galliophilus Ribaut & Brolemann, 1927 in some features, especially in the forcipular segment, and the temperate European species Geophilus electricus (Linnaeus, 1758) in other features, especially in the ultimate leg-bearing segment. However, the true affinities of E. ichnusae gen. et sp. nov. are uncertain, because the new species departs significantly from the majority of geophilids for the higher number of legs (91–107 pairs in the specimens examined), the slender trunk segments (the sternites being longer than wide), the relatively stout legs (the tarsus being only about twice as long as wide) and the very short setae (≤ 15 mm) scattered on the body surface. All these features are probably derived and suggest adaptation to a more strictly endogeic habit than other geophilids.

scholarly journals A new species of Cosmocerca (Nematoda, Ascaridomorpha) from the marine toad Rhinella marina (Linnaeus) (Anura, Bufonidae) in Australia

ZooKeys ◽  
2020 ◽  
Vol 931 ◽  
pp. 11-20
Author(s):  
Xue-Feng Ni ◽  
Diane P. Barton ◽  
Hui-Xia Chen ◽  
Liang Li
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Present Knowledge ◽  
The Body ◽  
Amphibian Species ◽  
Rhinella Marina ◽  
A New Species ◽  
Scanning Electron ◽  
Nematode Fauna

The marine toad Rhinella marina (Linnaeus) (Anura, Bufonidae) is a notorious, exotic amphibian species in Australia. However, our present knowledge of the composition of the nematode fauna of R. marina is still not complete. In the present study, a new cosmocercid nematode, Cosmocerca multipapillatasp. nov., was described using both light and scanning electron microscopy, based on specimens collected from R. marina in Australia. Cosmocerca multipapillatasp. nov. can be easily distinguished from its congeners by the body size, the presence of lateral alae and well sclerotized gubernaculum, the number and arrangement of plectanes and rosettes and the length of spicules, oesophagus and tail.

Freshwater Bryozoa of Okinawa, Japan, with descriptions of Rumarcanella gen. nov. (Phylactolaemata: Plumatellidae) and two new species

Zootaxa ◽  
2011 ◽  
Vol 2732 (1) ◽  
pp. 1 ◽  
Author(s):  
MASATO HIROSE ◽  
SHUNSUKE F. MAWATARI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
New Genus ◽  
First Record ◽  
Molecular Study ◽  
Okinawa Island ◽  
Two New Species ◽  
Freshwater Bryozoa ◽  
Scanning Electron

We identified nine species of phylactolaemate bryozoans collected from ponds and dams on Okinawa Island, Japan. This is the first report on freshwater bryozoans from Okinawa and includes the first record of Plumatella javanica from Japan. Phylactolaemate species were identified on the basis of colony and statoblast morphology and are illustrated by light microscopy and scanning electron microscopy (SEM). We detected two new plumatellid species that, like Plumatella minuta and Plumatella vorstmani, have a transparent, weakly chitinized colony and hypertubercles on the floatoblast fenestra. A previous molecular study showed these four species to form a clade separate from Plumatella and Hyalinella. We here establish the new genus Rumarcanella to accommodate these species and describe Rumarcanella gusuku n. sp. and R. yanbaruensis n. sp. We discuss the dispersal of bryozoans to and from Japan by birds carrying statoblasts and provide a key to the Phylactolaemata of Japan.

Nitzschia papillosa (Bacillariaceae, Bacillariophyta): a new species from a Brazilian salt marsh

Phytotaxa ◽  
2016 ◽  
Vol 247 (1) ◽  
pp. 75 ◽  
Author(s):  
Lucielle Merlym Bertolli ◽  
Dávia Marciana Talgatti ◽  
Lezilda Carvalho Torgan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Salt Marsh ◽  
First Record ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Patos Lagoon ◽  
A New Species ◽  
Scanning Electron

A new species of the genus Nitzschia was found in epiphyton and microphytobenthos at the Patos Lagoon salt marsh (Rio Grande do Sul State, southern Brazil). Nitzschia papillosa sp. nov. was described under light and scanning electron microscopy and compared with other species from the section Lanceolatae. The species presents features shared by several species of the genus such as a linear-lanceolate outline, cuneate ends and irregularly distributed fibulae. The distinguishable feature is a row of dots on the margin opposite to the fibulae. In scanning electron microscopy, these structures appear as silica excrescences, little rounded or squared, which we refer as papillae, situated at the transapical costae in the internal valve face. This is the first record of this kind of ornamentation in Nitzschia species.

scholarly journals Ecotone shifts in southern Madagascar: first barcoding data and six new species of the endemic millipede genus Riotintobolus (Spirobolida, Pachybolidae)

ZooKeys ◽  
2020 ◽  
Vol 953 ◽  
pp. 1-29
Author(s):  
Thomas Wesener
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Genetic Data ◽  
Molecular Data ◽  
Additional Species ◽  
Critically Endangered Species ◽  
Littoral Forest ◽  
Genetic Barcoding ◽  
Scanning Electron

Six new species of the Spirobolida millipede genus Riotintobolus Wesener, 2009, are described from the spiny forest in southern Madagascar utilising genetic barcoding, drawings and scanning electron microscopy: Riotintobolus tsimelahysp. nov., R. mangatsiakasp. nov., R. lavanonosp. nov., R. bovinussp. nov., R. antafokysp. nov. and R. makayisp. nov. One other Riotintobolus population from the spiny forest might represent an additional species based on genetic data, but it cannot be described as no male specimens were collected. At present, the genus Riotintobolus Wesener, 2009 has eight species from the spiny forest and two species from the littoral rainforest. A determination key to all ten species of the genus is provided. Molecular data reveal that the two critically endangered species from the humid littoral rainforest are not closely related to one another, but have their closest relative in the dry spiny forest ecosystem. Riotintobolus mandenensis Wesener, 2009, only known from the southern littoral rainforest of Mandena is related to R. tsimelahysp. nov. from the nearby spiny forest at Tsimelahy with a p-distance of 11%, while R. minutus Wesener, 2009 from the littoral forest of Sainte Luce is more distant to all other Riotintobolus species, but more closely related to R. bovinussp. nov. from the southwestern forest of the Makay.

Portanini (Insecta, Hemiptera, Cicadellidae): morphology of female terminalia, first record of host plants, a new species of Portanus from Brazil, and taxonomic notes

Zootaxa ◽  
2020 ◽  
Vol 4802 (3) ◽  
pp. 569-581
Author(s):  
MÁRCIO FELIX ◽  
VICTOR QUINTAS ◽  
JÁDILA SANTOS PRANDO ◽  
GABRIEL MEJDALANI
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Host Plants ◽  
First Record ◽  
Minas Gerais ◽  
Junior Synonym ◽  
Sem Images ◽  
A New Species ◽  
Scanning Electron

Portanus adenomari sp. nov. from the Brazilian states of Minas Gerais, Paraná, and Maranhão is described and illustrated. It can be easily distinguished by the aedeagus with a pair of apical, slender curved processes, crossing each other medially. The previously unknown females of Portanus restingalis Felix & Mejdalani, 2016 and Metacephalus facetus (Kramer, 1961), as well as the female terminalia of Metacephalus sakakibarai Souza, Takiya & Felix, 2017, are also described in detail. Scanning electron microscopy (SEM) images of the ovipositor valvulae of P. restingalis are given. Comparative notes on the morphology of the female terminalia in the tribe Portanini are provided. Specimens of P. restingalis were observed feeding and resting on Bromeliaceae leaves; this is the first record of host plants for the Portanini. Metacephalus marica (Felix & Mejdalani, 2016) is regarded as a junior synonym of Metacephalus longicornis (Osborn, 1923). 

Five Nolanea spp. nov. from Brazil

Mycotaxon ◽  
2020 ◽  
Vol 135 (3) ◽  
pp. 589-612
Author(s):  
Fernanda Karstedt ◽  
Sarah E. Bergemann ◽  
Marina Capelari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Rna Polymerase ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sao Paulo ◽  
Metropolitan Region ◽  
Scanning Electron

Five new species of Nolanea (Entolomataceae, Agaricales)—N. albertinae, N. atropapillata, N. pallidosalmonea, N. parvispora, and N. tricholomatoidea—were collected from the São Paulo metropolitan region. Phylogenetic analyses based on partial sequences of the mitochondrial small subunit (mtSSU), nuclear large subunit (nLSU) and second largest RNA polymerase subunit (rpb2), confirm that all these species belong to Nolanea. Morphological descriptions, comments, illustrations, scanning electron microscopy and color photos are included.

scholarly journals Three new species of Pleurothallis (Orchidaceae: Pleurothallidinae) in subsection Macrophyllae-Fasciculatae from northern South America

Lankesteriana ◽  
2016 ◽  
Vol 16 (3) ◽  
Author(s):  
Mark Wilson ◽  
Luis Baquero ◽  
Katharine Dupree ◽  
Marco M. Jiménez ◽  
Cheryl M. LeBlanc ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
South America ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
History Of ◽  
Northern South America ◽  
Three New Species ◽  
Scanning Electron

The history of the taxonomy of Pleurothallis R.Br. subsection Macrophyllae-Fasciculatae and recent descriptions in that group are summarized. The phylogenetic position of the group based on preliminary molecular data and the appropriateness of the proposed genera Acronia C.Presl. and Zosterophyllanthos Szlach. & Marg. for this group are discussed. Three new species from northern South America are described: Pleurothallis rubrifolia from southeastern Ecuador and northeastern Peru; Pleurothallis nangaritzae from southeastern Ecuador; and Pleurothallis castanea. Labellar micromorphology examined by scanning electron microscopy for P. rubrifolia and P. nangaritzae is discussed in relation to taxonomy and possible pollinator interactions.

A new species of alcyonacean octocoral from the Peruvian zoogeographic region

2014 ◽  
Vol 94 (3) ◽  
pp. 493-498 ◽  
Author(s):  
Odalisca Breedy ◽  
Hector M. Guzman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Species Richness ◽  
New Species ◽  
Light Microscopy ◽  
First Record ◽  
Shallow Waters ◽  
Morphological Studies ◽  
A New Species ◽  
Scanning Electron

The first record of the plexaurid genus Psammogorgia from the shallow waters of Peru is reported here. A new species (Psammogorgia hookeri sp. nov.) is described from Isla San Gallán, Paracas National Reserve. The new species was morphologically analysed using light microscopy and scanning electron microscopy. It is distinguished from the others in the genus by its small size; prominent calyces with wide lips around polyp apertures and without a special type of sclerites, but with a concentration of irregular, thorny spindles and wart-clubs around the calyx rim; coenenchymal sclerites that do not reach more than 0.2 mm long; the occurrence of conspicuous star-like radiates; and the colour of the colony and coenenchymal sclerites, which is coral red. The results increase species richness within a genus that has not been studied for more than a century, and contribute to the establishment of characters for further morphological studies. Additionally, the new species adds value to existing protected areas and to the octocoral biodiversity records for the Peruvian region and the eastern Pacific.

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