Five Nolanea spp. nov. from Brazil

Mycotaxon ◽  
2020 ◽  
Vol 135 (3) ◽  
pp. 589-612
Author(s):  
Fernanda Karstedt ◽  
Sarah E. Bergemann ◽  
Marina Capelari
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Rna Polymerase ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Small Subunit ◽  
Sao Paulo ◽  
Metropolitan Region ◽  
Scanning Electron

Five new species of Nolanea (Entolomataceae, Agaricales)—N. albertinae, N. atropapillata, N. pallidosalmonea, N. parvispora, and N. tricholomatoidea—were collected from the São Paulo metropolitan region. Phylogenetic analyses based on partial sequences of the mitochondrial small subunit (mtSSU), nuclear large subunit (nLSU) and second largest RNA polymerase subunit (rpb2), confirm that all these species belong to Nolanea. Morphological descriptions, comments, illustrations, scanning electron microscopy and color photos are included.

scholarly journals Unveiling the Hidden Diversity of Rock-Inhabiting Fungi: Chaetothyriales from China

2020 ◽  
Vol 6 (4) ◽  
pp. 187
Author(s):  
Wei Sun ◽  
Lei Su ◽  
Shun Yang ◽  
Jingzu Sun ◽  
Bingjie Liu ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Small Subunit ◽  
Internal Transcribed Spacer Region ◽  
Natural Rock ◽  
Rock Inhabiting Fungi

Rock-inhabiting fungi (RIF) are nonlichenized fungi that naturally colonize rock surfaces and subsurfaces. The extremely slow growth rate and lack of distinguishing morphological characteristics of RIF resulted in a poor understanding on their biodiversity. In this study, we surveyed RIF colonizing historical stone monuments and natural rock formations from throughout China. Among over 1000 isolates, after preliminary delimitation using the internal transcribed spacer region (ITS) sequences, representative isolates belonging to Trichomeriaceae and Herpotrichiellaceae were selected for a combined analysis of ITS and the nuclear ribosomal large subunit (nucLSU) to determine the generic placements. Eight clades representing seven known genera and one new genus herein named as Anthracina were placed in Trichomeriaceae. While, for Herpotrichiellaceae, two clades corresponded to two genera: Cladophialophora and Exophiala. Fine-scale phylogenetic analyses using combined sequences of the partial actin gene (ACT), ITS, mitochondrial small subunit ribosomal DNA (mtSSU), nucLSU, the largest subunit of RNA polymerase II (RPB1), small subunit of nuclear ribosomal RNA gene (SSU), translation elongation factor (TEF), and β-tubulin gene (TUB) revealed that these strains represented 11 and 6 new species, respectively, in Trichomeriaceae and Herpotrichiellaceae. The 17 new species were described, illustrated for their morphologies and compared with similar taxa. Our study demonstrated that the diversity of RIF is surprisingly high and still poorly understood. In addition, a rapid strategy for classifying RIF was proposed to determine the generic and familial placements through preliminary ITS and nucLSU analyses, followed by combined analyses of five loci selected from ACT, ITS, mtSSU, nucLSU, RPB1, and/or the second subunit of RNA polymerase II gene (RPB2), SSU, TEF, and TUB regions to classify RIF to the species level.

Taxonomy and ecology of Fragilaria billingsii sp. nov. and analysis of type material of Synedra rumpens var. fusa (Fragilariaceae, Bacillariophyta) from Brazil

Phytotaxa ◽  
2016 ◽  
Vol 270 (3) ◽  
pp. 191 ◽  
Author(s):  
SIMONE WENGRAT ◽  
EDUARDO A. MORALES ◽  
CARLOS E. WETZEL ◽  
PRYSCILLA D. ALMEIDA ◽  
LUC ECTOR ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Sao Paulo ◽  
The State ◽  
Type Material ◽  
Literature Analysis ◽  
Ecological Preferences ◽  
Multipurpose Reservoir ◽  
Scanning Electron

Synedra rumpens var. fusa was originally described by Patrick, but there are only a few subsequent records of this variety in the literature. Analysis of samples from Brazilian reservoirs containing a morphologically similar taxon led us to the analysis of the type material of S. rumpens var. fusa, performed using light (LM) and scanning electron microscopy (SEM). In the present manuscript, an amendment to the description of the var. fusa is made and its transfer to the genus Fragilaria as F. fusa comb. et stat. nov. is proposed. Moreover, Fragilaria billingsii sp. nov. is described from a large multipurpose reservoir from the State of São Paulo, Brazil. Comparative discussions with morphologically related taxa found in the literature are presented. Regarding ecological preferences, the new species was found living in slightly acidic and meso-eutrophic waters.

Morphological and molecular characterisation of a new awl nematode, Dolichodorus mediterraneus sp. n. (Nematoda: Dolichodoridae), from Spain

Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Blanca Landa ◽  
Gracia Liébanas ◽  
Pablo Castillo ◽  
Raquel Murillo Navarro ◽  
Domingo Jiménez Guirado
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Large Subunit ◽  
First Record ◽  
Molecular Data ◽  
Quercus Suber ◽  
The Body ◽  
Southern Spain ◽  
Scanning Electron

AbstractA new species of awl nematode, Dolichodorus mediterraneus sp. n., is described from southern Spain. The new species is described and illustrated by light microscopy and scanning electron microscopy studies from specimens collected in a sandy soil around roots of cork oak (Quercus suber L.) from Hato Raton, Aznalcázar (Seville), southern Spain. Dolichodorus mediterraneus sp. n. is characterised by a prominent, rounded, lip region, which is offset from the body and has four to six very fine annuli; lateral fields areolated with three lines, ending posterior to anus level; tail 72-122 μm long, abruptly tapering to an acuminate, often spicate, terminus; tail projection 51-79 μ m long; spicules ventrally arcuate, 33-42 μm long; gubernaculum slightly ventrally curved, 14-22 μm long. Scanning electron microscopy observations showed a similar en face pattern to other Dolichodorus spp. Molecular data from the ribosomal large subunit D2-D3 expansion segment of 28S rDNA from paratypes yielded a single fragment of approximately 800 bp. This is the first record of the genus Dolichodorus in Spain and only the second in Europe. A key to species is provided.

scholarly journals Species Diversity and Molecular Phylogeny of Cyanosporus (Polyporales, Basidiomycota)

2021 ◽  
Vol 12 ◽  
Author(s):  
Shun Liu ◽  
Lu-Lu Shen ◽  
Yan Wang ◽  
Tai-Min Xu ◽  
Genevieve Gates ◽  
...  
Keyword(s):  
New Species ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Brown Rot ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ribosomal Rna Gene

Cyanosporus is a cosmopolitan brown-rot fungal genus, recognizable by blue-tinted basidiocarps. Species in this genus were usually treated as belonging to the Postia caesia complex, however, recent phylogenetic analyses showed that this complex represents an independent genus. During further studies on Cyanosporus, five new species were discovered based on morphological features and molecular data. Phylogenetic analyses of Cyanosporus were conducted using the internal transcribed spacer (ITS) regions, the large subunit of nuclear ribosomal RNA gene (nLSU), the small subunit of nuclear ribosomal RNA gene (nSSU), the small subunit of mitochondrial rRNA gene (mtSSU), the largest subunit of RNA polymerase II (RPB1), the second largest subunit of RNA polymerase II (RPB2), and the translation elongation factor 1-α gene (TEF); illustrated descriptions of the new species are provided. In addition, fifteen species previously belonging to the Postia caesia complex are transferred to Cyanosporus and proposed as new combinations.

A new species of Cribraria (Myxomycetes) from Australia

2018 ◽  
Vol 52 (2) ◽  
pp. 379-385 ◽  
Author(s):  
S. L. Stephenson ◽  
Yu. K. Novozhilov ◽  
P. Wellman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Lemon Yellow ◽  
Moist Chamber ◽  
Light Yellow ◽  
Dark Violet ◽  
The Stability ◽  
A New Species ◽  
Scanning Electron

A new species of Cribraria, described herein as C. bicolor, appeared in moist chamber cultures on samples of the bark of Eucalyptus sp. collected at two localities in Australia. The morphology of representative specimens was examined by light and scanning electron microscopy, and micrographs of relevant morphological details of sporocarps and spores are provided. The species has a number of distinct and unique morphological features, including a glossy bright-violet globose sporotheca and a two-colored long stalk which is bright-red over the lower one-third and light yellow or lemon-yellow over the upper two-thirds. The combination of these characteristics as well as a shallow calyculus which is dark-violet when viewed under a dissecting microscope and bright red in transmitted light when mounted in lactophenol makes C. bicolor a well-defined morphospecies when compared to all other species of Cribraria. The stability of the taxonomic characters of the species was confirmed by an examination of a number of specimens.

Encyonema oblonga (Bacillariophyta, Cymbellaceae), a new species from Shanxi Province, China

Phytotaxa ◽  
2021 ◽  
Vol 480 (3) ◽  
pp. 284-290
Author(s):  
QI LIU ◽  
JIAJIA LI ◽  
QUANXI WANG ◽  
JOHN PATRICK KOCIOLEK Patrick Kociolek ◽  
Shulian Xie
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Shanxi Province ◽  
Diatom Flora ◽  
A New Species ◽  
Scanning Electron ◽  
Freshwater Diatom

A new species, Encyonema oblonga Liu & Xie, is collected during a survey of the freshwater diatom flora of Manghe River, Shanxi, China. The valves of this new species are oblong with rounded apices and have the features typical of Encyonema, including complex areolae. We compare the new species with the most similar taxa, E. leei, E. leei var. sinensis and E. appalachianum and consider that  E. leei var. sinensis is the valid name.  The morphology of E. oblonga is documented with light and scanning electron microscopy.

Phylogenetic and morphological analyses of species of the Entolomataceae (Agaricales, Basidiomycota) with cuboid basidiospores

Phytotaxa ◽  
2019 ◽  
Vol 391 (1) ◽  
pp. 1
Author(s):  
FERNANDA KARSTEDT ◽  
MARINA CAPELARI ◽  
TIMOTHY J. BARONI ◽  
DAVID L. LARGENT ◽  
SARAH E. BERGEMANN
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analyses ◽  
Profile View ◽  
Pileus Surface ◽  
Scanning Electron

The generic or subgeneric delimitation by morphology of the Entolomataceae (Agaricales, Basidiomycota) is often based on the habit and external features of the basidiomata, the hyphal arrangement of the pileus surface and the shape of the basidiospores, which possess either bumps or undulate-pustules forming short ridges, or longitudinal ridges or are obviously angular with four to nine angles in profile view. Here, we examine the basidiospore shape of species in the /Entoloma clade described as cuboid to evaluate its importance in taxonomy using both phylogenetic and detailed analyses of the shape with Scanning Electron Microscopy. Our phylogenetic analyses support the placement of species with cuboid basidiospores into one of two clades. Based on this separation, two new subgenera of Entoloma are proposed: Cuboeccilia with an omphalinoid habit and fusoid cystidia and Cubospora which has a mycenoid to tricholomatoid habit and clavate, rarely fusoid cheilocystidia.

Sellaphora pelagonica (Bacillariophyceae), a new species from dystrophic ponds in the Republic of North Macedonia

Phytotaxa ◽  
2021 ◽  
Vol 496 (2) ◽  
pp. 121-133
Author(s):  
HRISTINA KOCHOSKA ◽  
DUŠICA ZAOVA ◽  
ANASTASIJA VIDESKA ◽  
DANIJELA MITIC-KOPANJA ◽  
HRISTINA NAUMOVSKA ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
The Republic ◽  
A New Species ◽  
Scanning Electron ◽  
Intermittent Ponds

Lowland intermittent ponds, although biologically important, are poorly studied water habitats. During the analyses of the material taken from lowland pond in Pelagonia Valley, and Vitachevo, Republic of North Macedonia, the diatom Sellaphora pelagonica sp. nov. was recorded and described based on light and scanning electron microscopy. The species is characterized by a strictly linear valve outline with parallel to slightly concave margins and broadly rounded ends, 15.0–40.0 µm long and 5.0–7.0 µm wide. These features, together with raphe structure and areolar shape are compared with several similar taxa currently ascribed to Sellaphora.

The genus Navicula (Bacillariophyceae, Naviculaceae) from the valley of the Adegoy River (Krasnodar Territory, Russia) and description of two new species

Phytotaxa ◽  
2021 ◽  
Vol 494 (2) ◽  
pp. 208-218
Author(s):  
DMITRY CHUDAEV ◽  
INGRID JÜTTNER ◽  
ZLATKO LEVKOV
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
River Valley ◽  
Similar Species ◽  
Taxonomic Study ◽  
The Caucasus ◽  
Caucasus Region ◽  
Two New Species ◽  
Scanning Electron

This is the first detailed taxonomic study of the genus Navicula in the Krasnodar Territory of the Caucasus region, Russia. During the study of the genus in waterbodies of the Adegoy River valley 15 taxa were recorded. Two species, N. adegoyensis sp. nov. and N. pseudocryptofallax sp. nov., are described as new to science. Their morphology is studied by light and scanning electron microscopy, and comparisons with morphologically similar species are provided. The use of the name N. avenacea for N. lanceolata sensu auct. is discussed. Navicula diversity was higher in the Adegoy River than in pools elsewhere in the river valley.

Fragilaria irregularis sp. nov. a new araphid species (Fragilariaceae, Bacillariophyta) from the River Adegoy, Krasnodar Territory, Russia

Phytotaxa ◽  
2021 ◽  
Vol 508 (2) ◽  
Author(s):  
DMITRY CHUDAEV ◽  
INGRID JÜTTNER ◽  
BART VAN DE VIJVER
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Similar Species ◽  
New Taxon ◽  
Weakly Convex ◽  
Diatom Flora ◽  
A New Species ◽  
Scanning Electron

During a survey of the diatom flora of the Adegoy River (Krasnodar Territory, Russia), an unknown Fragilaria species (Bacillariophyta) was found that could not be identified using the currently available literature and here we describe it as a new species. The morphology of Fragilaria irregularis sp. nov. was studied using light and scanning electron microscopy and compared to similar species including Fragilaria deformis, F. rinoi, F. septentrionalis and Fragilariforma bicapitata. It differed from these taxa in several characters. Most observed valves showed an irregular striation pattern, a feature not observed in other species. Other important differences include valve outline (linear with parallel margins) and stria density (7–14 in 10 µm, lower compared to all other species). The new taxon has a linear valve outline with parallel to weakly convex margins, subcapitate apices and a very irregular, coarse striation pattern.

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