Two new species of Schistonchus (Nematoda: Aphelenchoididae) associated with Ficus hispida in China

Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 169-187 ◽  
Author(s):  
Weimin Ye ◽  
Yongsan Zeng ◽  
Robin Giblin-Davis
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Large Subunit ◽  
Recent Common Ancestor ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Most Recent Common Ancestor ◽  
Ficus Hispida ◽  
Tail Tip

AbstractTwo new nematode species of the genus Schistonchus were recovered from syconia from a single Ficus hispida caprifig tree in Guangzhou, China. They are described herein as Schistonchus guangzhouensis n. sp. and S. centerae n. sp. Schistonchus guangzhouensis n. sp. is characterised by possessing the longest postuterine sac (PUS) of all currently described females in the genus (84-148 μm or >3.5 vulval body diam. (VBD) long), excretory pore situated near the level of the metacorpus, two pairs of subventral papillae on the male tail, and unique recurved and mitten-shaped spicules. Schistonchus centerae n. sp. is characterised by a unique fusiform tail tip and short PUS (8-20 μm or <1.0 VBD long) in reproductive females, excretory pore located near the head, spicules with an indistinct rostrum, male tail with three pairs of papillae, and broadly truncate tail tip. Both new species were easily differentiated from each other and other members of the genus for which sequences of the D2/D3 expansion segments of the large subunit rRNA gene (LSU) and partial small subunit rRNA gene (SSU) were available. Phylogenetic analysis also supported a monophyletic Schistonchus within a well-supported clade of Aphelenchoididae (sensu Hunt, 1993) and shared a most recent common ancestor with Aphelenchoides and Laimaphelenchus.

Description of Schistonchus microcarpus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus microcarpa in China

Nematology ◽  
2011 ◽  
Vol 13 (2) ◽  
pp. 221-233 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin Giblin-Davis ◽  
Changhui Li ◽  
Shinian Zhang ◽  
...  
Keyword(s):  
Excretory Pore ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Mtdna Coi ◽  
Ficus Microcarpa ◽  
Tail Tip

AbstractSchistonchus microcarpus n. sp. was recovered from the syconia of Ficus microcarpa from Shenzhen and Guangzhou, Guangdong Province, China, during a survey of nematode biodiversity from 2007 to 2009. It is characterised by possessing the combined characters of a short post-uterine sac (PUS) (3-11 μm or <0.4 vulval body diam. (VBD) long), excretory pore located just posterior to the head but anterior to the conus level of the stylet, prominent amphids, three pairs of subventral papillae on the male tail (one pair adcloacal, one pair halfway between cloaca and tail terminus, and one pair near tail tip), unique recurved and sickleshaped spicules with finely rounded tip with cucullus, amoeboid sperm, and rounded male tail tip with or without mucron. Schistonchus microcarpus n. sp. is morphologically differentiated from all other described species in this genus by the possession of a spicule with a cucullus on the tip. Schistonchus microcarpus n. sp. was easily differentiated from other sequenced species by the partial small subunit rRNA gene (SSU) and D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with partial SSU sequences suggests that S. microcarpus n. sp. is in a highly supported monophyletic clade with sequenced Schistonchus species except for S. hirtus. Based upon inferences using D3 LSU sequence data, it forms a clade with an undescribed species of Schistonchus ex F. benjamini from Australia and is part of a larger clade of Schistonchus that mostly share the character of an anteriorly placed excretory pore. Sequences of partial mtDNA COI (590 bp) from males of S. microcarpus n. sp. with and without a mucronate tail tip were identical, proving that these two morphotypes are conspecific.

Ficophagus auriculatae n. sp. (Nematoda: Aphelenchoididae), an associate of the Ficus auriculata-complex in China

Nematology ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. 497-508
Author(s):  
Yongsan Zeng ◽  
Jianfeng Huang ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Rongrong Shi ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Small Subunit Rrna Gene ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus auriculata-complex, including F. auriculata, F. oligodon and F. hainanensis, from Xishuangbanna Tropical Botanical Garden, China. It is described herein as Ficophagus auriculatae n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located between the metacorpus and the stylet basal knobs, testis multi-reflexed, amoeboid sperm, three pairs of subventral papillae on male tail, rounded male tail tip without mucron, absence of gubernaculum (= apophysis) and sickle-shaped spicules with terminal cucullus. Ficophagus auriculatae n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analysis with the above-mentioned sequences suggested that F. auriculatae n. sp. has a closer relationship with F. fleckeri in the same highly supported monophyletic clade than with the other sequenced Ficophagus and Schistonchus sensu lato species.

Schistonchus fistulosus n. sp. (Nematoda: Aphelenchoididae) associated with Ficus fistulosa in China

Nematology ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 879-890 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Xinrong Wang ◽  
Zhijian Du ◽  
Robin M. Giblin-Davis
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Tail Tip

A new nematode species was recovered from syconia of Ficus fistulosa from the South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong Province, China, during a survey of nematode biodiversity from 2007 to 2012. It is described herein as Schistonchus fistulosus n. sp. and is typologically characterised by possessing the combined characters of a long post-uterine sac, excretory pore located near the metacorpus, amoeboid sperm, three pairs of subventral papillae on the male tail, a mucron on the male tail tip, absence of a gubernaculum and possession of ox-horn-shaped spicules without a terminal cucullus and with a short, broad and bluntly rounded rostrum. Schistonchus fistulosus n. sp. was easily differentiated from other sequenced species by the partial small subunit rRNA gene (SSU) and D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with partial SSU sequences suggests that S. fistulosus n. sp. has a closer relationship with S. guangzhouensis and S. baculum in the same highly supported monophyletic clade than to the other sequenced Schistonchus species. Based upon inferences using LSU D3 sequence data, it is also in a well-supported monophyletic clade with S. guangzhouensis and S. baculum.

Ficophagus curtipes n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus curtipes in China

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1091-1101
Author(s):  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
Yanqiong Peng ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Male Tail ◽  
Tail Tip ◽  
Large Subunit Rrna

Summary A new nematode species was recovered from the syconia of Ficus curtipes from Xishuangbanna Tropical Botanical Garden, Yunnan, China. It is described herein as Ficophagus curtipes n. sp. and is differentiated from all currently described species in the genus by the possession of a spicule with a ventrally recurved tip (terminal barb). It is further characterised by possessing a short post-uterine sac, an ovoid spermatheca, presence of crustaformeria, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip without mucron, rose-thorn-shaped spicules without cucullus and absence of gubernaculum (= apophysis). Ficophagus curtipes n. sp. was differentiated from other sequenced species by the partial small subunit rRNA gene and D2-D3 expansion segments of the large subunit rRNA gene. Phylogenetic analyses suggested that F. curtipes n. sp. has a closer relationship with F. benjamina and F. microcarpus in the same highly supported monophyletic clade than to the other sequenced Ficophagus and Schistonchus s.l. species.

Ficophagus chaozhouensis n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta Vahl var. roxburghii (Miq.) King in China

Nematology ◽  
2020 ◽  
Vol 22 (3) ◽  
pp. 299-312
Author(s):  
Wensheng Zeng ◽  
Dayuan Zhang ◽  
Jianghua Huang ◽  
Yongsan Zeng ◽  
Weimin Ye ◽  
...  
Keyword(s):  
Large Subunit ◽  
Nematode Species ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Monophyletic Clade ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Ficus Hirta ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus hirta var. roxburghii from Chaozhou, Guangdong, China. It is described herein as Ficophagus chaozhouensis n. sp. and is characterised by possessing the combined characters of a short post-uterine sac, excretory pore located near the head, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron (occasionally swollen), absence of gubernaculum (or apophysis), a blunt rosethorn-shaped spicule without a terminal cucullus, and a digitate rostrum with a broadly squared tip. Ficophagus chaozhouensis n. sp. was separated from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that F. chaozhouensis n. sp. is clustered in the same highly supported monophyletic clade with F. aculeata, F. maxima and F. yoponensis, and is sister to F. aculeata.

Martininema semicordatae n. sp. (Nematoda: Aphelenchoididae) associated with Ficus semicordata in China

Nematology ◽  
2020 ◽  
Vol 22 (7) ◽  
pp. 759-770
Author(s):  
Xiulan Zhao ◽  
Dayuan Zhang ◽  
Wensheng Zeng ◽  
Jianfeng Huang ◽  
Yongsan Zeng ◽  
...  
Keyword(s):  
Yunnan Province ◽  
Common Ancestor ◽  
Large Subunit ◽  
Nematode Species ◽  
Botanical Garden ◽  
Small Subunit ◽  
Rrna Gene ◽  
Male Tail ◽  
Lsu Rrna Gene ◽  
Tail Tip

Summary A new nematode species was recovered from the syconia of Ficus semicordata from Xishuangbanna Tropical Botanical Garden, Yunnan Province, China. It is described herein as Martininema semicordatae n. sp. and is characterised by having the combined characters of a long post-vulval uterine sac, excretory pore located at nerve ring level, amoeboid sperm, three pairs of subventral papillae on the male tail, rounded male tail tip with mucron, absence of gubernaculum (= apophysis), and a recurved, rose-thorn-shaped spicule lacking a terminal cucullus. Martininema semicordatae n. sp. differs from other sequenced species by differences in the partial small subunit (SSU) rRNA gene and D2-D3 expansion segments of the large subunit (LSU) rRNA gene. Phylogenetic analysis with LSU D2-D3 expansion segment sequences suggested that M. semicordatae n. sp. is clustered in a highly supported monophyletic clade with M. guangzhouense, M. fistulosus and M. baculum, and shares a common ancestor with these three species.

Angiostomaglandicolasp. n. (Nematoda: Angiostomatidae): a parasite in the land snailMegausteniasp. from the Cat Tien Forest, Vietnam

2009 ◽  
Vol 84 (3) ◽  
pp. 297-304 ◽  
Author(s):  
E.S. Ivanova ◽  
S.E. Spiridonov
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Host Location ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Nerve Ring ◽  
Lsu Rdna ◽  
Dorsal Edge ◽  
Tail Tip ◽  
Specific Number

AbstractThe nematodeAngiostoma glandicolasp. n. found parasitizingMegausteniasp. snails (Pulmonata: Helicarionidae) collected in Dong Nai Province, Vietnam, is described and illustrated. The new species is characterized by its unusual host location (the digestive gland) and in having: two circles of cephalic probolae, six lips each bearing two prominent papillae, amphids situated at posterior of lips, the presence of lateral alae, an enlarged stoma, a club-shaped pharynx with cuticularized valves in the bulb, the nerve ring situated on the junction of the corpus and isthmus and the excretory pore opposite the bulb. Oviparous females have a mid-body vulva position, long reflexed ovaries and short divergent uteri, and a conical tail with a slightly triangular tip. Males have long, curved, not distinctly cephalate spicules and a small gubernaculum, nine pairs of pedunculate genital papillae (GP) incorporated into the bursa (three precloacal, six postcloacal in two groups: GP 4–7 closely behind the cloaca and GP 8–9 distanced), of which GP 5 and 8 terminate on dorsal edge of the bursa and GP 8 and 9 are fused at the base, the bursa nearly reaches the tail tip, unpaired precloacal GP and a pair of ventral circumcloacal GP not incorporated into bursa. It clearly differs from other species of the genus by having cephalic probolae vs. no cephalic probolae and the specific number and disposition of male GP. A partial sequence of D2D3 large subunit (LSU) rDNA was obtained and subjected to phylogenetic analyses. Relationships within theAngiostomagenus are discussed.

Schistonchus hirtus n. sp. (Nematoda: Aphelenchoididae), an associate of Ficus hirta in China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 543-556 ◽  
Author(s):  
Yongsan Zeng ◽  
Weimin Ye ◽  
Robin M. Giblin-Davis ◽  
Changhui Li ◽  
Zhijian Du ◽  
...  
Keyword(s):  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Small Subunit ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Wasp Species ◽  
Dna Sequence Data ◽  
Ficus Hirta ◽  
Large Subunit Rrna

Abstract A nematode recovered from syconia of Ficus hirta from Guangzhou, P. R. China, during a survey of nematode biodiversity from 2007 to 2009, is described herein as Schistonchus hirtus n. sp. and is differentiated by a combination of morphological characters, including excretory pore (EP) located near the metacorpus, a short post-uterine sac (PUS) (0.5 vulval body diam. (VBD) long), rose thorn-shaped spicules, amoeboid sperm, absence of gubernaculum, three pairs of subventral papillae on the male tail, host-Ficus and host-wasp species and DNA sequence data. Morphologically, S. hirtus n. sp. is close to S. centerae, S. altermacrophylla, S. aureus, S. laevigatus and S. virens based upon the length of the PUS (about 0.5 VBD long). However, the relative position of the EP in S. hirtus n. sp. is very different from these species (near metacorpus vs near head). With regard to the EP character, S. hirtus n. sp. is very similar to S. macrophylla, S. guangzhouensis and S. caprifici where the EP is at metacorpus level. However, S. hirtus n. sp. differs from S. macrophylla and S. guangzhouensis by possessing a shorter PUS and smaller spicules, and differs from S. caprifici by a shorter female stylet and smaller spicules. Schistonchus hirtus n. sp. was easily differentiated from other sequenced species by the proportion of parsimony informative changes in the partial small subunit rRNA gene (SSU) and D2/D3 expansion segments of the large subunit rRNA gene (LSU). Phylogenetic analysis with SSU sequences suggests that S. hirtus n. sp. is in a highly supported monophyletic clade with Aphelenchoides and Laimaphelenchus and is polyphyletic to other sequenced Schistonchus species. With LSU sequence data, it forms a clade with S. caprifici and they appear polyphyletic relative to S. guangzhouensis, S. centerae, S. aureus, S. laevigatus and S. virens.

Nematodes from vernal pools in the Santa Rosa Plateau Ecological Reserve, California I. Hirschmanniella santarosae sp. n. (Nematoda: Pratylenchidae), a cryptic sibling species of H. pomponiensis Abdel-Rahman & Maggenti, 1987

Nematology ◽  
2007 ◽  
Vol 9 (3) ◽  
pp. 405-429 ◽  
Author(s):  
Paul De Ley ◽  
Melissa Yoder ◽  
Manuel Mundo-Ocampo ◽  
Irma Tandingan De Ley
Keyword(s):  
New Species ◽  
Sibling Species ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Sibling Relationship ◽  
Gene Trees ◽  
Species Status ◽  
Ecological Reserve ◽  
Tail Tip

AbstractHirschmanniella santarosae sp. n. is described from the largest vernal pool in the Santa Rosa Plateau Ecological Reserve, Murrieta, California, USA. The cryptic new species is morphologically very close to H. pomponiensis and H. gracilis as it can be distinguished from the former only by a more anterior excretory pore position and by more flattened as well as laterally expanded stylet knobs, whilst it differs from the latter in the greater distance from phasmid to tail tip and in the bursal alae ending near the phasmids rather than near the tail tip. Analysis of the rDNA small subunit (SSU) and D2D3 expansion segments of the large subunit (LSU) shows that H. santarosae sp. n. is genetically distinct, having respective sequence homology of 98.89% and 95.9% with H. pomponiensis for these loci. Congruence in SSU and D2D3 gene trees as well as unambiguous character autapomorphies further support the new species status of H. santarosae sp. n. and sibling relationship with H. pomponiensis. Although many more isolates and species will need to be studied before informative biogeographic analyses can be performed, the presently available sequence data suggest that some Hirschmanniella lineages have diversified independently on either side of the Atlantic.

Characterisation of, and entomopathogenic studies on, Pristionchus aerivorus (Cobb in Merrill & Ford, 1916) Chitwood, 1937 (Rhabditida: Diplogastridae) from North Carolina, USA

Nematology ◽  
2015 ◽  
Vol 17 (5) ◽  
pp. 567-580
Author(s):  
Weimin Ye ◽  
Qing Yu ◽  
Natsumi Kanzaki ◽  
Paul R. Adams ◽  
Yasmin J. Cardoza
Keyword(s):  
North Carolina ◽  
Helicoverpa Zea ◽  
Large Subunit ◽  
Small Subunit ◽  
Morphological Observation ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Morphological Studies ◽  
The Status ◽  
Molecular Phylogenetic

During a survey of entomopathogenic nematodes in North Carolina, USA, aPristionchusspecies was recovered using theGalleriabait method. Morphological studies with light microscopy and scanning electron microscopy, mating tests with reference strains, as well as molecular analyses of the near-full-length small subunit rRNA gene (18S) and D2-D3 expansion segments of the large subunit rRNA gene (28S) identified this isolate asPristionchus aerivorus. ExposedGallerialarvae were killed within 48 h and high numbers of nematodes were recovered from the cadavers about 5 days later. Preliminary tests revealed that this nematode is capable of infecting at least two other insect species (Helicoverpa zeaandTenebrio molitor) under laboratory conditions. The status of the genusChroniodiplogasteris discussed and confirmed as a junior synonym ofPristionchusbased on morphological observation and molecular phylogenetic analysis.

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