Pathogenicity of four entomopathogenic nematode strains against Asian corn borer, Ostrinia furnacalis (Guenée), in Vietnam

Nematology ◽  
2018 ◽  
Vol 20 (8) ◽  
pp. 729-736
Author(s):  
Chau N. Nguyen ◽  
Anh T. Do ◽  
Phuc K. Hoang ◽  
Lam X. Truong
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematode ◽  
Reproductive Capacity ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Heterorhabditis Indica ◽  
Low Concentrations ◽  
Asian Corn Borer ◽  
High Virulence ◽  
High Degree

Summary The virulence and reproductive capacity of four indigenous entomopathogenic nematode (EPN) strains, S-PQ16 (Steinernema sp. PQ16), S-TX1 (Steinernema sangi TX1), S-DL13 (S. siamkayai DL13) and H-NT3 (Heterorhabditis indica NT3), against the last instar larvae of Asian corn borer (ACB), was evaluated. With moderately low concentrations of infective juveniles (IJ) from 10 to 100 IJ insect−1, the ACB larvae were found to be susceptible to all four EPN nematode strains after exposure for 24, 48, 72, 96 and 120 h. The 100% mortality of ACB larvae by these indigenous EPN strains occurred after 96-120 h of exposure. The low LC50 values (between 21.7 and 27.9 IJ) indicated the high virulence of these four EPN strains to ACB larvae. The highest yield of IJ produced inside the insect cadavers for S-PQ16, S-TX1, S-DL13 and H-NT3 was 215.0 ± 6.5 × 103, 83.1 ± 4.9 × 103, 67.8 ± 7.2 × 103 and 225.2 ± 6.4 × 103 IJ, respectively, with low inoculated concentrations of between 30-50 IJ. The results, showing a high degree of virulence and high reproduction capacity, suggest that these indigenous EPN strains have great potential for biological control of the Asian corn borer.

Molecular and Morphological Identification of Trichogramma (Hymenoptera: Trichogrammatidae) Species From Asian Corn Borer (Lepidoptera: Crambidae) in Myanmar

2020 ◽  
Author(s):  
Yee Yee Myint ◽  
Shuxiong Bai ◽  
Tiantao Zhang ◽  
Dirk Babendreier ◽  
Kanglai He ◽  
...  
Keyword(s):  
Biological Control ◽  
Species Composition ◽  
Molecular Techniques ◽  
Morphological Identification ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Feeding Sites ◽  
Corn Borers ◽  
Asian Corn Borer ◽  
Significant Obstacle

Abstract The Asian corn borer, Ostrinia furnacalis (Guenée), is the most devastating maize pest in Myanmar. The damage caused by this pest has become a significant obstacle to farmers’ income and the national economy. Control of O. furnacalis is challenging due to its protected feeding sites and the crop’s height during later phases of the pest’s attack. Trichogramma (Hymenoptera: Trichogrammatidae) parasitoids have been used successfully against corn borers in other countries, but knowledge on the species composition in Myanmar is limited. As a first step for potential biological control of Asian corn borer in Myanmar, Trichogramma were collected from major maize growing regions. Identification was performed based on both morphological and molecular techniques. The majority of collected specimens were identified as Trichogramma ostriniae Pang and Chen, T. chilonis Ishii, and T. dendrolimi Matsumura. This is the first report on the composition of Trichogramma species, and an important step towards the establishment of biological control against O. furnacalis in Myanmar.

scholarly journals The pathogenicity and reproduction capability of five epn strains on white grubs (Alissonotum impressicolle Arrow) in the laboratory conditions

2018 ◽  
Vol 15 (2) ◽  
pp. 277-284
Author(s):  
Đỗ Tuấn Anh ◽  
Nguyễn Hữu Tiền ◽  
Nguyễn Ngọc Châu
Keyword(s):  
Biological Control ◽  
Entomopathogenic Nematode ◽  
Biological Agents ◽  
Lethal Concentration ◽  
Soil Environment ◽  
White Grubs ◽  
Heterorhabditis Indica ◽  
Laboratory Conditions ◽  
High Virulence ◽  
Mortality Percentage

Five entomopathogenic nematode strains, included 3 strains of the genus Steinernema, e.g. S-PQ16, SS-CP12 and S-TX1, and two strains of genus Heterorhabditis indica, e.g. H-CB3452 and H-KT3987 were evaluated on virulence and reproduction capacity on white grubs of black scarab (Alissonotum impressicolle Arrow) a serious pest damaging in the soils of sugarcane and many economic crops in Western Highland, particular Lam Dong province. The experiments were evaluated the pathogenicity virulence of EPN strains through establishing index as lethal concentration of 50 mortality percentage of host insects (LC50). While the reproduction capacity of EPN strains were established yield of infective juveniles (IJs) that produced inside insect cadavers. The bioassays on virulence of S-PQ16, S-CP12, S-TX1, H-CB3452 and H-KT3987 were revealed the mortality of white grubs as 93.3%, 86.7%; 93.3%; 86.7% and 73.3%, respectively, at the highest concentration of 5,000 IJs/insect. The 50% mortality of five indigenous strains was high levels with LC50 values ranged between 1,362 and 2,725 IJs. These values are also similar with our results previously on white grubs with some other EPN indigenous strains. It is also suitable with some evaluation bioassays of EPN on white grubs reported from China and Australia. The IJs yields from the insect cadavers were obtained up to 31×103 IJs with the strain S- CP12; 59.7×103 IJs with the S-PQ16 strain and 73.5×103 IJs with the strain S-TX1. Particularly, the highest yields were obtained from two strains of Heterorhabditis indica, viz. 125.1×103 IJs with H-KT3987 strain and 112.6×103 IJs with H-CB3452 strains. Respect to high virulence and also high reproduction capacity all these EPN strains should be satisfied the biological agents that can be used for biological control of white grubs of pests are living in the soil environment.

scholarly journals Trichogramma ostriniae Is More Effective Than Trichogramma dendrolimi As a Biocontrol Agent of the Asian Corn Borer, Ostrinia furnacalis

Insects ◽  
2022 ◽  
Vol 13 (1) ◽  
pp. 70
Author(s):  
Yu Wang ◽  
Yang-Yang Hou ◽  
Giovanni Benelli ◽  
Nicolas Desneux ◽  
Asad Ali ◽  
...  
Keyword(s):  
Biocontrol Agent ◽  
Developmental Time ◽  
Economic Losses ◽  
Ostrinia Furnacalis ◽  
Female Progeny ◽  
Corn Borer ◽  
High Preference ◽  
Asian Corn Borer ◽  
Biocontrol Potential ◽  
Trichogramma Dendrolimi

The Asian corn borer (ACB), Ostrinia furnicalis, is a serious corn pest in south-east Asia, causing huge economic losses every year. Trichogramma dendrolimi and Trichogramma ostriniae, two egg parasitoids, have previously been identified as key biological control agents. To determine the age impact of ACB eggs on their effective biocontrol potential, herein we compared the biological parameters (i.e., number of parasitized eggs, emergence, developmental time, and sex ratio) of both parasitoids on ACB eggs of various ages (i.e., 0–4, 4–8, 8–12, 12–16, 16–24, 24–36, and 36–48 h old), respectively. Our results showed that the age of ACB eggs had a significant impact on the parasitization activity of T. dendrolimi in both choice and no-choice conditions. Trichogramma dendrolimi preferred to parasitize 0–8-h-old ACB eggs, and its parasitization dramatically declined on ACB eggs older than 8 h under choice and no-choice conditions. On the other hand, T. ostriniae showed high preference to parasitize all tested ACB egg ages. The age of ACB eggs had no significant impact on the parasitization of T. ostriniae under choice and no-choice conditions. Furthermore, the female progeny of T. dendrolimi decreased as the age of ACB increased, while no differences were found in female progeny of T. ostriniae. Trichogramma ostriniae also developed faster on each ACB egg age group in comparison with T. dendrolimi. Overall, the age of ACB eggs had a significant impact on T. dendrolimi performance, leading us to conclude that T. ostriniae is more effective than T. dendrolimi as a biocontrol agent of the ACB.

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