Cryptaphelenchus abietis n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from Cryphalus piceae (Ratzeburg) (Coleoptera: Scolytinae) emerged from Abies veitchii Lindl. (Pinaceae) from Nagano, Japan

Nematology ◽  
2021 ◽  
pp. 1-20
Author(s):  
Natsumi Kanzaki ◽  
Taisuke Ekino ◽  
Yousuke Degawa
Keyword(s):  
New Species ◽  
Ventral View ◽  
Posterior Part ◽  
Morphometric Characters ◽  
Abies Veitchii ◽  
Molecular Sequence ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Tail Tip ◽  
Male Genital

Summary An undescribed Cryptaphelenchus species was isolated from a bark beetle, Cryphalus piceae that had emerged from a dead log of the Veitch’s fir, Abies veitchii, collected from Sugadaira Montane Research Center, University of Tsukuba, Nagano, Japan. The new species is characterised by its female post-vulval uterine sac (PUS) of less than one vulval body diam. in length and female posterior body end (tail) conical with elongate posterior part and variably shaped tip, seven male genital papillae, which are composed/arranged as a precloacal P1, P2 pair slightly anterior to cloacal opening, and two small pairs of glandpapillae near tail tip and very narrow bursal flap-like cuticular extension surrounding the male tail. In addition to these characters, the male apophysis appears wing-like in ventral view. In possessing an elongate posterior part, Cryptaphelenchus abietis n. sp. shares the female tail shape with C. borlossi, C. leptocaudus and C. sutoricus, and shares a short PUS with C. iranicus, C. varicaudatus, C. baujardi and C. paravaricaudatus. However, the new species can be distinguished from these species by other typological and morphometric characters and its molecular phylogenetic status. Phylogenetically, the new species is closest to Bursaphelenchus minutus, but can be clearly distinguished by morphological and molecular sequence characters.

Description of two Pseudaphelenchus species (Tylenchomorpha: Aphelenchoididae) associated with Asian termites and proposal of Tylaphelenchinae n. subfam.

Nematology ◽  
2014 ◽  
Vol 16 (8) ◽  
pp. 963-978 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Hou-Feng Li ◽  
Yen-Chiu Lan ◽  
Robin M. Giblin-Davis
Keyword(s):  
New Species ◽  
Small Body ◽  
Morphological Characters ◽  
Small Subunit ◽  
Coptotermes Formosanus ◽  
Phylogenetic Status ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Tail Tip

Two new Pseudaphelenchus species were discovered from subterranean and arboreal termites from the East Asian subtropics. Pseudaphelenchus sui n. sp. isolated from Coptotermes formosanus was collected from Miyako Isl., Okinawa, Japan, and P. scheffrahni n. sp. was isolated from Nasutitermes takasagoensis collected from the Kenting National Park in Taiwan. The two new species and P. vindai, previously described from Panamanian termites, are close to each other, and are not easily distinguished morphologically, i.e., these three are almost ‘cryptic species’. However, they can be distinguished based on minor morphological differences, including shape of male bursa (clearest in P. vindai, vague in P. sui n. sp. and intermediate in P. scheffrahni n. sp.), male tail tip (P. sui n. sp. often have a small mucron but the others do not), and in the character of the female tail (the two new species have stronger ventral curvature than P. vindai, and further, P. sui n. sp. has clear annulation at distal part, while the other species do not). Molecular phylogenetic analysis based upon near-full-length sequences of the small subunit of the ribosomal RNA gene suggested that Pseudaphelenchus and Tylaphelenchus form a strongly-supported clade at the base of the family Aphelenchoididae, and that Tylaphelenchus is included in Pseudaphelenchus as an inner clade. Therefore, based on their phylogenetic status and common morphological characters, e.g., small body and spherical median bulb, a subfamily, Tylaphelenchinae n. subfam. is proposed to embrace these two genera. In addition, Ruehmaphelenchus ipidicola n. comb. (= Tylaphelenchus ipidicola) is proposed.

Description of Ditylenchus acantholimonis n. sp. (Rhabditida: Anguinidae) from Iran, a morphological and molecular phylogenetic study

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Samira Aliverdi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip

Summary Ditylenchus acantholimonis n. sp. is described based on morphological, morphometric and molecular characters. It was isolated from the rhizosphere soil of Acantholimon sp. in Golestan province, Iran, and is mainly characterised by having four lines in the lateral field, a pyriform to bottle-shaped offset pharyngeal bulb, post-vulval uterine sac 36.6-56.1% of the vulva to anus distance long, and a subcylindrical to conical tail with widely rounded tip. It is further characterised by short to medium-sized females, 480-617 μm long, with a fine stylet having small rounded knobs, V = 80.8-83.6, c = 11.0-13.8, c′ = 3.3-4.6, and males with 16.0-17.0 μm long spicules. The new species was morphologically compared with six species having four lines in their lateral field, rounded tail tip and comparable morphometric data namely: D. dipsacoideus, D. emus, D. exilis, D. paraparvus, D. sturhani, and D. solani. It was also compared with two species, D. ferepolitor and D. angustus, forming a maximally supported clade in the 18S tree. The phylogenetic analyses using the maximal number of Anguinidae and several Sphaerularioidea genera based upon partial 18S and 28S rDNA D2-D3 sequences revealed that Ditylenchus is polyphyletic. In the 18S tree, the new species formed a clade with D. ferepolitor (KJ636374) and D. angustus (AJ966483); in the 28S tree it formed a poorly supported clade with D. phyllobios (KT192618) and Ditylenchus sp. (MG865719).

Bursaphelenchus rufipennis n. sp. (Nematoda: Parasitaphelenchinae) and redescription of Ektaphelenchus obtusus (Nematoda: Ektaphelenchinae), associates from nematangia on the hind wings of Dendroctonus rufipennis (Coleoptera: Scolytidae)

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 925-955 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Robin M. Giblin-Davis ◽  
Yasmin J. Cardoza ◽  
Weimin Ye ◽  
Kenneth F. Raffa ◽  
...  
Keyword(s):  
New Species ◽  
Morphological Characters ◽  
Morphological Examination ◽  
Dendroctonus Rufipennis ◽  
Lateral Field ◽  
The Past ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Voucher Specimens ◽  
Tail Tip

Abstract Two species of aphelench, Bursaphelenchus rufipennis n. sp. and Ektaphelenchus obtusus, were isolated from the 'nematangia', cocoon-like structures found at the base of the hind wings of Dendroctonus rufipennis. The nematangia contained adult females of E. obtusus and the dauer juveniles of B. rufipennis n. sp. Only B. rufipennis n. sp. could be cultured on Monilinia fructicola on LGPDA (lactic acid-treated, glycerol-supplemented, potato dextrose agar). The new species of Bursaphelenchus is described and figured and some additional morphological characters are ascribed to E. obtusus, E. josephi, E. sandiaensis, E. smaelus (= E. prolobos) and E. terebranus after examination of type and/or voucher specimens. Bursaphelenchus rufipennis n. sp. has an adult body length of ca 500-1000 μm, medium a ratios (ca 25-38 for females and ca 30-40 for males), b ratios of ca 8-13 (female) and 7-11 (male), c ratios of ca 15-22 (female and male), c′ ratios of ca 3-4 (female) and ca 2-3 (male), and is characterised by three incisures in the lateral field, mitten-shaped spicules and a conical female tail that curves ventrally and possesses a variable tail tip. The new species is morphologically closest to B. corneolus, B. curvicaudatus, B. gerberae, B. paracorneolus and B. talonus. Morphological examination of type and/or voucher specimens of five Ektaphelenchus species revealed coarse transverse body annulation and three pairs of male caudal papillae (except for the two species where males are not described). Clear typological differences were observed among these five Ektaphelenchus species in the structure of the lip region, presence/absence of stylet knobs and male spicule morphology. Although these characters have not been consistently documented in the past, they may be diagnostic for species in the genus. Molecular phylogenetic analysis based on SSU and D2/D3 LSU sequences revealed that B. rufipennis n. sp. was closest to B. paracorneolus and that E. obtusus was closest to species of Ektaphelenchoides and a Cryptaphelenchus sp.

scholarly journals Taxonomical considerations and molecular phylogeny of the closely related genera Bitylenchus, Sauertylenchus and Tylenchorhynchus (Nematoda: Telotylenchinae), with one new and four known species from Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
M. Hosseinvand ◽  
A. Eskandari ◽  
S. Ganjkhanloo ◽  
R. Ghaderi ◽  
P. Castillo ◽  
...  
Keyword(s):  
New Species ◽  
Anterior Part ◽  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Molecular Data ◽  
28S Rrna ◽  
Natural Habitats ◽  
Morphometric Characters ◽  
Molecular Approaches ◽  
Tail Tip

Abstract During several nematological surveys in cultivated and natural habitats in Khuzestan and Zanjan provinces of Iran, a new species, Bitylenchus parvulus n. sp., two new records for Iran – namely, Tylenchorhynchus agri and Tylenchorhynchus graciliformis – and a population of Bitylenchus parvus and one of Sauertylenchus maximus were recovered and characterized based upon morphological and molecular approaches. The new species is characterized by lip region with five to seven annuli, stylet 17.7 (17.0–18.5) μm long, sub-cylindrical tail narrowing abruptly near terminus giving a bluntly digitate shape to the tail tip, cuticle near anterior part of vulva wrinkled and post-rectal sac occupies whole of tail cavity. The phylogenetic analyses were carried out using molecular data from D2–D3 expansion segments of large ribosomal subunit (28S rRNA) for all studied species and the partial small ribosomal subunit (18S rRNA) for the new species. The representatives of Bitylenchus and Sauertylenchus formed distinct clades from Tylenchorhynchus members, supporting the hypothesis in which Bitylenchus and Sauertylenchus could be considered as valid genera, but rejecting the ‘large-genus’ concept for Tylenchorhynchus. Also, Sauertylenchus ibericus was proposed as a junior synonym of S. maximus based on the results from morphological and phylogenetic analysis. Furthermore, an identification key for all known species included in the three genera Bitylenchus, Tylenchorhynchus and Sauertylenchus is presented herein. The number of transverse annuli on the lip region and presence/absence of post-rectal sac were considered as the main diagnostic characters for classifying the species into seven groups, and other morphological and morphometric characters were subsequently used for distinguishing species in each group.

Acrostichus puri n. sp. (Nematoda: Diplogastridae), a phoretic associate of Augochlora pura mosieri Cockerell (Hymenoptera: Halictidae)

Nematology ◽  
2010 ◽  
Vol 12 (1) ◽  
pp. 49-64 ◽  
Author(s):  
Yongsan Zeng ◽  
W. Kelley Thomas ◽  
Natsumi Kanzaki ◽  
Barbara Center ◽  
Robin Giblin-Davis ◽  
...  
Keyword(s):  
New Species ◽  
Anterior Part ◽  
Posterior Part ◽  
The Body ◽  
Sweat Bee ◽  
Molecular Sequence ◽  
Female Tail ◽  
Triangular Flap ◽  
Mating Tests

AbstractAcrostichus puri n. sp., isolated from the body of a sweat bee, Augochlora pura mosieri, is described and figured. The new species is morphologically, molecularly and biologically very similar to A. halicti, i.e., these two species share a very complicated gubernaculum structure, distally hooked spicules with a triangular flap-like rostrum, stomatal polymorphism and synchronised associations with halcitid bees. The new species can be distinguished from A. halicti by its spicule morphology, distinctive squared manubrium and sharply bent (= ca 90° or L-shaped) distal tip vs an indistinctive rounded manubrium and a strongly recurved (= ca 180° or U-shaped) distal tip; gubernaculum with narrower, longer, anterior part and more complex posterior part than A. halicti, and tail of both sexes, with a long and filiform spike on the male or female tail vs a short spike (male) or conical tail (female). These two species also differ from each other by significant molecular sequence differences in SSU (2.0%), D2/D3 LSU (4.5%) and mtCOI (9.2%), and are reproductively incompatible in mating tests.

scholarly journals ThegenusAnasa from Ecuador with description of three new species and key to the Ecuadorian species (Hemiptera: Heteroptera: Coreidae)

2017 ◽  
Vol 57 (1) ◽  
pp. 61-71
Author(s):  
Harry Brailovsky
Keyword(s):  
New Species ◽  
Morphological Traits ◽  
Ventral View ◽  
Three New Species ◽  
Male Genital

Abstract Three new species of Anasa Amyot & Serville, 1843 (Coreidae: Coreinae: Coreini), A. cotopaxiana sp. nov., A. crinita sp. nov., and A. humerata sp. nov., from Ecuador are described and illustrated in dorsal and some in ventral view. The new species are distinguished by a combination of colouration patterns and certain morphological traits, such as the shape of the pronotum and the posteroventral edge of the male genital capsule, as well as the armature of the antenniferous tubercles and femora. Drawings of the male genital capsules and a revised key for the sixteen known species of Anasa from Ecuador are also provided.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Acrostichus megaloptae n. sp. (Nematoda: Diplogastridae), a phoretic associate of Megalopta spp. (Hymenoptera: Halictidae) in Central America

Nematology ◽  
2010 ◽  
Vol 12 (3) ◽  
pp. 453-468 ◽  
Author(s):  
Kelley Thomas ◽  
Robin Giblin-Davis ◽  
William Wcislo ◽  
Natsumi Kanzaki ◽  
Yongsan Zeng ◽  
...  
Keyword(s):  
New Species ◽  
Ventral View ◽  
Lateral View ◽  
Barro Colorado Island ◽  
La Selva ◽  
Megalopta Genalis ◽  
Rotting Wood ◽  
Stomatal Morphology ◽  
Molecular Phylogenetic ◽  
Halictus Ligatus

AbstractAdults of the dusk-flying and rotting wood-nesting bees Megalopta genalis and/or M. ecuadoria were collected at Barro Colorado Island, The Smithsonian Tropical Research Institute (BCI, STRI), Panama and La Selva, Costa Rica, and examined for nematodes. Nematodes were recovered as dauer juveniles from the Dufour's gland of female bees and aedeagus of male bees. Adult nematodes isolated from M. genalis (BCI and La Selva) and M. ecuadoria (BCI) were successfully cultured and kept in the laboratory. All three populations were used for morphological observations and molecular analyses and were identified as a new species that is described herein as Acrostichus megaloptae n. sp. It is characterised by its stomatal morphology, possession of six triangular cuticle flaps covering stomatal opening, duplicated cheilo- and gymnostomatal walls, large dorsal tooth and sclerotised ventral stegostomatal ridges, female with vulval flap, male spicule and gubernaculum morphology, i.e., relatively straight spicule with oval-shaped manubrium, rounded flap-like rostrum and separated and strongly ventrally curved spicule with bifurcate tip, gubernaculum L-shaped in lateral view and anchor-shaped in ventral view, and filiform tail of both sexes. Sequences from the three A. megaloptae n. sp. geographical/host isolates were not significantly different and molecular phylogenetic analysis and biological and morphological comparisons place the new species close to A. halicti from Halictus ligatus and A. puri from Augochlora pura from North America, although the new species is distinguished from A. halicti and A. puri based upon its stomatal morphology of males and females, male spicule and gubernaculum morphology and female vulval structure.

Sheraphelenchus sucus n. sp. (Tylenchina: Aphelenchoididae) isolated from sap flow of Quercus serrata in Japan

Nematology ◽  
2013 ◽  
Vol 15 (8) ◽  
pp. 975-990 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Ryusei Tanaka
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Sap Flow ◽  
Additional Data ◽  
Quercus Serrata ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Molecular Barcode ◽  
Rna Genes

A Sheraphelenchus species was isolated from a sample of sap exuding from a scar on the bark of Quercus serrata. Besides its generic characteristics, i.e., posteriorly located vulva in the female, male spicule with conspicuous dorsal limb and male tail with spike-like projection, the new species is characterised by a short stylet with a small basal swelling. The near-full-length of 18S and D2/D3 expansion segments of ribosomal RNA genes (near-full SSU and D2/D3LSU) were determined as its molecular barcode sequences and the phylogenetic status of the species (= genus Sheraphelenchus) was estimated using the near-full SSU. The molecular phylogenetic analysis revealed that the new species is included in the genus Bursaphelenchus, and is close to B. anatolius and B. kevini. Although Sheraphelenchus is phylogenetically inferred to occur within Bursaphelenchus, the new species is described herein as S. sucus n. sp., thereby retaining the genus Sheraphelenchus until additional data about morphological similarities between these two taxa are obtained.

Description of Bursaphelenchus parvispicularis n. sp. (Nematoda: Parasitaphelenchidae) isolated from a dead oak tree, Quercus mongolica var. grosseserrata

Nematology ◽  
2005 ◽  
Vol 7 (5) ◽  
pp. 751-759 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
New Species ◽  
Botrytis Cinerea ◽  
Dorsal Surface ◽  
Lateral Field ◽  
Quercus Mongolica ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Oak Tree ◽  
Tail Tip ◽  
Group Species

AbstractBursaphelenchus parvispicularis n. sp. is described and figured. The new species was isolated from the bark of a dead oak tree, Quercus mongolica var. grosseserrata, and reared on Botrytis cinerea. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species has a body length of 894 (711-1012) μm in the female and 764 (600-870) μm in the male, a ratio of 33.4 (29.7-37.1) in the female and 35.6 (31.4-45.3) in the male, and c and c′ ratios of 16.7 (14.8-19.0) and 4.4 (3.9-5.1), respectively, in the female and 24.3 (20.0-28.4) and 2.5 (2.2-2.8), respectively, in the male. The stylet is 14-16 μm long in the female and 13-15 μm in the male; there are three lines in the lateral field; the spicules are small (13 (13-15) μm long) and broad; there are seven (2 + 1 + 2 + 2) male caudal papillae; the 'bursa' varies from roundish trapezoid to rectangular with a concave terminus, and the female tail is tapered with clearly annulated dorsal surface near the tail tip and a rounded terminus. Based upon morphology the new species belongs to the B. hofmanni-group sensu Braasch. However, the new species is distinguished from the other B. hofmanni-group species by the size and form of the spicule, female tail shape, and morphometric values. Based on the molecular phylogenetic analysis of the DNA base sequences of ribosomal DNA and mtCOI gene, the new species was close to two B. hofmanni-group species (B. paracorneolus and B. hofmanni) and to B. hylobianum. This result is in accordance with their morphological similarity.

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