Variations in Acorn Traits in Two Oak Species: Quercus mongolica Fisch. ex Ledeb. and Quercus variabilis Blume

Quercus mongolica Fisch. ex Ledeb. and Q. variabilis Blume are two main oak species in China, producing large amounts of acorns every year. However, the trait variations in acorns, as a promising energy crop material, are not fully understood, hence we compared the traits of acorns from the different populations with the altered geographic distribution in this study. Thirteen acorn traits, including phenotype, proximate compositions and functional compounds, were analyzed in both Quercus L. species collected from 44 populations across China. The results showed that, except large differences found among accessions in acorn sizes, the starch varied from 140.96–297.09 mg/g in Q. mongolica and 130.99–306.28 mg/g in Q. variabilis, indicating its substantial differences among populations. The total polyphenols, total flavonoids and soluble tannins varied from 41.76–158.92, 23.43–91.94, and 15.11–17.81 mg/g, respectively, in Q. mongolica, 89.36–188.37, 50.59–116.07, 15.24–17.33 mg/g, respectively, in Q. variabilis, demonstrating their large variations in the levels of polyphenols among populations. Moreover, the acorns of Q. mongolica in North China and Q. variabilis in Southwest China had higher levels of starch and polyphenols. As the geographical location approached in the distribution of two Quercus species, the difference in acorn sizes gradually increased, while that in polyphenols were opposite. Principal component analysis and cluster analysis further revealed that the acorn sizes became larger and polyphenols became less with the increasing latitudes in both species. In North China, the acorns of Q. mongolica had small sizes and high polyphenols, which was contrary to those in Q. variabilis. These findings indicated that acorn traits were closely associated with the geographical distribution. Thus, our results will provide references for the selection breeding of acorn with the high starch, high or low polyphenols in the different regions.

Composition of Organic Carbon-Based Compounds in the Stem Wood of Quercus mongolica Seedlings Grown Under Elevated CO2 and/or O3 Concentrations

In this study, we examined the composition of organic constituents of stem woody tissue together with tree growth in Quercus mongolica var. grosseserrata Blume seedlings raised under controlled CO2 and/or O3 concentrations in a Free-Air Concentration Enrichment system. After exposure to ambient air (control), elevated CO2 concentration (550 μmol mol–1 CO2), elevated O3 concentration (double that of the control), and a combination of elevated CO2 and O3 concentrations during a growing season, we measured the diameter and length of stem, and biomass of sampled seedlings and quantified the lignin, extractive, and holocellulose contents of the woody tissue of current-year stems. We confirmed that the growth of seedlings was enhanced under an elevated CO2 concentration condition. In line with this, the extractive content was lower in woody tissue formed under an elevated CO2 concentration than that formed under ambient air, whereas holocellulose content showed an inverse pattern. Elevated O3 concentration itself did not change the organic constituents of the woody tissue, but it reduced the influence of an elevated CO2 concentration. We thus assume that Q. mongolica formed woody tissue with a low extractive content under the high CO2 concentration condition, although this response was possibly mitigated by an elevated O3 concentration. Extractives contains antimicrobial components such as tannins, flavonoids, quinones, and terpenoids. The decrease in extractives within the widely distributed Q. mongolica in East Asia may have a non-negligible impact on C cycling in the future earth with high atmospheric CO2 concentration.

Ectomycorrhizal Inoculation Enhances the Salt Tolerance of Quercus mongolica Seedlings

Salt stress harms the growth and development of plants, and the degree of soil salinization in North China is becoming increasingly severe. Ectomycorrhiza (ECM) is a symbiotic system formed by fungi and plants that can improve the growth and salt tolerance of plants. No studies to date have examined the salt tolerance of Quercus mongolica, a typical ectomycorrhizal tree species of temperate forests in the northern hemisphere. Here, we inoculated Q. mongolica with two ectomycorrhizal fungi (Gomphidius viscidus; Suillus luteus) under NaCl stress to characterize the effects of ECM. The results showed that the symbiotic relationship of Q. mongolica with G. viscidus was more stable than that with S. luteus. The cross-sectional area of roots increased after inoculation with the two types of ectomycorrhizal fungi. Compared with the control group, plant height, soluble sugar content, and soluble protein content of leaves were 1.62, 2.41, and 2.04 times higher in the G. viscidus group, respectively. Chlorophyll (Chl) content, stomatal conductance (Gs), and intracellular CO2 concentration (Ci) were significantly higher in Q. mongolica inoculated with ectomycorrhizal fungi than in the control, but differences in the net photosynthetic rate (Pn), transpiration rate (Tr), and photosystem II maximum photochemical efficiency (Fv/Fm) were lower. The relative conductivity of Q. mongolica inoculated with the two ectomycorrhizal fungi was consistently lower than that of non-mycorrhizal seedlings, with the effect of G. viscidus more pronounced than that of S. luteus. The malondialdehyde (MDA) content showed a similar pattern. Peroxidase (POD) and catylase (CAT) levels in mycorrhizal seedlings were generally higher than those of non-mycorrhizal seedlings under normal conditions, and were significantly higher than those of non-mycorrhizal seedlings on the 36th and 48th day after salt treatment, respectively. Overall, the results indicated that the salt tolerance of Q. mongolica seedlings was improved by ectomycorrhizal inoculation.

Species-Specific Nitrogen Resorption Efficiency in Quercus mongolica and Acer mono in Response to Elevated CO2 and Soil N Deficiency

To test the effects of elevated CO2 and soil N deficiency on N resorption efficiency (NRE) from senescing leaves in two non-N2-fixing deciduous broadleaved tree species, Japanese oak (Quercus mongolica var. grosseserrata Blume) and Painted maple (Acer mono Maxim. var. glabrum (Lév. Et Van’t.) Hara), potted seedlings were grown in a natural daylight phytotron with either ambient or elevated CO2 conditions (36 Pa and 72 Pa CO2) and with two levels of N (52.5 and 5.25 mg N pot−1 week−1 for high N and low N, respectively). We examined the N content (Nmass) of mature and senescent leaves, as well as photosynthesis and the growth of plants, and calculated both the mass-based NRE (NREmass) and leaf area-based NRE (NREarea). In both species, the Nmass of mature leaves decreased with high CO2 and low N, whereas the leaf mass per area (LMA) increased under elevated CO2, regardless of N treatments. In Q. mongolica, both the maximum rate of carboxylation (Vcmax) and the maximum electron transport rate (Jmax) were reduced by elevated CO2 and low N, but Vcmax exhibited an interactive effect of N and CO2 treatments. However, in A. mono, both the Vcmax and Jmax decreased under elevated CO2, regardless of N treatments. The partitioning of N for the photosynthetic function within leaves was also significantly decreased by elevated CO2 in both species and increased under low N in A. mono. The Nmass of senesced leaves decreased under low N in both species and exhibited an increase (Q. mongolica) or no effect (A. mono) by elevated CO2. The NREarea of Q. mongolica was affected by CO2 and N treatments, with a decrease under elevated CO2 compared to ambient CO2 and under low N compared to high N. The NREarea of A. mono was also affected by CO2 and N treatments and decreased under elevated CO2; however, unlike in the case of Q. mongolica, it increased under low N. We speculate that these interspecific differences in the responses of leaf N allocation, indicated by the photosynthetic (Vcmax and Jmax) and morphological (LMA) responses to elevated CO2, may have affected the NRE during defoliation under high CO2 and soil N-deficient conditions.