Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Molecular and morphological characterisation of Laimaphelenchus spiflatus n. sp. (Nematoda: Aphelenchoididae) from China

Nematology ◽  
2020 ◽  
Vol 22 (8) ◽  
pp. 843-853
Author(s):  
Jianfeng Gu ◽  
Munawar Maria ◽  
Yiwu Fang ◽  
Xianfeng Chen ◽  
Lele Liu
Keyword(s):  
New Species ◽  
Body Size ◽  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
The Other ◽  
Morphometric Data ◽  
Lateral Field ◽  
Chinese Pine ◽  
Lateral Lines ◽  
Genomic Regions

Summary Laimaphelenchus spiflatus n. sp. isolated from declining Chinese pine, Pinus tabuliformis, is described and illustrated. The new species can be characterised by its relatively long body size of 1150 ± 108 (976-1437) μm for females and 1092 ± 78.6 (905-1235) μm for males, lateral field with four lines, females with a long vulval flap, and tail conoid, slightly ventrally curved and ending in a stalk having 8-12 projections when observed with SEM. Males are common, with 27.3 (23.4-28.8) μm long spicules having blunt (not well-developed) condylus and rostrum and truncate simple distal tip, and four caudal papillae. By having a vulval flap, four lateral lines and tail end with a stalk (without four tubercles), the new species comes close to L. preissii, L. simlaensis, and L. unituberculus, but can be differentiated from them by morphological characters and morphometric data. In phylogenetic analyses using near full length 18S and D2-D3 expansion segments of 28S rDNA, Laimaphelenchus spiflatus n. sp. appeared as an independent lineage separated from the other Laimaphelenchus spp. that are currently sequenced for their aforementioned genomic regions.

Description of Bursaphelenchus arthuroides sp. n. (Nematoda: Aphelenchoididae), a second parthenogenetic species of Bursaphelenchus Fuchs, 1937

Nematology ◽  
2012 ◽  
Vol 14 (1) ◽  
pp. 51-63 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Jingwu Zheng
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Dorsal Part ◽  
Species Status ◽  
Lateral Field ◽  
Middle Position ◽  
Parthenogenetic Species ◽  
Female Tail ◽  
Molecular Phylogenetic

Bursaphelenchus arthuroides sp. n. is described and figured from pine packaging wood originating in Brazil, and inspected in Ningbo harbour, P. R. China. The new species clearly belongs to the fungivorus group. It is characterised by four lines in the lateral field; spicules medium sized (15-17 μm), dorsal part distinctly sclerotised, with a high rounded condylus and a blunted pointed rostrum in the middle position, capitulum with a shallow depression, the posterior third of the dorsal limb ventrally bent, distal end broadly rounded without cucullus; the shape of the female tail conical and slim, posterior third distinctly ventrally bent with a finely pointed terminus; vulval lips slightly protruding but not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. Species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on partial LSU sequences, ITS1/2 and partial LSU sequences, which revealed that B. arthuroides sp. n. is closest to B. arthuri. Propagation tests also confirmed that the new species is parthenogenetic.

Description of Bursaphelenchus paraparvispicularis n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Hongkong, China

Nematology ◽  
2010 ◽  
Vol 12 (4) ◽  
pp. 557-566 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang ◽  
Weijun Duan ◽  
Helen Braasch ◽  
Wolfgang Burgermeister ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Phylogenetic Analysis ◽  
Species Status ◽  
Separate Species ◽  
Female Ratio ◽  
Lateral Field ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Abstract Bursaphelenchus paraparvispicularis n. sp. is described and figured from pine packaging wood originating in Hongkong, China, and inspected in Ningbo harbour, China. The new species clearly belongs to the hofmanni group. It is characterised by a relatively stout body (a = 26.7 and 26.5 for males and females, respectively), three lines in the lateral field, seven caudal papillae, spicules relatively small (12.6-15.3 μm), mitten-shaped, with lamina dorsal line smoothly arcuate but calomus relatively straight, condylus squared or round, well developed, rostrum well developed with round terminus, cucullus absent, the shape of the female tail, which is short and ventrally bent with a bluntly pointed terminus, and vulval lips not forming a vulval flap. The new species is morphologically closest to B. parvispicularis and can be distinguished by smaller and stouter body, lower female ratio c′ (average 2.8 vs 4.4) and longer spicule condylus. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 and partial LSU sequences, which revealed that B. paraparvispicularis n. sp. is closest to B. parvispicularis.

Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

Description and phylogeny of Bursaphelenchus luxuriosae n. sp. (Nematoda: Aphelenchoididae) isolated from Acalolepta luxuriosa (Coleoptera: Cerambycidae)

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 565-572 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Kazuyoshi Futai
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Botrytis Cinerea ◽  
Bursaphelenchus Xylophilus ◽  
Lateral Field ◽  
Female Tail ◽  
Phylogenetic Status ◽  
Rflp Profile ◽  
Molecular Phylogenetic ◽  
Males And Females

AbstractBursaphelenchus luxuriosae n. sp. is described and figured. Specimens were collected from a 2-week-old culture on Botrytis cinerea. The new species is characterised by a body length of 897 (710-1159) μm in the female and 745 (621-887) μm in the male, relatively robust body (a = 33-39 in the female and 27-30 in the male), stylet ca 14 (11-16) μm long, four lines in the lateral field, the large (27-30 μm) arcuate spicule with a terminal cucullus, seven (2 + 1 + 2 + 2) male caudal papillae, the long, well developed vulval flap and the shape of the female tail which is long, tapered, and ventrally bent when killed by heat with an irregular or roughened dorsal contour near the tip and an irregular terminus. The new species is considered to belong to the Bursaphelenchus xylophilus group of the genus Bursaphelenchus and is most closely related to B. conicaudatus and B. fraudulentus in spicule shape, vulval flap and 'a' values of males and females. It is easily distinguished from these two species by the morphology of female tail. The RFLP profile confirms the distinctness of the new species within the B. xylophilus group. The phylogenetic status of B. luxuriosae n. sp. within the B. xylophilus group is indicated by molecular phylogenetic analysis. Bursaphelenchus luxuriosae n. sp. is assumed to be close to B. conicaudatus and to have diverged from the ancestor of the B. xylophilus group early in the speciation of the group.

Bursaphelenchus antoniae sp. n. (Nematoda: Parasitaphelenchidae) associated with Hylobius sp. from Pinus pinaster in Portugal

Nematology ◽  
2006 ◽  
Vol 8 (5) ◽  
pp. 659-669 ◽  
Author(s):  
Ana Catarina Penas ◽  
Kai Metge ◽  
Manuel Mota ◽  
Vera Valadas
Keyword(s):  
New Species ◽  
Pinus Pinaster ◽  
Small Body ◽  
Petri Dish ◽  
The Body ◽  
Monilinia Fructicola ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
Female Tail ◽  
18S Rdna Sequence

Abstract Bursaphelenchus antoniae sp. n. is described and illustrated. Dauer juveniles were isolated from the body of the large pine weevil, Hylobius sp., collected from maritime pine (Pinus pinaster) stumps, in Portugal. Bursaphelenchus antoniae sp. n. was reared and maintained in P. pinaster wood segments and on Petri dish cultures of the fungi Botrytis cinerea and Monilinia fructicola. The new species is characterised by a relatively small body length of ca 583 μ m (females) and 578 μ m (males), a lateral field with two incisures, presence of a small vulval flap and a conoid female tail with a rounded or pointed terminus. Males have stout spicules with a disc-like cucullus and seven caudal papillae arranged as a single midventral precloacal papilla, one precloacal pair and two postcloacal pairs. In the character of the lateral field, B. antoniae sp. n. comes close to B. abietinus, B. rainulfi and B. hylobianum, whilst spicule characters place it within the piniperdae-group sensu Ryss et al. Morphologically, B. antoniae sp. n. is closest to B. hylobianum; the spicules of these two species having flattened, wing-like, alae on the distal third of the lamina. Bursaphelenchus antoniae sp. n. is distinguished from B. hylobianum on the arrangement of the caudal papillae (two vs three pairs). ITS-RFLP profiles and the failure to hybridise support the separation of the two species. Phylogenetic analysis of the new species, based on the 18S rDNA sequence, supports the inclusion of this new species in the B. hylobianum-group sensu Braasch. Sequence analysis of the 28S rDNA D2/D3 domain did not place the new species in a definite group.

Description of Ruehmaphelenchus digitulus sp. n. (Nematoda: Aphelenchoididae) found in packaging wood from Taiwan

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 489-498 ◽  
Author(s):  
Jianfeng Gu ◽  
Jiangling Wang
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Rflp Pattern ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Shape And Size

Ruehmaphelenchus digitulus sp. n., isolated from coniferous packaging wood from Taiwan, is described and figured. The new species is characterised by a relatively stout body with a = 28.0 (23.7-31.3) and 27.0 (22.9-32.7) for male and female, respectively, three lines in the lateral field, spicules relatively small (chord = 17.0-19.2 μm; arc = 13.8-16.6 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 80% of body length, vulva flap absent, vulval lips slightly protruding, post-uterine branch extending slightly over half of vulva to anus distance, and tail conoid, ca 2-3 anal body diam. long, with terminal process 7-10 μm long. The new species is the fourth known for the genus. It can be distinguished from R. martinii, R. asiaticus and R. thailandae mainly by the different shape and size of the spicules and the different shape and length of the female tail terminus. The separate species status is also supported by ITS-RFLP pattern and molecular phylogenetic analysis based on partial SSU ITS1/2 and partial LSU sequences.

Description of Bursaphelenchus yuyaoensis n. sp. (Nematoda: Aphelenchoididae) isolated from Pinus massoniana in China

Nematology ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. 411-418 ◽  
Author(s):  
Jianfeng Gu ◽  
Jie He ◽  
Jiangling Wang ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Pinus Massoniana ◽  
Species Status ◽  
Separate Species ◽  
Lateral Field ◽  
Male Tail ◽  
Female Tail ◽  
Molecular Phylogenetic ◽  
Males And Females

Bursaphelenchus yuyaoensisn. sp. is described and figured from dyingPinus massonianain Yuyao, China. The new species clearly belongs to thehofmannigroup. It is characterised by relatively slim body (a = 43.7 and 36.2 for males and females, respectively), three lines in the lateral field, spicules relatively small (11.4-14.7 μm), mitten-shaped with the posterior third of the dorsal limb distinctly curved, condylus round and slightly dorsally bent, rostrum well developed with more or less rounded terminus, distal ends of spicules not forming a clear cucullus, male tail with a mucron-like process, female tail slightly ventrally bent with a bluntly rounded terminus, vulval lip not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. The separate species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on ITS1/2 sequences, which revealed thatB. yuyaoensisn. sp. is close toB. parvispicularisandB. paraparvispicularis.

Description of Aphelenchoides huntensis sp. n. (Nematoda: Aphelenchoididae) isolated from Pinus sylvestris in western Iran

Nematology ◽  
2016 ◽  
Vol 18 (3) ◽  
pp. 357-366 ◽  
Author(s):  
Mehrab Esmaeili ◽  
Yiwu Fang ◽  
Hongmei Li ◽  
Ramin Heydari
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Pinus Sylvestris ◽  
Excretory Pore ◽  
Western Iran ◽  
Rounded Apex ◽  
Female Tail ◽  
Lateral Lines ◽  
Group 2 ◽  
A New Species

Aphelenchoides huntensissp. n. is described and illustrated from wood and bark samples of a dead scots pine in Kermanshah Province, western Iran. The new species has a body length of 507-673 μm (females) and 636-640 μm (males). The cuticle is weakly annulated with four lateral lines. Lip region offset. The stylet is 9.5 (9.0-10.0) μm long with small basal swellings. The excretory pore is locatedcaone body diam. posterior to the metacorpus valve. The spicules are large with the dorsal limb 37 or 38 μm long (n = 2), and have a prominent rostrum and rounded apex. The female tail is conical, the terminus having a complicated step-like projection, usually with many tiny nodular protuberances. The male tail bears six (2 + 2 + 2) caudal papillae and a well-developed mucron. The new species belongs to the Group 2 category ofAphelenchoidesspecies. Phylogenetic analysis based on the 18S and 28S D2-D3 regions of rDNA confirmed its status as a new species.

Description of Ruehmaphelenchus taedae n. sp. (Nematoda: Aphelenchoididae) found in Loblolly pine logs from the USA

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Loblolly Pine ◽  
Excretory Pore ◽  
Lateral Field ◽  
Female Tail ◽  
The Status ◽  
The Usa ◽  
Males And Females ◽  
A New Species

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.

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