Speciation of the genus Dolichopoda in Anatolia with reference to the role of ancient central lake system

2016 ◽  
Vol 47 (3) ◽  
pp. 267-283
Author(s):  
Mehmet Sait Taylan ◽  
Deniz Şirin
Keyword(s):  
Genetic Diversity ◽  
16S Rdna ◽  
Current Distribution ◽  
Phylogenetic Analyses ◽  
Evolutionary Relationships ◽  
Habitat Types ◽  
16S Rdna Gene ◽  
Lake System ◽  
Mitochondrial 16S Rdna

The aim of this study was to determine of the evolutionary relationships of the TurkishDolichopodaspecies, and to define and clarify the systematics of the group in Anatolia. For this purpose, we have examined some 27 populations of the genusDolichopodafrom Anatolia and adjacent regions (Caucasia and the Greek islands). A total of 532 bp of the mitochondrial 16S rDNA gene was sequenced in order to clarify the phylogenetic relationships of the genus. Five main clades can be distinguished and well-supported by phylogenetic analyses. In these clades, the results obtained from Samos Island and Anatolia are remarkable. In contrast to some previous studies, our results point to an Aegean origin of the AnatolianDolichopodaspecies. According to the mitochondrial 16S rDNA divergence data, the current distribution of the genetic diversity was affected by ancient central lake system, habitat types, Messinian period and Plio-Pleistocene characterized by alternating dry/cold and warm/humid stages.

scholarly journals Mitochondrial 16s rDNA profiling and phylogenetic analysis suggest genetic diversity of ash weevil (Stereonichus fraxini De Geer) in Serbia

Genetika ◽  
2019 ◽  
Vol 51 (2) ◽  
pp. 675-686
Author(s):  
Vladislava Galovic ◽  
Milan Drekic ◽  
Sreten Vasic ◽  
Sinisa Andrasev ◽  
Sasa Pekec ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
16S Rdna ◽  
Phylogenetic Analyses ◽  
Molecular Taxonomy ◽  
Geographic Origin ◽  
Phylogenetic Reconstruction ◽  
Point Mutations ◽  
Molecular Evidence ◽  
Bioinformatic Tools ◽  
Mitochondrial 16S Rdna

This study contributes to knowledge of ash weevil (Stereonychus fraxini De Geer) molecular taxonomy, phylogeny and genetic diversity. Adult and larvae stages of insect were collected from several locations covering northern and central part of Serbia cojoined with homologous sequences with respect to their different geographic origin and hypothesis of their evolutionary relationships. Due to its slow rates of evolution the gene region that covers mitochondrial 16S rDNA, was choice for sequence profiling and phylogenetic reconstruction of ash weevil in correspondence with sequences of related tribes Cionini. Phylogenetic analyses demonstrating clear separation of the native weevil populations and the Cionini tribes. Even though bioinformatic tools confirm that all native specimens belong to species Stereonychus fraxini, different profile of the mitochondrial 16S rDNA in the clade of Serbian specimens indicate intraspecific genomic rearrangement in one specimen detached it to northern geographic position. Those particular specimens invade also different Fraxinus species. Genetic distinctness of other imagos from this particular individual proved by indels and point mutations found in their sequences. By screening the mitochondrial 16S rDNA, molecular evidence suggests the existence of the specimen with rearranged genome that indicate genetic variability in native populations of ash weevil species.

High genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus: a contribution to understanding potyvirus evolution

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 243-255 ◽  
Author(s):  
M. Bousalem ◽  
E. J. P. Douzery ◽  
D. Fargette
Keyword(s):  
Genetic Diversity ◽  
Mosaic Virus ◽  
Statistical Tests ◽  
Phylogenetic Analyses ◽  
Natural Populations ◽  
High Genetic Diversity ◽  
Single Clade ◽  
Cultivated Species ◽  
Selection Of

To evaluate the genetic diversity and understand the evolution of Yam mosaic virus (YMV), a highly destructive pathogen of yam (Dioscorea sp.), sequencing was carried out of the C-terminal part of the replicase (NIb), the coat protein (CP) and the 3′-untranslated region (3′-UTR) of 27 YMV isolates collected from the three main cultivated species (Dioscorea alata, the complex Dioscorea cayenensis–Dioscorea rotundata and Dioscorea trifida). YMV showed the most variable CP relative to eight other potyviruses. This high variability was structured into nine distant molecular groups, as revealed by phylogenetic analyses and validated by assessment of the molecular evolutionary noise. No correlation was observed between the CP and 3′-UTR diversities and phylogenies. The most diversified and divergent groups included isolates from Africa. The remaining groups clustered in a single clade and a geographical distinction between isolates from the Caribbean, South America and Africa was observed. The role of the host in the selection of particular isolates was illustrated by the case of a divergent cultivar from Burkina Faso. Phylogenetic topological incongruence and complementary statistical tests highlighted the fact that recombination events, with single and multiple crossover sites, largely contributed to the evolution of YMV. We hypothesise an African origin of YMV from the yam complex D. cayenensis–D. rotundata, followed by independent transfers to D. alata and D. trifida during virus evolution.

scholarly journals Phylogenetics of Hydroidolina (Hydrozoa: Cnidaria)

2008 ◽  
Vol 88 (8) ◽  
pp. 1663-1672 ◽  
Author(s):  
Paulyn Cartwright ◽  
Nathaniel M. Evans ◽  
Casey W. Dunn ◽  
Antonio C. Marques ◽  
Maria Pia Miglietta ◽  
...  
Keyword(s):  
16S Rdna ◽  
Dna Sequences ◽  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Sensu Stricto ◽  
28S Rdna ◽  
Mitochondrial 16S Rdna

Hydroidolina is a group of hydrozoans that includes Anthoathecata, Leptothecata and Siphonophorae. Previous phylogenetic analyses show strong support for Hydroidolina monophyly, but the relationships between and within its subgroups remain uncertain. In an effort to further clarify hydroidolinan relationships, we performed phylogenetic analyses on 97 hydroidolinan taxa, using DNA sequences from partial mitochondrial 16S rDNA, nearly complete nuclear 18S rDNA and nearly complete nuclear 28S rDNA. Our findings are consistent with previous analyses that support monophyly of Siphonophorae and Leptothecata and do not support monophyly of Anthoathecata nor its component subgroups, Filifera and Capitata. Instead, within Anthoathecata, we find support for four separate filiferan clades and two separate capitate clades (Aplanulata and Capitatasensu stricto). Our data however, lack any substantive support for discerning relationships between these eight distinct hydroidolinan clades.

scholarly journals Construction of a DNA database for ticks collected in Japan: application of molecular identification based on the mitochondrial 16S rDNA gene

2014 ◽  
Vol 65 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Ai TAKANO ◽  
Hiromi FUJITA ◽  
Teruki KADOSAKA ◽  
Mamoru TAKAHASHI ◽  
Takeo YAMAUCHI ◽  
...  
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Dna Database ◽  
Mitochondrial 16S Rdna

scholarly journals Characterization of a ‘Bacteroidetes’ symbiont in Encarsia wasps (Hymenoptera: Aphelinidae): proposal of ‘Candidatus Cardinium hertigii’

2004 ◽  
Vol 54 (3) ◽  
pp. 961-968 ◽  
Author(s):  
Einat Zchori-Fein ◽  
Steve J. Perlman ◽  
Suzanne E. Kelly ◽  
Nurit Katzir ◽  
Martha S. Hunter
Keyword(s):  
Electron Microscopy ◽  
16S Rdna ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
16S Rdna Sequence ◽  
Rdna Sequences ◽  
16S Rdna Sequences

Previously, analysis of 16S rDNA sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the ‘Bacteroidetes’. This symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several Encarsia parasitoid wasps (Hymenoptera: Aphelinidae). In this study, electron microscopy and phylogenetic analysis of the 16S rRNA and gyrB genes of symbionts from Encarsia hispida and Encarsia pergandiella are used to describe and further characterize these bacteria. Phylogenetic analyses based on these two genes showed that the Encarsia symbionts are allied with the Cytophaga aurantiaca lineage within the ‘Bacteroidetes’, with their closest described relative being the acanthamoeba symbiont ‘Candidatus Amoebophilus asiaticus’. The Encarsia symbionts share 97 % 16S rDNA sequence similarity with Brevipalpus mite and Ixodes tick symbionts and 88 % sequence similarity with ‘Candidatus A. asiaticus’. Electron microscopy revealed that many of the bacteria found in the ovaries of the two Encarsia species contained a regular, brush-like array of microfilament-like structures that appear to be characteristic of the symbiont. Finally, the role of this bacterium in parthenogenesis induction in E. hispida was confirmed. Based on phylogenetic analyses and electron microscopy, classification of the symbionts from Encarsia as ‘Candidatus Cardinium hertigii’ is proposed.

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