Molecular phylogeny and phylogeography of Holarctic species of Pluteus section Pluteus (Agaricales: Pluteaceae), with description of twelve new species

Phytotaxa ◽  
2014 ◽  
Vol 180 (1) ◽  
pp. 1 ◽  
Author(s):  
Alfredo Justo ◽  
Ekaterina Malysheva ◽  
Tatiana Bulyonkova ◽  
Else C Vellinga ◽  
Gerry Cobian ◽  
...  
Keyword(s):  
Genetic Variation ◽  
New Species ◽  
Molecular Phylogeny ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Western Palearctic ◽  
Holarctic Region ◽  
Morphological And Molecular Data ◽  
Better Than ◽  
Holarctic Species

The taxonomy and phylogeography of Pluteus section Pluteus in the Holarctic region was investigated using morphological and molecular data. Over 300 specimens spanning the major areas of boreal and temperate forests of the Northern Hemisphere were studied and nrITS and tef1 were obtained for phylogenetic analyses. In order to stabilize the taxonomy of the group all available type collections were studied and, if possible, sequenced. A total of 26 species occurring in Eurasia and North America were recovered in the phylogenetic analyses. Twelve species are described as new (Pluteus rangifer, P. elaphinus, P. hibbettii, P. eos, P. orestes, P. methvenii, P. shikae, P. kovalenkoi, P. leucoborealis, P. sepiicolor, P. oreibatus, P. atrofibrillosus), one is provisionally named (P. parilis) and one variety is raised to species rank (P. americanus). In many cases separation of the species based on morphology alone is challenging. In general, tef1 distinguishes the species better than nrITS. Structured infraspecific genetic variation was detected in the nrITS phylogenies for five species (P. atromarginatus, P. hibbettii, P. orestes, P. primus and P. shikae) and in the tef1 phylogenies for P. cervinus. Phylogeographic patterns are strikingly different among the species in this group and include widespread Holarctic species, exclusively Palearctic, putative disjuncts and endemics in each Holarctic subregion (Eastern/Western Palearctic and Nearctic). Identification keys are provided for each subregion.

scholarly journals Loose Ends in the Cortinarius Phylogeny: Five New Myxotelamonoid Species Indicate a High Diversity of These Ectomycorrhizal Fungi with South American Nothofagaceae

Life ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 420
Author(s):  
María Eugenia Salgado Salomón ◽  
Carolina Barroetaveña ◽  
Tuula Niskanen ◽  
Kare Liimatainen ◽  
Matthew E. Smith ◽  
...  
Keyword(s):  
New Species ◽  
Current Knowledge ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Global Scale ◽  
South American ◽  
Edible Fungi ◽  
Rdna Its ◽  
Morphological And Molecular Data ◽  
High Diversity

This paper is a contribution to the current knowledge of taxonomy, ecology and distribution of South American Cortinarius (Pers.) Gray. Cortinarius is among the most widely distributed and species-rich basidiomycete genera occurring with South American Nothofagaceae and species are found in many distinct habitats, including shrublands and forests. Due to their ectomycorrhizal role, Cortinarius species are critical for nutrient cycling in forests, especially at higher latitudes. Some species have also been reported as edible fungi with high nutritional quality. Our aim is to unravel the taxonomy of selected Cortinarius belonging to phlegmacioid and myxotelamonioid species based on morphological and molecular data. After widely sampling Cortinarius specimens in Patagonian Nothofagaceae forests and comparing them to reference collections (including holotypes), we propose five new species of Cortinarius in this work. Phylogenetic analyses of concatenated rDNA ITS-LSU and RPB1 sequences failed to place these new species into known Cortinarius sections or lineages. These findings highlight our knowledge gaps regarding the fungal diversity of South American Nothofagaceae forests. Due to the high diversity of endemic Patagonian taxa, it is clear that the South American Cortinarius diversity needs to be discovered and described in order to understand the evolutionary history of Cortinarius on a global scale.

scholarly journals Insights on The Morphological and Phylogenetic Delimitation of Dacrymyces Nees

2021 ◽  
Author(s):  
Renato Lúcio Mendes Alvarenga ◽  
Tatiana Baptista Gibertoni
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Morphological Features ◽  
New Combinations ◽  
Dna Analyses ◽  
Homogeneous Composition ◽  
Morphological And Molecular Data

Abstract Dacrymycetes has four families and 13 genera, few of them with molecular data available and then usually polyphyletic in phylogenetic analyses. Dacrymyces Nees is one of the polyphyletic genera in Dacrymycetes and it was introduced to accommodate one species, D. stillatus Nees. The morphological features of the genus are a homogeneous composition of the intra-structure and an amphigenous or superior hymenium. In this study, we included Neotropical specimens in the phylogeny of the Dacrymycetes and Dacrymyces s.s. is emended to include species with resupinate basidiomata, unilateral hymenium and heterogeneous context. In this new delimitation, the new species Dacrymyces flavobrunneus is described using morphological and molecular data and three new combinations (D. ceraceus comb. nov., D. maxidorii comb. nov. and D. spathularia comb. nov.) are proposed based on DNA analyses.

Cymbidium xichouense (Orchidaceae; Epidendroideae), a new species from China: evidence from morphological and molecular data

Phytotaxa ◽  
2021 ◽  
Vol 484 (3) ◽  
pp. 291-297
Author(s):  
XINYU XU ◽  
CHANG-CHUN DING ◽  
WENQI HU ◽  
XIA YU ◽  
YU ZHENG ◽  
...  
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Phylogenetic Analyses ◽  
Plastid Dna ◽  
Molecular Data ◽  
Morphological Evidence ◽  
The Status ◽  
Nuclear Its ◽  
Morphological And Molecular Data ◽  
A New Species

A new species of Cymbidium (Orchidaceae), Cymbidium xichouense, from Yunnan Province, China, is described and illustrated based on morphological evidence and molecular analyses. The new orchid is morphologically similar to C. qinbeiense, but it has several morphological features that distinguish it from C. qiubeinense and all other recognized species in Cymbidium. Phylogenetic analyses based on nuclear (ITS) and plastid DNA (matK) were conducted, and the results also supported the status of C. xichouense as a new species, which is sister to C. qiubeiense.

Molecular phylogeny of the weevil genus Kyklioacalles Stüben, with descriptions of a new subgenus Glaberacalles and two new species (Curculionidae: Cryptorhynchinae)

Zootaxa ◽  
2010 ◽  
Vol 2662 (1) ◽  
pp. 28 ◽  
Author(s):  
PETER E. STÜBEN ◽  
JONAS J. ASTRIN
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
Bayesian Analysis ◽  
Morphological Differentiation ◽  
Molecular Data ◽  
Mitochondrial Genes ◽  
Morphological Evidence ◽  
New Subgenus ◽  
Western Palearctic ◽  
Two New Species

A molecular phylogeny of the western Palearctic weevil genus Kyklioacalles Stüben, 1999 is presented, combining two mitochondrial genes (CO1 and 16S) in a Bayesian analysis. Based on molecular data, the validity of the subspecies Kyklioacalles punctaticollis punctaticollis (Lucas, 1849) and Kyklioacalles punctaticollis meteoricus (Meyer, 1909) is discussed and the morphological differentiation of the endophalli and known distributions of both subspecies are verified. Glaberacalles subg. n. (formerly Kyklioacalles punctaticollis-group) and two new species are described, Kyklioacalles atlasicus sp.n. from Morocco and Kyklioacalles plantapilosus sp.n. from Spain. Kyklioacalles berberi (Stüben, 2005), comb. n. and Kyklioacalles olcesei (Tournier, 1873) comb. n. are transferred from Acalles Schoenherr. The molecular results further advocate a transfer of Onyxacalles pyrenaeus (Boheman, 1844) to Kyklioacalles; however this is not supported by morphological evidence. Kyklioacalles almadensis Stüben, 2004 syn. n. (Spain) is synonymized with Kyklioacalles bupleuri Stüben, 2004 (Tunisia). A catalogue of all 40 (sub-)species of Kyklioacalles is given and a key of the species of the subgenus Glaberacalles is presented.

A new species of the Ranunculaceae from southwestern China based on morphological and molecular data

Phytotaxa ◽  
2016 ◽  
Vol 283 (1) ◽  
pp. 43 ◽  
Author(s):  
BING LIU ◽  
YUN-QING HAO ◽  
YONG YANG ◽  
TIAN-TIAN SUN ◽  
YANG-JUN LAI ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Conservation Status ◽  
Molecular Data ◽  
Southwestern China ◽  
Leaf Margin ◽  
Molecular Systematic ◽  
Field Investigations ◽  
Morphological And Molecular Data ◽  
A New Species

Based on field investigations, morphological and molecular systematic studies, a new species, Caltha dysosmoides (Ranunculaceae) from southwestern China is described. It differs from all other known congeneric species by the densely dentate leaf margin, the pendulous pedicels, the scarlet flowers, the compressed filaments, and the triangular connective. To better understand taxonomy of this new species, phylogenetic analyses were conducted using a combined dataset from nrITS, plastid trnL-trnF region, and atpB-rbcL spacer. The results indicate that Caltha is monophyletic and C. dysosmoides is sister to C. sinogracilis. The conservation status of the new species is categorized as CR based on IUCN criteria.

scholarly journals Two new species of snail-eating snakes of the genus Tropidodipsas (Serpentes, Dipsadidae) from southern Mexico, with notes on related species

Herpetozoa ◽  
2021 ◽  
Vol 34 ◽  
pp. 233-257
Author(s):  
Christoph I. Grünwald ◽  
Sarahi Toribio-Jiménez ◽  
Carlos Montaño-Ruvalcaba ◽  
Hector Franz-Chávez ◽  
Miguel A. Peñaloza-Montaño ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
Molecular Data ◽  
Species Group ◽  
Conservation Priorities ◽  
Southern Mexico ◽  
Two New Species ◽  
Taxonomic Changes ◽  
Morphological And Molecular Data ◽  
Mexican Species

We describe two new species of Tropidodipsas related to the T. fasciata species group as defined by Kofron (1987), and provide morphological and molecular data to support the novelty of both species. A partial molecular phylogeny of the Mexican species of snail-eating snakes (Serpentes, Dipsadidae) is presented, and we discuss evolutionary relationships as supported by our molecular results. We analyze specific relationships of the new species described herein with their closest relatives. We present a distribution map for all species of Tropidodipsas and include photographs of living individuals of each species. Finally, we discuss other taxonomic changes based on our molecular phylogeny as well as conservation priorities of the new species.

Description of a new species of Dicyphus Fieber (Insecta: Heteroptera: Miridae) from Portugal based on morphological and molecular data

2006 ◽  
Vol 37 (3) ◽  
pp. 281-300 ◽  
Author(s):  
Gerasimos Cassis ◽  
Jose Isidro Martinez-Cascales ◽  
Juan Antonio Sanchez
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Molecular Data ◽  
Species Identity ◽  
Nesidiocoris Tenuis ◽  
Dicyphus Tamaninii ◽  
Morphological And Molecular Data ◽  
Similar Pair ◽  
A New Species

AbstractA new species of the plantbug genus Dicyphus (Insecta: Heteroptera: Miridae), D. umbertae Sanchez & Cassis sp. n., from Portugal is described and the closely related species, Dicyphus cerastii Wagner is redescribed. Several body measurements were taken. Male and female genitalia of both species are illustrated. D. umbertae was found in several localities in Central and South of Portugal on tomato (Lycopersicon esculentum Mill.) and Hyosciamus albus L. D. cerastii was found in southeast Spain on squash (Cucurbita maxima Duchesne), L. esculentum, Ononis natrix L., Withania frutescens (L.) Pauquy and H. albus. The external morphology of the two species is very alike and measurements overlap. In the two species the aedeagus has a similar pair of prominent slightly arcuate sclerites, but the shape and setae on the left paramere are different. Phylogenetic analyses were performed using 381 bp fragments of Cytochrome b. Other dicyphine species were used as outgroups in the analyses: Dicyphus tamaninii Wagner, D. rubicundus Blöte, D. escalerae Lindberg, Nesidiocoris tenuis (Reuter), Cyrtopeltis geniculata Fieber and Macrolophus sp. Several evolutionary models were explored under a maximum likelihood framework. The molecular analyses provided strong support for the species identity of the two sister species D. umbertae and D. cerastii.

Cymbidium motuoense (Orchidaceae; Epidendroideae), a new species from China: evidence from morphological and molecular data

Phytotaxa ◽  
2021 ◽  
Vol 509 (1) ◽  
Author(s):  
WENQI HU ◽  
QINGHAI ZHANG ◽  
GUIZHEN CHEN ◽  
MENGJIA ZHU ◽  
XIA YU ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Plastid Dna ◽  
Molecular Data ◽  
Natural Hybrid ◽  
Closely Related Species ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Morphological And Molecular Data ◽  
A New Species

This study describes a new orchid species, Cymbidium motuoense, from Xizang, China based on morphological and molecular analyses. Several unique morphological features distinguish this new orchid from all other species of Cymbidium. Based on its morphology, this orchid is similar to C. tracyanum but differs in its yellow-green flower with dark purple-red spots on the veins, oblong-elliptic sepals, side lobes of the lip with reddish brown hairs along the veins and disc with three purple-red lamellae. Phylogenetic analyses based on plastid DNA (matK and rbcL) supported C. motuoense as a new species. However, the low support in nuclear ribosomal ITS tree provide the possibility that the new orchid may be a natural hybrid of C. tracyanum and C. iridioides or another closely related species.

scholarly journals Integrative Taxonomy, Phylogeny, and New Species of the Weevil GenusOnyxacallesStüben (Coleoptera: Curculionidae: Cryptorhynchinae)

2012 ◽  
Vol 2012 ◽  
pp. 1-22
Author(s):  
Peter E. Stüben ◽  
Jonas J. Astrin
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
Related Species ◽  
Mediterranean Area ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Mitochondrial Genes ◽  
Western Palearctic ◽  
Species Groups ◽  
Three New Species

A molecular phylogeny of the western Palearctic weevil genusOnyxacallesStüben, 1999 is presented, combining two mitochondrial genes (COI and 16S) in a Bayesian analysis. Based on molecular data,Onyxacalles pyrenaeusBoheman, 1844 is transferred into the genusKyklioacallesStüben 1999 (K. faustigroup) and—in an integrative taxonomy framework—the interaction between morphology and molecular analysis is illustrated. The species ofOnyxacalless. str. are assigned to three new species groups,O. henoni,O. luigionii, andO. portusvenerisgroups. The distribution of the related species in the Mediterranean area is illustrated with values of COI and 16S p-distances. Three new species are described and distinguished from their related species:Onyxacalles nuraghiStüben sp.n. from Italy (Sardinia),Onyxacalles torreStüben and Astrin sp. n. from France (Corsica) andOnyxacalles vilaeStüben sp. n. from Croatia (Velebit Mts.). A catalogue of all 20 species ofOnyxacallesis given, and a key is finally presented combined with image stacking of the habitus and aedeagus for all species.

scholarly journals Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446

MycoKeys ◽  
2020 ◽  
Vol 63 ◽  
pp. 163-172
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

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