Three new species of Calocybella from India based on morphology and molecular phylogeny

Phytotaxa ◽  
2016 ◽  
Vol 255 (2) ◽  
pp. 133 ◽  
Author(s):  
K. P. DEEPNA LATHA ◽  
K. N. ANIL RAJ ◽  
THUSHARA CHEROLIL ◽  
SHAHINA A. SHARAFUDHEEN ◽  
PATINJAREVEETTIL MANIMOHAN

Three new species of Calocybella, C. lohitha, C. babruka and C. swetha are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions of these species are provided along with photo-illustrations and comparisons with phenetically similar and phylogenetically related species. Nuclear ribosomal internal transcribed spacer (ITS) region of the three species were sequenced and analyzed. In addition, nuclear ribosomal large subunit (nrLSU) region of Calocybella swetha was sequenced and used in BLASTn search to find similar sequences. ITS-based phylogenetic analysis using Maximum likelihood (ML) method supported both the novelty of these species and their placement within the genus. The discovery of these three species forms the first record of Calocybella outside Europe.

Phytotaxa ◽  
2016 ◽  
Vol 246 (1) ◽  
pp. 61 ◽  
Author(s):  
K. P. Deepna Latha ◽  
K. N. Anil Raj ◽  
V. Adnaan Farook ◽  
Shahina A. Sharafudheen ◽  
Neeraja K. Parambil ◽  
...  

Three new species of Russulaceae, Lactifluus umbonatus and Lactifluus indicus belonging to Lf. subg. Gerardii and Lactarius keralensis belonging to L. subg. Piperites, are described from Kerala State, India. Comprehensive descriptions, photographs, line drawings and comparisons with phenetically similar and phylogenetically related species are given. Nuclear ribosomal internal transcribed spacer regions (ITS) of the three species were sequenced and analyzed. In addition, nuclear ribosomal large subunit (nLSU) gene of Lactifluus indicus was sequenced and the sequence was used in BLASTn search to find similar sequences. Maximum likelihood (ML) analysis based on the ITS sequences confirmed both the novelty of these species and their placement within their respective genera. The discovery of Lactifluus umbonatus and Lf. indicus represent the first report of novel species belonging to Lf. subg. Gerardii from India. Lactifluus indicus represents the second record of a pleurotoid Lactifluus species from India.


Phytotaxa ◽  
2016 ◽  
Vol 286 (4) ◽  
pp. 232 ◽  
Author(s):  
K. N.ANIL RAJ ◽  
PATINJAREVEETTIL MANIMOHAN

Three new species of Entoloma belonging to the subgenus Nolanea, E. brunneoumbonatum, E. luteodiscum and E. brunneoapplanatum, are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions, photographs, and comparisons with phenetically similar and phylogenetically related species are discussed. The nuclear ribosomal internal transcribed spacer region (ITS) of the three species was sequenced and analyzed. The ITS-based phylogenetic analysis, performed using Maximum Likelihood (ML) method, supported both the novelty of these species and their infrageneric placement within the subgenus Nolanea. A key to the ten species of Entoloma subgenus Nolanea known from India is also provided.


MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 103-139
Author(s):  
Guo-Jie Li ◽  
Shou-Mian Li ◽  
Bart Buyck ◽  
Shi-Yi Zhao ◽  
Xue-Jiao Xie ◽  
...  

Three new species of Russulasection Ingratae, found in Guizhou and Jiangsu Provinces, southern China, are proposed: R. straminella, R. subpectinatoides and R. succinea. Photographs, line drawings and detailed morphological descriptions for these species are provided with comparisons against closely-related taxa. Phylogenetic analysis of the internal transcribed spacer (ITS) region supported the recognition of these specimens as new species. Additionally, R. indocatillus is reported for the first time from China and morphological and phylogenetic data are provided for the Chinese specimens.


Phytotaxa ◽  
2019 ◽  
Vol 397 (1) ◽  
pp. 23
Author(s):  
WEI SUN ◽  
BINGJIE LIU ◽  
RONG FU ◽  
XINGZHONG LIU ◽  
MEICHUN XIANG

During survey on rock-inhabiting fungi from karst habitats in Guizhou, China, two new species in Cyphellophora were discovered and identified. Phylogenetic analysis based on combined sequences of the nuclear large subunit (nucLSU) and internal transcribed spacer (ITS) region of ribosomal DNA revealed that the tested isolates, clustered into two clades that well affiliated in the genus of Cyphellophora. Morphological characteristics were also supported the estabolishment of the new species. Herewith Cyphellophora botryose sp. nov. and Cyphellophora guizhouensis sp. nov. were described and their differences from closely related species were discussed.


Phytotaxa ◽  
2015 ◽  
Vol 221 (2) ◽  
pp. 166 ◽  
Author(s):  
K. P. Deepna Latha ◽  
Patinjareveettil Manimohan

Inocybe griseorubida sp. nov. is described from Kerala State, India. A comprehensive description, photographs, and comparisons with phenetically similar species are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (rpb2) gene of this species were sequenced and analyzed. Phylogenetic analysis of rpb2 sequences confirmed both the novelty of the species and its placement within the Pseudosperma clade.


Phytotaxa ◽  
2016 ◽  
Vol 267 (1) ◽  
pp. 40 ◽  
Author(s):  
K. P. DEEPNA LATHA ◽  
PATINJAREVEETTIL MANIMOHAN ◽  
P. BRANDON MATHENY

Inocybe distincta sp. nov. is described from Kerala State, India. A comprehensive description, photographs, line drawings and comments are provided. The nuclear ribosomal internal transcribed spacer region (ITS), a portion of the nuclear ribosomal large subunit (nrLSU) and a portion of the nuclear second-largest subunit of RNA polymerase II (RPB2) gene of this species were sequenced and analyzed. BLASTn searches using nrLSU and RPB2 sequences and subsequent ML phylogenetic analysis of combined nrLSU and RPB2 sequences confirmed that Inocybe distincta is a representative of the Nothocybe lineage. As the Nothocybe lineage is assumed to have affinities to I. cutifracta, and as there are different interpretations of that species, we examined the holotype of I. cutifracta collected by T. Petch and another collection from Sri Lanka identified as I. cutifracta by D. N. Pegler, and we present here our observations on these collections.


Phytotaxa ◽  
2016 ◽  
Vol 278 (3) ◽  
pp. 287 ◽  
Author(s):  
K. P. DEEPNA LATHA ◽  
NEERAJA K. Paramban ◽  
PATINJAREVEETTIL MANIMOHAN

Tubaria keralensis sp. nov. is described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive description, photographs, and comparisons with phenetically similar and phylogenetically related species are provided. Tubaria keralensis is characterized by its strongly intervenose lamellae with concolorous edges; a stipe with velar remnants and a white, strigose basal mycelium and broadly ellipsoid to ovoid basidiospores. Nuclear ribosomal internal transcribed spacer region (nrITS) and nuclear ribosomal large subunit (nrLSU) gene region were sequenced and analyzed. Maximum likelihood (ML) analysis of combined nrITS and nrLSU sequence data revealed the novelty of the species as well as its placement in the T. furfuracea complex. The present discovery of T. keralensis forms the second report of a new Tubaria species from Kerala.


Phytotaxa ◽  
2018 ◽  
Vol 343 (1) ◽  
pp. 47 ◽  
Author(s):  
K. N. ANIL RAJ ◽  
PATINJAREVEETTIL MANIMOHAN

Three species of Clitopilus, C. albidus sp. nov., C. subscyphoides and C. orientalis, are described from Kerala State, India, based on morphology and molecular phylogeny. Comprehensive descriptions, photographs, and comparisons with phenetically similar and phylogenetically related species are provided. The RNA polymerase II genes (rpb2) of the three species were sequenced and analyzed. In addition, the nuclear ribosomal internal transcribed spacer regions (ITS) and the nuclear ribosomal large subunit (nLSU) genes were sequenced and used in BLAST searches to find similar sequences. Phylogenetic analyses based on rpb2 sequences performed using both Maximum Likelihood (ML) and Bayesian Analysis (BA) methods supported the novelty of C. albidus and its placement within the genus Clitopilus. C. subscyphoides and C. orientalis are the first and second record respectively of these species from India.


Phytotaxa ◽  
2017 ◽  
Vol 298 (1) ◽  
pp. 20 ◽  
Author(s):  
YUAN YUAN ◽  
XIAO-HONG JI ◽  
FANG WU ◽  
JIA-JIA CHEN

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.


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