Three new host records of endophytic Neofusicoccum species reported from Dendrobium orchid

Neofusicoccum species are endophytes, saprobes and opportunistic pathogens of many economic and ornamental plants. There are few reports of Neofusicoccum species are available to be associated with orchids worldwide. In this study, five endophytic Neofusicoccum strains were isolated from Dendrobium orchid leaves and stems in southwestern China and northern Thailand. Morphological characteristics and phylogenetic analysis of the combined partial nuclear rDNA internal transcribed spacer (ITS), partial RNA polymerase Ⅱ second largest subunit (RPB2), part of the translation elongation factor 1 alpha (EF-1α) and beta-tubulin (TUB2) dataset revealed that these isolates belong to two species Neofusicoccum occulatum and N. parvum. This is the first record of Neofusicoccum occulatum associated with Dendrobium chrysanthum while N. parvum associated with D. harveyanum and D. moschatum.

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One New Species and Two New Host Records of Apiospora from Bamboo and Maize in Northern Thailand with Thirteen New Combinations

Life ◽

10.3390/life11101071 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1071

Author(s):

Xingguo Tian ◽

Samantha C. Karunarathna ◽

Ausana Mapook ◽

Itthayakorn Promputtha ◽

Jianchu Xu ◽

...

Keyword(s):

Sequence Data ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

New Combinations ◽

New Host ◽

Translation Elongation ◽

Northern Thailand ◽

Dna Sequence Data ◽

New Host Records

The genus Apiospora is known as a cosmopolitan genus, found across various substrates. In this study, four Apiospora taxa were obtained from the decaying stems of bamboo and maize in northern Thailand. Apiospora collections were compared with known species based on the morphological characteristics and the DNA sequence data of internal transcribed spacer (ITS), the partial large subunit nuclear rDNA (LSU), the translation elongation factor 1-alpha gene (TEF1-α) and beta-tubulins (TUB2). Apiospora chiangraiense sp. nov. and two new host records (Ap. intestini and Ap. rasikravindra) are introduced here based on the morphological characteristics and multi-locus analyses. Additionally, thirteen species previously identified as Arthrinium are introduced as new combinations in Apiospora, viz., Ap. acutiapica, Ap. bambusicola, Ap. biserialis, Ap. cordylines, Ap. cyclobalanopsidis, Ap. euphorbiae, Ap. gelatinosa, Ap. locuta-pollinis, Ap. minutispora, Ap. pseudorasikravindrae, Ap. septate, Ap. setariae and Ap. sorghi.

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Bambusicolous Arthrinium Species in Guangdong Province, China

Frontiers in Microbiology ◽

10.3389/fmicb.2020.602773 ◽

2020 ◽

Vol 11 ◽

Author(s):

Indunil C. Senanayake ◽

Jayarama D. Bhat ◽

Ratchadawan Cheewangkoon ◽

Ning Xie

Keyword(s):

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Guangdong Province ◽

Nuclear Ribosomal Dna ◽

Internal Transcribed Spacer Region ◽

Translation Elongation ◽

Bambusicolous Fungi ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

A survey of bambusicolous fungi in Bijiashan Mountain Park, Shenzhen, Guangdong Province, China, revealed several Arthrinium-like taxa from dead sheaths, twigs, and clumps of Bambusa species. Phylogenetic relationships were investigated based on morphology and combined analyses of the internal transcribed spacer region (ITS), large subunit nuclear ribosomal DNA (LSU), beta tubulin (β-tubulin), and translation elongation factor 1-alpha (tef 1-α) gene sequences. Based on morphological characteristics and phylogenetic data, Arthrinium acutiapicum sp. nov. and Arthrinium pseudorasikravindrae sp. nov. are introduced herein with descriptions and illustrations. Additionally, two new locality records of Arthrinium bambusae and Arthrinium guizhouense are described and illustrated.

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Two new Lycoperdon species collected from Korea: L. albiperidium and L. subperlatum spp. nov.

Phytotaxa ◽

10.11646/phytotaxa.260.2.1 ◽

2016 ◽

Vol 260 (2) ◽

pp. 101 ◽

Cited By ~ 1

Author(s):

CHANG SUN KIM ◽

JONG WON JO ◽

YOUNG-NAM KWAG ◽

GI-HO SUNG ◽

JAE-GU HAN ◽

...

Keyword(s):

Sequence Data ◽

Elongation Factor ◽

Morphological Characteristics ◽

Its Region ◽

Its Sequences ◽

Translation Elongation ◽

Protein Coding ◽

Dna Sequence Data ◽

Coding Regions ◽

Elongation Factor 1 Alpha

Thirty-four Lycoperdon specimens from Korea were examined with the internal transcribed spacer (ITS) region of ribosomal DNA sequence data. The result of the ITS sequences phylogenetic analysis indicated that the Korean specimens represented nine different species. To confirm the taxonomic position of these species, we conducted an intensive morphological investigation, and additional phylogenetic investigation of the protein coding regions RNA polymerase subunit II (RPB2) and translation elongation factor 1-alpha (TEF1). We discovered two new species (L. albiperidium and L. subperlatum) and one (L. ericaeum) newly discovered in Korea. Lycoperdon albiperidium is closely related to L. ericaeum based on ITS, RPB2 and TEF1 sequence data, but these species were distinguishable by morphological characteristics, especially the shape of the basidiocarps, the diameter of the eucapillitial threads and the size of the basidospores. Lycoperdon subperlatum is quite similar to the European and American L. perlatum based on morphological characteristics. However, L. subperlatum is clearly distinct from European and American L. perlatum based on ITS, RPB2 and TEF1 sequence data, and somewhat differs from them in macro- and microscopic characteristics. Based on morphological characteristics, L. ericaeum is related to L. subumbrinum and L. lividum but it is distinguishable by the presence of fragile, eucapillitial threads, the diameters of the threads and ITS sequences. Here, we describe four Lycoperdon species collected in Korea.

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Redefining genera of cereal pathogens: Oculimacula, Rhynchosporium and Spermospora

Fungal Systematics and Evolution ◽

10.3114/fuse.2021.07.04 ◽

2020 ◽

Author(s):

P.W. Crous ◽

U. Braun ◽

B.A. McDonald ◽

C.L. Lennox ◽

J. Edwards ◽

...

Keyword(s):

Rna Polymerase Ii ◽

Dna Sequences ◽

Large Subunit ◽

Elongation Factor ◽

Morphological Characteristics ◽

Minor Importance ◽

Ammophila Arenaria ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Three New Species

The taxonomy of Oculimacula, Rhynchosporium and Spermospora is re-evaluated, along with that of phylogenetically related genera. Isolates are identified using comparisons of DNA sequences of the internal transcribed spacer ribosomal RNA locus (ITS), partial translation elongation factor 1-alpha (tef1), actin (act), DNA-directed RNA polymerase II largest (rpb1) and second largest subunit (rpb2) genes, and the nuclear ribosomal large subunit (LSU), combined with their morphological characteristics. Oculimacula is restricted to two species, O. acuformis and O. yallundae, with O. aestiva placed in Cyphellophora, and O. anguioides accommodated in a new genus, Helgardiomyces. Rhynchosporium s. str. is restricted to species with 1-septate conidia and hooked apical beaks, while Rhynchobrunnera is introduced for species with 1–3-septate, straight conidia, lacking any apical beak. Rhynchosporium graminicola is proposed to replace the name R. commune applied to the barley scald pathogen based on nomenclatural priority. Spermospora is shown to be paraphyletic, representing Spermospora (type: S. subulata), with three new species, S. arrhenatheri, S. loliiphila and S. zeae, and Neospermospora gen. nov. (type: N. avenae). Ypsilina (type: Y. graminea), is shown to be monophyletic, but appears to be of minor importance on cereals. Finally, Vanderaaea gen. nov. (type: V. ammophilae), is introduced as a new coelomycetous fungus occurring on dead leaves of Ammophila arenaria.

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Five New Species of Trichoderma From Wetland Soils in China

10.21203/rs.3.rs-516164/v1 ◽

2021 ◽

Author(s):

Guang Zhi Zhang ◽

HeTong Yang ◽

Xin Jian Zhang ◽

Fang Yuan Zhou ◽

Xiao-Qing Wu ◽

...

Keyword(s):

Rna Polymerase Ii ◽

Phylogenetic Analyses ◽

Elongation Factor ◽

Morphological Characteristics ◽

Aerial Mycelium ◽

Wetland Soils ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Inner Mongolia Autonomous Region ◽

Subunit B

Abstract Trichoderma isolates were collected from wetland soils in different areas of China. Combined analyses of morphological characteristics and phylogenetic analyses by partial translation elongation factor 1 alpha (TEF1-α) and RNA polymerase II subunit b (RPB2) revealed five new Trichoderma species, namely, Trichoderma macrofasciculatum, T. shangrilaense, T. nordicum, T. vadicola, and T. hailarense. T. macrofasciculatum and T. shangrilaense belonging to the Polysporum Clade were isolated from wetland soils collected from Sichuan and Yunnan Provinces. The conidiation of T. macrofasciculatum typically appeared in white pustules in concentric rings on PDA or MEA, and its conidia had two or more guttules. Conidiation of T. shangrilaense formed white pustules with irregular shape and size, and its conidia were mostly obovoid and smooth. Trichoderma vadicola, T. nordicum, and T. hailarense belonging to the Viride Clade were collected from Shandong Province, Beijing Municipality, and Inner Mongolia Autonomous Region, respectively. The phialides of T. nordicum lageniform were curved on PDA, and its conidia were globose to obovoidal and large. The aerial mycelium of T. vadicola formed strands and floccose mat. The colonies of T. hailarense cannot form conidia on PDA, and the conidia of T. hailarense on other media were obovoid and delicately roughened.

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Identification of the fungal endophyte of Ammophila breviligulata (American beachgrass) as Epichloë amarillans

PeerJ ◽

10.7717/peerj.4300 ◽

2018 ◽

Vol 6 ◽

pp. e4300 ◽

Cited By ~ 3

Author(s):

Ian Drake ◽

James F. White Jr ◽

Faith C. Belanger

Keyword(s):

Dna Sequences ◽

Sequence Data ◽

Elongation Factor ◽

Morphological Characteristics ◽

Fungal Endophyte ◽

Translation Elongation ◽

Ammophila Breviligulata ◽

Dna Sequence Data ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

The grass Ammophila breviligulata (American beachgrass) is known to host an endophyte of the genus Epichloë. Based on morphological characteristics it was originally identified as Acremonium typhinum var. ammophilae and is currently designated as Epichloë typhina var. ammophilae. However, the Epichloë species has not previously been identified based on DNA sequence data. Based on phylogenetic placement of beta-tubulin and translation elongation factor 1-alpha DNA sequences the endophyte is identified as a member of E. amarillans rather than E. typhina.

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New species and record of Zygophiala (Capnodiales, Mycosphaerellaceae) on apple from Montenegro

Phytotaxa ◽

10.11646/phytotaxa.195.3.2 ◽

2015 ◽

Vol 195 (3) ◽

pp. 227 ◽

Cited By ~ 2

Author(s):

Mian Zhang ◽

Liu Gao ◽

ShengPing Shang ◽

XiaoLu Han ◽

Rong Zhang ◽

...

Keyword(s):

Economic Value ◽

Elongation Factor ◽

Morphological Characteristics ◽

Apple Fruit ◽

Translation Elongation ◽

Sooty Blotch ◽

Elongation Factor 1 Alpha ◽

Multiple Sequences ◽

Elongation Factor 1 ◽

Partial Translation

Species of Zygophiala are components of the sooty blotch and flyspeck complex that blemish the cuticle of fruits reducing their economic value. In our study, six strains were isolated from apple fruit collected in orchards in Montenegro. Zygophiala montenegroensis is a newly described species based on its unique morphological characteristics and the analysis of multiple sequences including the internal transcribed spacer of the rDNA, the partial translation elongation factor 1-alpha and the actin genes. Zygophiala cylindrica is described as a new record in Montenegro and Europe.

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The holomorph of Arthrinium setariae sp. nov. (Apiosporaceae, Xylariales) from China

Phytotaxa ◽

10.11646/phytotaxa.483.2.7 ◽

2021 ◽

Vol 483 (2) ◽

pp. 149-159

Author(s):

NING JIANG ◽

CHENG-MING TIAN

Keyword(s):

Elongation Factor ◽

First Record ◽

Internal Transcribed Spacers ◽

Setaria Viridis ◽

Translation Elongation ◽

Host Genus ◽

Elongation Factor 1 Alpha ◽

Alpha Gene ◽

Elongation Factor 1 ◽

A New Species

Arthrinium is a widely distributed genus occurring on various hosts and substrates. Members of Arthrinium were distinguished based on the phylogeny of combined internal transcribed spacers (ITS), translation elongation factor 1-alpha gene (tef1), and beta-tubulin gene (tub2). In the present study, a specimen of obvious stromata on Setaria viridis was collected in Beijing Forestry University. Identification based on characters of sexual morph on Setaria viridis culms, asexual morph from manual media, and phylogeny indicated that it is different from any known species. Hence, we proposed a new species for this taxon named Arthrinium setariae. Arthrinium setariae has much larger conidia than its phylogenetically close species, A. jiangxiense, and represented the first record of Arthrinium inhabiting the host genus, Setaria.

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Morphology, Phylogeny and Pathogenicity of Pestalotioid Species on Camellia oleifera in China

Journal of Fungi ◽

10.3390/jof7121080 ◽

2021 ◽

Vol 7 (12) ◽

pp. 1080

Author(s):

Lingling Li ◽

Qin Yang ◽

He Li

Keyword(s):

Woody Plant ◽

Sequence Data ◽

Southern China ◽

Elongation Factor ◽

Morphological Characteristics ◽

Camellia Oleifera ◽

Translation Elongation Factor ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Elongation Factor 1

Tea-oil tree (Camellia oleifera) is an important edible oil woody plant with a planting area of over 3,800,000 hectares in southern China. Pestalotioid fungi are associated with a wide variety of plants worldwide along with endophytes, pathogens, and saprobes. In this study, symptomatic leaves of C. oleifera were collected from Guangdong, Guangxi, Hainan, Hunan, and Jiangsu Provinces and pestalotioid fungi are characterized based on combined sequence data analyses of internal transcribed spacer (ITS), beta tubulin (tub2), and translation elongation factor 1-alpha (tef-1α) coupled with morphological characteristics. As a result, seven species were confirmed, of which five species are described as new viz. N. camelliae-oleiferae, P. camelliae-oleiferae, P. hunanensis, P. nanjingensis, P. nanningensis, while the other two are reported as known species, viz., N. cubana and N. iberica. Pathogenicity assays showed that all species except for P. nanjingensis developed brown lesions on healthy leaves and P. camelliae-oleiferae showed stronger virulence.

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A new species and a new record of Thyronectria (Nectriaceae, Hypocreales) in China

Phytotaxa ◽

10.11646/phytotaxa.376.1.2 ◽

2018 ◽

Vol 376 (1) ◽

pp. 17

Author(s):

SHENG-NAN LI ◽

YING ZHAO ◽

CHENG-MING TIAN ◽

THEMIS J. MICHAILIDES ◽

RONG MA

Keyword(s):

New Species ◽

Elongation Factor ◽

First Record ◽

Translation Elongation ◽

Elongation Factor 1 Alpha ◽

Close Relatives ◽

Alpha Actin ◽

First Time ◽

Morphology Characteristics ◽

A New Species

A new species, Thyronectria berberidis, is described and illustrated based on collections on Berberis heteropoda from the Ili area of Xinjiang Uygur Autonomous Region in China. Thyronectria berberidis is characterized by superficial, gregarious ascomata that become cupulate upon drying, with (1–6)7-septate, ellipsoidal to fusiform ascospores. In addition, T. lamyi is reported for the first time in China. The morphology characteristics of T. berberidis and T. lamyi were compared to those of close relatives. Their phylogenetic positions were confirmed by analyses of combined sequences of alpha-actin, the internal transcribed spacer, the large nuclear ribosomal RNA subunit, translation elongation factor 1-alpha and beta-tubulin. This is the first record that Thyronectria is established in the B. heteropoda in China.

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