Molecular phylogenetics of Pterocyclus (Apiaceae) based on nrDNA ITS sequences: revised circumscription with a restored species

Phytotaxa ◽  
2021 ◽  
Vol 498 (2) ◽  
pp. 131-138
Author(s):  
JING ZHOU ◽  
JIN WEI ◽  
JUNMEI NIU ◽  
XIAOLI LIU ◽  
ZHENWEN LIU
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Phylogenetics ◽  
Identification Key ◽  
Its Sequences ◽  
Molecular Phylogenetic Analysis ◽  
Nrdna Its ◽  
Interspecific Relationships ◽  
Molecular Phylogenetic ◽  
Independent Species

The genus Pterocyclus Klotzsch, along with many others, constituted the taxonomically complex Pleurospermum s.l. To delimit its circumscription and clarify its interspecific relationships, molecular phylogenetic analysis and detailed specimen examination were carried out. Its status as an independent genus was confirmed, with four species recognized. Pterocyclus wolffianus, the synonymous species of Pterocyclus forrsetii should be restored as an independent species. A new taxonomic account for Pterocyclus and an identification key to its four species, are provided.

Molecular phylogenetic analysis of key Jatropha species inferred from nrDNA ITS and chloroplast (trnL-F and rbcL) sequences

2016 ◽  
Vol 38 (6) ◽  
pp. 557-566
Author(s):  
Guo-ye Guo ◽  
Sheng-hua Wang ◽  
Ying Xu ◽  
Lin Tang ◽  
Mao-qun Yu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Phylogenetic Analysis ◽  
Nrdna Its ◽  
Molecular Phylogenetic ◽  
Rbcl Sequences

A new species of Clavariadelphus (Basidiomycota: Clavariadelphaceae) from China based on morphological and molecular data

Phytotaxa ◽  
2020 ◽  
Vol 447 (1) ◽  
pp. 61-67
Author(s):  
LU XIA ◽  
LI FAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
North China ◽  
Molecular Data ◽  
Its Sequences ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
Morphological And Molecular Data ◽  
A New Species

Clavariadelphus griseoclavus sp. nov. is described and illustrated from North China. Morphologically, the new species is distinguished from other Clavariadelphus species by its grey to greyish basidiomata. Molecular phylogenetic analysis based on ITS sequences is provided and supports the establishment of the new species.

scholarly journals The cave beetle genus Anthroherpon is polyphyletic; molecular phylogenetics and description of Graciliella n. gen. (Leiodidae, Leptodirini)

2016 ◽  
Vol 85 (3) ◽  
pp. 337-359 ◽  
Author(s):  
Iva Njunjić ◽  
Michel Perreau ◽  
Kasper Hendriks ◽  
Menno Schilthuizen ◽  
Louis Deharveng
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Phylogenetics ◽  
Mitochondrial Gene ◽  
Morphological Characters ◽  
Molecular Phylogenetic Analysis ◽  
Phylogenetic Studies ◽  
Morphological Stasis ◽  
Molecular Phylogenetic ◽  
Morphological Differences ◽  
Cave Beetle

The subtribe Anthroherponina form an iconic group of obligate cave beetles, typical representatives of the Dinaric subterranean fauna, which is considered to be the richest in the world. Phylogenetic studies within this subtribe are scarce and based only on morphological characters, which, due to troglomorphic convergence, are frequently unreliable. Moreover, morphological stasis and morphological polymorphism make classification of taxa difficult. To test if characters that have traditionally been accepted as informative for Anthroherponina classification are indeed reliable, we evaluated the monophyly of the most speciesrich genus of this subtribe - Anthroherpon Reitter, 1889. Our study, based on a molecular phylogenetic analysis of fragments of the 18S, 28S, and COI (both 5’ and 3’ end) loci revealed that the genus Anthroherpon as conventionally defined is polyphyletic. To resolve this polyphyly, we defined one new additional genus, Graciliella n. gen., for which we then examined the intrageneric diversity using molecular and morphometric approaches. Molecular phylogenetic analysis of two COI mitochondrial gene fragments revealed the presence of four species inside Graciliella n. gen., including two new species, which we here describe as G. kosovaci n. sp. and G. ozimeci n. sp. To analyze interspecific morphological differences within Graciliella we performed a discriminant analysis based on 40 linear morphometric measurements. The results showed that differences between species and subspecies inside Graciliella, however subtle they may seem, are measurable and reproducible. All species of the genus are briefly diagnosed, an identification key is proposed and a distribution map of all taxa of Graciliella is provided.

Sedum nanlingense (Crassulaceae), a new species from Guangxi, China

Phytotaxa ◽  
2020 ◽  
Vol 447 (3) ◽  
pp. 176-184
Author(s):  
CHUN-YU ZOU ◽  
SHI-YONG MENG ◽  
ZHAO-CEN LU ◽  
YAN LIU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Internal Transcribed Spacer ◽  
Nuclear Dna ◽  
Morphological Characteristics ◽  
Its Sequences ◽  
Molecular Phylogenetic Analysis ◽  
Molecular Phylogenetic ◽  
A New Species

Sedum nanlingense (Crassulaceae), a new species from Guangxi, is described and illustrated here. We analyzed its morphological characteristics, which are largely similar to S. onychopetalum and S. kiangnanense. However, the morphology of this new species differs from other taxa in some important ways, including its 3-merous flowers, which contain six stamens and three carpels. Molecular phylogenetic analysis based on nuclear DNA internal transcribed spacer (ITS) sequences resolved S. nanlingense as a distinct clade, lending further support to its classification. Phylogenetic analysis showed that this new species is closely related to S. onychopetalum and S. kiangnanense.

scholarly journals The ITS region provides a reliable DNA barcode for identifying reishi/lingzhi (Ganoderma) from herbal supplements

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0236774
Author(s):  
Tess Gunnels ◽  
Matthew Creswell ◽  
Janis McFerrin ◽  
Justen B. Whittall
Keyword(s):  
Phylogenetic Analysis ◽  
Phylogenetic Analyses ◽  
Dna Barcode ◽  
Its Sequences ◽  
Molecular Phylogenetic Analysis ◽  
Herbal Supplement ◽  
Herbal Supplements ◽  
Expected Size Range ◽  
Molecular Phylogenetic ◽  
Species Authentication

The dietary supplement industry is rapidly growing yet, a recent study revealed that up to 60% of supplements may have substituted ingredients, some of which can be harmful contaminants or additives. When ingredients cannot be verified morphologically or biochemically, DNA barcoding complemented with a molecular phylogenetic analysis can be a powerful method for species authentication. We employed a molecular phylogenetic analysis for species authentication of the commonly used fungal supplement, reishi (Ganoderma lingzhi), by amplifying and sequencing the nuclear ribosomal internal transcribed spacer regions (ITS) with genus-specific primers. PCR of six powdered samples and one dried sample all sold as G. lucidum representing independent suppliers produced single, strong amplification products in the expected size-range for Ganoderma. Both best-hit BLAST and molecular phylogenetic analyses clearly identified the presence of G. lingzhi DNA in all seven herbal supplements. We detected variation in the ITS sequences among our samples, but all herbal supplement samples fall within a large clade of G. lingzhi ITS sequences. ITS-based phylogenetic analysis is a successful and cost-effective method for DNA-based species authentication that could be used in the herbal supplement industry for this and other fungal and plant species that are otherwise difficult to identify.

scholarly journals Molecular Phylogenetic Analysis of the AIG Family in Vertebrates

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1190
Author(s):  
Yuqi Huang ◽  
Minghao Sun ◽  
Lenan Zhuang ◽  
Jin He
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Family ◽  
Functional Characterization ◽  
Vertebrate Evolution ◽  
Molecular Phylogenetic Analysis ◽  
Whole Genome Duplications ◽  
Genome Duplications ◽  
Molecular Phylogenetic ◽  
Duplication Events ◽  
Inducible Genes

Androgen-inducible genes (AIGs), which can be regulated by androgen level, constitute a group of genes characterized by the presence of the AIG/FAR-17a domain in its protein sequence. Previous studies on AIGs demonstrated that one member of the gene family, AIG1, is involved in many biological processes in cancer cell lines and that ADTRP is associated with cardiovascular diseases. It has been shown that the numbers of AIG paralogs in humans, mice, and zebrafish are 2, 2, and 3, respectively, indicating possible gene duplication events during vertebrate evolution. Therefore, classifying subgroups of AIGs and identifying the homologs of each AIG member are important to characterize this novel gene family further. In this study, vertebrate AIGs were phylogenetically grouped into three major clades, ADTRP, AIG1, and AIG-L, with AIG-L also evident in an outgroup consisting of invertebrsate species. In this case, AIG-L, as the ancestral AIG, gave rise to ADTRP and AIG1 after two rounds of whole-genome duplications during vertebrate evolution. Then, the AIG family, which was exposed to purifying forces during evolution, lost or gained some of its members in some species. For example, in eutherians, Neognathae, and Percomorphaceae, AIG-L was lost; in contrast, Salmonidae and Cyprinidae acquired additional AIG copies. In conclusion, this study provides a comprehensive molecular phylogenetic analysis of vertebrate AIGs, which can be employed for future functional characterization of AIGs.

Molecular phylogenetic analysis of dinoflagellate Scrippsiella trochoidea isolated from the East Asian waters

2010 ◽  
Vol 28 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Xianghai Tang ◽  
Rencheng Yu ◽  
Qingchun Zhang ◽  
Yunfeng Wang ◽  
Tian Yan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
East Asian ◽  
Molecular Phylogenetic Analysis ◽  
Scrippsiella Trochoidea ◽  
Molecular Phylogenetic
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