scholarly journals The identity of Pentalonia nigronervosa Coquerel and P. caladii van der Goot (Hemiptera: Aphididae) based on molecular and morphometric analysis

Zootaxa ◽  
2010 ◽  
Vol 2358 (1) ◽  
pp. 25 ◽  
Author(s):  
R. G. FOOTTIT ◽  
H. E. L. MAW ◽  
K. S. PIKE ◽  
R. H. MILLER
Keyword(s):  
Dna Barcode ◽  
Elongation Factor ◽  
Nuclear Gene ◽  
Species Status ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Elongation Factor 1Α ◽  
Cytochrome Oxidase Subunit 1 ◽  
Pentalonia Nigronervosa ◽  
Mitochondrial Cytochrome Oxidase

Pentalonia nigronervosa (sensu Hardy 1931) samples from banana and from Zingiberaceae and Araceae species exhibit fixed differences in DNA sequence in mitochondrial cytochrome oxidase subunit 1 (“DNA barcode”) and in the nuclear gene elongation factor 1α, and have morphometric differences, including non-overlapping ranges in the length of the distal rostral segment. It is thus proposed that the name P. nigronervosa Coquerel be restricted to banana-feeding ‘nigronervosa’ specimens, and that the name Pentalonia caladii van der Goot be restored to full species status for specimens typically feeding on Zingiberaceae and Araceae.

Pamphilius japonicus feeds on Cornus: the first Old World record of Pamphiliidae (Hymenoptera) associated with Cornaceae

Zootaxa ◽  
2019 ◽  
Vol 4585 (2) ◽  
pp. 343
Author(s):  
AKIHIKO SHINOHARA ◽  
SHIN-ICHI IBUKI ◽  
TSUNEO KAKUDA ◽  
YUICHI KAMEDA
Keyword(s):  
Host Plant ◽  
First Record ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
World Record ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Old World ◽  
Cytochrome Oxidase Subunit 1 ◽  
Mitochondrial Cytochrome Oxidase

We identified newly discovered pamphiliid larvae feeding on Cornus in Tochigi and Ibaraki Prefectures, Honshu, Japan, with Pamphilius japonicus Shinohara, 1985, by molecular methods using mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences. The host plant of P. japonicus was unknown. This is the first record of the Cornaceae as a host plant of the Pamphiliidae in the Old World. The larvae were solitary leaf-rollers on Cornus and discovered mainly on small young trees in shadowy forests. 

A nomenclatural note on European Chalarus (Diptera: Pipunculidae): a new synonymy of C. elegantulus Jervis, 1992

Zootaxa ◽  
2010 ◽  
Vol 2656 (1) ◽  
pp. 67 ◽  
Author(s):  
CHRISTIAN KEHLMAIER
Keyword(s):  
Dna Barcode ◽  
Phylogenetic Study ◽  
Molecular Evidence ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Line Drawings ◽  
Taxonomic Decision ◽  
New Synonymy ◽  
Mitochondrial Cytochrome Oxidase ◽  
Control Trap

Recently, Kehlmaier & Assmann (2008) presented a revision of the European representatives of the big-headed fly genus Chalarus Walker, 1834. The work introduced four previously unknown taxa and provided diagnoses of all other known species, illustrated through numerous line drawings and photo-micrographs. In total, the authors treated 25 species including one nomen dubium. Three of these were known from males only and five solely from females. Taxonomic decision-making was backed up by molecular evidence, published partly therein and in the context of a phylogenetic study of the subfamily Chalarinae (Kehlmaier & Assmann 2010). Despite large collecting efforts, three taxa could not be analysed genetically, namely C. argenteus Coe, 1966, C. elegantulus Jervis, 1992 and C. proprius Jervis, 1992, all representing species based on females only.    Shortly after the publication of the above mentioned revision, a specimen of C. elegantulus was identified amongst material sent by Dr. Gunilla Ståhls (Finnish Museum of Natural History, Helsinki): 1&, Finland, Ab (Regio aboënsis), Karjalohja, Karkalinniemi, 66581:33221, control trap #2, 25.VI.–22.VII.2007, leg. G. Ståhls, coll. C. Kehlmaier. The 5’ half of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene was sequenced following standard lab procedures (see Kehlmaier & Assmann 2010) using the primer pair LCO1490 and HCO2198 (Folmer et al. 1994). The resulting DNA-barcode (Genbank sequence accession number: FN999909) was compared against a set of reference Chalarus barcode sequences and matched C. absconditus Kehlmaier in Kehlmaier & Assmann, 2008 syn. nov., a species know from male specimens only, sharing an identical haplotype.

scholarly journals Selecting Primers for RAPD, Microsatellite and Mitochondrial Cytochrome Oxidase Subunit 1 for Genetic Variation Analysis of Asian Corn Borer (Ostrinia furnacalis Guenée) Population in Java, Indonesia

2019 ◽  
Vol 23 (2) ◽  
pp. 234
Author(s):  
Ahmad Taufiq Arminudin ◽  
Y. Andi Trisyono ◽  
Arman Wijonarko ◽  
Suputa Suputa
Keyword(s):  
Genetic Diversity ◽  
Cytochrome Oxidase ◽  
Mitochondrial Cytochrome ◽  
Ostrinia Furnacalis ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Corn Borer ◽  
Asian Corn Borer ◽  
Cytochrome Oxidase Subunit 1 ◽  
Mitochondrial Cytochrome Oxidase

Primer plays an important role in studying genetic diversity of an insect species. This research was aimed to select the suitable primers to visualize the genetic diversity of Asian corn borer (Ostrinia furnacalis) using Random Amplified Polymorphic DNA (RAPD), microsatellite, and mitochondrial cytochrome oxidase subunit 1 gene (mtCO1). Twenty four RAPD primers (OPA1, OPA4, OPA7,OPA8, OPA10, OPA11, OPA12, OPA13, OPB7, OPB10, OPB11, OPB12, OPB15, OPC4, OPC5, OPC14, OPC16, OPC18, OPC20, OPD3, OPD8, OPD10, OPD13, OPD14) and five microsatellite primers (T3, T4, T5, T81, D25) resulted high polymorphic informations of the genetics of O. furnacalis in Java Indonesia. Universal primers, Lep and Heb were appropriateto do molecular identification of O. furnacalis based on BLAST system on GenBank and BOLD systems.

scholarly journals DNA Barcoding for Scorpion Species from New Valley Governorate in Egypt Reveals Different Degrees of Cryptic Speciation and Species Misnaming

Conservation ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 228-240
Author(s):  
Khaled Mohammed-Geba ◽  
Ahmad Hamed Obuid-Allah ◽  
Naser Abdellatif El-Shimy ◽  
Mohamed Abd El-Moez Mahbob ◽  
Rouwaida Saadawy Ali ◽  
...  
Keyword(s):  
Significant Proportion ◽  
Pcr Amplification ◽  
Coi Gene ◽  
Cryptic Speciation ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Major Interest ◽  
Cytochrome Oxidase Subunit 1 ◽  
High Level ◽  
Mitochondrial Cytochrome Oxidase

(1) Background: Scorpions (Arthropoda: Arachnida) represent a diverse group of invertebrates, accounting for a significant proportion of earth’s predators and ecosystems’ modulators. Surviving mostly in hardly reachable nests, and representing key hazards to human health, they attracted major interest for characterizing their eco-, morpho-, and genotypes. (2) Methods: Four scorpion species were collected from the New Valley governorate in Upper Egypt, where a high level of scorpionism and related neurological symptoms are found, that were Leiurus quinquestriatus, Androctonus amoreuxi, Orthochirus innesi, Buthacus leptochelys. They were DNA barcoded, genetically and phylogenetically analyzed through PCR amplification and sequencing of the mitochondrial cytochrome oxidase subunit 1 (COI) gene hypervariable 5′ region. (3) Results: New, morphologically authenticated scorpion barcodes could be added to the barcoding databases. However, several discrepancies and barcode database inadequacies could be revealed. Moreover, taxon-specific patterns for nitrogenous bases’ distribution could be identified, resulting in a significantly high percentage of COI barcode guanine in scorpionids, in comparison to araneids and opilions. (4) Conclusions: For a group of animals where both cryptic speciation and a high risk of human envenomation are evident, the findings of the current study strongly recommend continuous and comprehensive research efforts dealing with morphogenetic authentication for different species of scorpions.

DNA Barcodes Identify the Larvae and Unassociated Male of Three Onycholyda Sawflies (Hymenoptera, Pamphiliidae) from China

Zootaxa ◽  
2018 ◽  
Vol 4403 (1) ◽  
pp. 123
Author(s):  
AKIHIKO SHINOHARA ◽  
TSUNEO KAKUDA ◽  
MEICAI WEI ◽  
YUICHI KAMEDA
Keyword(s):  
Host Plant ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit 1 ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase ◽  
Unknown Male

A molecular analysis based on mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences has indicated that larvae collected in Sichuan and Zhejiang Provinces, China, belong to Onycholyda xanthogaster Shinohara, 1999, and O. fulvicornis Shinohara, in Shinohara & Wei, 2016 (Hymenoptera, Pamphiliidae), and that a male Onycholyda specimen from Mt. Tianmushan, Zhejiang Province is the hitherto unknown male of O. tianmushana Shinohara & Xiao, 2006. The first host plant records are Rubus inopertus (Focke) Focke (Rosaceae) for O. xanthogaster and Rubus hirsutus Thunb. for O. fulvicornis. The larvae of O. xanthogaster and O. fulvicornis are briefly described and O. xanthogaster is newly recorded from Sichuan Province. The male of O. tianmushana is described for the first time. 

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

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