scholarly journals Selecting Primers for RAPD, Microsatellite and Mitochondrial Cytochrome Oxidase Subunit 1 for Genetic Variation Analysis of Asian Corn Borer (Ostrinia furnacalis Guenée) Population in Java, Indonesia

2019 ◽  
Vol 23 (2) ◽  
pp. 234
Author(s):  
Ahmad Taufiq Arminudin ◽  
Y. Andi Trisyono ◽  
Arman Wijonarko ◽  
Suputa Suputa
Keyword(s):  
Genetic Diversity ◽  
Cytochrome Oxidase ◽  
Mitochondrial Cytochrome ◽  
Ostrinia Furnacalis ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Corn Borer ◽  
Asian Corn Borer ◽  
Cytochrome Oxidase Subunit 1 ◽  
Mitochondrial Cytochrome Oxidase

Primer plays an important role in studying genetic diversity of an insect species. This research was aimed to select the suitable primers to visualize the genetic diversity of Asian corn borer (Ostrinia furnacalis) using Random Amplified Polymorphic DNA (RAPD), microsatellite, and mitochondrial cytochrome oxidase subunit 1 gene (mtCO1). Twenty four RAPD primers (OPA1, OPA4, OPA7,OPA8, OPA10, OPA11, OPA12, OPA13, OPB7, OPB10, OPB11, OPB12, OPB15, OPC4, OPC5, OPC14, OPC16, OPC18, OPC20, OPD3, OPD8, OPD10, OPD13, OPD14) and five microsatellite primers (T3, T4, T5, T81, D25) resulted high polymorphic informations of the genetics of O. furnacalis in Java Indonesia. Universal primers, Lep and Heb were appropriateto do molecular identification of O. furnacalis based on BLAST system on GenBank and BOLD systems.

scholarly journals Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Vista Budiariati ◽  
Trini Susmiati ◽  
Siti Munawaroh ◽  
Rachmawati Cahyaningtyas Arie Putri ◽  
Rini Widayanti Widayanti
Keyword(s):  
Genetic Diversity ◽  
Amino Acid ◽  
Cytochrome Oxidase ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Oxidase Subunit ◽  
Coii Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
Cytochrome Oxidase Subunit Ii

Abstract. Budiariati V, Susmiati T, Waroh S, Putri RCA, Widayanti R. 2021. Genetic diversity of indigenous catfish from Indonesia based on mitochondrial Cytochrome Oxidase Subunit II gene. Biodiversitas 22: 593-600. Catfish is one of the most demanding fish in Indonesia and served in a variety of traditional culinary. Due to their identical morphology and close relation between species in the order of Siluriformes, it is quite tricky to distinguish the species. This can be a threat to develop catfish production in Indonesia since there is a wide variety of catfish species in this mega biodiversity country. The study aimed to analyze the genetic diversity of Indonesian indigenous catfish especially those known as Baung fish by local people based on COII gene. The study also aimed to determine the phylogenetic relationship between the samples and compare them with the GenBank data. A total of 24 samples used in this study from 8 different rivers from 3 different islands and two samples were collected from coastal areas. The study results showed that there is genetic diversity of the Indonesian indigenous catfish based on COII gene. The sequences among 24 samples showed that from 691 nucleotides of COII gene, there were very subtle nucleotides differences of samples that originated from Bojonegoro, Magelang, and samples collected from Baru Beach, Yogyakarta. Based on COII amino acid sequences, 6 polymorphic amino acid sites were on-site number 64, 115, 123, 129, 144, and 165. The samples encoded LLB1 and LPB1 from Baru Beach, Yogyakarta, showed highest different amino acids in the six sites. Samples from the river of Central Java, Sumatra, and Kalimantan belonged to Bagridae family and consist of two different species Hemibagrus sp. and Mystus sp while samples from East Java belonged to Pangasiidae family. The Samples from coastal belonged to Ariidae family.

First rearing record and DNA sequence of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) associated with Saxifraga paniculata Miller from the Czech Republic

2020 ◽  
Vol 69 (3) ◽  
pp. 283-288
Author(s):  
Miloš Černý ◽  
Jiří Kocián ◽  
Jan Ševčík
Keyword(s):  
Czech Republic ◽  
Cytochrome Oxidase ◽  
Dna Sequence ◽  
Partial Sequence ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
The Czech Republic ◽  
Mitochondrial Cytochrome Oxidase

Abstract A male of Chromatomyia aizoon (Hering, 1932) (Diptera: Agromyzidae) was reared from a leaf mine at Saxifraga paniculata collected in Štramberk (Northern Moravia, Czech Republic). Partial sequence of the mitochondrial cytochrome oxidase subunit I (COI barcode region, 658 bp) is also provided.

Redefinition of Cornexcisia Fan & Kano, 2000 (Diptera: Sarcophagidae), with the first description of the female of Sarcophaga kurahashii (Shinonaga & Tumrasvin, 1979)

Zootaxa ◽  
2019 ◽  
Vol 4668 (3) ◽  
pp. 410-420
Author(s):  
CHAO WANG ◽  
YUNYUN GAO ◽  
THOMAS PAPE ◽  
DONG ZHANG
Keyword(s):  
Cytochrome Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Gene Sequences ◽  
Cytochrome Oxidase Subunit ◽  
Cytochrome Oxidase Subunit I ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Senior Synonym ◽  
First Time ◽  
Mitochondrial Cytochrome Oxidase

Sarcophaga Meigen, 1826 is proposed as a senior synonym of Cornexcisia Fan & Kano, 2000, syn. nov. and Fanzideia Xue, Verves & Du, 2011, syn. nov. Cornexcisia Fan & Kano, 2000, stat. rev. is given status as a subgenus and is considered a senior synonym of Fanzideia Xue, Verves & Du, 2011, syn. nov. at the subgeneric level. Cornexcisia is argued to contain S. (Cornexcisia) longicornuta (Fan & Kano, 2000), comb. nov., S. (C.) cygnocerca (Xue, Verves & Du, 2011), comb. nov., S. (C.) kurahashii (Shinonaga & Tumrasvin, 1979), subgen. comb. nov. (from Phallosphaera Rohdendorf) and S. (C.) suthep Pape & Bänziger, 2003, subgen. comb. nov. (from Rosellea Rohdendorf). Sarcophaga (C.) kurahashii is newly recorded from China (Yunnan), the male is redescribed and the female is described for the first time, supported by photographs, illustrations and mitochondrial cytochrome oxidase subunit I (COI) gene sequences. Species of Cornexcisia share an exceptionally long postpedicel in the female and the following apomorphic distiphallic appendages in the male: juxta ventro-proximally with an apically divided arm with cuticular pile, and lateral styli bifurcated from the base with each branch elongate, gently curved and slightly expanded apically. A key to the species of Cornexcisia is provided. 

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