Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis

Zootaxa ◽  
2010 ◽  
Vol 2383 (1) ◽  
pp. 1 ◽  
Author(s):  
FRANK GLAW ◽  
JÖRN KÖHLER ◽  
IGNACIO DE LA RIVA ◽  
DAVID R. VIEITES ◽  
MIGUEL VENCES
Keyword(s):  
New Species ◽  
Body Size ◽  
National Park ◽  
Integrative Approach ◽  
Morphological Evidence ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Advertisement Calls ◽  
Ranomafana National Park ◽  
Threat Status

We describe ten new species of treefrogs assigned to the genus Boophis (Anura: Mantellidae) and resurrect two species from synonymy, based on materials collected during fieldwork in Madagascar, carried out mainly between 2000 and 2007. Our comparative database assembled over the past years comprises fresh material for molecular analysis from all 58 nominal Boophis species, and advertisement call recordings from all except three species. We follow an integrative approach and combine molecular, bioacoustic and morphological evidence to diagnose the new species. In most cases, the new species have uncorrected molecular divergences of over 4–5% in the mitochondrial 16S rRNA gene to their closest relatives. In some cases the divergences are lower (2–2.5%) but are then accompanied by distinct differences in advertisement calls or morphology. Boophis piperatus sp. nov. from Ranomafana National Park is a small brown species assigned to the B. majori group that is similar to B. miniatus but differs in morphology and advertisement calls. Boophis arcanus sp. nov. is assigned to the B. majori group as well and is known from only two female specimens from a site close to Ranomafana; it is described mainly based on its strong genetic differentiation (> 7.2% to all other species). Boophis entingae sp. nov. is a species of the Boophis goudoti group occurring in northern Madagascar, similar to and sympatric with B. brachychir, but with a strongly different advertisement call. Boophis roseipalmatus sp. nov. belongs to the B. goudoti group, is similar to B. madagascariensis, and appears to replace this species in most of northern Madagascar, with possible areas of sympatry in the north east. Boophis spinophis sp. nov. is an enigmatic, morphologically highly divergent species from Ranomafana National Park that belongs into the B. goudoti group but differs from all other spe-4 · Zootaxa 2383 © 2010 Magnolia Presscies in the group by having distinct dermal tubercles along the lateral parts of the shank and around the elbow. Boophis praedictus sp. nov. is a sibling species of B. albilabris in the B. albilabris group, diagnosable by its red iris periphery and distributed in rainforest along the east coast. Boophis sandrae sp. nov. belongs to the B. luteus group and is superficially similar to the sympatric B. elenae, but has a faster call and smaller body size. Boophis miadana sp. nov. and B. haingana sp. nov., both in the B. albipunctatus group and syntopically occurring at Andohahela National Park, are related to B. ankaratra and B. schuboeae and differ mainly by their advertisement calls. Boophis luciae sp. nov., also in the B. albipunctatus group, differs from the sympatric B. albipunctatus and B. sibilans by having slightly smaller body size and different advertisement calls. We furthermore resurrect Rhacophorus obscurus Boettger, 1913 (as Boophis obscurus in the B. goudoti group) from the synonymy of Boophis goudoti as well as Rhacophorus andrangoloaka Ahl, 1928 (as Boophis andrangoloaka in the B. microtympanum group) from the synonymy of Boophis rhodoscelis, and propose to consider Rhacophorus brevirostris Ahl, 1928 as junior synonym of Boophis andrangoloaka. We discuss our integrative methodological approach and the different lines of evidence used to delimitate the species described or resurrected herein. By applying IUCN Redlist criteria, we evaluate the threat status of the species considered: six species are classified Data Deficient (B. arcanus, B. haingana, B. miadana, B. piperatus, B. praedictus, B. spinophis), four Vulnerable (B. andrangoloaka, B. entingae, B. roseipalmatus, B. sandrae), and two Least Concern (B. luciae, B. obscurus).

A new species of Mantidactylus (subgenus Chonomantis) from Ranomafana National Park, eastern Madagascar (Amphibia, Anura, Mantellidae)

Zootaxa ◽  
2011 ◽  
Vol 2772 (1) ◽  
pp. 52
Author(s):  
PARFAIT BORA ◽  
OLGA RAMILIJAONA ◽  
NOROMALALA RAMINOSOA ◽  
MIGUEL VENCES
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
National Park ◽  
Rrna Gene ◽  
Ranomafana National Park ◽  
Threat Status ◽  
East Region ◽  
Southern Central ◽  
Mitochondrial 16S Rrna Gene ◽  
Data Deficient

We describe a new frog species of Mantidactylus belonging to the subgenus Chonomantis from Ranomafana National Park, in the Southern Central East region of Madagascar, at mid-elevations (about 950 m above sea level). Specimens of Mantidactylus paidroa sp. nov. were observed during the day in cavities under large rocks next to a stream in rainforest. Their advertisement calls are unique in Chonomantis and consist of a long series of note pairs. The new species shows a high divergence to other Chonomantis species in DNA sequences of the mitochondrial 16S rRNA gene (5.6–10.8%). Mantidactylus paidroa is so far only known from several streams in Ranomafana National Park but may have been overlooked at other rainforest sites in eastern Madagascar. We propose an IUCN threat status of Data Deficient for this new species.

scholarly journals A new species of spotted leaf frog, genusPhasmahyla(Amphibia, Phyllomedusidae) from Southeast Brazil

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4900 ◽  
Author(s):  
Elvis Almeida Pereira ◽  
Lucas Custódio Lomba Rocha ◽  
Henrique Folly ◽  
Hélio Ricardo da Silva ◽  
Diego José Santana
Keyword(s):  
New Species ◽  
Anterior Part ◽  
Distinct Species ◽  
Type Locality ◽  
Integrative Approach ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Phylogenetic Distance ◽  
Southeast Brazil ◽  
A New Species

Based on concordant differences in male advertisement call, tadpole morphology, and absence of haplotype sharing in the barcoding 16S mitochondrial DNA, we describe here a new species of spotted leaf frog of the genusPhasmahylafrom Atlantic Forest, State of Rio de Janeiro, Southeast Brazil. The new species is most similar toP. cochranae(type locality) andP. spectabilis(type locality). It differs from these species by the size of the calcar, moderate-sized body (snout-vent length 30.4–34.4 mm in adult eight males), and in the advertisement call. The tadpoles ofPhasmahyla lisbellasp. nov. differ fromP. exilis,P. spectabilis,P. timbo,P. guttataandP. jandaiabecause they do not have row of teeth in the anterior part; differ fromP. cruziby the shape of the anterior end of the oral disc. Through genetic data (phylogenetic distance and haplotype genealogy) we diagnosed the new species where the genetic divergences among its congeners is about 3–6% in a fragment of the 16S rRNA gene, which is above the threshold typically characterizing distinct species of anurans. However, the new species can be distinguished from other congeneric species based on an integrative approach (molecular, bioacoustics, larval, and adult morphology).

scholarly journals A new species of Proceratophrys Miranda-Ribeiro, 1920 (Anura, Odontophrynidae) from Southern Amazonia, Brazil

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12012
Author(s):  
Diego J. Santana ◽  
Leandro Alves da Silva ◽  
Anathielle Caroline Sant’Anna ◽  
Donald B. Shepard ◽  
Sarah Mângia
Keyword(s):  
New Species ◽  
Distinct Species ◽  
Integrative Approach ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Hydroelectric Dams ◽  
Mato Grosso ◽  
Southern Amazonia ◽  
A New Species ◽  
The 16S Rrna Gene

Based on concordant differences in morphology, male advertisement call, and 16S mtDNA barcode distance, we describe a new species of Proceratophrys from southern Amazonia, in the states of Mato Grosso and Pará, Brazil. The new species is most similar to P. concavitympanum and P. ararype but differs from these species by its proportionally larger eyes and features of the advertisement call. Additionally, genetic distance between the new species and its congeners is 3.0–10.4% based on a fragment of the 16S rRNA gene, which is greater than the threshold typically characterizing distinct species of anurans. Using an integrative approach (molecular, bioacoustics, and adult morphology), we were able to distinguish the new species from other congeneric species. The new species is known only from the type locality where it is threatened by illegal logging and gold mining as well as hydroelectric dams.

Systematics of the Australo-Papuan tree frogs known as Litoria bicolor (Anura:Hylidae) in the Papuan region

2008 ◽  
Vol 56 (4) ◽  
pp. 257 ◽  
Author(s):  
J. I. Menzies ◽  
S. J. Richards ◽  
M. J. Tyler
Keyword(s):  
New Species ◽  
Body Size ◽  
Distinct Species ◽  
Northern Territory ◽  
Advertisement Call ◽  
Body Proportions ◽  
Advertisement Calls ◽  
Origin And Evolution ◽  
Call Structure ◽  
Tree Frogs

We examined differences in morphology and advertisement calls of a large sample of frogs from the Australo-Papuan Region that resemble Litoria bicolor, and compared them with examples of that species from Australia. Consistent differences in body size, body proportions, and advertisement call structure among populations demonstrate that at least seven distinct species occur in the Australo-Papuan region, and that only the population represented by the holotype from the Northern Territory of Australia is Litoria bicolor s.s. Herein we describe four new species from the Papuan Region and comment on the origin and evolution of the Papuan members of the Litoria bicolor complex in the region.

An African glass frog: A new Hyperolius species (Anura: Hyperoliidae) from Nyungwe National Park, southern Rwanda

Zootaxa ◽  
2012 ◽  
Vol 3391 (1) ◽  
pp. 52 ◽  
Author(s):  
J. MAXIMILIAN DEHLING
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
National Park ◽  
Morphological Characters ◽  
Medium Size ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Adult Males ◽  
Mitochondrial 16S Rrna Gene

A new species of Hyperolius is described from Nyungwe National Park in southern Rwanda. The new species differs fromits congeners by a unique combination of morphological characters, including a light green dorsum and a transparentventral skin resembling glass frogs of the Neotropical family Centrolenidae, blue-coloured bones, reduced toe webbing,relatively wide head, acuminate snout, small to medium size (SUL of adult males 18.8–23.2 mm), and the presence ofnuptial pads. The advertisement call differs from all calls of other species of the genus that have been analyzed.Comparison of the mitochondrial 16S rRNA gene showed a relatively close relationship to H. castaneus, H.cystocandicans, H. discodactylus, H. frontalis, and H. lateralis. The 16S sequence of the new species differs by at least 4.5% in the uncorrected p-distance from all available sequences of other species of the genus.Ubwoko bushya bwa Hyperolius buboneka muri parike nasiynali ya Nyungwe mu majyepfo yu Rwanda. Ubu bwokobushya butandukaniye na bugenzi bwabwo gusa kurusobe rw’miterere yabwo, harimo dorsum ifite ibara ry’cyatsi cyeru-rutse n’ruhu rubonerana rwo kunda, amagufwa afite ibara ry’bururu, n’tunodufatanijwe n’gahu, umutwe wenda kubamunini, umunwa uurungushuye, umubyimba uri hagati ya 18,8 mm kugera kuri 23,3 mm ku ngabo nkuru, ikagira n’mag-aragamba ku ruhu. Kuzitangaza kwazo byagiye bitandukana n’matangazo yabaye kubundi bwoko bwazo. Ugereanijen’miterere yazo idahinduka (mitochondrial 16S rRNA gene) niyizindi usanga bifitanye isano ya bugufi na H. castaneus,H.cystocandicans, H.discodactylus, H.frontalis na H. lateralis. Urukurikirane rwa 16S y’bu bwoko bushya rutandukanye kuri 4,5% ugereranije n’nkurikirana zabaye z’bundi bwoko busa n’bu.

Description of a new Malagasy treefrog (Boophis) occurring syntopically with its sister species, and a plea for studies on non-allopatric speciation in tropical amphibians

2012 ◽  
Vol 33 (3-4) ◽  
pp. 503-520 ◽  
Author(s):  
Miguel Vences ◽  
Marcelo Gehara ◽  
Jörn Köhler ◽  
Frank Glaw
Keyword(s):  
National Park ◽  
Nuclear Dna ◽  
Distinct Species ◽  
Sister Species ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Ranomafana National Park ◽  
Southern Central ◽  
A New Species ◽  
The 16S Rrna Gene

Based on concordant differences in male advertisement call, tadpole morphology, and absence of haplotype sharing in mitochondrial and nuclear DNA markers, we describe a new species of treefrog from Ranomafana National Park in the southern central east of Madagascar. In its adult stage Boophis narinsi sp. n. is highly similar to its sister species, Boophis majori, but appears to differ in having longer hindlimbs. The genetic divergences between these two species (2.5-3.3% in a fragment of the 16S rRNA gene, depending on fragment length and individual haplotype analyzed) are below the threshold typically characterizing distinct species of anurans. Together with their relatively small and largely overlapping ranges and their sympatric occurrence in Ranomafana National Park, this indicates that they potentially could have originated rather recently by adaptive speciation under parapatric or sympatric conditions. Most studies on amphibian speciation have so far by default assumed vicariant speciation. We suggest that alternative speciation scenarios should be considered in future works and characterize settings in which more reliable assessments of adaptive parapatric or sympatric speciation could be carried out.

scholarly journals A new yellow-toed Platypelis species (Anura, Microhylidae, Cophylinae) from the Maroantsetra region, northeastern Madagascar

2019 ◽  
Vol 3 (1) ◽  
pp. 75-83
Author(s):  
Mark D. Scherz ◽  
Jörn Köhler ◽  
Miguel Vences ◽  
Frank Glaw
Keyword(s):  
New Species ◽  
National Park ◽  
Small Body ◽  
Genetic Distances ◽  
Iucn Red List ◽  
Advertisement Call ◽  
Rrna Gene ◽  
External Appearance ◽  
Mitochondrial 16S Rrna Gene ◽  
Iucn Red List Criteria

We describe a new species of arboreal narrow-mouthed frog, genus Platypelis, from Ambodivoangy near Maroantsetra in northeastern Madagascar. The new species, Platypelisandosp. nov., is characterised by small body size (under 19 mm), a generally rather slender body, yellowish finger and toe tips, and a dark brown dorsal chevron. Its advertisement call is a single, moderately long, high-pitched whistle repeated at regular intervals. It is the sister species of P.ravus from Marojejy National Park, but differs from that species by considerable pairwise genetic distances (7.9%) in a fragment of the mitochondrial 16S rRNA gene, and also in bioacoustic and morphological features, especially the absence of yellow on the posterior abdomen. It is also surprisingly similar in external appearance to Cophylaoccultans and C.maharipeo, to which it is not, however, closely related; these species are most easily discerned based on their calls. Platypelisandosp. nov. joins the ranks of several species recently described from Ambodivoangy with close affiliations to species in the nearby Marojejy National Park, that are still divergent at species level. The species qualifies as Critically Endangered according to the IUCN Red List criteria, in line with other species recently assessed from this area, but we urge that more research be conducted in the nearby forests to extend the range of this and other species known only from Ambodivoangy.

Yet another small brown frog from high altitude on the Marojejy Massif, northeastern Madagascar (Anura: Mantellidae)

Zootaxa ◽  
2017 ◽  
Vol 4347 (3) ◽  
pp. 572 ◽  
Author(s):  
MARK D. SCHERZ ◽  
JARY H. RAZAFINDRAIBE ◽  
ANDOLALAO RAKOTOARISON ◽  
NADI M. DIXIT ◽  
MOLLY C. BLETZ ◽  
...  
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
High Altitude ◽  
National Park ◽  
Pairwise Distance ◽  
Advertisement Call ◽  
Rrna Gene ◽  
Mitochondrial 16S Rrna Gene ◽  
High Diversity

Madagascar hosts a high diversity of small brown frogs. In this paper, we add another one by describing Gephyromantis (Duboimantis) tohatra sp. nov. The new species is a small brown mantellid frog discovered on a recent expedition to Marojejy National Park in northeastern Madagascar. It is characterised, among other things, by its small size (snout-vent length ~33 mm), an orange to yellowish belly, two dorsolateral ridges, and a distinctive call composed of 7–10 pulsed notes. The new species occurs sympatrically with other members of the subgenus Duboimantis at high altitude (~1700 m above sea level), including its sister species G. schilfi from which it radically differs by advertisement call and by a substantial genetic divergence of 4.3% uncorrected pairwise distance in the mitochondrial 16S rRNA gene. It thus joins the diverse assemblage of Gephyromantis species known from high altitudes on the mountain massifs of northern Madagascar. 

Description of a new species of Auriculostoma (Digenea: Allocreadiidae) from Characidium heirmostigmata (Characiformes: Crenuchidae) from Argentina, using morphological and molecular data

2021 ◽  
Vol 95 ◽  
Author(s):  
M.M. Montes ◽  
J. Barneche ◽  
Y. Croci ◽  
D. Balcazar ◽  
A. Almirón ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
National Park ◽  
Molecular Data ◽  
Rrna Gene ◽  
28S Rrna ◽  
The Family ◽  
Parasitological Survey ◽  
Morphological And Molecular Data ◽  
A New Species

Abstract During a parasitological survey of fishes at Iguazu National Park, Argentina, specimens belonging to the allocreadiid genus Auriculostoma were collected from the intestine of Characidium heirmostigmata. The erection of the new species is based on a unique combination of morphological traits as well as on phylogenetic analysis. Auriculostoma guacurarii n. sp. resembles four congeneric species – Auriculostoma diagonale, Auriculostoma platense, Auriculostoma tica and Auriculostoma totonacapanensis – in having smooth and oblique testes, but can be distinguished by a combination of several morphological features, hosts association and geographic distribution. Morphologically, the new species can be distinguished from both A. diagonale and A. platense by the egg size (bigger in the first and smaller in the last); from A. tica by a shorter body length, the genital pore position and the extension of the caeca; and from A. totonacapanensis by the size of the oral and ventral sucker and the post-testicular space. Additionally, one specimen of Auriculostoma cf. stenopteri from the characid Charax stenopterus (Characiformes) from La Plata River, Argentina, was sampled and the partial 28S rRNA gene was sequenced. The phylogenetic analysis revealed that A. guacurarii n. sp. clustered with A. tica and these two as sister taxa to A. cf. stenopteri. The new species described herein is the tenth species in the genus and the first one parasitizing a member of the family Crenuchidae.

scholarly journals New and interesting species of the genera Galumna and Pergalumna (Acari, Oribatida, Galumnidae) from the Montagne d'Ambre National Park, Madagascar

Acarologia ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 64-74
Author(s):  
Sergey G. Ermilov ◽  
Josef Starý
Keyword(s):  
New Species ◽  
Body Size ◽  
National Park ◽  
Similar Species ◽  
Two New Species ◽  
Ethiopian Region ◽  
First Time ◽  
Interesting Species ◽  
Median Pore

This work includes taxonomic and faunistic data on galumnid mites (Oribatida, Galumnidae) belonging to the genera Galumna and Pergalumna collected from the Montagne d'Ambre National Park, North Madagascar. Two new species are described: Galumna sandormahunkai n. sp. differs from its closest species, Galumna sphagni by the larger body size, the presence of strongly protruding rostrum, lanceolate, pointed apically bothridial setae, the direction of lamellar lines, and the absence of median pore; Pergalumna janosbaloghi n. sp. differs from the most similar species, Pergalumna aegra, by the smaller body size and the presence of long lamellar setae and elongate, distinctly or slightly triangular porose areas Aa. Galumna granalata and Pergalumna amamiensis are recorded in the Ethiopian region for the first time; Pergalumna conspicua and P. frater are recorded in Madagascar for the first time.

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