Morphological description and DNA barcoding of Hydrobaenus laticaudus Sæther, 1976 (Diptera: Chironomidae: Orthocladiinae) from Amur River basin (Russian Far East)

Zootaxa ◽  
2019 ◽  
Vol 4674 (2) ◽  
pp. 225-234
Author(s):  
EUGENYI A.  MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO

Illustrated redescription of adult male and description for the first time of pupa and fourth instar larva, as well as DNA barcoding, of Hydrobaenus laticaudus Sæther in comparison with close related known species of Hydrobaenus Fries, and an updated part of a key to species of Hydrobaenus from the Russian Far East are provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. For the estimation of interspecific distances and constructing detailed Bayesian tree within Hydrobaenus, we have obtained DNA barcoding for the two other species, H. distinctus and Hydrobaenus sp.1. The intraspecific sequence divergence of H. laticaudus based on the Kimura-2-parameter (K2P) distance ranged from 0.0–0.031, whereas the interspecific sequence divergence based on the K2P distance ranged from 0.106-0.197% between H. laticaudus and other species of genus Hydrobaenus. The well-supported monophyly as well as results of an ABGD analysis confirms the validity of H. laticaudus. 

Zootaxa ◽  
2006 ◽  
Vol 1151 (1) ◽  
pp. 47
Author(s):  
OKSA V. ZORINA

Two new species of Beckidia Sæther, B. biraensis and B. connexa, from the Amur River Basin are described and figured as males. The male of B. tethys (Townes) is redescribed and figured and the species is recorded for the first time from Russia. The generic diagnosis is emended and a key to the males of Beckidia from the Holarctic Region is given.


2021 ◽  
Vol 45 ◽  
pp. 62-71
Author(s):  
Evgeny S. Koshkin ◽  
Vitaly G. Bezborodov ◽  
Aleksandr A. Kuzmin

New finds of seven East Asian Lepidoptera species in the Russian part of the southern Priamurye (Amur Basin area) are presented. Lobocla bifasciata (Bremer et Grey, 1853) (Hesperiidae), Acosmeryx naga (Moore, [1858]) and Rhagastis mongoliana (Butler, [1876]) (Sphingidae) were found in the Amur Oblast’ for the first time. Ambulyx tobii (Inoue, 1976) (Sphingidae) was first discovered in the Khabarovsk Kray; it was also found in the Chernigovsky district of the Primorsky Kray, northward from the previously known localities. New finds of very rare nemoral species, Chrysozephyrus brillantinus (Staudinger, 1887) (Lycaenidae) and Clanis undulosa Moore, 1879 (Sphingidae), in the Khabarovsk Kray are reported. It is shown that the subtropical and tropical species Siglophora sanguinolenta (Moore, 1888) (Nolidae), first collected in Russia in 2020, has successfully naturalized in the southern Khabarovsk Kray. New finds of these species indicate that the species have extended their ranges northward and naturalized in new areas. This has occurred due to climate changes in the Amur River basin over the past decades such as the rising average annual and average air temperatures during cold periods.


Zootaxa ◽  
2017 ◽  
Vol 4247 (3) ◽  
pp. 313 ◽  
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO

Chironomids of the subgenus Chaetocladius s. str. from the Amur River basin are revised using both morphological characters and molecular data. Three new species, C. egorych sp. nov., C. lopatinskiy sp. nov. and C. yavorskayae sp. nov., are described and figured. The pupa of C. fedotkin is described for the first time. Adult males of C. ligni and C. piger, little-known in the Far East, are redescribed and annotated, and key to males of the Chaetocladius s. str. from the Amur River basin is provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. Comparisons with corresponding regions of COI between 5 species in the subgenus produced K2P genetic distances of 8.3–12.6%, values well associated with interspecific variation. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius s. str. 


Zootaxa ◽  
2017 ◽  
Vol 4286 (2) ◽  
pp. 277
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
OLEG A. VELIAEV

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank. 


2017 ◽  
Vol 48 ◽  
pp. 13-22
Author(s):  
N. S. Probatova

Calamagrostis are described from the Russian Far East. Chromosome numbers are reported for two new taxa. Calamagrostis burejensis Prob. et Barkalov, 2n = 28 (sect. Calamagrostis), C. zejensis Prob., 2n = 28 (sect. Deyeuxia), and C. × amgunensis Prob. (C. amurensis Prob. × C. neglecta (Ehrh.) G. Gaertn., B. Mey. et Scherb. s. l.) are described from the Amur River basin (Amur Region or Khabarovsk Territory); Arundinella rossica Prob. (sect. Hirtae) and Calamagrostis kozhevnikovii Prob. et Prokopenko (sect. Calamagrostis) from Primorye Territory.


Zootaxa ◽  
2018 ◽  
Vol 4403 (2) ◽  
pp. 378 ◽  
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
DMITRY M. PALATOV

Illustrated descriptions of the adult male, pupa and fourth instar larva, as well as DNA barcoding results of Chaetocladius (Chaetocladius) elisabethae sp. nov. in comparison with closely related species of Chaetocladius s. str. from the Moscow Region are provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. Comparisons with corresponding regions of COI between C. (s. str.) elisabethae sp. nov. and other species of the subgenus produced K2P genetic distances of 0.11–0.16, values well associated with interspecific variation. The barcodes of the new species were identical to the Chaetocladius sp. 2ES in BOLD systems. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius s. str. 


Zootaxa ◽  
2018 ◽  
Vol 4472 (1) ◽  
pp. 153
Author(s):  
VALENTINA A. TESLENKO ◽  
LOUIS BOUMANS

Nemoura sirotskii sp. n. (Plecoptera, Nemouridae) is described as a new stonefly species from the tributary streams of Zeya Reservoir (Amur River Basin) in the south of the Russian Far East. Detailed descriptions and illustrations are provided for the larvae and adult specimens. The diagnostic characters distinguishing it from sympatric species N. arctica are discussed. 


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