Description of Pratylenchoides ojcowensis sp. nov. (Nematoda: Merlinidae) from Polish Jurassic Highland

A new species of the genus Pratylenchoides has been described. It was found in Polish Jurassic Highland, in Ojców National Park. Pratylenchoides ojcowensis sp. nov. was isolated from the soil located around tangled roots of Elymus sp. and Trifolium sp. This species is marked by a conical head in both females and males which is not separated from the body contour and has with 4–5 annuli; a relatively short stylet (20.3–21.3 µm females, 17.7–20.9 µm males) with oval knobs directed posteriorly; the dorsal pharyngeal nucleus located anterior to the cardia (the subventral pharyngeal nuclei located posterior; a pharyngeal lobe of length about two body widths (1.8–2.6); a lateral field with 6 lines in the middle part of body and sometimes with partially areolated outer bands; intestinal fasciculi present; round sperm in the spermatheca in females; a female tail with a maximum of 29 annuli, and an annulated tail terminus. The status of the new species has been verifiied by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The results obtained in the study indicated that P. ojcowensis sp. nov. is most related to P. alkani, P. ritteri and P. nevadensis from which is distinguished by the shape of the female head (conoid vs rounded), shorter stylet in females (20.3–21.3 µm vs 22.0–25.0 µm, 21.0–25.0 µm, 22.0–26.0 µm) and differences in 28S rDNA sequences. In addition (as per the original descriptions Yüksel 1977, Sher 1970, Talavera & Tobar 1996) it is distinguished from P. alkani by smaller number of male’s head annuli (4–5 vs 7–9), from P. ritteri it is distinguished by posteriorly directed stylet knobs (vs directed laterally), from P. nevadensis it is distinguished by oval and posteriorly directed stylet knobs (vs rounded and directed laterally).

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Generic classification of some more hyphomycetes with solitary conidia borne on phialides

Canadian Journal of Botany ◽

10.1139/b98-104 ◽

1998 ◽

Vol 76 (9) ◽

pp. 1570-1583 ◽

Cited By ~ 7

Author(s):

W Gams ◽

K O'Donnell ◽

H -J Schroers ◽

M Christensen

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Large Subunit ◽

Elongation Factor ◽

28S Rdna ◽

New Combination ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Three New Species ◽

Morphological Species

Unlike most phialide-producing fungi that liberate a multiplicity of conidia from each conidiogenous cell, only single conidia are formed on phialide-like conidiogenous cells in Aphanocladium, Verticimonosporium, and some species of Sibirina. A group of isolates obtained from soil of native Artemisia tridentata (sagebrush) grassland in Wyoming and from desert soil in Iraq is compared with these genera and classified as a fourth genus, Stanjemonium, honouring Stanley J. Hughes. Phylogenetic analyses of partial nuclear small- (18S) and large-subunit (28S) rDNA sequences indicate that Stanjemonium spp. form a monophyletic group with Emericellopsis. Sequences from the nuclear 18S and 28S rDNA were too conserved to resolve morphological species of Stanjemonium; however, phylogenetic analysis of b-tubulin and translation elongation factor 1a gene exons and introns resolved all species distinguished morphologically. Numerous conidiogenous cells or denticles are scattered along the cells of aerial hyphae in Aphanocladium and Stanjemonium spp., very rapidly collapsing into denticles in the former, somewhat more persistent and leaving broad scars in the latter. In Cladobotryum-Sibirina and Verticimonosporium spp., conidiogenous cells are discrete in terminal and intercalary whorls; phialides of the latter taxon are particularly swollen. The taxonomy of Aphanocladium is not yet resolved. Two species are recognized in Verticimonosporium. Three new species of Stanjemonium are described, and one new combination from Aphanocladium is proposed, along with one new species of Cladobotryum.Key words: Aphanocladium, Cladobotryum, conidiogenesis, hyphomycetes, molecular phylogeny, phialide, Stanjemonium, systematics, Verticimonosporium.

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Mexicana rubra sp. nov. and Encotyllabe cf. spari Yamaguti, 1934 (Monogenea) of Orthopristis ruber (Cuvier, 1830) from the Brazilian Coast off Rio de Janeiro

Helminthologia ◽

10.1515/helm-2017-0046 ◽

2017 ◽

Vol 54 (4) ◽

pp. 336-347

Author(s):

A. C. A. Camargo ◽

J. L. Luque ◽

C. P. Santos

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Rio De Janeiro ◽

Genetic Data ◽

28S Rdna ◽

Brazilian Coast ◽

Diagnostic Features ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Partial 18S

Summary Mexicana rubra sp. nov. and Encotyllabe cf. spari are described from the gills of the marine fish Orthopristis ruber (Haemulidae) caught off Rio de Janeiro, Brazil. Morphological, ultrastructural and genetic data are provided. The main diagnostic features of this new species of ectoparasite are a posteriorly bipartite testis, a ventral bar with three deep bowed projections and a dorsal bar with two deep, conspicuous, antero-lateral indentations. Genetic data on Mexicana rubra sp. nov. are based on the ITS1, 5.8S and partial 18S and 28S rDNA. This is the sixth known species of Mexicana Caballero & Bravo-Hollis, 1959, a key to which is also presented. Encotyllabe cf. spari Yamaguti, 1934 is described from the same host, with new ultrastructural data and new partial 18S and 28S rDNA sequences. A phylogenetic analysis based on partial 18S and 28S sequences is undertaken for both species.

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Description of Ektaphelenchus taiwanensis sp. n. (Nematoda: Ektaphelenchinae) found in packaging wood from Taiwan

Nematology ◽

10.1163/15685411-00002676 ◽

2013 ◽

Vol 15 (3) ◽

pp. 329-338 ◽

Cited By ~ 6

Author(s):

Jianfeng Gu ◽

Jiangling Wang ◽

Xianfeng Chen

Keyword(s):

New Species ◽

Phylogenetic Trees ◽

Morphological Characters ◽

Feeding Habit ◽

The Body ◽

Body Contour ◽

Lateral Field ◽

Male Tail

Ektaphelenchus taiwanensis sp. n. is described and figured. The new species was isolated from pine packaging wood from Taiwan and inspected in Ningbo harbour, China, in 2009. Ektaphelenchus taiwanensis sp. n. is characterised by the lip region lacking a clear constriction separating it from the body contour, stylet relatively short (12-17 μm) and without basal knobs, and presence of three lines in the lateral field. The female post-uterine sac is less than a body diam. long, the rectum and anus are absent and the tail is conoid with a sharply pointed terminus. The male tail is conoid and has a hair-like mucron (0.5-2.5 μm), the spicules are mitten-shaped, 12.5-14.4 μm long (chord) and are smoothly curved with a prominent rounded condylus, prominent pointed rostrum, and broadly rounded distal end. There are three pairs of subventral caudal papillae. Ektaphelenchus taiwanensis sp. n. is similar to E. betulae, E. josephi, E. joyceae, E. prolobos, E. propora and E. tuerkorum from which it can be separated by a combination of various morphometric and morphological characters. The D2D3 LSU and partial SSU region sequences were analysed and aligned using ClustalW implemented in MEGA version 4.0. Phylogenetic trees were generated with the Neighbour Joining (NJ) method using the Tajima-Nei distance option. The feeding habit of the new species is briefly discussed.

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A new species of Auriculostoma (Trematoda: Allocreadiidae) from the intestine of Brycon guatemalensis (Characiformes: Bryconidae) from the Usumacinta River Basin, Mexico, based on morphology and 28S rDNA sequences, with a key to species of the genus

Zootaxa ◽

10.11646/zootaxa.4196.2.5 ◽

2016 ◽

Vol 4196 (2) ◽

pp. 261 ◽

Cited By ~ 5

Author(s):

DAVID IVÁN HERNÁNDEZ-MENA ◽

CHRISTINA LYNGGAARD ◽

BERENIT MENDOZA-GARFIAS ◽

GERARDO PÉREZ-PONCE DE LEÓN

Keyword(s):

New Species ◽

Geographical Distribution ◽

Genetic Divergence ◽

28S Rdna ◽

The Other ◽

Sister Taxon ◽

Host Association ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

A New Species

We describe a new species of Auriculostoma Scholz, Aguirre-Macedo & Choudhury, 2004 based on several sources of information including morphology (light and scanning electron microscopy [SEM]), sequences of two nuclear genes, host association, and geographical distribution. Morphologically, the new species most closely resembles Auriculostoma astyanace Scholz, Aguirre-Macedo & Choudhury, 2004, but differs by having deeply lobated testes and cirrus-sac extending posteriorly to seminal receptacle level. Auriculostoma lobata n. sp. can be readily distinguished from all the other congeners by the combination of the following characters: testes located in tandem, testes deeply lobated, and larger body size. A phylogenetic analysis using 28S rDNA sequences along with those available for other allocreadiid trematodes, revealed that the new species is a sister taxon of A. astyanace, a species described from the banded astyanax, Astyanax fasciatus (Cuvier) in Nicaragua. Auriculostoma totonacapanensis Razo-Mendivil, Mendoza-Garfias, Pérez-Ponce de León & Rubio-Godoy, 2014 from the Mexican tetra, Astyanax mexicanus (De Filippi) in Mexico is the sister taxon of A. astyanace plus the new species. Genetic divergence levels for the 28S rDNA and ITS2 were estimated among the Middle-American species of Auriculostoma infecting characiforms. The validity of the new species is then established by reliable morphological differences, its host association to bryconids (Brycon guatemalensis Regan), restricted geographical distribution (Usumacinta and Lacantun River basins), and genetic divergence levels, albeit relatively low. A morphometric comparison between the new species and the other seven congeneric species was undertaken and, in addition, a taxonomic key to identify the species contained in the genus Auriculostoma, widely distributed across the Americas, is provided.

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Erysiphe deutziicola sp. nov. (Erysiphaceae, Ascomycota), a powdery mildew species found on Deutzia parviflora (Hydrangeaceae) with unusual appendages

MycoKeys ◽

10.3897/mycokeys.51.34956 ◽

2019 ◽

Vol 51 ◽

pp. 97-106

Author(s):

Peng-Lei Qiu ◽

Uwe Braun ◽

Yu Li ◽

Shu-Yan Liu

Keyword(s):

New Species ◽

Powdery Mildew ◽

Microscopic Examination ◽

Phylogenetic Analyses ◽

28S Rdna ◽

Bootstrap Support ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Beijing China ◽

Ornamental Shrub

A powdery mildew (Erysiphales) has recently been collected on leaves of an ornamental shrub Deutziaparviflora in Baihua Mountain, Beijing, China. Microscopic examination of the chasmothecia suggested a species belonging to Erysiphesect.Erysiphe, above all due to mycelioid chasmothecial appendages, although circinate apices of the appendages were rather in favour of Erysiphesect.Uncinula, which is a fairly rare combination of appendage characteristics in Erysiphe. Phylogenetic analyses of ITS and 28S rDNA sequences demonstrated that the two examined powdery mildew collections on D.parviflora clustered together as an independent lineage within Erysiphe with 100% bootstrap support, representing a species of its own, which is phylogenetically allied to, but clearly distinct from Erysiphedeutziae and, in addition, morphologically quite different from all known Erysiphe species on hosts belonging to the Hydrangeaceae. The new species on D.parviflora is described as Erysiphedeutziicola.

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Description of Hemicycliophora cardamomi sp. n. (Nematoda: Hemicycliophoridae) associated with Amomum longiligulare T.L. Wu and a web-based key for the identification of Hemicycliophora spp.

Journal of Helminthology ◽

10.1017/s0022149x20000966 ◽

2021 ◽

Vol 95 ◽

Author(s):

H.T. Nguyen ◽

Q.P. Trinh ◽

M. Couvreur ◽

T.D. Nguyen ◽

W. Bert

Keyword(s):

New Species ◽

Parasitic Nematode ◽

The Body ◽

Plant Diseases ◽

28S Rrna ◽

Body Contour ◽

Web Based ◽

Lateral Field ◽

Identification Process ◽

Tail Tip

Abstract During a survey of plant diseases in the Central Highlands of Vietnam, a new plant-parasitic nematode, Hemicycliophora cardamomi sp. n., was discovered in the growing areas of Amomum longiligulare, a valuable medicinal plant with high economical value. The new species is characterized by a cuticular sheath loosely fitting body; a labial region continuous to the body contour bearing 3 annuli; a lateral field frequently marked by a discontinuous single line with indistinct additional short lines in some parts along the body; a modified vulval lip with an anterior lip c. 4 annuli long; and a post-vulval body tapering to a pointed tail tip with an abrupt constriction at the last third of the post-vulval body. Morphology and molecular characterizations of D2-D3 expansion segment of 28S rRNA, ITS, and COI mtDNA gene regions clearly distinguish the new species from all other 133 known species. This study also provides a newly developed web-based key for the identification of Hemicycliophora spp. in order to accelerate the identification process, to minimize the increasing error load associated with larger datasets, and to avoid any dependence on a single starting entry. This key includes both an existing Bray–Curtis similarity measure and a newly developed similarity formula.

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Mucor cheongyangensis, a new species isolated from the surface of Lycorma delicatula in Korea

Phytotaxa ◽

10.11646/phytotaxa.446.1.4 ◽

2020 ◽

Vol 446 (1) ◽

pp. 33-42

Author(s):

THUONG T. T. NGUYEN ◽

HYANG BURM LEE

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Internal Transcribed Spacer ◽

Large Subunit ◽

28S Rdna ◽

Fungal Taxon ◽

Rdna Sequences ◽

Lycorma Delicatula ◽

28S Rdna Sequences ◽

A New Species

A new species of Mucor, isolated from the surface of Lycorma delicatula collected at Cheongyang in the Chungnam Province of Korea, is described and compared with morphologically similar taxa. The phylogenetic analysis of the internal transcribed spacer (ITS) and the large subunit (28S) rDNA sequences revealed that this isolate is closely related to M. orantomantidis and M. guilliermondii. However, the new isolate differs from those by having larger sporangia (24.5–125 × 23.5–120 µm), rhizoid-like structures, and production of secondary sporangia from vesicles outside of the original sporangium. Here, this novel fungal taxon is proposed as Mucor cheongyangensis sp. nov.

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Phylogenetic characterisation of seven Unicapsula spp. (Myxozoa: Myxosporea: Multivalvulida) from commercial fish in southern China and Japan

Parasitology ◽

10.1017/s0031182019001793 ◽

2019 ◽

Vol 147 (4) ◽

pp. 448-464 ◽

Cited By ~ 2

Author(s):

Ying-Chun Li ◽

Souhei Tamemasa ◽

Jin-Yong Zhang ◽

Hiroshi Sato

Keyword(s):

New Species ◽

Polar Capsule ◽

Type Species ◽

Southern China ◽

28S Rdna ◽

Commercial Fish ◽

Rdna Sequences ◽

Two New Species ◽

28S Rdna Sequences ◽

China And Japan

AbstractThe myxozoan genus Unicapsula Davis, 1924 (Myxosporea: Multivalvulida: Trilosporidae) is characterized as having one functional polar capsule (PC) and two rudimentary PCs in a three-valved myxospore. The plasmodia of Unicapsula spp. grow either in the myofibres or in the gills, oesophageal walls and urinary organs of marine fish. Few studies have investigated the taxonomy of Unicapsula spp. including the type species Unicapsula muscularis. Accordingly, the taxonomy of the genus was explored in the present study by using 15 new isolates of seven Unicapsula spp. (U. muscularis, U. galeata, U. andersenae, U. pyramidata, U. pflugfelderi, and two new species) that had formed pseudocysts in the trunk myofibres of commercial fish collected in southern China and Japan from November 2015 to January 2019. Two new species Unicapsula trigona n. sp., and Unicapsula motomurai n. sp. exhibited unique myxospore morphologies (semi-triangular and spherical myxospores, respectively) and 18S and 28S rDNA sequences that were distinct from those of the other Unicapsula spp. Phylogenetic analysis of the 18S and 28S rDNA sequences confirmed the monophyletic status of Unicapsula.

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Description of Calcaridorylaimus heynsi n. sp. (Nematoda: Dorylaimidae) from Potchefstroom, South Africa

Nematology ◽

10.1163/15685411-00003346 ◽

2020 ◽

Vol 22 (8) ◽

pp. 855-865

Author(s):

Antoinette Swart ◽

Hendrika Fourie ◽

Louwrens R. Tiedt ◽

Milad Rashidifard

Keyword(s):

South Africa ◽

Phylogenetic Analysis ◽

New Species ◽

18S Rdna ◽

Molecular Techniques ◽

28S Rdna ◽

Median Line ◽

Rdna Sequences ◽

28S Rdna Sequences ◽

Short Body

Summary Calcaridorylaimus heynsi n. sp. is the second species of the genus to be described by both morphological and molecular techniques. Morphologically, it can be distinguished from all known species of Calcaridorylaimus by a combination of the following characters: presence of advulval ornamentations, short body (0.90-1.33 mm), slightly anteriorly positioned vulva (V = 47.6 (45.8-49.8)), short odontostyle in females and males (13.1 (11.5-14.5) μm and 13.5 (12.0-18.0) μm, respectively), number of supplements (2 + 9-11), short spicules when measured along the median line (40.4 (38-42) μm) and pore-like vulval opening. It is closest to C. sirgeli, especially in the presence of advulval ornamentations and the pore-like vulva. Phylogenetic analysis based on partial D2-D3 segment of 28S rDNA sequences showed that C. heynsi n. sp. is in a well-supported sister relation with Mesodorylaimus sp. in a clade with C. cignatus and Mesodorylaimus spp. In the Bayesian tree, using partial sequences 18S rDNA, M. japonicus was the closest taxon to the new species.

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Dentipellis fimbriata sp. nov. (Russulales, Basidiomycota) from subtropical Taiwan

Phytotaxa ◽

10.11646/phytotaxa.428.2.7 ◽

2020 ◽

Vol 428 (2) ◽

pp. 131-138

Author(s):

CHIA-LING WEI ◽

CHIUNG-CHIH CHANG ◽

SHENG-HUA WU

Keyword(s):

New Species ◽

28S Rdna ◽

Key To Species ◽

Hyphal System ◽

Rdna Sequences ◽

Phylogenetic Studies ◽

28S Rdna Sequences

Dentipellis fimbriata sp. nov. is described and illustrated from Taiwan. This new species is characterized by odontoid to hydnoid hymenophore, margin with rhizomorphs, monomitic hyphal system with clamp connections, subiculum with yellowish brown hyphae, aculeal trama with numerous tubular gloeocystidia, and amyloid basidiospores. Morphological and phylogenetic studies based on ITS and 28S rDNA sequences, support this new species in Dentipellis. We provide a key to species of Dentipellis, Dentipellicula and Dentipellopsis.

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