scholarly journals Isolation and Characterization of Indole Acetic Acid Producing Halophilic Bacteria from Salt Affected Soil of Rice–Shrimp Farming System in the Mekong Delta, Vietnam

2017 ◽  
Vol 6 (3) ◽  
pp. 69 ◽  
Author(s):  
Nguyen Khoi Nghia
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Halophilic Bacteria ◽  
Mekong Delta ◽  
Farming System ◽  
Shrimp Farming ◽  
Salt Affected Soil ◽  
Isolation And Characterization

Isolation and Characterization of Indole Acetic Acid (IAA) Produced by a Halo Tolerant Marine Bacterium Isolated from Coastal Sand Dune Plants

2014 ◽  
Vol 11 (SE) ◽  
pp. 263-269
Author(s):  
M. Jayaprakashvel ◽  
K. Abishamala ◽  
C. Mathan Periasamy ◽  
J Satheesh ◽  
A. Jaffar Hussain ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Indole Acetic Acid ◽  
Marine Bacterium ◽  
Sand Dune ◽  
Coastal Sand Dune ◽  
Isolation And Characterization ◽  
Coastal Sand ◽  
Dune Plants

scholarly journals Isolation and Characterization of Halophilic Bacteria from Salinity Soil of Shatkhira, Bangladesh

2020 ◽  
pp. 67-76
Author(s):  
Moni Krishno Mohanta ◽  
Samima Nasrin ◽  
Md. Fazlul Haque ◽  
Alpona Sarker Hasi ◽  
Ananda Kumar Saha
Keyword(s):  
Soil Sample ◽  
Salt Concentration ◽  
Halophilic Bacteria ◽  
Bacillus Sp ◽  
Mineral Salts ◽  
Acinetobacter Junii ◽  
Salt Affected Soil ◽  
Isolation And Characterization ◽  
Acinetobacter Sp

Aim: The aim of this study was undertaken for the isolation and molecular characterization of the halophilic bacteria from salt affected soils. Place and Duration of Study: This study was conducted on the salinity affected area in Ramzan Nagor under Shamnagor Upazilla of Shatkhira District, Bangladesh. The collection of soil sample, isolation of halophilic bacteria and subsequent experiments were done from May to December 2018. Methodology: Salt affected soil could be good source of halophilic bacteria as it contain high amount of salt. Hence, one gram of salt affected soil sample which was containing high amount of salt was suspended to 100 ml sterile distilled water and one ml of sample from the top of the suspension were taken in to 250 ml Erlenmeyer flaks containing 100 ml of mineral salts (MS) medium. The primary enrichment was incubated for several days at 37°C with shaking at 120 rpm (revolution per minute) on an orbital shaker. Cultures which were found to be turbid after a period of up to 4 days were used as inocula in subsequent experiments. Results: Four halophilic bacterial strains viz. Bacillus sp. strain 8-15, Enterobacter sp. strain LCR75, Acinetobacter sp. strain 407 and Acinetobacter junii strain F27 were isolated from the saline soil and screened for their growth at a range of salt (NaCl) concentrations viz., 0%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% and 5%. Out of four isolates, Bacillus sp. showed the highest capacity to tolerate 5% salt concentration whereas Enterobacter sp. and other two isolates (Acinetobacter sp. and Acinetobacter junii) showed the capacity to tolerate 4% and 2.5% salt concentration respectively. Based on their morphological and biochemical characteristics the isolates were found to be Gram-positive and Gram-negative, rod shaped, motile bacteria which were later identified on the basis of 16S rRNA gene sequencing. The optimum culture conditions of the isolates were pH 7.0 and temperature 37°C. Results on the antibiogram profiles of the isolates revealed that the strains were sensitive to most of the antibiotics used in this study except Kenamycin, Ceftazidime and Ampicillin. Conclusion: The isolated halophilic bacteria may play an important role to optimize the salt affected soil; hence they could be important candidates for further research on slat bioremediation.

scholarly journals Isolation and Characterization of Indole Acetic Acid Producing Bacteria from Rhizosphere Soil and their Effect on Seed Germination

2019 ◽  
Vol 8 (03) ◽  
pp. 1237-1245 ◽  
Author(s):  
Sharnali Das ◽  
Tauhidur Rahman Nurunnabi ◽  
Rehana Parveen ◽  
Amatun Nur Mou ◽  
Md. Emdadul Islam ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Indole Acetic Acid ◽  
Rhizosphere Soil ◽  
Isolation And Characterization ◽  
Acid Producing Bacteria

scholarly journals Isolation and characterization of Anoxygenic phototrophs from shrimp ponds in Thailand

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Xelimar Ramirez ◽  
Imeleta Luamanu ◽  
Ruben Michael Ceballos ◽  
Elizabeth Padilla Crespo
Keyword(s):  
Environmental Samples ◽  
Production Systems ◽  
Phenotypic Diversity ◽  
Purple Bacteria ◽  
Shrimp Farming ◽  
Post Inoculation ◽  
Shrimp Ponds ◽  
Anoxygenic Phototrophs ◽  
Isolation And Characterization

Anoxygenic phototrophic purple bacteria are ubiquitous in aquatic and terrestrial environments and demonstrate broad phenotypic diversity. Purple bacteriaderive energy from light under anaerobic conditions via anoxygenic photosynthesis, a process in which water is not the electron donor. It has been suggested that these bacteria are useful for a variety of applications, including: wastewater treatment; heavy metal remediation; nitrogen fixation; and, control of CH4 emissions. In this study, the goal was to isolate and characterize PNSB from shrimp ponds in Thailand. Surface water and sediment were collected. Enrichment cultures were prepared using Pfenning’s mineral media. As indicated by development of reddish color and turbidity, anoxygenic phototrophic growth was observed within two days of incubation. Cultures in liquid media and on solid plates exhibited a deep red or purple color ten weeks post-inoculation. Under light microscopy, enrichments consist of communities dominated by thin, elongated gram-negative cells with granules of elemental sulfur, which are characteristic of purple bacteria. Molecular methods confirm the presence of pufLM, a genetic biomarker for purple bacteria (e.g., Thiohalocapsa marina, Allochromatium vinosum, Roseovarius tolerans). Initial sequencing of key genes (i.e., pufLM) indicate that these environmental samples contain novel isolates or “geographic variants” that have not been previously described. We have developed a few pure cultures of multiple species from these environmental samples. Since shrimp farming is a key industry in southern Thailand, the characterization of the microbial communities in these ecosystems, including anoxygenic phototrophs, will provide insights into how to maintain water quality in these food production systems.

scholarly journals Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming

2015 ◽  
Vol 43 (1) ◽  
pp. 123-136 ◽  
Author(s):  
Ana Claudia Sanchez Ortiz ◽  
Antonio Luna Gonzalez ◽  
Angel Isidro Campa Cordova ◽  
Ruth Escamilla Montes ◽  
Maria del Carmen Flores Miranda ◽  
...  
Keyword(s):  
Shrimp Farming ◽  
Probiotic Bacteria ◽  
Isolation And Characterization
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