scholarly journals Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming

2015 ◽  
Vol 43 (1) ◽  
pp. 123-136 ◽  
Author(s):  
Ana Claudia Sanchez Ortiz ◽  
Antonio Luna Gonzalez ◽  
Angel Isidro Campa Cordova ◽  
Ruth Escamilla Montes ◽  
Maria del Carmen Flores Miranda ◽  
...  
Keyword(s):  
Shrimp Farming ◽  
Probiotic Bacteria ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Anoxygenic phototrophs from shrimp ponds in Thailand

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Xelimar Ramirez ◽  
Imeleta Luamanu ◽  
Ruben Michael Ceballos ◽  
Elizabeth Padilla Crespo
Keyword(s):  
Environmental Samples ◽  
Production Systems ◽  
Phenotypic Diversity ◽  
Purple Bacteria ◽  
Shrimp Farming ◽  
Post Inoculation ◽  
Shrimp Ponds ◽  
Anoxygenic Phototrophs ◽  
Isolation And Characterization

Anoxygenic phototrophic purple bacteria are ubiquitous in aquatic and terrestrial environments and demonstrate broad phenotypic diversity. Purple bacteriaderive energy from light under anaerobic conditions via anoxygenic photosynthesis, a process in which water is not the electron donor. It has been suggested that these bacteria are useful for a variety of applications, including: wastewater treatment; heavy metal remediation; nitrogen fixation; and, control of CH4 emissions. In this study, the goal was to isolate and characterize PNSB from shrimp ponds in Thailand. Surface water and sediment were collected. Enrichment cultures were prepared using Pfenning’s mineral media. As indicated by development of reddish color and turbidity, anoxygenic phototrophic growth was observed within two days of incubation. Cultures in liquid media and on solid plates exhibited a deep red or purple color ten weeks post-inoculation. Under light microscopy, enrichments consist of communities dominated by thin, elongated gram-negative cells with granules of elemental sulfur, which are characteristic of purple bacteria. Molecular methods confirm the presence of pufLM, a genetic biomarker for purple bacteria (e.g., Thiohalocapsa marina, Allochromatium vinosum, Roseovarius tolerans). Initial sequencing of key genes (i.e., pufLM) indicate that these environmental samples contain novel isolates or “geographic variants” that have not been previously described. We have developed a few pure cultures of multiple species from these environmental samples. Since shrimp farming is a key industry in southern Thailand, the characterization of the microbial communities in these ecosystems, including anoxygenic phototrophs, will provide insights into how to maintain water quality in these food production systems.

scholarly journals Isolation and characterization of potential probiotic bacteria from jordanian traditional pickled and fermented foods

2021 ◽  
Vol 78 (4) ◽  
pp. 515-520
Author(s):  
Suha Abudoleh ◽  
Sara Hamdan ◽  
Adel Mahasneh ◽  
Zahira Al-Khani ◽  
Ahmad Talhouni
Keyword(s):  
Probiotic Bacteria ◽  
Fermented Foods ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Infectious Vibrio Species from the Whiteleg Shrimp: Litopenaeus vannamei

2020 ◽  
Vol 2 (1) ◽  
pp. 36
Keyword(s):  
Litopenaeus Vannamei ◽  
Economic Loss ◽  
Vibrio Species ◽  
Shrimp Farming ◽  
Microbial Risk Assessment ◽  
Microbial Risk ◽  
Isolation And Characterization ◽  
Vibrio Campbellii ◽  
Vibrio Spp

Aquaculture shrimp farming has serious problems with diseases caused by viruses and bacteria, which lead to severe economic loss. The genus Vibrio is a group of facultative anaerobic microbes, most frequently found in aquatic environments and marine hosts. The Gram-negative genus Vibrio is one of the most important classes of bacterial pathogens in aquaculture systems and is a key cause of high mortality. The isolation, identification, and molecular characterization of Vibrio spp are studied from infected shrimp Litopenaeus vannamei. The microorganisms were isolated from the tissues of a white gut infected shrimp collected from diseased aquaculture ponds. After subculturing, microbial isolates were obtained using TCBS agar plates for vibrio selection, and the biochemical key was developed. Meanwhile, 11 isolates of bacteria belonging to the Vibrionaceae family have been identified in this study. For these, six Vibrio species-related isolates are responsible for vibriosis in the shrimp. These included Vibrio harveyi, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio owensii, Vibrio rotiferianus, and Vibrio alginolyticus. The extraction of DNA was carried out with a QIAamp DNA Mini Kit (QIAGEN). The extracted DNA was analyzed using an electrophoresis of 1 % agarose gel, the consistency and quantity of Vibrio spp's gDNA. NanoDropTM 2000 spectrophotometer (Thermo Scientific) was used for determination. In addition, the Agilent 2100 Bioanalyzer (Agilent Technologies) has been used to verify the gDNA libraries' validity, purity, concentration, and scale. In addition, Vibrio spp, a phylogenetic tree focused on maximum likelihood and bootstrapping, was performed using the MEGA version to examine the evolutionary phylogenetic relationship between the strains. This study's main objective would be useful in integrating strain variation in predicting microbiology and microbial risk assessment and may provide scientific guidelines for major Vibrio strains that may be responsible for vibriosis infection in the management of shrimp aquaculture disease.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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