High Frequency of Multiple Shoots Induction in Paederia Foetida (L.)- A Rare Medicinal Plant

Plant ◽  
2013 ◽  
Vol 1 (5) ◽  
pp. 60 ◽  
Author(s):  
M. Thirupathi
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
Multiple Shoots ◽  
Paederia Foetida

scholarly journals In vitro Plant Regeneration from Axillary Buds of Asparagus racemosus Wild, a Medicinal Plant

1970 ◽  
Vol 45 (3) ◽  
pp. 255-260
Author(s):  
F Afroz ◽  
MAA Jahan ◽  
AKM Sayeed Hassan ◽  
R Khatun
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
Shoot Proliferation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Asparagus Racemosus ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Multiple Shoot Regeneration

An efficient method was developed for regeneration of Asparagus racemosus Wild, from axillary explants. MS media supplemented with different concentrations of cytokinin (BAP) alone or in combination of different concentrations of auxin (NAA or IBA) was tested for their efficiency in multiple shoot induction. High frequency of multiple shoot regeneration was achieved on MS medium supplemented with BAP (0.1mg/l) and NAA (0.05 mg/l). When shoots were well developed they were dissected and subcultured on the same medium to promote more multiple shoots. Eighteen to twenty shoots were obtained on this medium. The shoots were rooted best on half MS medium supplemented with 0.05 mg/l BAP and 1.0 mg/l IBA. Among the five levels of pH tested, 5.7 was the best for multiple shoot proliferation. The result presented here proved to be suitable for the rapid propagation system of A. racemosus. Key words: Asparagus racemosus; Medicinal plant; Rapid proliferation; Multiple shoots; Auxins and Cytokinins DOI: 10.3329/bjsir.v45i3.6534Bangladesh J. Sci. Ind. Res. 45(3), 255-260, 2010

scholarly journals Seaweed Extract Promotes High-Frequency in vitro Regeneration of Solanum surattense Burm.f:A Valuable Medicinal Plant

2017 ◽  
Vol 11 (4) ◽  
pp. 134-141
Author(s):  
Packiaraj Gurusarava ◽  
Sadasivam Vinoth ◽  
Ganesan Prem Kumar ◽  
Pandiselvi .
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Seaweed Extract ◽  
Solanum Surattense

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

scholarly journals 893 PB 499 HIGH FREQUENCY REGENERATION FROM THE LEAF OF CAPSICUM ANNUUM L. CV. CHUNGHONG

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 561g-562
Author(s):  
Young Goel Shon ◽  
Joong Choon Park ◽  
Byoung Ryong Jeong
Keyword(s):  
Capsicum Annuum ◽  
Growth Regulators ◽  
High Frequency ◽  
Agar Medium ◽  
Multiple Shoots ◽  
Pepper Plant ◽  
Glass Bottle ◽  
Regeneration Frequency ◽  
Capsicum Annuum L ◽  
Better Than

Effect of combination and concentration of growth regulators on the regeneration of pepper plant from different explant tissues was studied. Seedlings were grown aseptically in 400 ml glass bottles containing MS agar medium at 26±2C under a 16 h·d-1 photoperiod (2000 lux, florescent lamps). Explants taken from 4 week-old seedlings were cultured under these conditions on 40 ml of MS agar (8 g·liter-1) medium containing 3 g·liter-1 sucrose in a 400 ml glass bottle. Primary and subsequent leaves attached to petiole regenerated better than cotyledon and hypocotyl. Among the combinations of different concentrations of cytokinin and auxin added in the medium, a combination of 5 μM IAA with either 10 μM zeatin or 10 μM BA gave the best regeneration. With these combinations, regeneration frequency of multiple shoots from the primary and subsequent leaves was greater than 70%. Regenerated shoots rooted readily in MS agar medium containing 3 g·liter-1 sucrose and 0.5 μM NAA.

scholarly journals Micropropagation of White Palash tree (Butea monosperma (Lam.) Taub. Var. lutea (Witt.)).

2017 ◽  
Vol 9 ◽  
Author(s):  
Rathnaprabha D ◽  
Muralikrishna N ◽  
Raghu E ◽  
Yashodhara V ◽  
Sadanandam A
Keyword(s):  
High Frequency ◽  
Multiple Shoots ◽  
Time Interval ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Regeneration Medium ◽  
Butea Monosperma ◽  
Cotyledonary Nodes ◽  
Mature Seeds

<p class="Default"><span>An efficient and reproducible protocol is established for rapid <em>in vitro</em> multiplication of an endangered, valuable medicinal plant, <em>Butea monosperma </em>(Lam.) Taub. Var. <em>lutea</em>, through cotyledonary nodes of mature seeds. Among various cytokinins tested, high frequency of direct shoot regeneration was induced on Murashige and skoog (MS) medium supplemented with BAP, which found to be more effective and showed optimal response at 2 mg/L with a maximum number of </span><span>8.35±0.32 multiple shoots per explant. Proliferation of shoots was established by repeated subculturing on to same regeneration medium with 2-3 weeks of time interval. Rooting of regenerated shoots was achieved after 3 weeks of culture on MS medium containing 1 mg/L IBA. <em>In vitro</em> raised plantlets were transferred to pots containing sterilized soil and vermiculate mixture in 1:1 ratio and then shifted to greenhouse. Well established plantlets exhibited 75% survival rate.</span></p>

scholarly journals In Vitro Regeneration of Vitex negundo L., a Woody Valuable Medicinal Plant through High Frequency Axillary Shoot Proliferation

1970 ◽  
Vol 43 (3) ◽  
pp. 345-352 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
John Liton Munshi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Vitex Negundo ◽  
Axillary Shoot Proliferation

An efficient protocol was established for rapid and large scale propagation of woody aromatic medicinal plant Vitex negundo L. by in vitro shoot multiplication from shoot tips and nodal segments of mature plant. Of the four different growth regulators BA, Kn, GA3, NAA and coconut water, MS fortified with BA 1.0 mg/l was found to be the most effective for inducing multiple shoots from nodal explants. The percentage (96%) of shoot multiplication per node (21.83) was highest up to second subculture passages, after which there was a gradual decline in shoot development. Best rooting was induced (93%) in excised shoots on half strength MS medium supplemented with an optimal combination of NAA (0.3 mg/l). Soil, compost and sand (1:1:1) mixture was the most suitable planting substrate for hardening. The survival rate was 80% and the regenerated plants were successfully transferred to the soil.Key words: Vitex negundo, Medicinal plant, Shoot proliferation, Micropropagation, RegenerationDOI = 10.3329/bjsir.v43i3.1149Bangladesh J. Sci. Ind. Res. 43(3), 345-352, 2008

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