Construction of Repulsive Guidance Molecule A-Specific RNA Interference Recombinant Adenovirus

2019 ◽  
Vol 9 (10) ◽  
pp. 1369-1375
Author(s):  
Xiao Wang ◽  
Yong Zhang ◽  
Xiang-Dong Sun ◽  
Jing-Liang Cheng
Keyword(s):  
Rna Interference ◽  
Pc12 Cells ◽  
Recombinant Adenovirus ◽  
Transfection Efficiency ◽  
Rt Pcr ◽  
Subsequent Research ◽  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Guidance Molecule ◽  
Target Plasmid

This study aims to construct an RGMa RNAi plasmid in preparation for a subsequent research, and three shRNAs were designed for collection. The designed shRNAs were taken into the target plasmid of pegensil-1 to silence the mRNA of RGMa. Sequencing and enzyme identification were performed, and PC12 cells were transfected with a shRNA-containing plasmid. Then, the effect of the silencing of the gene was tested by RT-PCR using PC12 cells and to choose the best one. The results showed that the first one was choose for the subsequent research among these three designed shRNAs, which had the highest transfection efficiency and a concentration of 2.5 × 109 pfu/ml. The concentration of Adv(–) was 2.1 × 109 pfu/ml for the blank control. The effect of the constructed RGMa RNAi plasmid for the subsequent research is stable and effective.

scholarly journals Repulsive Guidance Molecule A Regulates Adult Neurogenesis Via the Neogenin Receptor

2020 ◽  
Vol 15 ◽  
pp. 263310552094848
Author(s):  
Toke Jost Isaksen ◽  
Toshihide Yamashita
Keyword(s):  
Adult Neurogenesis ◽  
Neuronal Development ◽  
Neurite Growth ◽  
Granular Cell ◽  
Molecular Networks ◽  
Mammalian Brain ◽  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Newborn Neurons ◽  
Guidance Molecule

Repulsive guidance molecule A (RGMa) exhibits repulsive guidance of axonal growth and regulates neuronal differentiation during development of the mammalian brain. In this commentary, we describe the findings of our recent paper, “Repulsive Guidance Molecule A Suppresses Adult Neurogenesis,” and discuss a possible model for RGMa suppression of newborn neurons that fail to properly migrate into the granular cell layer. In the study, we provided evidence that RGMa suppressed neurite growth and survival of newborn neurons in the adult dentate gyrus. This effect depends on the multifunctional Neogenin receptor expressed in adult neural stem cells through activation of the Rho-associated protein kinase leading to neurite growth inhibition and ultimately cell death. It should be noted that both RGMa and Neogenin interact with several well-described molecules, including bone morphogenetic proteins, that regulate neuronal development. Thus, it is likely that RGMa interacts with other intricate molecular networks that regulate adult neurogenesis.

scholarly journals LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin

2008 ◽  
Vol 107 (2) ◽  
pp. 418-431 ◽  
Author(s):  
Gregor Schaffar ◽  
Junko Taniguchi ◽  
Tanja Brodbeck ◽  
Axel H. Meyer ◽  
Martin Schmidt ◽  
...  
Keyword(s):  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Guidance Molecule

Repulsive guidance molecule a regulates hippocampal mossy fiber branching in vitro

Neuroreport ◽  
2013 ◽  
Vol 24 (11) ◽  
pp. 609-615 ◽  
Author(s):  
Kazuki Shibata ◽  
Soichiro Nakahara ◽  
Emi Shimizu ◽  
Toshihide Yamashita ◽  
Norio Matsuki ◽  
...  
Keyword(s):  
Mossy Fiber ◽  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Guidance Molecule

scholarly journals Targeting Repulsive Guidance Molecule A to Promote Regeneration and Neuroprotection in Multiple Sclerosis

Cell Reports ◽  
2015 ◽  
Vol 10 (11) ◽  
pp. 1887-1898 ◽  
Author(s):  
Elena Demicheva ◽  
Yi-Fang Cui ◽  
Philip Bardwell ◽  
Stefan Barghorn ◽  
Martina Kron ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Guidance Molecule

scholarly journals Repulsive guidance molecule A (RGMa)

2012 ◽  
Vol 6 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Brian Key ◽  
Grace J. Lah
Keyword(s):  
Repulsive Guidance Molecule ◽  
Repulsive Guidance Molecule A ◽  
Guidance Molecule
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