Correlation of N-Ras Gene Expression in Hepatocellular Carcinoma with Color Doppler Ultrasound Findings

2021 ◽  
Vol 11 (10) ◽  
pp. 1996-2000
Author(s):  
Yuguo Wei ◽  
Donglai Lv
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Western Blotting ◽  
Color Doppler ◽  
Color Doppler Imaging ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Ras Gene ◽  
Significant Difference ◽  
Tissue Specimens

Objective: This study aims to study the N-ras gene expression in hepatocellular carcinoma (HCC) and its correlation with color Doppler imaging manifestations. Methods: The tumors of 145 HCC patients were evaluated via color Doppler ultrasonography before operation and N-ras level in tissue specimens obtained after operation were detected via RT-PCR, Western blotting and immunohistochemistry along with correlation analysis. Results: N-ras mRNA in para-carcinoma tissues was significantly reduced compared with carcinoma tissues (P < 0.05). Western blotting and immunohistochemistry showed a significant difference of N-ras protein level between para-carcinoma tissues and carcinoma tissues (P < 0.05). Moreover, N-ras gene expression was siginificantly correlated with the number of tumor nodules, the integrity of tumor capsule, vascular invasion, blood flow classification, RI (P < 0.05). Conclusion: Our data demonstrate the elevation of N-ras in the carcinoma tissues of HCC patients and its correlation with color Doppler imaging manifestations, which lays foundation for the development of the diagnosis of hepatocellular carcinoma.

Color transcranial “power” Doppler ultrasound of intracranial aneurysms

1996 ◽  
Vol 84 (3) ◽  
pp. 459-461 ◽  
Author(s):  
Joanna M. Wardlaw ◽  
James C. Cannon
Keyword(s):  
Doppler Ultrasound ◽  
Intracranial Aneurysms ◽  
Flow Direction ◽  
Color Doppler ◽  
Power Doppler ◽  
Color Doppler Imaging ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Ultrasound Technique ◽  
Content Type

✓ “Color Doppler energy” (or “power Doppler”), a new color Doppler ultrasound technique that is independent of flow direction and very sensitive to movement, was assessed for its use in the identification of intracranial aneurysms in patients with recent subarachnoid hemorrhage immediately prior to using cerebral angiography. Features that identified aneurysms using this technique included the appearance of abnormal color where no normal artery was expected, abnormal bulging of an artery, and greater “expansibility” of the aneurysm in comparison to an adjacent normal vessel. In this exploratory study, 30 of 33 aneurysms were correctly identified in 35 patients with a good bone window. Color Doppler energy is considerably more sensitive to intracranial blood flow than conventional color Doppler imaging. Color Doppler energy is a useful research tool; if these preliminary results are verified in larger series, in addition to examination for vasospasm, the technique could be used for identification and follow up of aneurysms.

The efferent blood flow of early hepatocellular carcinoma and borderline lesions: Demonstration by color Doppler imaging

2002 ◽  
Vol 29 (4) ◽  
pp. 205-209 ◽  
Author(s):  
Hitoshi Tochio ◽  
Syusuke Tomita ◽  
Masatoshi Kudo ◽  
Nobuhiro Iwasaki ◽  
Syuji Tamura ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Blood Flow ◽  
Color Doppler ◽  
Color Doppler Imaging ◽  
Doppler Imaging ◽  
Early Hepatocellular Carcinoma

scholarly journals Comparison of Color Doppler Imaging Criteria for the Ophthalmic Artery and Central Retinal Artery in Infants with Retinopathy of Prematurity with and Without Plus Disease

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
Marzieh Soleimani ◽  
Seyed Ehsan Ghetmiri ◽  
Mohammad Hossein Ahrar Yazdi
Keyword(s):  
Retinopathy Of Prematurity ◽  
Ophthalmic Artery ◽  
Color Doppler ◽  
Resistance Index ◽  
Color Doppler Imaging ◽  
Central Retinal Artery ◽  
Doppler Imaging ◽  
Significant Difference ◽  
Retinal Artery ◽  
The Mean

Background: Color Doppler imaging (CDI) is a non-aggressive and safe technique for the clinical management of retinal diseases. Recently, the number of infants with retinopathy of prematurity (ROP) has increased due to the incidence of premature births. Objectives: This study aimed to compare the CDI criteria for the ophthalmic artery (OA) and central retinal artery (CRA) in ROP infants with and without plus disease. Patients and Methods: In this case-control study, 42 premature infants (21 infants with plus disease and 21 infants without plus disease) underwent CDI. The arterial CDI parameters, including end-diastolic velocity (EDV), pulsatility index (PI), resistance index (RI), and peak systolic velocity (PSV), were measured in these patients. After collecting the data, t-test and chi-square tests were used for data analysis. P < 0.05 was considered to be statistically significant. Results: The mean EDV of CRA in patients with and without plus disease was 4.35 ± 1.00 and 5.27 ± 1.02 cm/sec, respectively (P = 0.005). The mean PSV of CRA in patients with and without plus disease was 15.65 ± 3.35 and 18.39 ± 4.39 cm/sec, respectively (P = 0.029). However, no significant difference was found between the two groups in terms of PSV or EDV of OA (P > 0.05). Also, no significant difference was observed between the two groups considering the RI and PI of CRA and OA (P > 0.05). Conclusion: According to the findings of this study, CDI criteria, such as EDV and PSV of CRA, were significantly lower in infants with plus disease as compared to those without plus disease. Since detecting the early stages of plus disease is a challenge for ophthalmologists, assessment of these criteria can be helpful for differentiation of these two subgroups of patients. However, further studies with a larger sample size are needed to determine the cutoff value.

IMO 1801 The effect of contrast agent for the diagnosis of hepatocellular carcinoma on color doppler imaging

1997 ◽  
Vol 23 ◽  
pp. S33
Author(s):  
Seigo Sakaguchi ◽  
Keiji Tohara ◽  
Yasushi Miyajima ◽  
Masahiko Tanaka ◽  
Tomoko Mitsuyasu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Contrast Agent ◽  
Color Doppler ◽  
Color Doppler Imaging ◽  
Doppler Imaging

scholarly journals Evaluation of Corpus Luteum Vascularization in Recipient Mares by Using Color Doppler Ultrasound

2021 ◽  
Vol 49 ◽  
Author(s):  
Marlon De Vasconcelos Azevedo ◽  
Natália Matos Souza ◽  
Felipe Augusto Boudoux Martins Sales ◽  
José Carlos Ferreira-Silva ◽  
Maiana Silva Chaves ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Embryo Transfer ◽  
Doppler Ultrasound ◽  
Color Doppler ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Panicum Maximum ◽  
Progesterone Concentration ◽  
Vascular Perfusion ◽  
Significant Difference

Background: Embryo transfer is one of the most commonly used reproductive biotechnique. The success of embryo transfer is also affected by the synchrony of estrus and ovulation between donor and recipient animals. In horse reproduction, ultrasonography has been used, among other purposes, to diagnose early pregnancy. However, only the color Doppler imaging mode makes it possible to evaluate the vascular architecture and the hemodynamic aspects of the vessels in several organs, especially the corpus luteum. The objective of this study was to evaluate, based on the color Doppler ultrasound, the corpus luteum vascularization and function from recipient mares at embryo transfer timing.Materials, Methods & Results: Mangalarga Machador mares from 5 to 10-year-old and a range of live weights of between 350 to 450 kg were used for this experiment, kept in pasture-based on mombaça grass (Panicum maximum) and were given ad libitum access to water and mineral supplementation. The animals (n = 15) were gynecologically examined and uterine consistency was evaluated by rectal palpation the same operator using an ultrasound system (SonoScape®) with a linear transducer, and operating frequency ranging from 5 to 10 Mhz. The uterine tone was classified between grades 1 and 4 and subjected to ovulation induction. The objective and subjective vascular perfusion of the corpus luteum was evaluated by color Doppler ultrasound on the day of embryo transfer and endometrium. The determination progesterone concentration on the day of the embryo transfer was performed by direct chemiluminescence assay. The arcsine (√P/100) transformation was applied to the percentage data, and the results were expressed as mean (.) ± standard error of the mean (SEM). Further, the assumptions of normality and homoscedasticity were verified, respectively, based on the Shapiro-Wilk and Lilliefors tests. Regarding the parametric and non-parametric variables, were applied, respectively, analysis of variance (ANOVA) followed by Tukey’s test, and the Kruskal-Wallis test followed by Dunn’s test. Pearson’s correlation coefficient was used to evaluate the relationship between the parameters. The statistical program SPSS 16.0 was used to perform the over-mentioned analyses, and a p-value < 0.05 was taken as significant. Corpus luteum vascular perfusion, based on the objective and subjective evaluation methods, and the progesterone concentration were higher in the pregnant mares (P < 0.05). The objective and subjective methods for evaluation of the vascular perfusion in the corpus luteum were positively correlated between themselves as well as to progesterone concentration (P < 0.05). There was no significant difference between the groups considering the uterine tonus evaluation (P > 0.05).Discussion: Mares that later became pregnant showed a higher concentration of progesterone as an outcome of the higher vascularization in the corpus luteum. It can be supported by both the correlation between the progesterone concentration and the corpus luteum vascular perfusion, as well as by the higher values of the vascular perfusion in pregnant mares. Based on the results, it has been concluded that the color Doppler ultrasound evaluation is an accurate tool to determine the corpus luteum vascularization, whether considering the objective or subjective methods. Also, the vascular perfusion is the most efficient parameter to determine both the corpus luteum function and to predict the ability of the recipient mares to maintain pregnancy.

Color Doppler Imaging Study in Patients with Primary Open-Angle Glaucoma Treated with Timolol 0.5% and Carteolol 2%

2001 ◽  
Vol 11 (3) ◽  
pp. 240-244 ◽  
Author(s):  
P. Montanari ◽  
P. Marangoni ◽  
A. Oldani ◽  
R. Ratiglia ◽  
M. Raiteri ◽  
...  
Keyword(s):  
Intraocular Pressure ◽  
Primary Open Angle Glaucoma ◽  
Open Angle Glaucoma ◽  
Color Doppler ◽  
Resistance Index ◽  
Color Doppler Imaging ◽  
Doppler Imaging ◽  
Open Angle ◽  
Significant Difference ◽  
The Mean

Purpose To evaluate with color Doppler imaging (CDI), in patients with primary open-angle glaucoma (PDAG), the possible influence on ocular hemodynamics of a beta-blocking agent with intrinsic sympathomimetic acitivity (carteolol 2%) compared to a beta-blocker agent without this activity. Methods A study was carried out on 20 patients, with bilateral POaG, intraocular pressure (IOP) ≤ 20 mmHg, all treated twice a day with timolol maleate 0.5% ophthalmic solution. The visual field was evaluated (Octopus 2000 perimeter, G1 program) examining the mean sensitivity (MS) and the mean defect (MD). CDI was carried out to evaluate the resistance index of the internal carotid artery (ICA), the ophthalmic artery (OA), the central retinal artery (CRA), and the short posterior ciliary arteries (SPCA). After these examinations, the therapy was changed to carteolol 2% twice a day. After six months of treatment the examinations were repeated. The data were analysed statistically using Student's t test. Results The mean intraocular pressure during treatment with timolol 0.5% was 16.7 ± 1.67 mmHg and 16.33 ± 1.72 mmHg after treatment with carteolol 2%, the difference not being significant (p=0.494). After six months of treatment with carteolol 2% the MS increased significantly from 22.4 ± 2.5 dB to 24.1 ± 1.8 dB (p=0.018), and the mean defect (MD) fell from 5.3 ± 0.8 dB to 4.7 ± 0.6 dB (p=0.011). There was no significant difference in the resistance index of the CA, the OA and the CRA with the two treatments, whereas the resistance index of the SPCA dropped significantly, from 0.80 ± 0.05 to 0.77 ± 0.02 (p = 0.017). Conclusions CDI did not show significant differences in the resistance indexes of the internal CA, the OA, and the CRA after treatment with carteolol 2% but the resistance index of the SPCA was significantly reduced. Carteolol 2% induced significant changes in the perimetric indexes examined, with an increase in MS and a decrease in MD. These findings suggest that the intrinsic sympathomimetic activity of carteolol may reduce peripheral vascular resistance of the SCA, thus improving perfusion of the optic nerve head, with a protective effect on visual function.

Color-Doppler Ultrasound in Ovarian Masses: Anatomo-Pathologic Correlation

1993 ◽  
Vol 79 (4) ◽  
pp. 262-267 ◽  
Author(s):  
Carlo Spreafico ◽  
Laura Frigerio ◽  
Rodolfo Lanocita ◽  
G. Battista Spatti ◽  
Alfonso Marchianò ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Color Doppler ◽  
Resistance Index ◽  
Color Doppler Imaging ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Malignant Neoplasms ◽  
Malignant Lesions ◽  
Definition Of ◽  
Ovarian Masses

Background and aims In the radiologic assessment of ovarian masses, the major difficulty consists in the late recognition and lack of parameters for a differential diagnosis between benign and malignant lesions, especially in the post-menopause when the incidence of cancer is higher. The use of a transvaginal probe and the color-Doppler examination have recently improved the study of the female pelvis. This study is aimed to verify the possibility of the color-Doppler imaging to differentiate between malignant and benign ovarian lesions during transvaginal echographies, on the basis of the qualitative and quantitative characteristics of the vascular pattern of the ovarian lesions. Results Twenty-six expansive ovarian lesions were studied: 8/26 showed no vascular signals and were considered benign as confirmed at histology. In the remaining lesions with some vascularization, the resistance index (RI) was evaluated: those with RI > 0.40 were considered benign, those with RI < 0.40 malignant. In 8/9 benign lesions and in 7/9 malignant neoplasms, the results of color-Doppler were coherent with histology. The results showed a sensibility of 87.5 % and a specificity of 88.8 % for the transvaginal examination. Conclusions The main advantages of the color-Doppler transvaginal examination are: the high frequency of visualization of the ovaries, even in postmenopausal patients; the definition of small lesions; the visualization of small parenchymal vessels, both physiologic and pathologic, and their quantitative analysis. The importance of the RI cutoff was critical for the differential diagnosis between benign and malignant lesions: we think that a cutoff of 0.50, instead of 0.40 proposed by other authors, would be far more appropriate.

scholarly journals Color Doppler Evaluation of Arterial Resistive Index in Infantile Hemangioma: A Useful Parameter to Monitor the Response to Oral Propranolol?

2021 ◽  
Vol 9 ◽  
Author(s):  
George Koshy Parapatt ◽  
Teresa Oranges ◽  
Guglielmo Paolantonio ◽  
Lucilla Ravà ◽  
Simona Giancristoforo ◽  
...  
Keyword(s):  
Color Doppler ◽  
Resistive Index ◽  
Color Doppler Imaging ◽  
Infantile Hemangioma ◽  
Vascular Tumor ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Treatment Control ◽  
The Mean ◽  
Oral Propranolol

Infantile hemangioma (IH) is the most common benign vascular tumor in childhood. In more than 85% of all cases, IHs undergo spontaneous involution, but nearly 10–12% of IHs develop complications and require immediate therapy. Oral propranolol is currently the first-line treatment for IHs. Color Doppler ultrasound is the gold standard in the diagnosis of deep IH, and it is used to evaluate the morphological change and the modification of vascularization that occur during its evolution and treatment. To date, only few data in the literature described the changes of intralesional arterial resistive index (RI) during treatment with propranolol; particularly, some authors have shown an increase of intralesional arterial RI in IHs with clinical regression during treatment with propranolol. The objective of this paper is to evaluate the changes of RI of the intralesional arteries of the IHs during the treatment with oral propranolol. We retrospectively analyzed a total of 64 IHs in 60 patients treated with oral propranolol with a good clinical response. Gray-scale ultrasonography and color Doppler imaging were performed before and during the therapy. The intralesional RIs were measured before and during the treatment. For each lesion, we recorded the RI values, and then we calculated the mean RI value for any single lesion. We compared the mean RI value observed at the baseline with the mean RI value of the last detectable sampling at color Doppler. We also compared between them the mean RI values observed during intermediate ultrasound. The RI values were compared in 44 lesions, with at least two significant samplings of RI. In the 44 lesions compared, we did not find statistically significant variations in the mean RI values between the baseline control and the values recorded at the last post-treatment control. The time trend of mean RI values of the intermediate color Doppler analysis performed between the first pre-treatment control and the last measurable control did not show any statistically significant variation in the trend of mean RI values. Contrarily to what has been described by some authors, in our experience, we have not observed an increase of RI in IHs treated with oral propranolol.

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