scholarly journals Evaluation of Corpus Luteum Vascularization in Recipient Mares by Using Color Doppler Ultrasound

2021 ◽  
Vol 49 ◽  
Author(s):  
Marlon De Vasconcelos Azevedo ◽  
Natália Matos Souza ◽  
Felipe Augusto Boudoux Martins Sales ◽  
José Carlos Ferreira-Silva ◽  
Maiana Silva Chaves ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Embryo Transfer ◽  
Doppler Ultrasound ◽  
Color Doppler ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Panicum Maximum ◽  
Progesterone Concentration ◽  
Vascular Perfusion ◽  
Significant Difference

Background: Embryo transfer is one of the most commonly used reproductive biotechnique. The success of embryo transfer is also affected by the synchrony of estrus and ovulation between donor and recipient animals. In horse reproduction, ultrasonography has been used, among other purposes, to diagnose early pregnancy. However, only the color Doppler imaging mode makes it possible to evaluate the vascular architecture and the hemodynamic aspects of the vessels in several organs, especially the corpus luteum. The objective of this study was to evaluate, based on the color Doppler ultrasound, the corpus luteum vascularization and function from recipient mares at embryo transfer timing.Materials, Methods & Results: Mangalarga Machador mares from 5 to 10-year-old and a range of live weights of between 350 to 450 kg were used for this experiment, kept in pasture-based on mombaça grass (Panicum maximum) and were given ad libitum access to water and mineral supplementation. The animals (n = 15) were gynecologically examined and uterine consistency was evaluated by rectal palpation the same operator using an ultrasound system (SonoScape®) with a linear transducer, and operating frequency ranging from 5 to 10 Mhz. The uterine tone was classified between grades 1 and 4 and subjected to ovulation induction. The objective and subjective vascular perfusion of the corpus luteum was evaluated by color Doppler ultrasound on the day of embryo transfer and endometrium. The determination progesterone concentration on the day of the embryo transfer was performed by direct chemiluminescence assay. The arcsine (√P/100) transformation was applied to the percentage data, and the results were expressed as mean (.) ± standard error of the mean (SEM). Further, the assumptions of normality and homoscedasticity were verified, respectively, based on the Shapiro-Wilk and Lilliefors tests. Regarding the parametric and non-parametric variables, were applied, respectively, analysis of variance (ANOVA) followed by Tukey’s test, and the Kruskal-Wallis test followed by Dunn’s test. Pearson’s correlation coefficient was used to evaluate the relationship between the parameters. The statistical program SPSS 16.0 was used to perform the over-mentioned analyses, and a p-value < 0.05 was taken as significant. Corpus luteum vascular perfusion, based on the objective and subjective evaluation methods, and the progesterone concentration were higher in the pregnant mares (P < 0.05). The objective and subjective methods for evaluation of the vascular perfusion in the corpus luteum were positively correlated between themselves as well as to progesterone concentration (P < 0.05). There was no significant difference between the groups considering the uterine tonus evaluation (P > 0.05).Discussion: Mares that later became pregnant showed a higher concentration of progesterone as an outcome of the higher vascularization in the corpus luteum. It can be supported by both the correlation between the progesterone concentration and the corpus luteum vascular perfusion, as well as by the higher values of the vascular perfusion in pregnant mares. Based on the results, it has been concluded that the color Doppler ultrasound evaluation is an accurate tool to determine the corpus luteum vascularization, whether considering the objective or subjective methods. Also, the vascular perfusion is the most efficient parameter to determine both the corpus luteum function and to predict the ability of the recipient mares to maintain pregnancy.

Color transcranial “power” Doppler ultrasound of intracranial aneurysms

1996 ◽  
Vol 84 (3) ◽  
pp. 459-461 ◽  
Author(s):  
Joanna M. Wardlaw ◽  
James C. Cannon
Keyword(s):  
Doppler Ultrasound ◽  
Intracranial Aneurysms ◽  
Flow Direction ◽  
Color Doppler ◽  
Power Doppler ◽  
Color Doppler Imaging ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Ultrasound Technique ◽  
Content Type

✓ “Color Doppler energy” (or “power Doppler”), a new color Doppler ultrasound technique that is independent of flow direction and very sensitive to movement, was assessed for its use in the identification of intracranial aneurysms in patients with recent subarachnoid hemorrhage immediately prior to using cerebral angiography. Features that identified aneurysms using this technique included the appearance of abnormal color where no normal artery was expected, abnormal bulging of an artery, and greater “expansibility” of the aneurysm in comparison to an adjacent normal vessel. In this exploratory study, 30 of 33 aneurysms were correctly identified in 35 patients with a good bone window. Color Doppler energy is considerably more sensitive to intracranial blood flow than conventional color Doppler imaging. Color Doppler energy is a useful research tool; if these preliminary results are verified in larger series, in addition to examination for vasospasm, the technique could be used for identification and follow up of aneurysms.

scholarly journals COLOR DOPPLER ULTRASONOGRAPHY FINDINGS IN KIDNEY ALLOGRAFTS BEFORE AND AFTER TRANSPLANTATION

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Suraj Mathur ◽  
P. Rajan ◽  
Dr. Jaya Kumar E. K
Keyword(s):  
Doppler Ultrasound ◽  
Doppler Ultrasonography ◽  
Color Doppler ◽  
Vascular Complications ◽  
Biochemical Parameter ◽  
Color Doppler Ultrasound ◽  
Color Doppler Ultrasonography ◽  
Kidney Allograft ◽  
Significant Difference

Method: A sample size of 40 kidney allograft recipients undergoing color Doppler Ultrasonography evaluation included in the study. Result: Corticomeduallry differentiation of kidney allografts within 24 hours post transplant period: There was no significant difference between CMD of kidney allorgrafts with complication and kidney allografts without any complication within 24 hour because the standard deviations of both groups were 0. Corticomeduallry differentiation in all kidney allografts, all were shows maintained corticomedullary differentiation. Conclusion: This study is aimed to assessing the role of CDUS in kidney allograft recipients to evaluate the graft perfusion immediate after anastamosis, within 24 hours and follow up period after engraftment of kidney allograft. Analyse the Color Doppler Ultrasound (CDUS) indices changes in parenchymal, and vascular cause of allograft dysfunction at follow up periods, to evaluate the uroloical, surgical and vascular complications in kidney recipients by using gray scale and color Doppler US at follow up periods and compare the results with biochemical parameter (serum creatinine). Immediate Doppler ultrasound is highly useful in the diagnosis of primary graft dysfunction and in follow-up of the transplanted patient. Keywords: Graft Perfusion, Anastamosis, Color, Doppler, Ultrasound

100 Relationship between corpus luteum blood flow evaluated via colour Doppler ultrasound and pregnancy rate in bovine embryo transfer recipients

2020 ◽  
Vol 32 (2) ◽  
pp. 176
Author(s):  
M. K. Sermersheim ◽  
B. R. Lindsey ◽  
L. M. Naves ◽  
M. Rubessa ◽  
M. B. Wheeler
Keyword(s):  
Blood Flow ◽  
Medical Imaging ◽  
Corpus Luteum ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Doppler Ultrasound ◽  
Doppler Imaging ◽  
Bovine Embryo ◽  
Colour Doppler ◽  
Colour Doppler Ultrasound

The advent of colour Doppler ultrasound has allowed evaluation of blood flow to bodily tissues. This novel technology is being tested as a tool to improve efficiency in a variety of livestock reproduction programs. The objective of the present study was to evaluate the relationship between blood flow to the ovarian corpus luteum (CL), imaged via colour Doppler ultrasound, and pregnancy rate in crossbred dairy recipients for embryo transfer (ET). Oestrous cycles of Bos indicus×Bos taurus dairy heifers (n=90, 16-24 months of age) were synchronized for embryo transfer on Days 7-8. Immediately before ET, heifers were palpated for the presence of ovarian CL and CL papillae. Presence of CL, CL papillae, and CL lacunae were confirmed via transrectal B-mode ultrasound (Ibex EVO I, E. I. Medical Imaging). Transrectal colour Doppler ultrasound (Ibex EVO I, E. I. Medical Imaging) was used to evaluate blood flow to the CL. Invitro-produced (IVP) blastocysts (qualities I and II) were transferred to the uterine horn ipsilateral to the CL. All palpation, imaging, and embryo transfer was performed by a single technician. Pregnancy was determined via transrectal ultrasound 24-32 days post-embryo transfer (31-39 days of embryo age). Blood flow to the CL was split into three categories: high, median, and low. Presence or absence of two additional CL structures, CL papillae and lacunae, were recorded. Fisher's exact test was used to evaluate data. Statistical differences were considered significant at P&lt;0.05. The overall pregnancy rate was determined to be 40%, 36 out of 90. Pregnancy rates were similar in high (33.9%, 20 of 59), median (59.09%, 13 of 22), and low (33.33%, 3 of 9) blood flow categories. Consistent with previous studies, absence of CL papillae and presence of CL lacunae at the time of ET did not affect pregnancy rate. Doppler blood flow, papillae, and lacunae were also evaluated together in each possible combination. Only one outlier was present; all other differences were not significant. The highest pregnancy rate (66.67%, 10 of 15) was observed in recipients with CLs with median Doppler blood flow, a palpable CL papillae, and no CL lacunae. A larger sample size is required to determine the accuracy of this measurement. In conclusion, recipients with high to low CL blood flow are suitable for IVP embryo transfer programs. Alone, colour Doppler imaging of the CL does not predict pregnancy rate.

scholarly journals Diagnosis of Interventional Transvaginal Maternal Diseases Based on Color Doppler Ultrasound

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Canliang Wen ◽  
Lan Huang ◽  
Hongye Jiang
Keyword(s):  
Ectopic Pregnancy ◽  
Corpus Luteum ◽  
Doppler Ultrasound ◽  
Color Doppler ◽  
Color Doppler Ultrasound ◽  
Clinical Analysis ◽  
Clinical Value ◽  
Inspection Method ◽  
Ultrasound Technology

In recent years, with the development of color Doppler ultrasound technology in obstetrics, this noninvasive, direct, convenient, and sensitive inspection method has become one of the best methods to observe the fetal circulation in the uterus. This paper discusses the clinical value of using transvaginal color Doppler ultrasound in the differential diagnosis of ovarian corpus luteum disease and ectopic pregnancy disease. This paper selects 100 cases of ectopic pregnancy and 100 cases of pregnant corpus luteum as the experimental research objects. Clinical analysis of transvaginal color Doppler ultrasonography was performed on all patients. In the process of measuring the patient’s ectopic pregnancy, the size of the patient’s adnexal mass is mainly measured, and the blood flow spectrum is measured. The clinical choice of transvaginal color Doppler ultrasound method to distinguish ectopic pregnancy disease and corpus luteum pregnancy disease can play a significant value. It can be effectively diagnosed according to the type of disease, then effective methods can be studied for clinical treatment, the quality of life of patients with the two diseases can be significantly improved, and the clinical application value of color Doppler ultrasound can be improved.

scholarly journals Corpus Luteum Color Doppler Ultrasound and Pregnancy Outcome in Buffalo during the Transitional Period

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1181
Author(s):  
Luigi Esposito ◽  
Angela Salzano ◽  
Marco Russo ◽  
Donato de Nicola ◽  
Alberto Prandi ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Pregnancy Outcome ◽  
Color Doppler ◽  
Linear Regression Analysis ◽  
Strong Relationship ◽  
Color Doppler Ultrasound ◽  
Transitional Period ◽  
Flow Parameters ◽  
Significant Difference ◽  
Mean Time

This study evaluated corpus luteum (CL) development in buffaloes out of breeding season and assessed an early pregnancy diagnosis. Mediterranean buffaloes (n = 29) were synchronized and artificially inseminated. CL B-mode/color Doppler ultrasonography examinations were performed daily from Days 5 to 10 post-synchronization, recording CL dimensions and blood flow parameters. Blood samples were collected on the same days for the progesterone (P4) assay. Data were grouped into pregnant or nonpregnant and retrospectively analyzed. The total pregnancy rate was 50.0% (13/26) on Day 45. A significant difference between CL average area in pregnant and nonpregnant buffaloes was recorded only on Day 10. Pregnant buffaloes showed a significantly higher mean P4 concentration and higher mean time average medium velocity (TAMV) values from Day 5 to Day 10 compared to nonpregnant buffaloes. Linear regression analysis showed a significant relationship between P4 levels and TAMV. Multiple logistic regression highlighted a significant influence of TAMV on pregnancy outcome, particularly on Day 8. This is probably due to the strong relationship between TAMV and P4 production. Both TAMV and P4 could be used to predict pregnancy starting on Day 6, although a more reliable result was obtained at Day 10. Thus, the period between Days 5 and 10 is critical for CL development during the transitional period in buffalo.

Correlation of N-Ras Gene Expression in Hepatocellular Carcinoma with Color Doppler Ultrasound Findings

2021 ◽  
Vol 11 (10) ◽  
pp. 1996-2000
Author(s):  
Yuguo Wei ◽  
Donglai Lv
Keyword(s):  
Gene Expression ◽  
Hepatocellular Carcinoma ◽  
Western Blotting ◽  
Color Doppler ◽  
Color Doppler Imaging ◽  
Color Doppler Ultrasound ◽  
Doppler Imaging ◽  
Ras Gene ◽  
Significant Difference ◽  
Tissue Specimens

Objective: This study aims to study the N-ras gene expression in hepatocellular carcinoma (HCC) and its correlation with color Doppler imaging manifestations. Methods: The tumors of 145 HCC patients were evaluated via color Doppler ultrasonography before operation and N-ras level in tissue specimens obtained after operation were detected via RT-PCR, Western blotting and immunohistochemistry along with correlation analysis. Results: N-ras mRNA in para-carcinoma tissues was significantly reduced compared with carcinoma tissues (P < 0.05). Western blotting and immunohistochemistry showed a significant difference of N-ras protein level between para-carcinoma tissues and carcinoma tissues (P < 0.05). Moreover, N-ras gene expression was siginificantly correlated with the number of tumor nodules, the integrity of tumor capsule, vascular invasion, blood flow classification, RI (P < 0.05). Conclusion: Our data demonstrate the elevation of N-ras in the carcinoma tissues of HCC patients and its correlation with color Doppler imaging manifestations, which lays foundation for the development of the diagnosis of hepatocellular carcinoma.

Sign in / Sign up

Export Citation Format

Share Document