In Situ Comparative Studies of Self-Assembly Adsorption of Bovine Serum Albumin on Nano Films by Atomic Force Microscopy

2011 ◽  
Vol 11 (12) ◽  
pp. 10765-10769 ◽  
Author(s):  
Xuesong Ye ◽  
Yong Zeng ◽  
Tao Zhou ◽  
Feng Liu ◽  
Jun Liu ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Self Assembly ◽  
Comparative Studies ◽  
Bovine Serum ◽  
Force Microscopy ◽  
Atomic Force

Bovine serum albumin film as a template for controlled nanopancake and nanobubble formation: In situ atomic force microscopy and nanolithography study

2012 ◽  
Vol 94 ◽  
pp. 213-219 ◽  
Author(s):  
Viliam Kolivoška ◽  
Miroslav Gál ◽  
Magdaléna Hromadová ◽  
Štěpánka Lachmanová ◽  
Hana Tarábková ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Force Microscopy ◽  
Atomic Force

Bovine Serum Albumin Adhesion Force Measurements Using an Atomic Force Microscopy

2008 ◽  
Vol 32 ◽  
pp. 49-52 ◽  
Author(s):  
Chun Chih Lai ◽  
John M. Bell ◽  
Nunzio Motta
Keyword(s):  
Atomic Force Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Adhesion Force ◽  
Direct Method ◽  
Substrate Surface ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force

A new, direct method has been developed to measure the adhesion forces of bovine serum albumin (BSA) on surfaces by using Atomic Force Microscopy (AFM) in liquid environment. We were able to measure interactions between proteins and substrate surface in PBS solution directly without any modification to the substrate or the AFM tip. Two different surfaces have been used in the experiments: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The results show that a polystyrene surface is more adhesive to BSA than a mica surface. This is consistent with previous research, which assessed that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not, demonstrating the effectiveness of the technique.

scholarly journals ATOMIC FORCE MICROSCOPY MEASUREMENTS OF BOVINE SERUM ALBUMIN ADHESION FORCES ON SURFACES

2008 ◽  
Vol 07 (06) ◽  
pp. 299-303 ◽  
Author(s):  
CHUN-CHIH LAI ◽  
NUNZIO MOTTA ◽  
JOHN M. BELL
Keyword(s):  
Atomic Force Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Hydrophobic Surface ◽  
Adhesion Forces ◽  
Liquid Environment ◽  
Force Microscopy ◽  
Atomic Force ◽  
Direct Technique

A novel, direct technique has been developed to measure the interactions of bovine serum albumin (BSA) on surfaces by using atomic force microscopy (AFM) in a liquid environment. We have been able to measure adhesion forces between proteins and substrate surfaces in phosphate-buffered saline (PBS) solution directly, without any modification to the substrate and the AFM tip. Two different surfaces have been used in the measurements: mica (hydrophilic surface) and polystyrene (hydrophobic surface). The results show that a polystyrene surface has larger adhesion forces to BSA than a mica surface. This is consistent with previous research, which demonstrated that hydrophobic surfaces enhance protein adhesion but hydrophilic surfaces do not.

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