Artificial Biomimicking Matrix Modifications of Nanofibrous Scaffolds by hE-Cadherin-Fc Fusion Protein to Promote Human Mesenchymal Stem Cells Adhesion and Proliferation

To effectively expand human mesenchymal stem cells (hMSCs) in vitro without affecting their innate biological properties, a fusion protein (hE-cad-Fc) was fabricated and used as a biomimetic matrix for MSC culture surface modification.

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Trans-differentiation of human mesenchymal stem cells generates functional hepatospheres on poly(l-lactic acid)-co-poly(ε-caprolactone)/collagen nanofibrous scaffolds

Journal of Materials Chemistry B ◽

10.1039/c3tb20241k ◽

2013 ◽

Vol 1 (32) ◽

pp. 3972 ◽

Cited By ~ 45

Author(s):

Dillip Kumar Bishi ◽

Santosh Mathapati ◽

Jayarama Reddy Venugopal ◽

Soma Guhathakurta ◽

Kotturathu Mammen Cherian ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Lactic Acid ◽

Human Mesenchymal Stem Cells ◽

Nanofibrous Scaffolds

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Engineering mesenchymal stem cells with neutralizing and anti- inflammation dual-capability against SARS-CoV-2 infection

10.21203/rs.3.rs-32959/v1 ◽

2020 ◽

Author(s):

Xiaoqing Zhang ◽

Ping Han ◽

Haiyong Wang ◽

Yanqin Xu ◽

Fanlin Li ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Fusion Protein ◽

Inhibitory Effect ◽

The Novel ◽

Cytokine Storm ◽

Angiotensin Converting Enzyme 2 ◽

Fc Fusion Protein ◽

Potent Inhibitory Effect ◽

Signal Activation

Abstract The emergence of the novel human coronavirus SARS-CoV-2 has caused a worldwide epidemic of coronavirus disease 2019 (COVID-19), which markedly affected the global health and economy. Both uncontrolled viral replication and proinflammatory cytokine storm can cause severe tissue damage in COVID-19 patients. SARS-CoV-2 utilizes angiotensin-converting enzyme 2 (ACE2) as its entry receptor. In this study, we generated ACE2 extracellular domain-Fc and scFv-IL6R-Fc fusion protein to differentially neutralize virus and temper cytokine storm. The hACE21-740-Fc fusion protein showed potent inhibitory effect on pseudotyped SARS-CoV-2 entry and good safety profile in mice. The scFv-IL6R-Fc showed strong blockade effect on IL-6 signal activation. In addition, we established a mesenchymal stem cells (MSCs)-based hACE21-740-Fc and scFv-IL6R-Fc delivery strategy, which provided a potential rapid option for urgent clinic therapeutic need of COVID-19 patients.

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Evaluation of Human Mesenchymal Stem Cells Differentiation to Neural Cells on Polycaprolactone Nanofiber Scaffolds

Journal of Human Genetics and Genomics ◽

10.5812/jhgg.111835 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Seyedeh Sara Karimian ◽

Saeid Kaviani ◽

Masoud Soleimani

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Growth Factor ◽

Human Mesenchymal Stem Cells ◽

Mesenchymal Cells ◽

Neural Cells ◽

Differentiation Potential ◽

Nanofibrous Scaffolds ◽

Scanning Electron

: Differentiation of human mesenchymal stem cells (hMSC) to neural cells on Nano-scaffolds is a promising method for the treatment of the damaged nervous system through bionanomaterial-cell transplantation. The hMSC’s multipotential features have been discovered in various tissue engineering researches. This investigation shows the in-vitro development and neural differentiation of hMSC in 3D and 2D environments. The 3D environment which used in this study is nanofibrous polycaprolactone (PCL). The differentiation potential of mesenchymal stem cells (MSCs) to neural cells, on the random polycaprolactone (PCL) nanofibrous scaffolds, and tissue plate was examined. Researches have proved that interaction of extracellular nanofibrous matrix with in-vivo cells, gives mechanical maintenance to the cells and plays a functional role in the control of cellular behaviour. Stem cells are developing as a fundamental tool in the evolution of tissue engineering and regenerative medication. PCL characterization was determined employing scanning electron microscopy (SEM). Agents like, retinoic acid, epidermal growth factor (EGF), fibroblast growth factor (FGF-2), and Ibmx, which they are neural inducing agents, added in DMDM/F12 to differentiate MSCs to neural cells. Reproduction of mesenchymal cells on PCL nanofibrous scaffolds and neural morphology revealed through a scanning electron microscope (SEM) and optical microscope outcomes. The differentiated mesenchymal cells on nanofibrous scaffolds express neural gene markers including; β- tubulin III and Map2 on the day of 14. Our investigation recommends the potential usage of differentiated neural cells from hMSCs on Nano-scaffolds toward the improvement of neural cells. This study conducted in 2011.

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