Study on the Effect of Composite Nanoparticles on Corneal Epithelial Cell Immune Mechanism Based on Dectin-1 Signaling Pathway

In order to investigate the role of composite nanoparticles in the immune stage, we observed the expression of R-1 in anti-mycotic infection of human corneal epithelial cells, and divided the experiment into control group, fungal stimulation group, Gw507 blocking group and Dectin-1. In the inhibitor group, the immune effect of human corneal epithelial cells was studied. Subsequently, the expression of total R-1 and phosphorylated form of p-R-1 in each experimental group was detected by western blot method. Finally, real-time quantitative PCR was used to detect the expression of factors IL-6 and IL-8. We concluded that p-R-1 was slightly expressed in the control group. After 15 minutes of fungal stimulation, the expression level in the experimental group was significantly higher than that in the blank group, and the difference was statistically significant (p < 0.05). In the blocking group of two different inhibitors, the expression of p-R-1 was lower than that in the fungal stimulation experimental group, and the difference was statistically significant (p < 0.05). Therefore, R-1 is expressed in corneal epithelial cells and the fungus exerts its antifungal effect through the Dectin-1 signaling pathway.