Initial Study of Thin-Film Derived Gold Nanoparticles (Au NPs) Surface Plasmon Resonance (SPR) Based Prostate-Specific Antigen (PSA) Detection

2020 ◽  
Vol 18 (11) ◽  
pp. 781-784
Author(s):  
M. Dhanunjaya ◽  
T. Sonklin ◽  
Pimchanok Leuasoongnoen ◽  
Sanong Suksaweang ◽  
Pattanaphong Janphuang ◽  
...  

The gold nanoparticles (Au NPs) localized surface plasmon resonance (LSPR) based detection of prostate-specific antigen (PSA) biomarker was carried out in this work. Au NPs on Si and quartz substrates were synthesized by DC magnetron sputtering method followed by conventional annealing protocol. The functionalization of antibody and PSA with Au NPs was evaluated using a simple drop-cost method. The SPR peak intensity was found to be proportional to the PSA concentration. The redshift in SPR peak position might be owing to the change in refractive index around the Au NPs with the conjugation of antibody and further additional binding of specific PSA at different concentrations.

2021 ◽  
Author(s):  
Weixue Yang ◽  
Fei Li ◽  
Huali Liu ◽  
Zhen Li ◽  
Jiaqi Zhao ◽  
...  

A photo-assisted Li−Oxygen (Li−O2) battery with Au/SnO2 (ASO) hybrid nanotubes as cathode and photocatalyst has been prepared. The localized surface plasmon resonance (LSPR) excitation of gold nanoparticles (Au NPs) can...


2021 ◽  
Vol 24 (1) ◽  
pp. 1862-1869
Author(s):  
Nhu Hoa Thi Tran ◽  
Phuong Que Do Tran ◽  
Bach Thang Phan ◽  
Hanh Kieu Thi Ta ◽  
Ngoc Xuan Dat Mai ◽  
...  

Introduction: C-reactive protein (CPR) is known as an inflammation marker related to numerous pathology. Optical biosensor based on the fluorescence dyed is widely used in diagnosis. There are still limitations on the fluorescence signal detection due to the photobleaching effect. The localized surface plasmon resonance (LSPR) performed by gold nanoparticles (Au NPs) is testified for the enhancement of photo-signal gathered from the dye molecules. Methods: In this study, Au NPs were used for their significant optical properties and biocompatibility additionally. The seed-mediated synthesis method provided stable NPs with all the essential qualities. A series of modification steps were done on a glass substrate before the bio-bonding for fluorescence-based sensing by a transmission mode (T-mode) detection system which is introduced for the first time in Viet Nam. Results: The synthetic Au NPs in nanosphere structure evinced the absorbance at a maximum wavelength is 521 nm. All the followed alterations showed the accomplishment in forming the in need linking proved through the basic analysis methods. Finally, CRP with the Alexa 488 dye was observed for average at 4.8 folds of enhancement factor compared between the Au NPs coating and non-coating substrate detected by the T-mode system. The low coefficient of variation at under 0.7% appeared for the repeatability and stability of this sensor. Conclusion: The completely modern approach of the T-mode system combined with the LSPR applied in fluorescence sensors enhanced is developed successfully. Also, the future prospect of this designed sensing method is promising by changing the materials' structures and ingredients. Keywords: LSPR, gold nanoparticles, fluorescence enhancement, C-reaction protein, optical biosensors


Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1490
Author(s):  
Enrico Gazzola ◽  
Michela Cittadini ◽  
Marco Angiola ◽  
Laura Brigo ◽  
Massimo Guglielmi ◽  
...  

Solution processed TiO2 anatase film was used as sensitive layer for H2 detection for two plasmonic sensor configurations: A grating-coupled surface plasmon resonance sensor and a localized surface plasmon resonance sensor with gold nanoparticles. The main purpose of this paper is to elucidate the different H2 response observed for the two types of sensors which can be explained considering the hydrogen dissociation taking place on TiO2 at high temperature and the photocatalytic activity of the gold nanoparticles.


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