Objective: The objective of the study was to compare cytokine levels in the vitreous body of patients
with proliferative diabetic retinopathy (PDR) undergoing posterior vitrectomy.
Patients and methods:
The study included 39 patients (39 eyes) undergoing pars plana vitrectomy (PPV). Patients
were divided into three groups: patients with proliferative diabetic retinopathy (PDR) without aflibercept
injection prior to the surgery, PDR patients administered aflibercept injection prior to the surgery, and patients
without diabetes mellitus (control group). All patients underwent a comprehensive eye examination one day before
and 3 weeks after the surgery, including measurements of: best-corrected visual acuity (BVCA) and intraocular
pressure (IOP), slit-lamp examination and spectral domain optical coherence tomography (SOCT). Concentrations
of cytokines: IL-6, IL-8, IL-12p70, TNF, IL-10, IL-1β were measured in the vitreous body of patients with
BD™ Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit.
Results:
PDR patients who received pretreatment with aflibercept injection showed significantly lower concentrations
of IL-12p70, TNF, IL-10 and IL-1β in the vitreous body compared to the control group. Meanwhile, patients
without prior aflibercept injection had a significantly higher concentration of IL-8. There was also a significant
positive correlation between IOP before PPV and IL-8 concentration in both PDR patients’ groups.
Conclusion:
Findings of our study suggest an important role of IL-8 in the development of severe PDR. Aflibercept
administration on the day before elective vitrectomy facilitated the surgery.
Role of Mitochondria Biogenesis in the Metabolic Memory Associated with the Continued Progression of Diabetic Retinopathy and Its Regulation by Lipoic Acid
